1LJ-5 Active Plasmon Switching

( Jianfang Wang ) 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.1

Colloidal plasmonic nanocrystals have proven to be of enormous potential in a wide range of applications. Recently, active plasmon control has received intensive attention. Successful attempts to achieve plasmonic switching have been demonstrated by integrating metal nanostructures with active media whose dielectric functions can be varied by external stimuli. Electroactive materials are particularly appealing, because they allow for a fast switching of plasmon under electrochemical stimuli, opening the door to technological applications in electrochromic smart windows, three-dimensional and flat-panel displays. We have been able to coat polyaniline onto Au nanocrystals in controllable shell thicknesses. The plasmon resonance of the Au core can be modulated reversibly by varying the dielectric function of the polyaniline shell through proton doping and dedoping. A scattering intensity modulation depth of ~10 dB is obtained for the plasmonic switching on the single core@shell nanostructures. The plasmonic switching is accompanied with more than 100 nm in the reversible plasmon peak shift. We have further realized electrochemical switching. In the single-particle electrochemical switching measurements, reversible plasmonic shifts of ~20 and ~100 nm are obtained on the coated Au nanospheres and nanorods, with a remarkable stability over 200 cycles. The plasmon energy can be precisely controlled within the shift range, with the plasmonic switching time being less than 10 ms. The ensemble coated Au nanocrystals also exhibit good electrochemical plasmonic switching performances.

Functional Analysis of Wheat TaPaO1 Gene Conferring Pollen Sterility Under Low Temperature

Changping Zhao,Guoliang Yuan,Yukun Wang,Shaohua Yuan,Peng Wang,Wenjing Duan,Jianfang Bai,Hui Sun,Na Wang,Fengting Zhang,Liping Zhang 한국식물학회 2018 Journal of Plant Biology Vol.61 No.1

Thermosensitive male sterility plays an important role in wheat fertility and production. As a key enzyme for chlorophyll degradation, pheophorbide a oxygenase (PaO) can suppress cell death in plants. We cloned the wheat gene TaPaO1 from the thermosensitive genetic male sterile (TGMS) line BS366; it encodes a typical PaO protein, containing a conserved Rieske [2Fe-2S] iron–sulphur motif, a mononuclear non-heme iron-binding motif, and a C-terminal CxxC motif. TaPaO1 was expressed in all tissues and was upregulated during the meiosis stage of BS366 anthers under low temperature. Subcellular localization of TaPaO1 specifically labelled the surrounding of chloroplasts. TaPaO1 regulated by RD29A promoter which responded to low temperature led to pollen sterility in transgenic tobacco. Expression analysis showed that TaPaO1 exhibited a higher level of expression in the anther than in other tissues in transgenic tobacco plants during low temperature treatment. We propose that the higher senescence-related activity of TaPaO1 may lead to the cell death of anthers, which happens at an early developmental stage under low temperature. These results provide new insights into the function of PaO during the early developmental stage of anthers. PaO is closely related to cell death regardless of whether it exhibits increased activity or inactive.

Differentiation in Nitrogen-Converting Activity and Microbial Community Structure between Granular Size Fractions in a Continuous Autotrophic Nitrogen Removal Reactor

( Feiyue Qian ),( Xi Chen ),( Jianfang Wang ),( Yaoliang Shen ),( Junjun Gao ),( Juan Mei ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.10

The differentiations in nitrogen-converting activity and microbial community structure between granular size fractions in a continuous completely autotrophic nitrogen removal over nitrite (CANON) reactor, having a superior specific nitrogen removal rate of 0.24 g/(g VSS·d), were investigated by batch tests and high-throughput pyrosequencing analysis, respectively. Results revealed that a high dissolved oxygen concentration (>1.8 mg/l) could result in efficient nitrite accumulation with small granules (0.2-0.6 mm in diameter), because aerobic ammonium-oxidizing bacteria (genus Nitrosomonas) predominated therein. Meanwhile, intermediate size granules (1.4-2.0 mm in diameter) showed the highest nitrogen removal activity of 40.4 mg/(g VSS·h) under sufficient oxygen supply, corresponding to the relative abundance ratio of aerobic to anaerobic ammonium-oxidizing bacteria (genus Candidatus Kuenenia) of 5.7. Additionally, a dual substrate competition for oxygen and nitrite would be considered as the main mechanism for repression of nitrite-oxidizing bacteria, and the few Nitrospira spp. did not remarkably affect the overall performance of the reactor. Because all the granular size fractions could accomplish the CANON process independently under oxygen limiting conditions, maintaining a diversity of granular size would facilitate the stability of the suspended growth CANON system.

