Structural Basis of Emi2 Recognition by Polo-Box Domain of Polo-like Kinase 1 and Effects of Structure-Derived Antagonist in Oocyte Maturation and Fertillization

Jia-Jia Lin,Young-Hyun Han,Jung-Woo Kwon,Yong-Nan Xu,Yi-Bo Luo,Yu-Jin Jo,Chang-Eun Park,Jung-Kyu Baang,Suk Namgoong,Nam-Hyung Kim 한국동물생명공학회(구 한국동물번식학회) 2014 Reproductive & Developmental Biology(Supplement) Vol.38 No.2s

In meiosis, Emi2 plays important role as CSF (Cytostatic Factor) to make the oocyte arrested in mII stage by the inhibition of APC/C (anaphase promoting complex/cyclosome). Once the oocyte fertilized, Emi2 was destabilized and degraded. For the degradation of Emi2, calcium signaling activate calmodulin-dependent protein kinase (CaMK) and phosphorylate emi2. Phosphorylated emi2 is recognized by polo-box domain of polo-like kinase 1 (Plk1) and further degradated by ubiquitin-dependent proteolysis. But recognition of Plk1 and emi2 is unknown. In this works, we determined the high-resolution crystal structure of polo-box domain of Plk1 and phosphorylated emi2 peptide at 1.90Å. Determined structure revealed that several unique features, including binding of Phe169 in the tyrosin-rich hydrophobic pocket. This is the first report of crystallization that Plk1-emi2 complex. Based on the complex structure, we designed the peptide analogs which pontentially inhibits recognition of Emi2 by Plk1 and assessed its biological activity in oocyte maturation and pathernogenetic activation. Injection of AB103-8, the inhibitor of Plk1 Polo-box domain, in mouse oocytes, induced the maturation arrest in GV stage and the delay in mII parthenogenetic activation. Further investigations of the mechanism that Plk1 involved into the Emi2 mII arrest are underway.

Research on the Development Efficiency of Tourism in the Non-Pearl River Delta of Guangdong

Lin, Jia-Zheng,Kim, Hyung-Ho The International Promotion Agency of Culture Tech 2021 International Journal of Advanced Culture Technolo Vol.9 No.3

On February 18, 2019, the Chinese government officially released the Outline of the Development Plan for the Guangdong-Hong Kong-Macao Greater Bay Area, which will lead the country in a new round of reform and opening-up. The Greater Bay Area will become a dynamic world-class city cluster, an international scientific and technological innovation center with global influence, an important support for the development of the "One Belt And One Road", a demonstration area for in-depth cooperation between the mainland and Hong Kong and Macao, and a high-quality living area for living, working and traveling. Non-Pearl River Delta(Non-PRD) cities in Guangdong Province are adjacent to the Guangdong-Hong Kong-Macao Greater Bay Area, so it is of practical significance to promote the high-quality development of urban tourism from an international perspective. Based on the panel data released in Guangdong Yearbook 2019, this paper uses the envelopment data analysis (DEA) method to explore ways to promote the high-quality tourism development of Non-PRD cities in Guangdong Province based on the perspective of international development.

Bamboo Charcoal/Quick-Dry/Metallic Elastic Knits: Manufacturing Techniques and Property Evaluations

Jia-Horng Lin,Chih Hung He,Mong-Chuan Lee,Yueh-Sheng Chen,Ching-Wen Lou 한국섬유공학회 2019 Fibers and polymers Vol.20 No.7

Protective clothing with electromagnetic shielding effectiveness (EMSE) is a functional textile, which shieldselectromagnetic waves to protect the human body from the far infrared rays. Traditional EMSE garments primarily usemetallic plates for reflecting electromagnetic interference (EMI). However, they also have disadvantages of a heavy weight,easy corrosion, and high production cost. In this study, three different functional yarns are combined, during which the twistnumber of metallic wires is changed for a better shielding effect. A crochet machine is used to fabricate functional elasticknits with the metallic composite yarns as the warp- and weft-inlaid yarns. The test results show that double (D) functionalelastic knits exhibit EMSE of above 20 dB when the EMI are between 1.0 and 3.0 GHz. In addition, the knit structure canimprove breaking tensile strength by 170 % and increases bursting strength by 58 %, but undermines the tear strength by10 %. Although changing twist number of metallic composite yarns does not affect water absorption rate along the weftdirection, it helps increasing elastic recovery rate by 3.1 %, water absorption rate along the warp direction by 106 %, farinfraredemissivity by 2.3 %, water vapor transmission rate by 20 %, and air permeability by 6 %.

