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DNA methylation and mRNA expression of COL6A3 in antler mesenchyme of female and male reindeer
Jian‑Cheng Zhai,Ruo‑Bing Han,Sheng‑Nan Wang,Qiang‑Hui Wang,Yan‑Ling Xia,Wei‑Shi Liu,Ya‑Jie Yin,He‑Ping Li 한국유전학회 2019 Genes & Genomics Vol.41 No.9
Backgroud Reindeer is the only deer species that both male and female produce antlers, which provides a particularly interesting case in studying the differences between antlers of the two sexes. Alpha 3(VI) Collagen Gene (COL6A3), forms a microfibrillar network associated with the structural integrity and biomechanical properties, has been found to be one of the differentially expressed genes in antler mesenchyme of female and male reindeer. Objective and Methods The promoter sequence of reindeer COL6A3 gene was obtained using the cloning technology and analyzed by the bioinformatics methods. Bisulfite sequencing PCR (BSP) was used to detect the methylation status of the COL6A3 promoter in reindeer antler mesenchyme. Real-time quantitative PCR was used to detect COL6A3 expression in the antler mesenchyme of female and male reindeer. Results Sequence analysis revealed that the reindeer COL6A3 partial promoter sequence was 983 bp including the possible promoter region at + 105 bp to + 155 bp. Homology and phylogenetic analysis indicated that the COL6A3 promoter of reindeer had the closest genetic distance with Bos taurus, Capra hircus and Ovis aries. BSP results indicated that the methylation level of COL6A3 promoter in the female reindeer antler mesenchyme was significantly higher than in the male. Correlating with increased methylation status, we also found that COL6A3 mRNA expression in female reindeer antler mesenchyme was significantly lower than in the male. Conclusion The higher methylation level of the COL6A3 gene in female reindeer antler mesenchyme coincides with decreased COL6A3 mRNA expression, thereby affecting the transposon silencing mechanism and possibly contributing to apparent differences of antlers in female and male reindeer.
Li, Sheng-Cai,Dong, Jian-Xi,Li, Li-Feng Techno-Press 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.41 No.1
In order to analyze the experimental hysteretic behavior of the in-plane loaded reinforced grouted multi-ribbed aerated concrete blocks masonry walls (RGMACBMW), we have carried out the pseudo static testing on the six specimens of RGMACBMW. Based on the test results and shear failure characteristics, the shear force hysteretic curves and displacement envelope curves of the models were obtained and discussed. On the basis of the hysteretic curves a general skeleton curve of the shear force and displacement was formed. The restoring model was adopted to analyze the seismic behavior and earthquake response of RGMACBMW. The deformation capacity of the specimens was discussed, and the formulas for calculating the lateral stiffness of the walls at different loading stages were proposed as well. The average lateral displacement ductility factor of RGMACBMW calculated based on the test results was 3.16. This value illustrates that if the walls are appropriately designed, it can fully meet the seismic requirement of the structures. The quadri-linear restoring models of the walls degradation by the test results accurately reflect the hysteretic behaviors and skeleton curves of the masonry walls. The restoring model can be applied to the RGMACBMW structure in earthquake response analysis.
Sheng-Cai Li,Jian-Xi Dong,Li-Feng Li 국제구조공학회 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.41 No.1
In order to analyze the experimental hysteretic behavior of the in-plane loaded reinforced grouted multi-ribbed aerated concrete blocks masonry walls (RGMACBMW), we have carried out the pseudo static testing on the six specimens of RGMACBMW. Based on the test results and shear failure characteristics, the shear force hysteretic curves and displacement envelope curves of the models were obtained and discussed. On the basis of the hysteretic curves a general skeleton curve of the shear force and displacement was formed. The restoring model was adopted to analyze the seismic behavior and earthquake response of RGMACBMW. The deformation capacity of the specimens was discussed, and the formulas for calculating the lateral stiffness of the walls at different loading stages were proposed as well. The average lateral displacement ductility factor of RGMACBMW calculated based on the test results was 3.16. This value illustrates that if the walls are appropriately designed, it can fully meet the seismic requirement of the structures. The quadri-linear restoring models of the walls degradation by the test results accurately reflect the hysteretic behaviors and skeleton curves of the masonry walls. The restoring model can be applied to the RGMACBMW structure in earthquake response analysis.
Li, Hong-Sheng,Kong, Ling-Ling,Zhang, Jian,Li, Bao-Sheng,Chen, Jin-Hu,Zhu, Jian,Liu, Tong-Hai,Yin, Yong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5
Objectives: The purpose of this study was to evaluate the geometric accuracy of thoracic anatomic landmarks as target surrogates of intrapulmonary tumors for manual rigid registration during image-guided radiotherapy (IGRT). Methods: Kilovolt cone-beam computed tomography (CBCT) images acquired during IGRT for 29 lung cancer patients with 33 tumors, including 16 central and 17 peripheral lesions, were analyzed. We selected the "vertebrae", "carina", and "large bronchi" as the candidate surrogates for central targets, and the "vertebrae", "carina", and "ribs" as the candidate surrogates for peripheral lesions. Three to six pairs of small identifiable markers were noted in the tumors for the planning CT and Day 1 CBCT. The accuracy of the candidate surrogates was evaluated by comparing the distances of the corresponding markers after manual rigid matching based on the "tumor" and a particular surrogate. Differences between the surrogates were assessed using 1-way analysis of variance and post hoc least-significant-difference tests. Results: For central targets, the residual errors increased in the following ascending order: "tumor", "bronchi", "carina", and "vertebrae"; there was a significant difference between "tumor" and "vertebrae" (p = 0.010). For peripheral diseases, the residual errors increased in the following ascending order: "tumor", "rib", "vertebrae", and "carina"; There was a significant difference between "tumor" and "carina" (p = 0.005). Conclusions: The "bronchi" and "carina" are the optimal surrogates for central lung targets, while "rib" and "vertebrae" are the optimal surrogates for peripheral lung targets for manual matching of online and planned tumors.
