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RISS 인기검색어
- 검색키워드
- 저자 : Xiang-Shun Cui (검색결과 190 건)
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趙成九,崔香順,朴相一 충북대학교 농업과학기술연구소 2000 農業科學硏究 Vol.17 No.-
Diets containing 0.0%, 0.2%, 0.5%, and 1.0% of SLPBFL were fed to broiler chickens at 7 weeks age. The results of SLPBFL feeding are as follows; Body weight gain was significantly improved in 0.5% SLPBFL diet group ( p < 0.01 ). Because feed consumption was increased with adding of SLPBFL, broilers taste was not considered in this experiment. Feed requirement was analyzed same score to 1.99 in 0.2% and 0.5% SLPBFL added rations be improved about 8% than the control group. The amounts of carcass, drumsticks and breast meat were significantly(p<0.05) plenty in 0.5% treatment, and also the carcass ratio was enhanced to the highest score in 0.5% SLPBFL, In 0.5% treatment, birds were improved of growth rate, the head and neck weights, leg and shank weight, and amounts of blood and feather were higher appeared than other treatments. The weight of internal organs (liver, heart, spleen, gizzard) were appeared to be heavy in 0.5% treatment. The fat accumulation (abdominal and gizzard surrounding) was observed from the broiler chickens fed 0.5%, tended to increase with fat contents agreeably to live weight gain (P<0.05). Total serum protein, serum bilirubin and LDL-cholesterol concentrations in non- SLPBFL were lower than those in SLPBFL treatments. The concentrations of serum GPT, serum GOT, serum albumin and HDL- cholesterol in non-SLPBFL bird were the lowest in treatment. The concentrations of serum HDL-cholesterol and albumin in 0.5% treatment to be were appeared to be highest score and the concentration HDL-cholesterol was analyzed the lowest score, and total serum protein, serum GPT, serum GOT, serum bilirubin, total serum cholesterol, HDL-cholesterol and serum triglyceride concentrations were analyzed to be mean score in experimental diets. Key words : Bupleurum falcatum Linne, serum protein, serum GPT, serum GOT, serum Bilirubin, total serum cholesterol, HDL-cholesterol, serum triglyceride.
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趙成九,崔香順,朴相一 충북대학교 농업과학기술연구소 2000 農業科學硏究 Vol.17 No.-
Studies on the utilization added in diet with a Stem and Leaf Powder of Angelicae Gigas Nakai(SLPAGN) in the broiler chickens. Diets added with 0.0%, 0.2%, 0.5%, and 1.0% of SLPAGN were fed to broiler chickens at 7 weeks age. The results of SLPAGN feeding are as follows; The body weight gain was significantly improved in 1.0% SLPAGN diet (P<0.01), and feed efficiency was effected with 1.0% SLPAGN ration(P<0.05). The amount of carcass was the heaviest in 0.2% SLPAGN diet. The amounts of breast meat and drumsticks product were the highest in 0.2% SLPAGN, and the weights of head and neck, leg and shank were lower than other treatments. The fat accumulation (abdominal and gizzard surrounding) was observed in broilers fed diets added with 0.2% SLPAGN. The weights of liver, spleen and gizzard were tended to increase with adding SLPAGN rations than control diet. The degradation of fabricius sac was delayed to non-SLPAGN treatment than SLPAGN groups. The blood contents were analyzed to be the highest level to the total serum, GOT, total serum cholesterol and LDL-cholesterol concentrations in the broiler chickens fed 1.0% SLPAGN diets, and then the level of total serum protein and HDL-cholesterol concentrations were appeared to be the lowest. In the birds fed non-SLPAGN treatments the concentration of serum GPT, serum albumin, total serum triglyceride, total serum cholesterol and LDL-cholesterol were analyzed to be the lowest levels. In 0.2% SLPAGN treatment, the contents of serum GOT and serum bilirubin contents were analyzed the lowest levels. Key words: Angelicae Gigas, feed efficiency. HDL-cholesterol. Carcass ratio, serum bilirubin, GPT, serum albumin, serum triglyceride, serum albumin, GOT
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Cui, Xiang-Shun,Li, Xing-Yu,Shen, Xing-Hui,Bae, Yong-Ju,Kang, Jason-Jongho,Kim, Nam-Hyung JOHN WILEY & SONS LTD 2007 MOLECULAR REPRODUCTION AND DEVELOPMENT Vol.74 No.2