Dexmedetomidine Attenuates Neuropathic Pain by Inhibiting P2X7R Expression and ERK Phosphorylation in Rats

Jia-Piao Lin,Chao-Qin Chen,Ling-Er Huang,Na-na Li,Yan Yang,Sheng-Mei Zhu,Yong-Xing Yao 한국뇌신경과학회 2018 Experimental Neurobiology Vol.27 No.4

α2-Adrenoceptor agonists attenuate hypersensitivity under neuropathic conditions. However, the mechanisms underlying this attenuation remain largely unknown. In the present study, we explored the potential roles of purinergic receptor 7 (P2X7R)/extracellular signal-regulated kinase (ERK) signaling in the anti-nociceptive effect of dexmedetomidine in a rat model of neuropathic pain induced by chronic constriction injury (CCI) of the sciatic nerve. An animal model of CCI was adopted to mimic the clinical neuropathic pain state. Behavioral hypersensitivity to mechanical and thermal stimuli was determined by von Frey filament and Hargreaves’ tests, and the spinal P2X7R expression level and ERK phosphorylation were analyzed using western blot analysis and immunohistochemistry. In parallel with the development of mechanical and thermal hyperalgesia, a significant increase in P2X7R expression was noted in the ipsilateral spinal cord on day 7 after CCI. Intrathecal administration of dexmedetomidine (2.5 μg) for 3 days not only attenuated neuropathic pain but also inhibited the CCI-induced P2X7R upregulation and ERK phosphorylation. Intrathecal dexmedetomidine administration did not produce obvious effects on locomotor function. The present study demonstrated that dexmedetomidine attenuates the neuropathic pain induced by CCI of the sciatic nerve in rats by inhibiting spinal P2X7R expression and ERK phosphorylation, indicating the potential therapeutic implications of dexmedetomidine administration for the treatment of neuropathic pain.

Three Dimensional Nylon Cushioning Composite Fabrics: Manufacturing Technique and Property Evaluations

Jia-Ci Jhang,Ting Ru Lin,Yu-Chun Chuang,Ching-Wen Lou,Jia-Horng Lin 한국섬유공학회 2020 Fibers and polymers Vol.21 No.3

It is trendy to use polymers with different forms in a diversity of fields. This study uses nylon fibers and lowmelting-point polyester (LMPET) fibers or low melting point polylactic acid (LMPLA) fibers to fabricate three-dimensionalnylon/LMPET and nylon/LMPLA cushioning composite fabrics employing the nonwoven manufacturing. The employmentof needle punching process and thermal treatment reinforce the cushioning composite fabrics, and the subsequent thermalbonding points strengthen the mechanical properties effectively. In comparison to pure nylon nonwoven fabrics, the nyloncushioning composite fabrics exhibit higher tensile strength by 2.3 times regardless of whether it is a nylon/LMPET or nylon/LMPLA cushioning composite fabric. Similarly, based on the hammer rebound rate measurement, when the 3D cushioningcomposite fabrics are composed of 20 wt% of LMPLA fibers or 80 wt% of LMPET fibers, the hammer rebound rate reaches20 %, which is 1.4 times greater than that of the control group. Additionally, the composite fabrics that are composed of80 wt% of LMPLA fibers or 40 wt% of LMPET fibers also demonstrate higher compression recovery than the control group.

