안도 다다오의 이우환 미술관의 개념적 관계성과 공간구성 분석

황인애(Hwang, In-Ae),이현수(Lee, Hyun-Soo) 한국실내디자인학회 2019 한국실내디자인학회 학술대회논문집 Vol.21 No.1

The abandoned island Naoshima was reborn with the will of Chairman Soichiro Fukudake and the efforts of Tadao Ando. Tadao Ando designed the Benesse House Museum, the Chichu Art Museum, and the Lee Ufan Museum, which are called the three major museums of Naoshima. The museum of Ando designed buildings that harmonize with architecture, nature, and human beings. Among them, Lee Ufan of Lee Ufan Museum is a painter who focused on relationality in particular. In this study, the Lee Ufan Museum was selected as an example. This research focuses on the analysis of their conceptual relationship with the Lee Ufan Museum and the spatial composition that it implemented in architecture. The research methods include literature review, drawing analysis and field trip. Lee"s work trends can be seen to be concentrated on the relationship such as ‘objects-objects’, ‘objects-spaces’, and ‘objects-human beings’. From the perspective of Lee Ufan"s conceptual relationships, the architecture of Tadao Ando can be understood as the relationship such as ‘architecture-exposed concrete(matter)’, ‘architecture-human(moving line)’, and ‘architecture-nature(place)’. Through this analysis, the Lee Ufan Museum is not merely a solo work of Tadao Ando, but a successful case in which two outstanding artists to keep in step together as a collaboration between Ando and Lee.

오차드그래스(Dactylis glomerata L.)의 품종에 따른 종자유래의 캘러스 형성률과, 캘러스 크기 및 식물체 재분화 효율의 비교

배은경,이인애,김기용,이병현,손대영,이효신,정민섭,조진기 Plant molecular biology and biotechnology research 2002 Plant molecular biology and biotechnology research Vol.2002 No.-

Comparison results of callus formation ratio from seed explants, callus sizes, regeneration ratios from callus and regeneration efficiency [calculated by following formular; callus formation ratio(%) × regeneration ratio(%)/100]for 27 ochardgrass (Dactylis glomerata L.)varieties imported and Hapsung 2 developed in Korea are as follows; 1. Among ochardgrass varieties showing more than 50% callus formation ratios, the descending order of callus formation ratio after bedding the seed explants for 4 weeks was 93>Sparta>Pizza>Condor>Lidaglo>Glorus>Hapsung2>Frode. 2. The callus sizes after bedding for 4 weeks were in the range of φ 0.43cm~4.2cm in which there was 10 times size difference between the largest one and the smallest one but most of them were between φ2.5cm~4cm. 3. The regeneration ratio from callus among varieties were in the range of 0~36% and descending order of the upper 6 varieties was Plano>Akimidori>Justus>Lidacta>Currie>Hall mark. 4. The regeneration efficiency which is calculated by the ratios of regeneration from seed explant numbers was between 0 to 17.4% among which Justus showed the highest value in the 4-week treatment. 5. The correlation between callus formation ratios and the callus sizes, callus formation ratios and regeneration efficiency, and callus sizes and regeneration efficiency were r=0.5765, r=0.6365 and r=0.6246, respectively in 4-week callus and all the correlations were significant on the 1% level. 6. In 6-week callus, the descending order callus formation ratios from seed sxplants for the best 6 varieties was Condor>Sparta>93M>Justus>Potomac>Lidaglo>Frode. 7. The callus sizes formed were between φ1.5~5.7cm in which Sparta, the largest one of φ5.7cm was five times larger than the smallest one. The callus size of the control variety. Hapsung 2 was φ3.8cm, which belonged to larger size. 8. Regeneration ratio showed a great deviation among varieties from 6-week old calli by showing from 0% to 100% in which all the calli were regenerated in Plano while no callus was regenerated in Juno, 9. The range of regeneration effciency was between 0~28% among varieties in which the values from 6-week callus treatment were larger than those from 4-week callus treatment. Especially, the value of Potomac in 6-week was 3 times larger than that in 4-week. 10. The correlation between callus formation ratios and the callus sizes, callus formation ratios and regeneration efficiency, and callus sizes and regeneration effciency were r=0.8369, r=0.6683 and r=0.5937, respectively in 6-week callus, and all the correlations were significant on the 1% level.

