Genetic Toxicity Test of Nanosilica by Comet assay and Mouse Lymphoma Assay

Youn?Jung Kim(김연정),Han Seam Choi(최한샘),Dong Ge Ra Mi Moon(문동그라미),Ji Hee Kim(김지희),Hye Gyeong Jo(조혜경),Jae Chun Ryu(류재천) 환경독성보건학회 2007 한국독성학회 심포지움 및 학술발표회 Vol.- No.-

콘크리트 보수용 폴리머 시멘트 모르타르의 적정 배합비 및 두께 결정

이윤수,이기원,전철수,지경용 강원대학교 부설 석재복합신소재 제품연구센터 2000 석재연 논문집 Vol.5 No.-

피로하중, 산성비, 염해 및 동결융해 등 각종 원인에 의해 시멘트 콘크리트 구조물의 성능 저하가 건설산업분야의 문제점으로 대두되고 있다. 일반적으로 성능이 저하된 시멘트 콘크리트 구조물의 보수재로는 폴리머 복합재료가 널리 사용되고 있다. 보통 시멘트 콘크리트에 폴리머를 첨가하면, 작업성의 경우 폴리머의 혼입량이 증가 할수록 작업성이 증가하고 이에 따라 공기량이 증가하는 경향을 나타내었다. 또한 압축강도는 폴리머 시멘트비가 10%에서 가장 높은 값을 보였으며, 휨강도의 경우는 폴리머 시멘트비가 증가할수록 강도가 증가하는 경향을 나타내었다. 또한 폴리머 시멘트 모르타르의 직접인장접착강도에서는 폴리머-시멘트비 10%, 소포제 1%에서 가장 높은 접착강도가 나타났으며, 내후성 시럼 후의 직접인장접착강도 실험에서는 보두 수께 5mm가 가장 높은 접착강도를 나타내었다. 이상과 같은 실험 결과를 볼 때 보수재로 사용되는 폴리머 시멘트 모르타르의 경우는 폴리머 시멘트비 10%, 소포제1%의 배합으로 약 5mm의 두께로 보수를 하는 것이 가장 유리함을 알 수 있었다. This research was conducted to experimentally evaluate the characteristics of the tensile adhesion strength of polymer cement mortar for repair of cement concrete structures. Air content of polymer cement mortar was improved with increment of polymer cement ratio. Water absorption of polymer cement mortar was decreased with increment of polymer cement ratio. Compressive strength of polymer cement ratio 10% was higher than those of others. Flexural strength of polymer cement mortar was improved with increment of polymer cement ration. The tensile adhesion strength of polymer cement ration 10% and antiformer agent content 1% was higher than those of others. After weathering test, the tensile adhesion strength of thickness 5mm was found to be higher than those of others.

한국형아유르베다 심리유형을 통한 경영자 사례분석

김지은(Kim, Ji-eun),윤천성(Youn, Chun-Sung) 한국평생교육리더십학회 2020 평생교육리더십연구 Vol.7 No.1

본 연구는 코로나19 바이러스 위기 상황에서 경영자의 경영능력 이 위기 상황을 극복하는 가장 중요한 능력이라고 판단하고 한국형아유르베다 심리유형을 통한 경영자 사례분석을 하였다. 문헌으로는 윤천성(2017)의 국내에서 발간한 한국형아유르베다 교재를 토대로 하였고, 학위논문은 학술연구정보서비스(RISS)에서 아유르베다 검색한 키워드 109개의 논문 중 ‘한국형아유르베다’에 관련된 연구를 참조하였으며, 2018년 이후 기준으로 석·박사학위논문과 학술지에 대하여 고찰하였다. 연구의 목적은 무한 경쟁사회 및 경제시장에서 위기를 대처할 기업경영에 필요한 이론 중 현재 경영자들에게 유용하다고 판단되는 한국형아유르베다 심리유형 을 통한 경영자 사례분석을 통해 경영자들에게 안정감을 주는 한국형아유르베다 균형유형 시사점을 도출하고자 한다. 본 연구의 한계점과 이를 발전시킬 향후 연구방향은 첫째, 한국형아유르베다 심리유형에 대한 국내외 선행연구가 부족하여 선행연구를 통해 개념화하기 부족하므로, 한국형아유르베다 심리유형 검사의 연구와 기존에 많이 이루어졌던 심리유형 검사를 비교분석하는연 구가 더 활발하게 진행되어야 할 것이다. 둘째, 기존의 경영자 연구를 한국형아유르베다 심리유형 검사를 투입하여 각 유형이 가지는 장점과 특성을 바탕으로 연구한다면 다양한 연구 사례를 만들 것으로 판단된다. This study judged that the management ability of managers in the crisis situation of the Corona 19 virus was the most important ability to overcome the crisis situation, and analyzed the case of managers through the Korean Ayurveda psychological pattern. The literature was based on the Korean Ayurveda textbook published in Korea by Chun-Sung Yun (2017), and the thesis is a study related to the Korean Ayurveda among 109 papers with keywords searched for Ayurveda by the Academic Research Information Service (RISS), And reviewed master s and doctoral dissertations and academic journals as of 2018. The purpose of the study is to provide a sense of stability to managers through the analysis of manager cases through the Korean Ayurveda psychology type that is considered useful to current managers among the theories necessary for corporate management to cope with crises in a competitive society and economic market. This is to derive the implications of Ayurveda Balance Type . The limitations that can be brought about as a result of this study and the future research direction to develop it are: First, there is a lack of domestic and foreign prior studies on Korean Ayurvedic psychological types, so it is insufficient to conceptualize through prior studies. It is necessary to conduct a more active study comparing and analyzing the psychological pattern tests that have been conducted a lot in the past. Second, if the existing managerial research is studied based on the strengths and characteristics of each type by introducing the Korean Ayurveda psychological type test, it is believed that various research cases will be made.