Sheng-Li Shi,Jian-Xiong Li,Yi-ming Fang 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.5
This paper addresses the sliding mode control for a class of fractional order systems with matched andmismatched disturbances. Firstly, fractional disturbance observer is presented to estimate both the matched andmismatched disturbances, and the boundedness of the estimation error can be guaranteed. Secondly, sliding modesurface is constructed based on the output of the observer. The bounded stability of the closed-loop system underthe designed controller is revealed by theoretical analysis. Finally, simulation results show that the proposed controlstrategy can effectively suppress the effect of the matched and mismatched disturbances on the system.
Sliding Mode Control for Continuous Casting Mold Oscillatory System Driven by Servo Motor
Sheng-Li Shi,Ke-song Kang,Jian-Xiong Li,Yi-ming Fang 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.4
This paper develops an extended-state-observer-based sliding mode control strategy for continuous castingmold oscillatory system driven by servo motor in the presence of matched and mismatched disturbance. Extendedstate observers are employed to estimate the compound disturbances, and the requirement of the derivativeof the compound disturbances to zero is avoided. In order to counteract the mismatched disturbance and attenuatethe chattering phenomenon, a novel sliding surface based on extended state observer is designed for the system. Simulation results illustrate that the proposed controller can achieve better tracking performance and robustness tothe compound disturbance.
( Sheng Nan Li ),( Dao Sen Guo ),( Bo Guang Zhao ),( Jian Ling Ye ),( Jie Tian ),( Wen Qing Ren ),( Yun Wei Ju ),( Peng Cui ),( Rong Gui Li ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.8
A cDNA encoding a cysteine protease inhibitor (CPI) was isolated from the cDNA library of clamworm Perinereis aibuhitensis Grube. The deduced amino acid sequence analysis showed that the protein had 51%, 48%, and 48% identity with Zgc: 153129 from Danio rerio, cystatin B from Theromyzon tessulatum, and the ChainA, stefin B tetramer from Homo sapiens, respectively. The gene was cloned into the intracellular expression vector pET-15b and expressed in Escherichia coli. The recombinant CPI (PA-CPI) was purified by affinity chromatography on Nicharged resin and ion-exchange chromatography on DEAE-Sepharose FF. The relative molecular mass of PACPI was 16 kDa as deduced by SDS-PAGE. Activity analysis showed that the recombinant protein could inhibit the proteolytic activity of papain. A constitutive and secretive expression vector was also constructed, and the cDNA encoding CPI was subcloned into the vector for extracellular expression. Western blotting analysis results showed that the PA-CPI was secreted into the medium. Bioassay demonstrated that E. coli DH5α harboring pUC18ompAcat-CPI showed a significant difference in mortality to the Asian longhorned beetle Anoplophora glabripennis compared with untransformed E. coli DH5α and control.
Two New Phenolic Compounds from the Fruiting Bodies of Ganoderma tropicum
Hu, Li-Li,Ma, Qing-Yun,Huang, Sheng-Zhuo,Guo, Zhi-Kai,Guo, Jian-Chun,Dai, Hao-Fu,Zhao, You-Xing Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.3
Chemical investigation of the fruiting bodies of Ganoderma tropicum led to the isolation of two new phenolic compounds, ganodermatropins A (1) and B (2). Their structures were elucidated by spectroscopic techniques (MS, 1D and 2D NMR). Ganodermatropin A exhibited antimicrobial activity against Staphylococcus aureus.
Ding Sheng-Long,Zhang Tai-Wei,Zhang Qi-Chen,Ding Wang,Li Ze-Fang,Han Guan-Jie,Bai Jin-Song,Li Xi-Lei,Dong Jian,Wang Hui-Ren,Jiang Li-Bo 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-
Night shift workers with disordered rhythmic mechanical loading are more prone to intervertebral disc degeneration (IDD). Our results showed that circadian rhythm (CR) was dampened in degenerated and aged NP cells. Long-term environmental CR disruption promoted IDD in rats. Excessive mechanical strain disrupted the CR and inhibited the expression of core clock proteins. The inhibitory effect of mechanical loading on the expression of extracellular matrix genes could be reversed by BMAL1 overexpression in NP cells. The Rho/ROCK pathway was demonstrated to mediate the effect of mechanical stimulation on CR. Prolonged mechanical loading for 12 months affected intrinsic CR genes and induced IDD in a model of upright posture in a normal environment. Unexpectedly, mechanical loading further accelerated the IDD in an Light-Dark (LD) cycle-disrupted environment. These results indicated that intrinsic CR disruption might be a mechanism involved in overloading-induced IDD and a potential drug target for night shift workers.