Quantitative Proteomic Analysis Identifies AHNAK (Neuroblast Differentiation-associated Protein AHNAK) as a Novel Candidate Biomarker for Bladder Urothelial Carcinoma Diagnosis by Liquid-based Cytology

Lee, Hyebin,Kim, Kwangsoo,Woo, Jongmin,Park, Joonho,Kim, Hyeyoon,Lee, Kyung Eun,Kim, Hyeyeon,Kim, Youngsoo,Moon, Kyung Chul,Kim, Ji Young,Park, In Ae,Shim, Bo Bae,Moon, Ji Hye,Han, Dohyun,Ryu, Han Suk American Society for Biochemistry and Molecular Bi 2018 Molecular and Cellular Proteomics Vol.17 No.9

<P>Cytological examination of urine is the most widely used noninvasive pathologic screen for bladder urothelial carcinoma (BLCA); however, inadequate diagnostic accuracy remains a major challenge. We performed mass spectrometry-based proteomic analysis of urine samples of ten patients with BLCA and ten paired patients with benign urothelial lesion (BUL) to identify ancillary proteomic markers for use in liquid-based cytology (LBC). A total of 4,839 proteins were identified and 112 proteins were confirmed as expressed at significantly different levels between the two groups. We also performed an independent proteomic profiling of tumor tissue samples where we identified 7,916 proteins of which 758 were differentially expressed. Cross-platform comparisons of these data with comparative mRNA expression profiles from The Cancer Genome Atlas identified four putative candidate proteins, AHNAK, EPPK1, MYH14 and OLFM4. To determine their immunocytochemical expression levels in LBC, we examined protein expression data from The Human Protein Atlas and in-house FFPE samples. We further investigated the expression of the four candidate proteins in urine cytology samples from two independent validation cohorts. These analyses revealed AHNAK as a unique intracellular protein differing in immunohistochemical expression and subcellular localization between tumor and non-tumor cells. In conclusion, this study identified a new biomarker, AHNAK, applicable to discrimination between BLCA and BUL by LBC. To our knowledge, the present study provides the first identification of a clinical biomarker for LBC based on in-depth proteomics.</P>

臨床에서의 看護過程 適用에 對한 實態調査 : 慶北地域 看護專門大學 3學年 學生 및 이들 實習病院의 看護員을 對象으로

金仁淑,劉愛光,李淑姬 김천과학대학 1980 김천과학대학 논문집 Vol.8 No.-

The purpose of our research was to find the importance and application of the nursing process to actual clinical situations and to find the difference between practical experience and theoretical study. We are researched this by using the questionnaire process. We are questioned 420 nurse college students in six nurse's colleges in the Kyung Buk region. The following is the conclusion and classification of our research. 1. The majority of those questioned were between the ages of 21 and 29-that is, 90.26% of the 420 senior nursing students and 78.3% of the nurses now working in college -related hospitals. 2. In the case of on-site clinical practice, our research reflected that those who practiced the nursing process "occasionally" in such cases numbered highest at 69.08% while those who practiced the process "many times" numbered an extremely low 14.01%.(p<0.05) 3. Our research also reflected that 36.59% of those who had carefully studied each subject while studying the nursing process(10.2% of the total number questioned) responded that they applied their study "many times" in on-site clinical situations. (p<0.01) 4. As to the importance of the nursing process, 80.5% of the students and 83.53% of the nursing responded very positively. Though there is only a small difference between the responses of the students and the nurses, still this difference does carry some meaning. 5, Only 7.58% of the student group and 14.01% of the surses responded that they applied the nursing process "many times" but 61.12% of the students and 69.08% of the surses responded that it was applied "occasionally." Again, the difference between nurses and students carries meaning. (p<0.01) 6. Our research showed that 31.65% of the surses responded that they taught the nursing process to student nurses, but only 17.71% of the student nurses responded that had been taught nursing practice by hospital nurses. Similarly, 13.9% of nurses responded that they had been taught nursing practice by hospital nurses. Similarly, 13.9% of nurses responded that they did not teach nursing practice to student nurses, while 67% of the student nurses said they were not instructed at all by hospital nurses. Our research showed, therefore, that there is a great difference of opinion between the students and the regular nurses. (p<0.01) 7. Of the clinical hospital nurses questioned 60.15% responded that they teach student nurses by experience without continued instruction in the regular course of nursing. Also, 48.83% indicated that they give limited assignments to student nurses, deviating from the normal assigment method of instruction. This indicates a difference in the understanding of making specific clinical aims. (p<0.01) 8. As to student clinical practice, 65.01% of the nurses answered that they knew the purpose of it, but 22.01% answered that they did not know the, purpose of it.

