Life history responses of the cabbage beetle Colaphellus bowringi to temperature change

Jian‐Jun Tang,Hai-Min HE,Ting GENG,Shu FU,Fang-Sen XUE 한국곤충학회 2016 Entomological Research Vol.46 No.6

Temperature is considered one of the most important mediators of phenotypic plasticity in ectotherms. Here, we investigated life history traits of the cabbage beetle, Colaphellus bowringi Baly (Coleoptera: Chrysomelidae), at a wide range of temperatures (16, 19, 22, 24, 26 and 28°C). The larval and pupal times were significantly decreased with increasing rearing temperature and growth rate was positively correlated with temperature. However, the relationship between body size and rearing temperature in C. bowringi did not follow the temperature–size rule; both males and females reached the highest body weight at 19°C. Females were significantly larger than males at all temperatures. Male pupae lost significantly more weight at metamorphosis compared to females. However, diapausing males gained significantly higher weight after feeding compared to diapausing females at higher temperatures of 22, 24, 26 and 28°C. Body weight tended to decrease with increasing rearing temperature, whereas sexual size dimorphism (SSD) tended to increase with increasing rearing temperature; thus, Rensch's rule is upheld. The degree to which SSD changed with temperature varied with different development stages. SSD was lowest in pupae, highest in newly emerged adults and intermediate in diapausing adults.

Temperature-dependent oviposition model of Scopula subpunctaria (Lepidoptera: Geometridae)

Geng Shubao,Hou Heli,Wang Guojun,Jung Chuleui,Yin Jian,Qiao Li 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

Scopula subpunctaria, is one of important defoliating insect pests of tea plants in China. However, the effect of temperature on its longevity and fecundity had not been reported, and the temperature-dependent oviposition model is lacking. The reproductive characteristics of S. subpunctaria were investigated under eight constant temperatures (13, 16, 19, 22, 25, 28, 31, and 33℃), with a 14L: 10D photoperid and 65–85% RH. The results showed that temperature significantly influence oviposition period, longevity, and fecundity of S. subpunctaria. The mean longevity of female adults increased from 10.8 days (33℃) to 33.9 days (13℃). Male lived shorter than female did. The mean longevity of males was shortest at 33℃ (8.1 days) and longest at 23.8℃ (17.6 days). Total fecundity of S. subpunctaria females ranged from 15.4 eggs at 33℃ to 279.9 eggs at 22℃. The temperaturedependent oviposition model of S. subpunctaria was constructed based on four sub-models (developmental rate model, total fecundity model, oviposition rate model, and survival rate model). The results of this study would be helpful in forecasting and predicting population occurrence of S. subpunctaria in tea plantations.

Energy-Aware Hybrid Cooperative Relaying with Asymmetric Traffic

Jian Chen,Lu Lv,Wenjin Geng,Yonghong Kuo 한국전자통신연구원 2015 ETRI Journal Vol.37 No.4

In this paper, we study an asymmetric two-way relaying network where two source nodes intend to exchange information with the help of multiple relay nodes. A hybrid time-division broadcast relaying scheme with joint relay selection (RS) and power allocation (PA) is proposed to realize energy-efficient transmission. Our scheme is based on the asymmetric level of the two source nodes’ target signal-to-noise ratio indexes to minimize the total power consumed by the relay nodes. An optimization model with joint RS and PA is studied here to guarantee hybrid relaying transmissions. Next, with the aid of our proposed intelligent optimization algorithm, which combines a genetic algorithm and a simulated annealing algorithm, the formulated optimization model can be effectively solved. Theoretical analyses and numerical results verify that our proposed hybrid relaying scheme can substantially reduce the total power consumption of relays under a traffic asymmetric scenario; meanwhile, the proposed intelligent optimization algorithm can eventually converge to a better solution.

Novel Method for Circulating Current Suppression in MMCs Based on Multiple Quasi-PR Controller

Jian Qiu,Lijun Hang,Dongliang Liu,Shengbao Geng,Xiaonan Ma,Zhen Li 전력전자학회 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.6

An improved circulating current suppression control method is proposed in this paper. In the proposed controller, an outer loop of the average capacitor voltage control model is used to balance the sub-module capacitor voltage. Meanwhile, an individual voltage balance controller and an arm voltage balance controller are also used. The DC and harmonic components of the circulating current are separated using a low pass filter. Therefore, a multiple quasi-proportional-resonant (multi-quasi-PR) controller is introduced in the inner loop to eliminate the circulating harmonic current, which mainly contains second-order harmonic but also contains other high-order harmonics. In addition, the parameters of the multi-quasi-PR controller are designed in the discrete domain and an analysis of the stability characteristic is given in this paper. In addition, a simulation model of a three-phase MMC system is built in order to confirm the correctness and superiority of the proposed controller. Finally, experiment results are presented and compared. These results illustrate that the improved control method has good performance in suppressing circulating harmonic current and in balancing the capacitor voltage.

