Analysis of Volatile Compounds in Chinese Soy Sauces Moromi Cultured by Different Fermentation Processes

Jia Zheng,Chong-De Wu,Jun Huang,Rong-Qing Zhou,Xue Pin Liao 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.3

The volatile compounds in Chinese soy sauce moromi cultured by different fermentation processes [lowsalt solid-state fermentation (LSSF), high-salt dilute-state fermentation (HSDF), and high-salt constant temperature fermentation (HSCT)] were determined by GC-MS. The LSSF moromi had the highest total concentration of volatile compounds, followed by HSCT and HSDF moromi. Volatiles such as ethyl linoleate and ethyl oleate dominated in HSCT moromi. 2,3-Butanediol and 2,6-dimethylpyrazine were present at high content in LSSF moromi. The hierarchal cluster analysis (HCA) and principal component analysis (PCA) were employed to investigate the fermentation process effect on the category of samples. Based on HCA,HSCT (a1 and a2) and HSDF (b1 and b2) clustered in 1group, and LSSF (c1 and c2) located in another group. PCA illustrated that each kind of samples correlating with specific volatile compound groups were clearly differentiated according to their fermentation processes. The results suggest that GC-MS together with multivariate analyses could provide practical reference to recognize different Chinese soy sauces moromi.

Serum Proteomics Analysis of Feline Mammary Carcinoma Based on Label-free and PRM Techniques

Jia-San Zheng,Ren-Yue Wei,Zheng Wang,Ting-Ting Zhu,Hong-Ri Ruan,Xue Wei,Kai-Wen Hou,Rui Wu 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.3

Background: Feline mammary carcinoma is the third most common cancer that affects female cats. Objectives: The purpose of this study was to screen differential serum proteins in feline and clarify the relationship between them and the occurrence of feline mammary carcinoma. Methods: Chinese pastoral cats were used as experimental animals. Six serum samples from cats with mammary carcinoma (group T) and six serum samples from healthy cats (group C) were selected. Differential protein analysis was performed using a Label-free technique, while parallel reaction monitoring (PRM) was performed to verify the screened differential proteins. Results: A total of 82 differential proteins were detected between group T and group C, of which 55 proteins were down regulated and 27 proteins were up regulated. Apolipoprotein A-I, Apolipoprotein A-II (ApoA-II), Apolipoprotein B (ApoB), Apolipoprotein C-III (ApoC-III), coagulation factor V, coagulation factor X, C1q, albumen (ALB) were all associated with the occurrence of feline mammary carcinoma. Differential proteins were involved in a total of 40 signaling pathways, among which the metabolic pathways associated with feline mammary carcinoma were the complement and coagulation cascade and cholesterol metabolism. According to the Label-free results, ApoB, ApoC-III, ApoA-II, FN1, an uncharacterized protein, and ALB were selected for PRM target verification. The results were consistent with the trend of the label-free. Conclusions: This experimen is the first to confirm ApoA-II and ApoB maybe new feline mammary carcinoma biomarkers and to analyze their mechanisms in the development of such carcinoma in feline.

Knockdown of Maternal Homeobox Transcription Factor SEBOX Gene Impaired Early Embryonic Development in Porcine Parthenotes

ZHENG, Zhong,ZHAO, Ming-Hui,JIA, Jia-Lin,HEO, Young-Tae,CUI, Xiang-Shun,OH, Jeong Su,KIM, Nam-Hyung The Society for Reproduction and Development 2013 The Journal of reproduction and development Vol.59 No.6

<P><B>Abstract</B></P><P> A number of germ cell-specific transcription factors essential for ovarian formation and folliculogenesis have been identified and studied. However, the role of these factors during early embryonic development has been poorly explored. In the present study, we investigated the role of SEBOX, a maternal homeobox transcription factor, during early embryonic development in porcine parthenotes. mRNA for <I>SEBOX</I> is preferentially expressed in oocytes, and expression persists until embryonic genome activation (EGA). Knockdown of SEBOX by siRNA disrupted early embryonic development, but not oocyte maturation. Many maternal genes essential for early embryonic development were upregulated in SEBOX-depleted embryos. Moreover, some pluripotency-associated genes, including <I>SOX2</I> and <I>NANOG</I>, were upregulated when SEBOX was knocked down. Therefore, our data demonstrate that SEBOX is required for early embryonic development in pigs and appears to regulate the degradation of maternal transcripts and the expression of pluripotency genes.</P>