특수체육교육학과 학생들의 교원임용시험 준비 경험에 관한 근거이론적 분석

이동철 ( Dong-chul Lee ),김인애 ( In-ae Kim ) 한국특수체육학회 2019 한국특수체육학회지 Vol.27 No.2

본 연구는 근거이론 방법을 바탕으로 특수체육교육학과 학생들의 교원임용시험 준비과정을 탐색하여 그들의 경험을 심층적으로 이해하는데 목적이 있다. 연구 목적 달성을 위해 유목적 표집방법(purposeful sampling)으로 교원임용시험 준비 경험이 2년 이상 있는 특수체육교육학과 졸업생 8명을 연구 참여자로 선정하였다. 자료 수집은 개방형 설문을 이용한 심층 면담을 통해 이루어졌으며 자료 분석은 Strauss와 Colbin(1996)이 제시한 근거이론 분석방법에 따라 개념을 도출하고 개념 간의 관계 과정을 규명하기 위해 코딩 패러다임을 구성하였다. 분석결과를 토대로 다음과 같은 결론을 얻었다. 연구 참여자들의 임용시험 준비과정에서 중심현상은 시험 준비과정에서의 난관으로 도출되었다. 이러한 중심현상을 초래하는 인과적 상황은 불확실한 미래에 대해 심리적 갈등이었으며, 이에 대한 맥락적 상황으로 교원 임용시험제도에 대한 부담과 경제적 부담감, 환경적인 어려움을 겪고 있었다. 또한 연구 참여자들은 중요타자의 지지, 환경적 지원, 긍정적 경험 등의 중재적 조건을 통해 임용시험 준비과정에 어려움을 극복하기 위해 노력하고 있었으며, 행동/상호작용 전략으로는 긍정적 사고의 전환, 철저한 자기관리, 적극적 실천 노력으로 나타났다. 결국 연구 참여자들은 임용시험 준비 과정에서 난관에 직면하였을 때 임용시험에 합격하여 자신의 꿈인 교사가 꼭 될 수 있다는 자기 확신을 갖고 이를 극복하기 위한 적극적인 노력을 하고 있었다. This study investigated the experiences of graduates majoring in adapted physical education during the preparation process for secondary teacher employment examination based on the grounded theory method. In order to achieve the purpose of this study, eight graduates of adapted physical education with more than two years of experience in preparation for teacher employment examination were selected as the study participants by purposeful sampling. Data collection was conducted through in-depth interviews using open-ended questionnaires. Data analysis derived concepts and consisted of coding paradigms to identify the process of relationship between concepts through the grounded theory analysis method suggested by Strauss and Colbin(1998). The result was as follows. The central phenomenon in the preparation for examination of participants was derived from the difficulty of preparation for the examination. The reason of the central phenomenon was the psychological conflict about the uncertain future, and the contextual situation for this was the burden on examination system, financial status, and personal environment. In addition, participants were trying to overcome difficulties in process of examination through interventional conditions such as support of significant others, environmental support, positive experience. Behavior and interaction strategies consisted of transition of positive thinking, self-management, and active practice. As a result, when participants were confronted with difficulties in preparation of examination, they tried to overcome with self-confidence that they will be able to become teachers.