Mesenchymal-to-epithelial transition of osteoblasts induced by Fam20c knockout

Geng Ya-Wei,Zhang Zhen,Jin Han,Da Jun-Long,Zhang Kai,Wang Jian-Qun,Guo Yu-Yao,Zhang Bin,Li Ying 한국유전학회 2022 Genes & Genomics Vol.44 No.2

Background: Fam20c is intimately related to tissue development and diseases. At present, it has been reported that Fam20c regulates the mineralization of osteoblasts, but there are few reports on other effects. Objective: To study the effect of Fam20c on osteoblasts by knocking out the Fam20c gene. Methods: Fam20c knockout osteoblasts were constructed by transfecting mouse osteoblasts with lentivirus. The proliferation, migration and mineralization of Fam20c knockout cells were detected by CCK-8, scratch test and alizarin red staining assays. The subcellular structure was observed by transmission electron microscopy. RT-PCR was used to detect the differential expression of mesenchymal-to-epithelial transition (MET)-related marker genes and core transcription factors. The differential expression of MET-related proteins was detected by immunofluorescence or Western blot. Transcriptome analysis of Fam20c knockout osteoblasts was performed, and real-time PCR was used to verify transcriptome analysis related to MET. Results: The proliferation ability of osteoblasts was not significantly changed after Fam20c deletion, but the migration ability and mineralization ability were significantly weakened. There were tight junctions between Fam20c knockout cells. The expression of mesenchymal cell marker genes and core transcription factors was significantly decreased, and the expression of epithelial cell marker genes was significantly increased. The expression of mesenchymal cell marker proteins was significantly decreased, and the expression of epithelial cell marker proteins was significantly increased. Multiple signalling molecules and pathways involved in MET have changed. Conclusions: Knockdown of Fam20c resulted in MET. Fam20c affects the transcription of key factors in osteoblast MET.

Fabrication of Composite Polymer Foam Films at the Liquid/Liquid Interface through Emulsion-Directed Assembly and Adsorption Processes

Geng, Yuanyuan,Liu, Mei,Tong, Kun,Xu, Jian,Lee, Yong-Ill,Hao, Jingcheng,Liu, Hong-Guo American Chemical Society 2014 Langmuir Vol.30 No.8

<P>The foam films of polystyrene-<I>b</I>-poly(acrylic acid)-<I>b</I>-polystyrene (PS-<I>b</I>-PAA-<I>b</I>-PS) doped with Cd(II) or Pb(II) species were fabricated at the planar liquid/liquid interfaces between a DMF/chloroform (v/v: 1/1) solution of the polymer and aqueous solutions containing cadmium acetate or lead acetate at ambient temperature. Optical microscopic observation shows the thin film is uniform on a larger length scale. Transmission electron microscopic (TEM) investigations reveal that the foam films are made up of microcapsules with the size of several hundreds of nanometers to micrometers. The walls of the microcapsules have a layered structure decorating with nanofibers and hollow nanospheres, where numerous inorganic fine nanoparticles are dispersed homogeneously. The film formation is a result of emulsion droplet-templated assembly and adsorption of the formed microcapsules at the planar liquid/liquid interface. Because of the miscibility of DMF with chloroform and water, DMF migrates to the aqueous phase while water migrates to the organic phase across the interface, resulting in the formation of a W/O emulsion, as revealed by optical microscopic observation, freeze fracture transmission electron microscopic (FF-TEM) observation, and dynamic laser scattering (DLS) investigation. The triblock copolymer molecules and the inorganic species adsorb and self-assemble around the emulsion drops, leading to the formation of the composite microcapsules. X-ray photoelectron spectroscopic (XPS) and FTIR spectroscopic results indicate that two kinds of Cd(II) or Pb(II) species, metal oxide or hydroxide, resulting from the hydrolysis of the metal ions and the coordinated metal ions to the carboxyl groups coexist in the formed thin films, which transform to metal sulfide completely after treating with hydrogen sulfide to get metal sulfide nanoparticle-doped polymer thin films.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/langd5/2014/langd5.2014.30.issue-8/la500187d/production/images/medium/la-2014-00187d_0012.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/la500187d'>ACS Electronic Supporting Info</A></P>