FGD5-AS1 promotes growth and mobility of trophoblast cells by regulating IGF2 via sponging miR-16-5p in pre-eclampsia

Quan Limei,Jin Yan,Wang Jianfang,Dai Xiwang,Zhu Shuli,Sheng Zengwei 대한독성 유전단백체 학회 2024 Molecular & cellular toxicology Vol.20 No.2

Background PE is a common pregnancy disease that can cause various pathologies in pregnant women. GEO microarray data revealed low expression of FGD5-AS1 in placenta tissues from patients with severe PE. As predicted by ENCORI, FGD5-AS1 sponges miR-16-5p, and IGF2, and promotes cell proliferation, differentiation, and metabolism. Objectives This study aimed to interrogate the interaction of FGD5-AS1/miR-16-5p/IGF2 axis and the mechanism of FGD5-AS1 in hypoxia-induced trophoblast cell injury. Results Our findings revealed that FGD5-AS1 and IGF2 were substantially decreased in PE patients than that in normal pregnant females, whereas miR-16-5p was increased in PE patients than that in normal pregnant females. In vitro studies showed that FGD5-AS1 were reduced in trophoblast cells after hypoxia treatment, accompanied by decreased viability and increased apoptosis, also reduced invasion, migration and angiogenesis. However, FGD5-AS1 overexpression substantially reversed these effects. Further mechanistic analysis showed that FGD5-AS1 could target miR-16-5p to regulate IGF2. FGD5-AS1 promoted the expression of IGF2 by sponging miR-16-5p, thereby increasing the viability of trophoblast cells after hypoxia treatment and promoting invasion and migration. Conclusion Overall, our findings demonstrated that FGD5-AS1 promoted growth and mobility of trophoblast cells by regulating miR-16-5p/IGF2 axis in PE.

IFIT1 Expression Patterns Induced by H9N2 Virus and Inactivated Viral Particle in Human Umbilical Vein Endothelial Cells and Bronchus Epithelial Cells

Feng, Bo,Zhang, Qian,Wang, Jianfang,Dong, Hong,Mu, Xiang,Hu, Ge,Zhang, Tao Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.4

IFIT1 (also known as ISG56) is a member of the interferon-inducible protein with tetratricopeptide repeats (IFITs) family. IFITs are strongly induced by type I interferon (IFN), double-stranded RNA and virus infection. Here, we investigated IFIT1 expression in human umbilical vein endothelial cells (HUVECs) and in human bronchus epithelial cells (BEAS-2Bs) induced by the H9N2 virus and inactivated viral particle at different time points. We also investigated the effect of H9N2 virus and viral particle infection on $IFN-{\alpha}/{\beta}$ production, and assessed whether hemagglutinin or neuraminidase protein induced IFIT1 expression. Results showed that both H9N2 virus infection and viral particle inoculation induced the expression of IFIT1 at mRNA and protein levels in the two cell lines. Hemagglutinin or neuraminidase protein binding alone is not sufficient to induce IFIT1 expression. Surprisingly, the expression patterns of IFIT1 in response to H9N2 virus and viral particles in the two cell lines were opposite, and production kinetics of $IFN-{\alpha}/{\beta}$ also differed. An additional finding was that induction of IFIT1 in response to H9N2 virus infection or viral particle inoculation was more sensitive in HUVECs than in BEAS-2Bs. Our data offers new insight into the innate immune response of endothelial cells to H9N2 virus infection.

Sophisticated Plasmonic Nanomaterial as the Photo-Nanozyme for Dynamic Cancer Therapy under NIR Light

유수빈,김동하,장도협,김세훈,( Jianfang Wang ) 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0

The utilization of nanomaterials as the nanozyme for dynamic cancer therapy received significant attention from the scientific community. Especially, the noble plasmonic nanomaterials have huge potential due to the intrinsic properties of noble metals, localized surface plasmon resonance (LSPR), and photothermal effect to not only induce the generation of reactive oxygen species (ROS) but also generate enough heat for cancer therapy. Herein, we synthesized uniform gold nanobipyramids, with selective deposition of palladium nanoparticles at the end. Upon laser irradiation, we observed significantly enhanced peroxidase-like activities of our materials due to facile collection of induced hot carriers at the incorporated Pd centers. Also, LSPR induced thermal effect was incorporated to our system to induce synergistic photothermal and dynamic therapy. Our work highlights the ability of this novel and highly efficient hybrid nanocomposite as a NIR-responsive tumor catalytic therapy.