ERK1/2-Mediated Schwann Cell Proliferation in the Regenerating Sciatic Nerve by Treadmill Training

Seo, Tae Beom,Oh, Myung-Jin,You, Byoung-Gun,Kwon, Ku-Birm,Chang, In-Ae,Yoon, Jin-Hwan,Lee, Chan-Yong,Namgung, Uk Mary Ann Liebert 2009 JOURNAL OF NEUROTRAUMA - Vol.26 No.10

<P>Proliferation of Schwann cells in the injured peripheral nerve supports axonal regeneration, and physical training in experimental animals has been shown to promote nerve regeneration. Extracellular signal-regulated kinase 1/2 (ERK1/2) activity can mediate neuronal responses to lesion signals, but its role in non-neuronal cells in the injured area is largely unknown. Here we report that treadmill training (TMT) facilitates axonal regeneration via the upregulation of phospho-ERK1/2 protein levels in Schwann cells in the injured sciatic nerve. Low-intensity, but not high-intensity, TMT increased neurite outgrowth of dorsal root ganglion (DRG) sensory neurons and potentiated Schwann cell proliferation. TMT elevated levels of GAP-43 mRNA and protein, and phospho-ERK1/2 protein in the injured sciatic nerves. TMT also enhanced phospho-c-Jun protein levels in the injured nerve. In-vivo administration of the ERK1/2 inhibitor PD98059 eliminated phospho-c-Jun, suggesting ERK1/2 phosphorylation of the c-Jun protein. PD98059 treatment decreased levels of BrdU-labeled proliferating Schwann cells in the distal portion of the injured nerve, and delayed the axonal regrowth that was promoted by TMT. The present data suggest that increased ERK1/2 activity in Schwann cells may play an important role in TMT-mediated enhancement of axonal regeneration in the injured peripheral nerve.</P>

보건소를 이용하는 지역주민의 건강 행위와 건강관련 체력간의 관계

전미양,최명애,이인숙,김태수,김의숙,박선홍 대한보건협회 2002 대한보건연구 Vol.28 No.3

The purpose of this study is to identify relationship health behavior and health related physical fitness in order to provide the basic data for health promotion program for community people. The study was conducted 299 people, during the period from June 1, 2000 to October 30, 2000. The result are as follows. 1. Female comprised was 63.2%(189), while male comprised 36.8%(110). The age range of the subject were from 21 to 59 years old. The subject were aged 30s group 52.5%, 40s group 29.1%, 50s group 12.7%, 20s group 5.7%. Twenty subjects (6.7%) had experience smoking and one subject (0.3%) in female and nineteen subjects (6.4%) in male. Seventy-five subjects (25.1%) had experience drinking and twenty-nine subjects (9.7%) in female, and forty-six (15.4%) in male subjects. ninety-nine subjects had exercise and sixty subjects (20.1%) in female subjects and thirty-mine subject (13%) in male. 2. Body fat and flexibility were higher in female than male subjects, but cardioresperatory endurance, muscular strength and muscular endurance were higher in male than female subjects. 3. There were significant differences in body fat and muscular endurance between age group in both gender subjects, and in cardioresperatory endurance and muscular strength between the age group in male subjects. 4. No significant differences health related fitness between non-smoking and smoking group in both gender subjects. 5. No significant differences health related fitness between non-alcohol and alcohol group in both gender subjects. 6. For female subjects, muscular strength, muscular endurance and flexibility of the exercise group were significantly higher than those of the non exercise group. for male subjects, cardiorespiratory endurance of the exercise group were significantly higher than those of the non exercise group.

