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      • KCI등재

        Transcriptome sequencing and characterization of Astragalus membranaceus var. mongholicus root reveals key genes involved in flavonoids biosynthesis

        Jianping Liang,Wenqian Li,Xiaoyun Jia,Ying Zhang,Jianping Zhao 한국유전학회 2020 Genes & Genomics Vol.42 No.8

        Background Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao is a traditional medicinal herb of Leguminosae since it contains bioactive compounds such as flavonoids, which have significant pharmacological effects on immunity and antioxidant. However, the scanty genomic and transcriptome resources of Astragalus membranaceus have hindered further exploration of its biosynthesis and accumulation mechanism. Objective This project aim to further improve our understanding of the relationship between transcriptional behavior and flavonoids content of A. mongholicus. Methods The accumulation of flavonoids and related gene expression in five different developmental stages (A: vegetative, B: florescence, C: fruiting, D: fruit ripening and E: defoliating stages) of A. mongholicus root were studied by combining UV spectrophotometry and transcriptomic techniques. The de novo assembly, annotation and functional evaluation of the contigs were performed with bioinformatics tools. Results After screening and assembling the raw data, there were a total of 158,123 unigenes with an average length of 644.89 bp were finally obtained, which has 8362 unigenes could be jointly annotated by NR, SwissProt, eggNOG, GO, KEGG and Pfam databases. KEGG enrichment analysis was performed on differentially expressed genes(DEGs)in the four groups (A vs. B, B vs. C, C vs. D, D vs. E). The results showed that many DEGs in each group were significantly enriched to flavonoids biosynthesis related pathways. Among them, a number of 86 were involved in the biosynthesis of isoflavonoid (12), flavonoid (5) and phenylpropanoid (69). Further analysis of these DEGs revealed that the expression levels of key genes such as PAL, 4CL, CCR , COMT, DFR, etc. were all down-regulated at the fruiting stage, and then raised at the fruit ripening stage. This expression pattern was similar to the accumulation trend of total flavonoids content. Conclusions In summary, this comprehensive transcriptome dataset allowed the identification of genes associated with flavonoids metabolic pathways. The results laid a foundation for the biosynthesis and regulation of flavonoids. It also provided a scientific basis for the most suitable harvest time and resource utilization of A. mongholicus.

      • HOW CAN MORALITY-IRRELEVANT INSPIRATION LEAD TO MORALITY BEHAVIOR?

        Jianping Liang,Zengxiang Chen,Jing Lei 글로벌지식마케팅경영학회 2018 Global Marketing Conference Vol.2018 No.07

        In this paper, we investigate whether and how morality irrelevant inspiration can influence consumer’s own morality behavior. Thrash & Elliot (2003) conceptualized inspiration as a psychological construct with three core characteristics: evocation, transcendence, and motivation. Morality behavior include prescriptive morality (e.g., prosocial behavior) and proscriptive morality (e.g., immoral behavior), which have different antecedents. We propose a common antecedent for both faces of morality and found that morality-irrelevant inspirational experiences (triggered by others or oneself) could decrease proscriptive morality and increase prescriptive morality, via emotional and cognitive transcendence. This is the first paper to explore the roles played by the emotional and cognitive transcendence resulted from the inspirational experiences and the consequences of transcendence on a source-irrelevant context.

      • KCI등재

        Effects of spherical adsorbent fluidization and self-rotation on removal of VOCs in a cyclonic fluidized bed

        Liang Ma,Guolin Xiang,Yuan Huang,Meng-Ya He,Jianping Li,Pengbo Fu 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.85 No.-

        Afluidized bed has the advantages of treating largeflows, intensifying mass and heat transfer, andlowering costs. This study proposed a cyclonicfluidized bed packed with spherical activated carbonadsorbents for volatile organic compounds adsorption. Thefluidization and self-rotation of the ACparticles in a 25 mm cyclonicfluidized bed were studied with high-speed camera testing technology. Theeffects of the particle movement on the adsorption efficiency of toluene were also tested. The resultsshow that most of the particles at the inlet side of the cyclonicfluidized bed were moving up when theinlet airflow rate was greater than 2.0 m3/h. The particles began to move in clusters when the relativepacking height increased to a critical value of 0.57. Increasing the gasflow rate and the diameter andheight of the core column will increase the self-rotation speed of the total particles. The maximum selfrotationspeed of spherical adsorbents reached 1700 rad/s at the inletflow rate of 2.5 m3/h. In the case ofthe same axial velocity of the gas phase in the upper space of the core column, increasing the particle selfrotationspeed can slightly improve the adsorption efficiency. The maximum adsorption efficiencyreached 99% when the inletflow rate is 1.0 m3/h with relative packing height 0.65.