<P>To gain insight into early embryo development, we utilized microarray technology to compare gene expression profiles in four-cell (4C), morula (MO), and blastocyst (BL) stage embryos. Differences in spot intensities were normalized, and grouped by using Avadis Prophetic software platform (version 3.3, Strand Genomics Ltd.) and categories were based on the PANTHER and gene ontology (GO) classification system. This technique identified 622 of 7,927 genes as being more highly expressed in MO when compared to 4C (P < 0.05); similarly, we identified 654 of 9,299 genes as being more highly expressed in BL than in MO (P < 0.05). Upregulation of genes for cytoskeletal, cell adhesion, and cell junction proteins were identified in the MO as compared to the 4C stage embryos, this means they could be involved in the cell compaction necessary for the development to the MO. Genes thought to be involved in ion channels, membrane traffic, transfer/carrier proteins, and lipid metabolism were also identified as being expressed at a higher level in the BL stage embryos than in the MO. Real-time RT-PCR was performed to confirm differential expression of selected genes. The identification of the genes being expressed in here will provide insight into the complex gene regulatory networks effecting compaction and blastocoel formation. Mol. Reprod. Dev. © 2006 Wiley-Liss, Inc.</P>
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Cdc42 is implicated in polarity during meiotic resumption and blastocyst formation in the mouse
Cui, Xiang-Shun,Li, Xing-Yu,Kim, Nam-Hyung JOHN WILEY & SONS LTD 2007 MOLECULAR REPRODUCTION AND DEVELOPMENT Vol.74 No.6
<P>Cell division cycle 42 (Cdc42), a member of the Rho family of small guanosine triphosphatase (GTPase) proteins, regulates multiple cell functions, including: motility, proliferation, apoptosis, and cell morphology. In order to obtain insight into the role of Cdc42 in meiotic resumption and embryo development, we first evaluated its gene expression levels in mouse oocytes and embryos during in vitro development. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) revealed high-expression levels in GV stage oocytes that steadily decreased up to the 2-cell (2C) stage embryo, and then expression increased during morulae and blastocyst formation. Indirect Immunocytochemistry also showed protein synthesis of CDC42 in the mouse oocytes and early embryos. Introducing small interference RNA (siRNA) of Cdc42 into germinal vesicle stage oocytes or zygotes specifically reduced both mRNA expression and protein synthesis of CDC42 in in vitro developed metaphase II oocytes and early embryos. Meiotic maturation and cytoskeleton assembly were significantly altered following siRNA injection into germinal vesicle stage oocytes. Injection of siRNA into the zygote did not affect cleavage or cell numbers in morulae, but significantly decreased in vitro development to the morula or blastocyst. These findings suggest that gene expression of Cdc42 is involved in meiotic resumption and blastocyst formation in the mouse, possibly through maintaining polarity. Mol. Reprod. Dev. 74: 785–794, 2007. © 2006 Wiley-Liss, Inc.</P>
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Cui, Xiang-Shun,Lee, Jae Yeong,Choi, Seok Hwa,Kwon, Mo Sun,Kim, Teoan,Kim, Nam-Hyung 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8
The aim of this study was to determine the effects of mouse granulocyte-macrophage colony-stimulating factor (mGM-CSF) on development of porcine parthenotes and nuclear transferred embryos, and on their expression of implantation-related genes. In the presence of bovine serum albumin, mGM-CSF did not increase the percentage of oocytes that developed to the blastocyst stage and at day 7 did not increase cell numbers of embryos. Addition of 2 ng/ml GM-CSF to protein-free culture medium significantly increased the compaction and blastocoel formation of 1-to 2-cell parthenotes developing in vitro. However, total cell numbers were not increased when they were cultured in the presence of GM-CSF. Semi-quantitative reverse transcriptase polymerase chain reaction revealed that mGM-CSF enhances mRNA expression of the leukemia inhibitory factor receptor, but does not influence interleukin-6 or sodium/glucose co-transporter protein gene expression in blastocyst state parthenotes. These results suggest that mGM-CSF may enhance viability of porcine embryos developing in vitro in a defined culture medium.