Validation of Sea Surface Temperature (SST) from Satellite Passive Microwave Sensor (GPM/GMI) and Causes of SST Errors in the Northwest Pacific

Kim, Hee-Young,Park, Kyung-Ae,Chung, Sung-Rae,Baek, Seon-Kyun,Lee, Byung-Il,Shin, In-Chul,Chung, Chu-Yong,Kim, Jae-Gwan,Jung, Won-Chan The Korean Society of Remote Sensing 2018 大韓遠隔探査學會誌 Vol.34 No.1

Passive microwave sea surface temperatures (SST) were validated in the Northwest Pacific using a total of 102,294 collocated matchup data between Global Precipitation Measurement (GPM) / GPM Microwave Sensor(GMI) data and oceanic in-situ temperature measurements from March 2014 to December 2016. A root-mean-square (RMS) error and a bias error of the GMI SST measurements were evaluated to $0.93^{\circ}C$ and $0.05^{\circ}C$, respectively. The SST differences between GMI and in-situ measurements were caused by various factors such as wind speed, columnar atmospheric water vapor, land contamination near coastline or islands. The GMI SSTs were found to be higher than the in-situ temperature measurements at low wind speed (<6 m/s) during the daytime. As the wind speed increased at night, SST errors showed positive bias. In addition, other factors, coming from atmospheric water vapor, sensitivity degradation at a low temperature range, and land contamination, also contributed to the errors. One of remarkable characteristics of the errors was their latitudinal dependence with large errors at high latitudes above $30^{\circ}N$. Seasonal characteristics revealed that the errors were most frequently observed in winter with a significant positive deviation. This implies that SST errors tend to be large under conditions of high wind speeds and low SSTs. Understanding of microwave SST errors in this study is anticipated to compensate less temporal capability of Infrared SSTs and to contribute to increase a satellite observation rate with time, especially in SST composite process.

국내육성 오차드그라스 품종들의 캘러스 형성율 및 식물체 재분화 효율

김기용,강경민,배은경,이인애,임용우,최기준,박근제,손대영,조진기 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Comparisons of callus formation ratios from seed explants, callus sizes, regeneration ratios from callus and regeneration efficiency for 4 orchardgrass (Dactylis glomerata L.) varieties (Three were developed in Korea and one was imported from foreign nation) are as follows; Jangbeol 102 (67.0%) has the highest callus formation ratio in 4 weeks incubated callus after bedding the seed explants, but Potomac (68.4%) has the highest ratio in 6-seek callus. Potomac (3.93cm) has the highest callus size in 4-week callus, but Jangbeol 101 (4.32cm) has the highest size in 6-week callus. Jangbeol 101 (17.7%) has the highest plant regeneration ratio in 4-week callus, but Potomac (37.4%) has the highest raion in 6-week callus. Jangbeol 102 (11.5%) has the highest plant regeneration efficiency in 4-week callus, but Potomac (25.6%) has the highest efficiency in 6-week callus.

과산화수소를 이용한 현장원위치 화학적 산화법과 공기분사법(Air-sparging)을 연계한 디젤 오염 토양/지하수 동시 정화 실내 실험 연구

김남호,김인수,최애정,이민희,Kim, Nam-Ho,Kim, In-Su,Choi, Ae-Jung,Lee, Min-Hee 한국지하수토양환경학회 2006 지하수토양환경 Vol.11 No.6