Pythium subutonaiense, A New Aquatic Oomycete from Southern China Based on Morphological and Molecular Characters

( Jia-jia Chen ),( Xiao-bo Zheng ) 한국균학회 2019 Mycobiology Vol.47 No.3

A new species, Pythium subutonaiense, isolated from aquatic environments (lake) in China is being described based on morphological characters and molecular evidence. The isolates grew at temperatures between 5 ℃ and 38 ℃, and the optimum temperature was 30 ℃, with a radial growth rate of 17.6mm at 25 ℃ per day. It is homothallic and characterized by globose to sub-globose shaped and mostly terminal or sometimes catenulate hyphal swellings, filamentous non-inflated sporangia, and smooth oogonia with hypogynous and monoclinous antheridia that contained one plerotic oospore. In phylogenetic analysis, inferred based on the internal transcribed spacer region of the ribosomal RNA gene and mitochondrial cytochrome c oxidase subunit 1 gene, the new species formed a distinct lineage in Pythium clade B. Differences between the new species and phylogenetically related and morphologically similar species are discussed.

Research on the Performance Optimization of Hadoop in Big Data Environment

Jia Min-Zheng 보안공학연구지원센터 2015 International Journal of Database Theory and Appli Vol.8 No.5

In the age of Internet, the data transmission and storage got rapid progress, however, data processing and information extraction is still exist many problems to solve. Under the condition of so much data, processing data, get useful information; In cloud computing, big data environment to adopt the method of distributed computing, such a large complex networks, however, requires a simulation environment, for comparison and optimization platform, it can save development costs. Hadoop can evaluate the performance of distributed cloud computing platform, so the Hadoop performance directly affects the evaluation on the performance of the big data cloud computing, which fully show the importance of performance of Hadoop. Algorithm is improved based on Hadoop platform, using the particle swarm optimization algorithm improved the calculation and implementation of the Hadoop platform, so as to improve its ability to execute and compute, the calculation results and analysis show that the proposed scheme is effective.

Tumor-Derived Transforming Growth Factor-β is Critical for Tumor Progression and Evasion from Immune Surveillance

Li, Zheng,Zhang, Li-Juan,Zhang, Hong-Ru,Tian, Gao-Fei,Tian, Jun,Mao, Xiao-Li,Jia, Zheng-Hu,Meng, Zi-Yu,Zhao, Li-Qing,Yin, Zhi-Nan,Wu, Zhen-Zhou Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Tumors have evolved numerous mechanisms by which they can escape from immune surveillance. One of these is to produce immunosuppressive cytokines. Transforming growth factor-${\beta}$(TGF-${\beta}$) is a pleiotropic cytokine with a crucial function in mediating immune suppression, especially in the tumor microenvironment. TGF-${\beta}$ produced by T cells has been demonstrated as an important factor for suppressing antitumor immune responses, but the role of tumor-derived TGF-${\beta}$ in this process is poorly understood. In this study, we demonstrated that knockdown of tumor-derived TGF-${\beta}$ using shRNA resulted in dramatically reduced tumor size, slowing tumor formation, prolonging survival rate of tumor-bearing mice and inhibiting metastasis. We revealed possible underlying mechanisms as reducing the number of myeloid-derived suppressor cells (MDSC) and $CD4^+Foxp3^+$ Treg cells, and consequently enhanced IFN-${\gamma}$ production by CTLs. Knockdown of tumor-derived TGF-${\beta}$ also significantly reduced the conversion of na$\ddot{i}$ve $CD4^+$ T cells into Treg cells in vitro. Finally, we found that knockdown of TGF-${\beta}$ suppressed cell migration, but did not change the proliferation and apoptosis of tumor cells in vitro. In summary, our study provided evidence that tumor-derived TGF-${\beta}$ is a critical factor for tumor progression and evasion of immune surveillance, and blocking tumor-derived TGF-${\beta}$ may serve as a potential therapeutic approach for cancer.