      • KCI등재

        Protective effect of quercetin against myocardial ischemia as a Ca2+ channel inhibitor: involvement of inhibiting contractility and Ca2+ infl ux via L-type Ca2+ channels

        Yingran Liang,Yuanyuan Zhang,Miaomiao Liu,Xue Han,Jianping Zhang,Xuan Zhang,Li Chu 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.8

        Many studies describe the stimulating eff ect ofquercetin on Ca 2+ channels and the treatment of cardiovasculardiseases such as myocardial ischemia and hypertension. However, these studies are scattered and contradictory. The aim of this study is to elucidate the protective eff ects ofquercetin against isoproterenol (ISO)-induced myocardialischemia and verify the cellular mechanisms based on theL-type Ca 2+ channel (LTCC), Ca 2+ transients, and myocardialcontractility. An animal model of myocardial ischemiawas established by subcutaneous injection of ISO for 2 days. Quercetin significantly reduced J-point elevation, heartrate, reactive oxygen species, serum levels of myocardialenzymes, superoxide dismutase, catalase, glutathione, glutathioneperoxidase, glutathione S-transferase and improvedheart pathologic morphology. L-type Ca 2+ current (I Ca-L )was tested in an experiment with isolated rat myocardialcells by using the whole-cell patch-clamp recording techniqueand IonOptix Myocam detection system. Quercetin reduced I Ca-L in a concentration-dependent fashion with ahalf-maximal inhibitory concentration of 4.67 × 10 –4 M. Quercetin also shifted the current–voltage curve upwards,moved the activation and inactivation curves to the left andinhibited the amplitude of the cell shortening and Ca 2+ transients. The results showed that quercetin acts as a LTCCinhibitor and exerts a cardioprotective eff ect by inhibitingCa 2+ infl ux and contractility in rats.

      • KCI등재

        Tissue-associated profiling of gene expression in the fifth-instar larvae of Chinese Oak Silkworm, Antheraea pernyi

        Duan Jianping,Liang Shimei,Zhu Zhenni,Yang Xinfeng,Li Ying,Xu Xin,Wang Jiazhen,Zhu Xuwei,Yao Lunguang 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

        Antheraea pernyi is an edible insect with important economic value in both traditional and non-traditional ap plications. Elucidating the details of gene expression variation among different A. pernyi larval tissues will greatly improve our knowledge about A. pernyi biology and promote its protection and breeding. Here, tissue-based transcriptome and gene co-expression network analysis were performed for the fifth-instar larval tissues of testis, midgut, Malpighian tubule, head, trachea, silk gland, integument, hemocyte, ovary, and fat body. A total of 1,991 tissue-associated genes, including 62 transcription factor-encoding genes, were correlated with the development and functions of these tissues. Moreover, 10 gene co-expression modules that were highly corre lated with the tissues were also determined. The most important 30 genes with top connectivity in each module were identified as hub genes to form the core co-expression network with important biological functions in the corresponding tissues. Based on these resources, further in-depth studies should be conducted to determine the roles of candidate genes and transcription factors governing tissue development and trait formation in A. pernyi.

      • KCI등재

        Flexural behaviour of reinforced concrete beams strengthened with NSM CFRP prestressed prisms

        Jiong-Feng Liang,Deng Yu,Shengjun Xie,Jianping Li 국제구조공학회 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.62 No.3

        The behaviour of reinforced concrete beams strengthened with near surface mounted (NSM) CFRP prestressed prisms was experimentally investigated. Five RC beams were tested under four point bending. All beams were made with dimensions of 300 mm in width, 2000 mm in length and 150 in depth. The effects of presstress level of CFRP prestressed prisms and prism material type were studied. The failure mode, load capacity, deflection, CFRP strain, steel strain and ductility of the tested beams were all analyzed. The results showed that the behavior of the reinforced concrete beams strengthened with NSM CFRP prestressed prisms showed a significant increase in the load-carrying capacity and the deformation capacity. The NSM CFRP prestressed prisms strengthening technique could be considered as an effective method for repairing RC structures.