IFIT1 Expression Patterns Induced by H9N2 Virus and Inactivated Viral Particle in Human Umbilical Vein Endothelial Cells and Bronchus Epithelial Cells

Bo Feng,Qian Zhang,Jianfang Wang,Hong Dong,Xiang Mu,Ge Hu,Tao Zhang 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.4

IFIT1 (also known as ISG56) is a member of the interferoninducible protein with tetratricopeptide repeats (IFITs) family. IFITs are strongly induced by type I interferon (IFN), doublestranded RNA and virus infection. Here, we investigated IFIT1 expression in human umbilical vein endothelial cells (HUVECs) and in human bronchus epithelial cells (BEAS-2Bs) induced by the H9N2 virus and inactivated viral particle at different time points. We also investigated the effect of H9N2 virus and viral particle infection on IFN-α/β production, and assessed whether hemagglutinin or neuraminidase protein induced IFIT1 expression. Results showed that both H9N2 virus infection and viral particle inoculation induced the expression of IFIT1 at mRNA and protein levels in the two cell lines. Hemagglutinin or neuraminidase protein binding alone is not sufficient to induce IFIT1 expression. Surprisingly, the expression patterns of IFIT1 in response to H9N2 virus and viral particles in the two cell lines were opposite, and production kinetics of IFN-α/β also differed. An additional finding was that induction of IFIT1 in response to H9N2 virus infection or viral particle inoculation was more sensitive in HUVECs than in BEAS-2Bs. Our data offers new insight into the innate immune response of endothelial cells to H9N2 virus infection.

Biosynthesis of Isoprenoids: Characterization of a Functionally Active Recombinant 2-C-methyl-D-erythritol 4-phosphate Cytidyltransferase (IspD) from Mycobacterium tuberculosis H37Rv

Shi, Wenjun,Feng, Jianfang,Zhang, Min,Lai, Xuhui,Xu, Shengfeng,Zhang, Xuelian,Wang, Honghai Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.6

Tuberculosis, caused by Mycobacterium tuberculosis, continues to be one of the leading infectious diseases to humans. It is urgent to discover novel drug targets for the development of antitubercular agents. The 2-C-methyl-Derythritol-4-phosphate (MEP) pathway for isoprenoid biosynthesis has been considered as an attractive target for the discovery of novel antibiotics for its essentiality in bacteria and absence in mammals. MEP cytidyltransferase (IspD), the third-step enzyme of the pathway, catalyzes MEP and CTP to form 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME) and PPi. In the work, ispD gene from M. tuberculosis H37Rv (MtIspD) was cloned and expressed. With N-terminal fusion of a histidine-tagged sequence, MtIspD could be purified to homogeneity by one-step nickel affinity chromatography. MtIspD exists as a homodimer with an apparent molecular mass of 52 kDa. Enzyme property analysis revealed that MtIspD has high specificity for pyrimidine bases and narrow divalent cation requirements, with maximal activity found in the presence of CTP and $Mg^{2+}$. The turnover number of MtIspD is $3.4 s^{-1}$. The Km for MEP and CTP are 43 and $92{\mu}M$, respectively. Furthermore, MtIspD shows thermal instable above $50^{\circ}C$. Circular dichroism spectra revealed that the alteration of tertiary conformation is closely related with sharp loss of enzyme activity at higher temperature. This study is expected to help better understand the features of IspD and provide useful information for the development of novel antibiotics to treat M. tuberculosis.

Anisotropic Gold Nanoparticles End-deposited with Pd for NIR Light Induced Tumor Catalytic Therapy

유수빈,장도협,정경화,( Jianfang Wang ),김세훈,김동하 한국공업화학회 2018 한국공업화학회 연구논문 초록집 Vol.2018 No.1

Synthesis, Fluorescence, and Antifungal Activity of a Bifunctional Lead (II) Coordination Polymer Based on Multidentate Acylhydrazone Ligand

Chengfang Qiao,Xia Gao,Qi Tang,Jianfang Wang,Yulin Gao,Meili Zhang,Zhengqiang Xia,Haitao Han,Chunxin Zhao,Chunsheng Zhou 대한화학회 2020 Bulletin of the Korean Chemical Society Vol.41 No.12

A bifunctional lead(II)-acylhydrazone coordination polymer featuring 3D hydrogen-bonded framework exhibits intense blue luminescence and remarkable inhibitory effect on the fusarium fungi.