현장비원위치(Ex-situ) 공법 적용이 불가능한 부지에서, 디젤로 오염된 오염 토양과 지하수를 동시에 복원하기 위하여 과산화수소를 이용한 현장원위치 화학적 산화법(chemical oxidation)과 공기분사법(air-sparging)을 연계한 복합 복원 공정의 정화 효율 규명을 위한 실내 실험을 실시하였다. TPH 농도가 2,401 mg/kg(A 토양)과 9,551 mg/kg(B 토양)인 두 종류의 현장 오염 토양을 대상으로 과산화수소용액을 이용한 화학적 산화법의 디젤 제거 효율 규명을 위한 배치(회분식) 실험 결과, 과산화수소 50% 용액에 의해 토양 초기 TPH 농도의 18%와 15%까지 감소하였다. 과산화수소 용액 20%를 이용한 칼럼 세정 실험 결과, 세정에 의해서 A 토양과 B 토양의 경우 각각 초기 TPH 양의 78%와 72%가 제거되었다. 칼럼 실험에서 과산화수소의 산화반응에 의해 완전 분해되어 무기가스상(주로 $CO_2$과 $H_2O$)으로 제거된 양까지 고려한다면, 과산화수소용액이 오염 토양과 접촉하면서 충분한 산화과정을 거쳐 대부분의 유류가 토양으로부터 제거되었음을 알 수 있었다. 공기분사법을 이용한 디젤 오염 지하수 정화 실험의 경우, TPH 농도가 820 mg/L인 고농도의 인공 지하수 경우에도 공기분사 72시간 이내에 폐수배출허용기준인 5 mg/L 보다 낮아져, 디젤 제거 효율이 매우 높은 것으로 나타났다. 다만, 오염 토양 내 다량의 디젤 자유상이 존재하는 경우 토양으로부터 지하수로의 지속적인 자유상 디젤의 질량 이동에 의하여, 공기분사법의 지하수 정화 효율은 매우 낮았다. 마지막으로, 과산화수소를 이용한 현장원위치 화학적 산화법과 공기분사법을 연계한 복합 공정의 디젤 정화 효율을 규명하는 박스 실험을 실시하였다. 토양 내 자유상 디젤을 먼저 제거하기 위해 과산화수소 용액을 이용한 토양세정법을 실시한 후, 토양 내 TPH가 제거 되는 정도에 따라 후차적으로 공기분사법을 적용함으로써 토양 및 지하수로부터 디젤을 효과적으로 제거할 수 있었다. 20% 과산화수소 용액의 23 L 세정과 2,160 L의 공기분사에 의해 토양의 TPH 농도는 9,551 mg/kg에서 390 mg/kg으로 낮아졌으며, 오염 지하수의 TPH 농도도 5 mg/L 이하로 낮출 수 있었다. 본 실험들에서 얻어진 결과를 바탕으로 실제 현장에서 대단위 공정을 운영하는데 필요한 복원 공정의 최적 조건들을 도출해 낼 수 있으리라 판단되며, 유류로 오염된 토양 뿐 아니라 오염 지하수까지 동시에 정화할 수 있는 복합 공정 개발을 위한 중요한 기술 자료로 이용될 수 있을 것으로 판단된다. Laboratory scale experiments were performed to investigate the removal efficiency of the in-situ chemical oxidation method and the air-sparging method for diesel contaminated soil and groundwater. Two kinds of diesel contaminated soils (TPH concentration : 2,401 mg/kg and 9,551 mg/kg) and groundwater sampled at Busan railroad station were used for the experiments. For batch experiments of chemical oxidation by using 50% hydrogen peroxide solution, TPH concentration of soil decreased to 18% and 15% of initial TPH concentration. For continuous column experiments, more than 70% of initial TPH in soil was removed by using soil flushing with 20% hydrogen peroxide solution, suggesting that most of diesel in soil reacted with hydrogen peroxide and degraded into $CO_2$ or $H_2O$ gases. Batch experiment for the air-sparging method with artificially contaminated groundwater (TPH concentration : 810 mg/L) was performed to evaluate the removal efficiency of the air-sparging method and TPH concentration of groundwater decreased to lower than 5 mg/L (waste water discharge tolerance limit) within 72 hours of air-sparging. For box experiment with diesel contaminated real soil and groundwater, the removal efficiency of air-sparging was very low because of the residual diesel phase existed in soil medium, suggesting that the air-sparging method should be applied to remediate groundwater after the free phase of diesel in soil medium was removed. For the last time, the in-situ box experiment for a unit process mixed the chemical oxidation process with the air-sparging process was performed to remove diesel from soil and groundwater at a time. Soil flushing with 20% hydrogen peroxide solution was applied to diesel contaminated soils in box, and subsequently contaminated groundwater was purified by the air-sparging method. With 23 L of 20% hydrogen peroxide solution and 2,160 L of air-sparging, TPH concentration of soil decreased from 9,551 mg/kg to 390 mg/kg and TPH concentration of groundwater reduced to lower than 5 mg/L. Results suggested that the combination process of the in-situ hydrogen peroxide flushing and the air-sparging has a great possibility to simultaneously remediate fuel contaminated soil and groundwater.