효제문자도 병풍의 장황

전지연(CHUN Ji-youn) 한국민화학회 2019 한국민화 Vol.- No.10

필자는 조선후기부터 근 · 현대기의 병풍 그림을 어떤 형태의 장황(粧䌙)으로 보존처리해야 할 것인가에 관한 질문을 필두로 연구를 시작하였고, 보존처리를 직접 수행하고 있는 연구자들에게 그 방향을 제시하는 것이 이번 연구의 목적이다. 우선 그림에 나타난 병풍과 실제 유물을 통해 조선 병풍의 시대별 장황 변천을 소개하였고, 현대기에 들어 잘못된 수리 및 재표구로 인한 개장(改粧)의 문제점들에 대해서도 알아보았다. 다음으로 ‘효제문자도’를 중심으로 지역별 병풍 장황 양식을 제시하였다. 일제 후기부터는 교통의 발달로 지역 구분이 무의미할 수도 있지만, 선연구된 효제문자도의 지역 작가 계보를 바탕으로 도상과 장황을 연계, 분류하여 지역별 병풍 장황 특성을 알아보았다. 강원 지역의 효제문자도 병풍에서는 작가가 그림을 그리고 직접 장황까지 마무리한 ‘석강 황승규’라는 작가를 주목해 볼 필요가 있다. 황승규의 효제문자도 병풍을 보면, 병풍에 사용된 장황 재료, 색상, 비례 등에서 작가만의 특유한 장황 양식이 존재함을 알 수 있다. 강원 지역의 효제문자도는 문자도에 화조, 책거리, 산수 등을 조합하는 형식으로, 다른 지역 효제문자도 병풍에 비해 병풍이 높은 특징을 보이며, 높이는 약 140~150㎝의 중형 병풍이다. 제주 지역의 효자문자도 병풍은 높이 약 100~110㎝로 낮다. 대부분이 그림 자체만을 틀에 붙여 만든 것으로, 육지의 문자도 병풍에 비해 제작방법이 간단하며, 병풍 다리가 높은 특징이 있다. 샤를 바라가 밀양에서 구입한 병풍 그림과 도상학적으로 유사한 문자도는 이번 연구에서 경상 지역 병풍으로 분류하였다. 이 병풍들은 높이 약 100~120㎝의 비교적 낮은 병풍으로, 조선 민간 병풍 양식을 이어가고 있으며, 효제문자도가 뒷면에 배치된 경우도 발견된다. 이번 연구에서는 ‘효제문자도’ 병풍을 중심으로만 장황 양식을 살펴보았는데, 추후 다른 주제에 대해서도 마찬가지로 다룰 계획이다. This study started at the need of finding the right form of mounting to restore folding screen paintings produced since the late Chosun dynasty. I intend to present the right direction to conservators in the field how they should restore art works in these years. First, I gave an overview on mounting changes in Korean folding screens by era and also indicated some important problems made by wrong methods of mounting in modern times. Next, I discovered several regional features of folding screen mountings from Hyoje Munjado(painting of characters for filial duty and brotherly love). My research is based on prior studies on the genealogy of regional folding screen artists. I studied regional features of folding screen mountings, interconnecting iconography, mounting and artist genealogy. We need to keep an eye on Seokang Hwang Seung-kyu in Kangwon folding screen Hyoje Munjado. He painted folding screen pictures and completed mounting work himself. We can find peculiar features on his folding screens such as materials, colors and proportions. Folding screens in Kangwon region have mixed forms of character paintings, flower-bird paintings, bookshelves paintings, and landscape paintings. They are higher than those in other regions with height of 140~150cm. Folding screens in Cheju region are low with the height of 100~110cm. They are mostly made by just pasting pictures on the folding screen frame and legs are higher compared with other regions. Folding screens in Kyungsang region are relatively low with the height of 100~120cm. They are keeping the traditional form of popular folding screen in Chosun times. In some cases Hyoje Munjado are placed on the back of the folding screen. This study focused only on the mounting forms of folding screen with Hyoje Munjado. Expanding the object of study to other popular art works since Chosun dynasty, there will be a bulk of new findings waiting to be discovered for a long time.