Experimental study on component performance in steel plate shear wall with self-centering braces

Jia-Lin Liu,Long-He Xu,Zhong-Xian Li 국제구조공학회 2020 Steel and Composite Structures, An International J Vol.37 No.3

Steel plate shear wall with self-centering energy dissipation braces (SPSW-SCEDB) is a lateral force-resisting system that exhibits flag-shaped hysteretic responses, which consists of two pre-pressed spring self-centering energy dissipation (PS-SCED) braces and a wall plate connected to horizontal boundary elements only. The present study conducted a series of cyclic tests to study the hysteretic performances of braces in SPSW-SCEDB and the effects of braces on the overall hysteretic characteristics of this system. The SPSW-SCEDB with PS-SCED braces only exhibits excellent self-centering capability and the energy loss caused by the large inclination angle of PS-SCED braces can be compensated by appropriately increasing the friction force. Under the combined effect of the two components, the SPSW-SCEDB exhibits a flag-shaped hysteretic response with large lateral resistance, good energy dissipation and self-centering capabilities. In addition, the wall plate is the primary energy dissipation component and the PS-SCED braces provide supplementary energy dissipation for system. The PS-SCED braces can provide up to 90% self-centering capability for the SPSW-SCEDB system. The compressive bearing capacity of the wall plate should be smaller than the horizontal remaining restoring force of the braces to achieve better self-centering effect of the system.

Relationship between Fractional Exhaled Nitric Oxide Level and Efficacy of Inhaled Corticosteroid in Asthma-COPD Overlap Syndrome Patients with Different Disease Severity

Jia-Xi Feng,Yun Lin,Jian Lin,Su-Su He,Mei-Fang Chen,Xiao-Mai Wu,You-Zu Xu 대한의학회 2017 Journal of Korean medical science Vol.32 No.3

This study explored the relationship between the fractional exhaled nitric oxide (FeNO) level and the efficacy of inhaled corticosteroid (ICS) in asthma-chronic obstructive pulmonary disease (COPD) overlap syndrome (ACOS) patients with different disease severity. A total of 127 ACOS patients with ACOS (case group) and 131 healthy people (control group) were enrolled in this study. Based on the severity of COPD, the ACOS patients were divided into: mild ACOS; moderate ACOS; severe ACOS; and extremely severe ACOS groups. We compared FeNO levels, pulmonary function parameters including percentage of forced expiratory volume in 1 second (FEV1) to predicted value (FEV1%pred), ratio of FEV1 to forced vital capacity (FEV1/FVC), inspiratory capacity to total lung capacity (IC/TLC) and residual volume to total lung capacity (RV/TLC), arterial blood gas parameters, including PH, arterial partial pressure of oxygen (PaO2) and arterial partial pressure of carbon dioxide (PaCO2), total serum immunoglobulin E (IgE), induced sputum eosinophil (EOS), plasma surfactant protein A (SP-A), plasma soluble receptor for advanced glycation end products (sRAGE), sputum myeloperoxidase (MPO), sputum neutrophil gelatinase-associated lipocalin (NGAL) and Asthma Control Test (ACT) scores, and COPD Assessment Test (CAT) scores. Compared with pre-treatment parameters, the FeNO levels, RV/TLC, PaCO2, total serum IgE, induced sputum EOS, plasma SP-A, sputum MPO, sputum NGAL, and CAT scores were significantly decreased after 6 months of ICS treatment, while FEV1%pred, FEV1/FVC, IC/TLC, PH, PaO2, plasma sRAGE, and ACT scores were significantly increased in ACOS patients with different disease severity after 6 months of ICS treatment. This finding suggests that the FeNO level may accurately predict the efficacy of ICS in the treatment of ACOS patients.

The multidirectional vibration and coupling dynamics of drill string and its influence on the wellbore trajectory

Lin Yang,Jia-lin Tian,Qingyou Liu,Liming Dai,Zhichao Hu,Jurui Li 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.7

For improving the accuracy of wellbore trajectory prediction in complex wellbore structures, especially in the kick-off section, a longitudinal, torsional and radial coupled drill string dynamics model is proposed. At the same time, the coupling dynamics model is solved based on the energy method, and the torque, weight on bit (WOB), the longitudinal displacement and velocity, the torsional angular displacement and angular velocity of the drill bit are obtained during the normal drilling and the stick-slip vibration phase. Combining the numerical example and experimental test, the differences between different models and actual drilling trajectories are compared and analyzed. The research results indicate that the torsional vibration and the longitudinal vibration have a great influence on each other in the coupling model, and the longitudinal vibration displacement increases, which can effectively reduce the torque variation range of the torsional vibration. The operation of the bit stagnation is weakened, maintaining a relatively stable rotational angular velocity of rotation. The calculation results of the wellbore trajectory are more consistent with the experimental results, and can accurately reflect the real situation of the drilling process. The theoretical model has an important reference for predicting the new well structure, especially the judgment of wellbore trajectory of complex wellbore structures such as extended reach wells, horizontal wells, deep wells and high deep wells of coalbed methane, and the prediction of downhole dynamics results.