Preparation and Characterization of Paclitaxel-Loaded PLGA Nanoparticles Coated with Cationic SM5-1 Single-chain Antibody

( Geng Kou ),( Jie Gao ),( Hao Wang ),( Huai Wen Chen ),( Bo Hua Li ),( Da Peng Zhang ),( Shu Hui Wang ),( Sheng Hou ),( Wei Zhu Qian ),( Jian Xin Dai ),( Yan Qiang Zhong ),( Ya Jun Guo ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.5

Novel Method for Circulating Current Suppression in MMCs Based on Multiple Quasi-PR Controller

Qiu, Jian,Hang, Lijun,Liu, Dongliang,Geng, Shengbao,Ma, Xiaonan,Li, Zhen The Korean Institute of Power Electronics 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.6

An improved circulating current suppression control method is proposed in this paper. In the proposed controller, an outer loop of the average capacitor voltage control model is used to balance the sub-module capacitor voltage. Meanwhile, an individual voltage balance controller and an arm voltage balance controller are also used. The DC and harmonic components of the circulating current are separated using a low pass filter. Therefore, a multiple quasi-proportional-resonant (multi-quasi-PR) controller is introduced in the inner loop to eliminate the circulating harmonic current, which mainly contains second-order harmonic but also contains other high-order harmonics. In addition, the parameters of the multi-quasi-PR controller are designed in the discrete domain and an analysis of the stability characteristic is given in this paper. In addition, a simulation model of a three-phase MMC system is built in order to confirm the correctness and superiority of the proposed controller. Finally, experiment results are presented and compared. These results illustrate that the improved control method has good performance in suppressing circulating harmonic current and in balancing the capacitor voltage.

Clinical and Histopathological Analysis of 66 Cases with Cardiac Myxoma

Zheng, Jian-Jie,Geng, Xi-Gang,Wang, Hai-Chen,Yan, Yang,Wang, Hong-Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Background and Purpose: Cardiac myxoma is a major primary heart tumor which often causes unexpected symptoms or sudden death. This present study was designed to investigate its clinical pathological features and biological behavior. Methods: A retrospective analysis of the clinical pathologic and immunohistochemical features of 66 cases with cardiac myxoma was conducted. Results: In 66 patients with cardiac myxoma, 61 cases had involvement of the left atrium, one case in both the right ventricular and left atria. The female: male ratio was 2.7:1. Patients had symptoms of blood flow obstruction and systemic alterations with performance of arterial embolization. Tumors were spherical, lobulated or irregular in shape, and soft and brittle. Immunohistochemical markers of vimentin and CD34 in tumor cells were positive. Conclusion: Cardiac myxoma always exists in the left atrium and is more common in women, with diverse clinical manifestations and pathomorphism. Although proliferative activity and the recurrence rate are low, in addition to thorough surgical resection, strengthened review is important for young patients.

Molecular Cloning, Segmental Distribution and Ontogenetic Regulation of Cationic Amino Acid Transporter 2 in Pigs

Zou, Shi-geng,Zhi, Ai-min,Zhou, Xiang-yan,Zuo, Jian-jun,Zhang, Yan,Huang, Zhi-yi,Xu, Ping-Wen,Feng, Ding-yuan Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.5

The goal of this study was to elucidate the expression and segmental distribution of the glomerular cationic amino acid metabolism transporter-2 (CAT-2) and thus to improve our understanding of porcine cationic amino acid transporters and amino acid absorption. Porcine CAT-2 was cloned, sequenced and characterized. The predicted amino acid sequence of porcine CAT-2 shared 86.1% and 92.1% identity with human and mouse CAT-2A, respectively. The tissue distribution patterns and ontogenic changes of CAT-2 mRNAs were determined by real-time Q-PCR. The results showed that porcine CAT-2 was highly expressed in the heart and intestinal tract (duodenum, ileum and jejunum). In addition, the mRNA of CAT-2 was found in liver, lung, kidney, brain and muscle. Within the intestinal tract, CAT-2 mRNA was most abundant in the ileum and rarely expressed in the duodenum. In the duodenum, the levels of CAT-2 mRNA reached their peak on day 7 (p<0.05) while in the jejunum, levels were low on day 1 and 7 and increased rapidly after day 26 before peaking on days 30 and 60 (p<0.05). The levels then dramatically decreased by day 90 (p<0.05). In the ileum, levels achieved their maximum on day 30 and then decreased significantly on day 60 (p<0.05).