Myristoleic Acid Promotes Anagen Signaling by Autophagy through Activating Wnt/β-Catenin and ERK Pathways in Dermal Papilla Cells

( Youn Kyung Choi ),( Jung-il Kang ),( Jin Won Hyun ),( Young Sang Koh ),( Ji-hoon Kang ),( Chang-gu Hyun ),( Kyung-sup Yoon ),( Kwang Sik Lee ),( Chun Mong Lee ),( Tae Yang Kim ),( Eun-sook Yoo ),( H 한국응용약물학회 2021 Biomolecules & Therapeutics(구 응용약물학회지) Vol.29 No.2

Alopecia is a distressing condition caused by the dysregulation of anagen, catagen, and telogen in the hair cycle. Dermal papilla cells (DPCs) regulate the hair cycle and play important roles in hair growth and regeneration. Myristoleic acid (MA) increases Wnt reporter activity in DPCs. However, the action mechanisms of MA on the stimulation of anagen signaling in DPCs is not known. In this study, we evaluated the effects of MA on anagen-activating signaling pathways in DPCs. MA significantly increased DPC proliferation and stimulated the G2/M phase, accompanied by increasing cyclin A, Cdc2, and cyclin B1. To elucidate the mechanism by which MA promotes DPC proliferation, we evaluated the effect of MA on autophagy and intracellular pathways. MA induced autophagosome formation by decreasing the levels of the phospho-mammalian target of rapamycin (phospho-mTOR) and increasing autophagy-related 7 (Atg7) and microtubule-associated protein 1A/1B-light chain 3II (LC3II). MA also increased the phosphorylation levels of Wnt/β-catenin proteins, such as GSK3β (Ser⁹) and β-catenin (Ser⁵⁵² and Ser⁶⁷⁵). Treatment with XAV939, an inhibitor of the Wnt/β-catenin pathway, attenuated the MA-induced increase in β-catenin nuclear translocation. Moreover, XAV939 reduced MA-induced effects on cell cycle progression, autophagy, and DPC proliferation. On the other hand, MA increased the levels of phospho (Thr²⁰²/Tyr²⁰⁴)-extracellular signal regulated kinases (ERK). MA-induced ERK phosphorylation led to changes in the expression levels of Cdc2, Atg7 and LC3II, as well as DPC proliferation. Our results suggest that MA promotes anagen signaling via autophagy and cell cycle progression by activating the Wnt/β-catenin and ERK pathways in DPCs.

Bactericidal Efficacy of a Disinfectant Spray Containing a Grapefruit-seed Extract, Citric acid, Malic acid and Benzalkonium Chloride against Salmonella Typhimurium and Brucella ovis

Chun-Nam Cha,Eun-Kee Park,Ji-Youn Jung,Chang-Yeul Yoo,Suk Kim,Hu-Jang Lee 한국식품위생안전성학회 2016 한국식품위생안전성학회지 Vol.31 No.4

살모넬라증과 부루셀라증은 가축 및 사람에 심각한 피해를 유발하는 질병으로, 축산업과 식품산업에 많은 경제적 손실을 초래하고 있다. 본 연구에서는, 자몽종자추출물, 구연산, 사과산 그리고 염화벤잘코늄을 주성분으로 하는 스프레이형 소독제의 Salmonella Typhimurium과 Brucella ovis에 대한 효력시험을 수행하였다. 살균효력시험은 배지희석법에 따라 수행하였으며, 스프레이형 소독제와 시험 세균들을 처리조건에 따라 경수와 유기물로 희석하여 반응을 시켰다. 유기물 조건에서, Salmonella Typhimurium과 Brucella ovis에 대한 스프레이형 소독제의 살균력은 경수조건에서의 살균력과 비교하여 낮게 나타났는데, 이는 유기물들에 의한 소독제의 살균 유효성분에 대한 저해작용에 따른 것으로 사료된다. 스프레이형 소독제는 Salmonella Typhimurium과 Brucella ovis와 같은 인수공통전염병 유발 병원균들에 대해 살균효과를 나타내어, 살모넬라증과 브루셀라증과 같은 세균성 질병의 확산을 제어하는데 효과적으로 이용될 수 있을 것으로 사료된다. Salmonella spp. and Brucella spp. can cause considerable diseases on both humans and animals. In addition, these microorganisms cause the economic loss in animal farming and food industry. In this study, the disinfection efficacy of a disinfectant spray, composed to grapefruit seed extract, citric acid, malic acid and benzalkonium chloride, was evaluated against S. Typhimurium and B. ovis. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at 4℃. The disinfectant and test bacteria were diluted with hard water (HW) or organic matter suspension (OM) according to treatment condition. On HW condition, the bactericidal activity of the disinfectant spray against S. Typhimurium and B. ovis was 5 and 4 fold dilutions, respectively. On OM condition, the bactericidal activity of the disinfectant spray was 2 and 1 fold dilutions against S. Typhimurium and B. ovis, respectively. As the disinfectant spray possesses bactericidal efficacy against foodborne pathogens such as S. Typhimurium and B. ovis, the disinfectant spray can be used to control the spread of bacterial diseases.