Research and experimental analysis of drill string dynamics characteristics and stick-slip reduction mechanism

Jia-lin Tian,Lai Wei,Lin Yang,Liming Dai,Tangjia Zhang,He Liu 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.3

Stick-slip of the drill string, as one of the critical factors affecting drilling efficiency, has always been a hot topic for experts and scholars in related fields. Serious stickslip vibration affects the well construction efficiency, drilling cost and even lead to a downhole accident. Therefore, based on the current research, this paper takes a new type of composite vibration tool as the research object, studies the working mechanism of the tool and analyzes the effect of stick-slip reduction. After establishing the multi-degree-of-freedom torsional dynamics model, we obtained the results of torsional vibration dynamics by cases analysis based on working conditions and related mechanical parameters. In addition, the results of the examples, compared with the field experimental data, verify that the vibration shock generated by the new composite tool can effectively reduce the torque fluctuation range of the system, avoid the occurrence of stick-slip phenomenon, improve the ROP (rate of penetration), and make drilling process more stable. This paper can provide reference for the optimization and development of stick-slip technology in drilling engineering, and the theoretical methods can also be developed to study the dynamics of drill string.

Pressure-induced semiconductor-to-metal phase transition of a charge-ordered indium halide perovskite

Lin, Jia,Chen, Hong,Gao, Yang,Cai, Yao,Jin, Jianbo,Etman, Ahmed S.,Kang, Joohoon,Lei, Teng,Lin, Zhenni,Folgueras, Maria C.,Quan, Li Na,Kong, Qiao,Sherburne, Matthew,Asta, Mark,Sun, Junliang,Toney, Mic National Academy of Sciences 2019 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.116 No.47

<P><B>Significance</B></P><P>Metal halide perovskites attract great interest for a wide range of applications due to their remarkable optoelectronic properties. The development of environmentally friendly halide perovskite materials with various crystal structures and compositions offers unprecedented opportunities to achieve desired properties and applications. In this work, we demonstrated an In-based, charge-ordered all-inorganic halide double perovskite with the composition of Cs<SUB>2</SUB>In(I)In(III)Cl<SUB>6</SUB> synthesized by solid-state reaction. High-pressure optical properties were studied, and a pressure-driven, fully reversible semiconductor–metal phase transition was discovered. This In-based charge-ordered structure may inspire new understanding of halide perovskite as well as provide a platform for future discovery of exotic electronic phenomena such as high-<I>T</I><SUB>C</SUB> superconductivity in halide perovskite compounds.</P><P>Phase transitions in halide perovskites triggered by external stimuli generate significantly different material properties, providing a great opportunity for broad applications. Here, we demonstrate an In-based, charge-ordered (In<SUP>+</SUP>/In<SUP>3+</SUP>) inorganic halide perovskite with the composition of Cs<SUB>2</SUB>In(I)In(III)Cl<SUB>6</SUB> in which a pressure-driven semiconductor-to-metal phase transition exists. The single crystals, synthesized via a solid-state reaction method, crystallize in a distorted perovskite structure with space group <I>I</I>4/<I>m</I> with <I>a</I> = 17.2604(12) Å, <I>c</I> = 11.0113(16) Å if both the strong reflections and superstructures are considered. The supercell was further confirmed by rotation electron diffraction measurement. The pressure-induced semiconductor-to-metal phase transition was demonstrated by high-pressure Raman and absorbance spectroscopies and was consistent with theoretical modeling. This type of charge-ordered inorganic halide perovskite with a pressure-induced semiconductor-to-metal phase transition may inspire a range of potential applications.</P>