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Proton Transfer Hydrogels: Versatility and Applications
Hwang, JiHyeon,Lee, Dong G.,Yeo, Hyunki,Rao, Jingyi,Zhu, Zhiyuan,Shin, Jawon,Jeong, Keunsoo,Kim, Sehoon,Jung, Hyun Wook,Khan, Anzar American Chemical Society 2018 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.140 No.21
<P>Proton transfer polymerization between thiol and epoxide groups is shown to be an adaptable and utilitarian method for the synthesis of hydrogels. For instance, the polymerization catalyst can be organic or inorganic, and the polymerization medium can be pure water, buffer solutions, or organic solvents. The gelation mechanism can be triggered at ambient conditions, at a physiological temperature of 37 °C, or through using light as an external stimulus. The ambient and photochemical methods both allow for nanoimprint lithography to produce freestanding patterned thick films. The required thiol- and epoxide-carrying precursors can be chosen from a long list of commercially available small molecular as well as polymeric materials. The water uptake, mechanical, and biodegradation properties of the gels can, therefore, be tuned through the choice of appropriate gelation precursors and polymerization conditions. Finally, the thio-ether groups of the cross-linked networks can be functionalized through a postgelation modification reaction to access sulfonium-based cationic structures. Such structural changes endow antibacterial properties to the networks. In their pristine form, however, the gels are biocompatible and nonadhesive, allowing cancer cells to grow in a cluster formation.</P> [FIG OMISSION]</BR>
Web-based design and analysis tools for CRISPR base editing
Hwang, Gue-Ho,Park, Jeongbin,Lim, Kayeong,Kim, Sunghyun,Yu, Jihyeon,Yu, Eunchong,Kim, Sang-Tae,Eils, Roland,Kim, Jin-Soo,Bae, Sangsu BioMed Central 2018 BMC bioinformatics Vol.19 No.1
<P><B>Background</B></P><P>As a result of its simplicity and high efficiency, the CRISPR-Cas system has been widely used as a genome editing tool. Recently, CRISPR base editors, which consist of deactivated Cas9 (dCas9) or Cas9 nickase (nCas9) linked with a cytidine or a guanine deaminase, have been developed. Base editing tools will be very useful for gene correction because they can produce highly specific DNA substitutions without the introduction of any donor DNA, but dedicated web-based tools to facilitate the use of such tools have not yet been developed.</P><P><B>Results</B></P><P>We present two web tools for base editors, named BE-Designer and BE-Analyzer. BE-Designer provides all possible base editor target sequences in a given input DNA sequence with useful information including potential off-target sites. BE-Analyzer, a tool for assessing base editing outcomes from next generation sequencing (NGS) data, provides information about mutations in a table and interactive graphs. Furthermore, because the tool runs client-side, large amounts of targeted deep sequencing data (< 1 GB) do not need to be uploaded to a server, substantially reducing running time and increasing data security. BE-Designer and BE-Analyzer can be freely accessed at http://www.rgenome.net/be-designer/ and http://www.rgenome.net/be-analyzer/, respectively.</P><P><B>Conclusion</B></P><P>We develop two useful web tools to design target sequence (BE-Designer) and to analyze NGS data from experimental results (BE-Analyzer) for CRISPR base editors.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (10.1186/s12859-018-2585-4) contains supplementary material, which is available to authorized users.</P>
Comparison of Machine Learning Models According to Data Trends for Predicting Tomato Growth
Hyunjung Hwang,Hyuntae Lee,Seungwoo Cha,Jihyeon Choi,Hwanyong Choi,Sangjun Chung,Seoheui Lee,Junhyuck Jang,Christopher RETITI DIOP EMANE,Yuna Kim,Jongtae Lim,Kyoungsoo Bok,Jaesoo Yoo 한국콘텐츠학회 2022 한국콘텐츠학회 ICCC 논문집 Vol.2022 No.12