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Cui, Xiang-Shun,Shen, Xing-Hui,Sun, Shao-Chen,Cho, Sun-Wha,Heo, Young-Tae,Kang, Yong-Kook,Wakayama, Teruhiko,Kim, Nam-Hyung Elsevier 2013 Journal of genetics and genomics Vol.40 No.4
<P>MicroRNAs (miRNAs) are a class of highly conserved small non-coding RNA molecules that play a pivotal role in several cellular functions. In this study, miRNA and messenger RNA (mRNA) profiles were examined by Illumina microarray in mouse embryonic stem cells (ESCs) derived from parthenogenetic, androgenetic, and fertilized blastocysts. The global analysis of miRNA-mRNA target pairs provided insight into the role of miRNAs in gene expression. Results showed that a total of 125 miRNAs and 2394 mRNAs were differentially expressed between androgenetic ESCs (aESCs) and fertilized ESCs (fESCs), a total of 42 miRNAs and 87 mRNAs were differentially expressed between parthenogenetic ESCs (pESCs) and fESCs, and a total of 99 miRNAs and 1788 mRNAs were differentially expressed between aESCs and pESCs. In addition, a total of 575, 5 and 376 miRNA-mRNA target pairs were observed in aESCs vs. fESCs, pESCs vs. fESCs, and aESCs vs. pESCs, respectively. Furthermore, 15 known imprinted genes and 16 putative uniparentally expressed miRNAs with high expression levels were confirmed by both microarray and real-time RT-PCR. Finally, transfection of miRNA inhibitors was performed to validate the regulatory relationship between putative maternally expressed miRNAs and target mRNAs. Inhibition of miR-880 increased the expression of Peg3, Dyrk1b, and Prrg2 mRNA, inhibition of miR-363 increased the expression of Nfat5 and Soat1 mRNA, and inhibition of miR-883b-5p increased Nfat5, Tacstd2, and Ppapdc1 mRNA. These results warrant a functional study to fully understand the underlying regulation of genomic imprinting in early embryo development.</P>
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CUI, Xiang-Shun,LI, Xing-Yu,YIN, Xi-Jun,KONG, IL Keun,KANG, Jason-Jongho,KIM, Nam-Hyung 家畜繁殖硏究所 2007 Journal of Reproduction and Development Vol.53 No.2
<P>Maternal gene expression is an important biological process in oocyte maturation and early cleavage. To gain insights into oocyte maturation and early embryo development, we used microarray analysis to compare the gene expression profiles of germinal vesicle (GV)- and metaphase II (MII)-stage oocytes. The differences in spot intensities were normalized and grouped using the Avadis Prophetic software platform. Of the 12164 genes examined, we found 1682 genes with more highly expression in GV-stage oocytes than in MII-stage oocytes, while 1936 genes were more highly expressed in MII-stage oocytes (P<0.05). The genes were grouped on the basis of the Panther classification system according to their involvement in particular biological processes. The genes that were up-regulated in GV oocytes were more likely to be involved in protein metabolism and modification, the mitotic cell cycle, electron transport, or fertilization or belong to the microtubule/cytoskeletal protein family. The genes specifically upregulated in the MII oocytes were more likely to be involved in DNA replication, amino acid metabolism, or expression of G protein-coupled receptors and signaling molecules. Identification of genes that are preferentially expressed at particular oocyte maturation stages provides insights into the complex gene regulatory networks that drive oocyte maturation and fertilization.</P>
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Cui, Xiang-Shun,Shin, Mi-Ra,Lee, Kyung-Ah,Kim, Nam-Hyung Wiley Subscription Services, Inc., A Wiley Company 2005 Molecular reproduction and development Vol.70 No.3
<P>The identification of embryo-specific genes would provide insights into early embryonic development. However, the current methods employed to identify the genes that are expressed at a specific developmental stage are labor intensive and suffer from high rates of false positives. Here we employed a new and accurate reverse transcription-polymerase chain reaction (RT-PCR) method that involves annealing control primers (ACPs) to identify the genes that are specifically or prominently expressed in mouse blastocysts compared to 4-cell stage embryos. Using 120 ACPs, we identified and sequenced 74 of these differentially expressed genes (DEGs). Basic Local Alignment Search Tool (BLAST) searches revealed that 53 were known genes, 9 encoded ribosomal proteins, and 12 were unknown genes. Of the known genes, 14 were selected and further characterized using real-time quantitative PCR to assess their stage-specific expression in mouse embryos. This analysis suggests that the ACP system is a very good method for the identification of stage-specific genes in small numbers of mouse embryos. Further analysis of the differentially expressed blastocyst genes we have identified will provide insights into the molecular basis of preimplantation development. Mol. Reprod. Dev. 70: 278–287, 2005. © 2005 Wiley-Liss, Inc.</P>
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