Research on the Development Efficiency of Tourism in the Non-Pearl River Delta of Guangdong

Lin, Jia-Zheng,Kim, Hyung-Ho The International Promotion Agency of Culture Tech 2021 International Journal of Advanced Culture Technolo Vol.9 No.3

On February 18, 2019, the Chinese government officially released the Outline of the Development Plan for the Guangdong-Hong Kong-Macao Greater Bay Area, which will lead the country in a new round of reform and opening-up. The Greater Bay Area will become a dynamic world-class city cluster, an international scientific and technological innovation center with global influence, an important support for the development of the "One Belt And One Road", a demonstration area for in-depth cooperation between the mainland and Hong Kong and Macao, and a high-quality living area for living, working and traveling. Non-Pearl River Delta(Non-PRD) cities in Guangdong Province are adjacent to the Guangdong-Hong Kong-Macao Greater Bay Area, so it is of practical significance to promote the high-quality development of urban tourism from an international perspective. Based on the panel data released in Guangdong Yearbook 2019, this paper uses the envelopment data analysis (DEA) method to explore ways to promote the high-quality tourism development of Non-PRD cities in Guangdong Province based on the perspective of international development.

Maternal Homeobox Transcription Factor Sebox is Essential for Early Embryonic Development in Porcine Parthenotes

Zhong Zheng,Ming-Hui Zhao,Jia-Lin Jia,Young-Tae Heo,Xiang-Shun Cui,Jeong Su Oh,Nam-Hyung Kim 한국동물생명공학회(구 한국동물번식학회) 2013 Reproductive & Developmental Biology(Supplement) Vol.37 No.2s

중국 당조시대(唐朝時代)의 전통 건축에 나타난 식물 문양에 관한 연구

가쟁 ( Zheng Jia ),김주연 ( Joo Yun Kim ) 한국공간디자인학회 2013 한국공간디자인학회논문집 Vol.8 No.3

(연구배경 및 목적) 역사적으로 중국은 당나라 대 통일을 이루고, 서양과도 교류를 활발히 하였는데, 이로 인해 당조시대의 전통 문양에는 여러 문화와 종교의 융합이 구현되어 있다. 이에 본 논문은 중국 전통 식물 문양의 기원을 밝히고 중국의 전통 건축요소에 나타난 문양의 자료 분석을 통해 식물 문양의 역사와 발전과정을 살펴보고, 중국 당 대 전통 식물 문양의 유형과 특성에 대해 고찰하고자 하였다.(연구범위) 당 대의 식물 문양은 원형배치가 특징인 보상화문과 S 형태의 배치 구조를 가진 권초문을 주로 사용하였으며 동물, 식물, 인물 등 다양한 문양의 조합으로 생동감 있는 도안이 형성되었다. 그 중에서도 당 대의 권초문과 보상화문은 장식 문양 중 가장 중요한 자리를 잡고 있어, 이를 중심으로 문양의 발전과정과 특성에 따라 건축의 요소별 사례를 분석하였다.(결과) 당 대 식물 문양은 상징적, 미학적, 종교적인 특성을 나타내는데, 당 대 초기에는 불교를 바탕으로 단순함과 소박함을 표현하기 위해 대칭과 균형을 주로 이용하였다. 이후에 서양의 기독교와 이슬람의 영향으로 변화하기 시작하였으며 풍요로움과 부귀함을 표현하기 위해 반복과 변화, 리듬을 이용하였다. (Background and Purpose) The Tang dynasty unified China and interacted with many Western nations. This reaching out led to cross-pollination, imbuing the plant patterns of the Tang dynasty with a mixture of culture and religion. In this study, the origin, history, and evolution of plant patterns are analyzed in light of Tang dynasty architecture. In addition, we undertake a contemplative study of the types and features of Chinese traditional plant patterns. (Research) In the Tang dynasty, the Bosang-wha Moon (floral medallion motif), a circular pattern, and the Gwon-cho Moon (floral scrolls), an "S"-shape pattern, were mainly used for decorative purposes in the shape of various animals, plants, or people. Combinations of these elements gave a dynamic flow to the patterns. Because Bosang-wha Moon and Gown-cho Moon are generally in the foreground of Tang dynasty plant patterns, this study analyzes examples of various elements of Tang dynasty architecture, focusing on the evolutionary process and character of the patterns. (Result)Tang plant patterns have symbolic, artistic, and spiritual characteristics. In the early Tang dynasty, symmetric, balanced shapes were used to represent simplicity. Later, after Western and Islamic religious influences had taken hold, repetition, alternation, and rhythm were implemented to demonstrate wealth and abundance.

글리콜키토산을 이용한 초다공성 하이드로젤의 제조 및 팽윤거동

광가 ( Jia Kuang ),이정정 ( Zheng Zheng Li ),윤취임 ( Chwi Im Yun ),육군영 ( Kun Young Yuk ),허강무 ( Kang Moo Huh ) 한국유화학회 2009 한국응용과학기술학회지 Vol.26 No.3