      • KCI등재

        Power Cable Ampacity and Influential Factors Analysis under Operation

        ( Qiang Tong ),( Jianping Qi ),( Yanling Wang ),( Likai Liang ),( Xiangxing Meng ),( Qiang Zhang ) 한국정보처리학회 2018 Journal of information processing systems Vol.14 No.5

        With the increasing of urban electricity demand, making the most use of the power cable carrying capacity has become an important task in power grid system. Contrary to the rated ampacity obtained under extremely conservative conditions, this paper presents the various steady value of cable ampacity by using the changing surrounding parameters under operation, which is based on cable ampacity calculation equation under the IEC-60287 standard. To some degree, the cable ampacity analysis of actual surroundings improves the transmission capacity of cables. This paper reveals the factors that influence cable ampacity such as insulating layer thickness, allowable long-term conductor temperature, the ambient temperature, soil thermal resistance coefficient, and so on, then gives the class of the influence of these parameters on the ampacity, which plays a great role in accurately calculating the real-time ampacity and improving the utilization rate of cable in the complex external environment condition. Furthermore, the transient thermal rating of the cable is analyzed in this paper, and temperature variation of the conductor under different overload conditions is discussed, which provides effective information for the operation and control of the system.

      • SCOPUSKCI등재

        Power Cable Ampacity and Influential Factors Analysis under Operation

        Tong, Qiang,Qi, Jianping,Wang, Yanling,Liang, Likai,Meng, Xiangxing,Zhang, Qiang Korea Information Processing Society 2018 Journal of information processing systems Vol.14 No.5

        With the increasing of urban electricity demand, making the most use of the power cable carrying capacity has become an important task in power grid system. Contrary to the rated ampacity obtained under extremely conservative conditions, this paper presents the various steady value of cable ampacity by using the changing surrounding parameters under operation, which is based on cable ampacity calculation equation under the IEC-60287 standard. To some degree, the cable ampacity analysis of actual surroundings improves the transmission capacity of cables. This paper reveals the factors that influence cable ampacity such as insulating layer thickness, allowable long-term conductor temperature, the ambient temperature, soil thermal resistance coefficient, and so on, then gives the class of the influence of these parameters on the ampacity, which plays a great role in accurately calculating the real-time ampacity and improving the utilization rate of cable in the complex external environment condition. Furthermore, the transient thermal rating of the cable is analyzed in this paper, and temperature variation of the conductor under different overload conditions is discussed, which provides effective information for the operation and control of the system.

      • KCI등재

        Association between Metabolic Syndrome and Microvascular Complications in Chinese Adults with Type 1 Diabetes Mellitus

        Qianwen Huang,Daizhi Yang,Hongrong Deng,Hua Liang,Xueying Zheng,Jinhua Yan,Wen Xu,Xiangwen Liu,Bin Yao,Sihui Luo,Jianping Weng 대한당뇨병학회 2022 Diabetes and Metabolism Journal Vol.46 No.1

        Background: Both type 1 diabetes mellitus (T1DM) and metabolic syndrome (MetS) are associated with an elevated risk of morbidity and mortality yet with increasing heterogeneity. This study primarily aimed to evaluate the prevalence of MetS among adult patients with T1DM in China and investigate its associated risk factors, and relationship with microvascular complications.Methods: We included adult patients who had been enrolled in the Guangdong T1DM Translational Medicine Study conducted from June 2010 to June 2015. MetS was defined according to the updated National Cholesterol Education Program criterion. Logistic regression models were used to estimate the odds ratio (OR) for the association between MetS and the risk of diabetic kidney disease (DKD) and diabetic retinopathy (DR).Results: Among the 569 eligible patients enrolled, the prevalence of MetS was 15.1%. While female gender, longer diabetes duration, higher body mass index, and glycosylated hemoglobin A1c (HbA1c) were risk factors associated with MetS (OR, 2.86, 1.04, 1.14, and 1.23, respectively), received nutrition therapy education was a protective factor (OR, 0.46). After adjustment for gender, age, diabetes duration, HbA1c, socioeconomic and lifestyle variables, MetS status was associated with an increased risk of DKD and DR (OR, 2.14 and 3.72, respectively; both P<0.05).Conclusion: Although the prevalence of MetS in adult patients with T1DM in China was relatively low, patients with MetS were more likely to have DKD and DR. A comprehensive management including lifestyle modification might reduce their risk of microvascular complications in adults with T1DM.

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