Effect of intraperitoneal CO<SUB>2</SUB> concentration on postoperative pain after laparoscopic cholecystectomy

Ji Won Chung,Kyu Sik Kang,Sang Hyun Park,Chun Sook Kim,Jin Hun Chung,Sie Hyeon Yoo,Nan Seol Kim,Yong Han Seo,Ho Soon Jung,Hea Rim Chun,Hyung Youn Gong,Hae Il Jung,Sang Ho Bae,Su Yeon Park 대한외과학회 2017 Annals of Surgical Treatment and Research Vol.93 No.4

Purpose: This study set out to identify the association between the intraperitoneal CO2 concentrations and postoperative pain by dividing the participants into a control group and 2 experimental groups receiving irrigation (1 L and 2 L), and directly measuring their intraperitoneal CO2 concentrations with a CO2 gas detector. Methods: A total of 101 patients, American Society of Anesthesiologists physical status classification I and II patients aged 18–65 years were enrolled in the study. Group 1 did not receive irrigation with normal saline, while groups 2 and 3 were administered irrigation with 1 L and 2 L of normal saline, respectively, after laparoscopic cholecystectomy. Intraperitoneal CO2 concentrations were measured with a CO2 gas detector through the port, and postoperative pain was assessed on a visual analogue scale at 6, 12, and 24 hours after surgery. Results: The intraperitoneal CO2 concentrations were 1,016.0 ± 960.3 ppm in group 1, 524.5 ± 383.2 ppm in group 2, and 362.2 ± 293.6 ppm in group 3, showing significantly lower concentrations in groups 2 and 3. Postoperative pain was significantly lower in group 3 at 6 hours after surgery, and in groups 2 and 3 at 12 hours after the surgery. However, there was no significant difference between the 3 groups in postoperative pain 24 hours after the surgery. Conclusion: This study found a causal relationship between the amount of normal saline used for irrigation and the intraperitoneal CO2 concentrations in that irrigation with normal saline reduces pain on the day of the surgery.

Virucidal efficacy of a paraformaldehyde-containing fumigant against Avian influenza virus

( Chun Nam Cha ),( Eun Kee Park ),( Chang Yeul Yoo ),( Ji Youn Jung ),( Song Ee Son ),( Suk Kim ),( Hu Jang Lee ) 한국예방수의학회 2016 예방수의학회지 Vol.40 No.2

In this study, the virucidal efficacy of a fumigant (35% paraformaldehyde) against avian influenza virus (AIV) was examined. After AIV suspensions had been deposited on stainless steel carriers, the dried carriers were exposed to the fumigant in a 300-m3 test room for 3 h. Thereafter, all carriers were submerged in a neutralizing solution to scrape off the surviving viruses, and the respective suspensions were diluted. Each dilution factor was respectively inoculated into the allantoic membrane of five 10-day-old embryos. After incubation, AIV viability in the collected allantoic fluids was examined and the EID50 was calculated. The fumigant inactivated ≥5.7log10EID50 of AIV and was nontoxic to the embryos.

Differential interaction between Selenoprotein M and apoptosis-related proteins in HEK 293 cell and Transgenic rat overexpressing the Human Selenoprotein M

Ji Soon Sin,Yong Kyu Kim,Chul Kyu Kim,Byung Guk Kim,Sun Bo Shim,Seung Wan Jee,Su Hae Lee,Chang Jun Bae,Byung Chun Lee,Mi Kyong Jang,Min Sun Kim,Su Youn Yim,Insurk Jang,Jung sik Cho,Kab Ryong Chae,Dae 한국실험동물학회 2007 한국실험동물학회 학술발표대회 논문집 Vol.2007 No.-

Genetic Toxicity Test of Nanosilver by Comet assay and Mouse Lymphoma Assay

Youn-Jung Kim(김연정),Han-Seam Choi(최한샘),Dong-Ge-Ra-Mi Moon(문동그라미),Ji-Hee Kim(김지희),Hye-Gyeong Jo(조혜경),Jae-Chun Ryu(류재천) 환경독성보건학회 2007 한국독성학회 심포지움 및 학술발표회 Vol.- No.-