응급수술후 발생한 당뇨병성 케톤산증 및 고중성지방혈증으로 인한 급성췌장염 1예

목지오 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Severe hypertriglyceridemia in diabetic ketoacidosis are rarely observed in acute pancreatitis which usually occurred when serum triglyceride levels are above 1,000㎎/dl. Auther report a case of diabetic ketoacidosis and hypertriglyceridemia-induced acute pancreatitis after emergency operation. A 14 year old female was presented with right lower abdominal pain. She was diagnosed acute appendicitis and received appendectomy. After emergency operation, she showed polydipsia, dry mouth and drowsiness. Blood gas analysis showed severe acidosis and serum ketone body was positive. The laboratory finding showed serum glucose 412㎎/dl, HbA1C 16.9%, total cholesterol 586㎎/dl, triglyceride 1,333㎎/dl, amylase 1,942U/L, lipase 2,251IU/L. She was diagnosed as having diabeteic ketoacidosis and hypertriglyceridemia-induced acute pancreatitis. Findings of her abdominal CT was acute pancreatitis with formation of pseudocyst. She treated with continuous intravenous insulin infusion and fluid replacement without feeding. After conservative management of 15 days, general condition gradually improved and her serum glucose, amylase, lipase and triglyceride levels ware normalized. After 13weeks, findings of abdominal CT showed improved pancreatitis and pseudocyst.

갑상선 종양에서 thrombin receptor (PAR-1)의 발현 양상

박지영,목지오,김여주,박형규,김철희,변동원,서교일,유명희,조현득,오미혜,김상진 순천향의학연구소 2006 Journal of Soonchunhyang Medical Science Vol.12 No.2

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폐렴 구균(Streptococcus pneumoniae)에 의한 척수 경막외 농양 1예

이지영,위유미,손경목,기현균,문치숙,오원섭,백경란,송재훈 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

폐렴구균은 보통 폐렴이나 뇌막염을 일으키는 균으로 척수 경막외 농양을 일으키는 경우는 매우 드물다. 본 저자들은 폐렴 구균에 의한 척수 경막 외 농양을 경험하여 보고하는 바이다. 과거 건강했던 36세 남자 환자로 사다리에서 낙상한 후 요통 및 고열, 신경학적 이상으로 내원하여 자기 공명 촬영 결과 척수 경막 외 농양으로 진단받고 항균제 투여와 함께 응급 수술을 시행하였다. 농 배양 결과 페니실린 감수성인 폐렴 구균이 분리 되었고, 수술 요법과 항생제 투여 후 농양은 치유되었으나 신경학적 이상은 호전 없는 상태로 타원으로 전원되었다. Pneumonia and meningitis are the most frequent manifestations of pneumococcal infections. Pneumococcal spinal epidural abscesses have been rarely reported. Spinal epidural abscess by Streptococcus pneumoniae has been diagnosed among the patients with diabetes mellitus, alcoholism, corticosteroid therapy, intravenous drug use, chronic renal failure, AIDS, and history of spinal surgery. Recently, we experienced a case of pneumococcal spinal epidural abscess after spinal trauma. A 36-year-old male patient was admitted with back pain, fever, and paraplegia which occurred 5 days after the trauma. Spine MRI revealed spinal epidural abscess at the level from T2 to T9. He was treated with antimicrobial agents and surgical exploration for spinal epidural abscess. Pus culture grew S. pneumoniae which was susceptible to penicillin. Despite early surgical treatment, neurologic sequelae remained. Considering the high mortality and morbidity of pneumococcal spinal epidural abscess, early diagnosis and aggressive treatment including surgical intervention and antibiotics therapy should be implemented immediately.

관동맥 조영술상 관동맥의 심근교 현상에 관한연구

권영주,목지오 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

BACKGROUND: Myocardial bridging is defined as embedment of a segment of the coronary artery into the myocardium so that during ventricular systole the segment of artery is compressed. The prevalance of myocardial bridges among consecutive patients coronary angiography varies between 0.5% and 16% with most studies reporting an incidence of less than 2%. Because any studies is not reported about myocardial bridging in Korea, this study was done to establish the angiographic evidence of myocardial bridging in cineangiograms performed in the cardiac laboratory of the Soonchunhang University PATIENTS AND METHODS: In the 10-years period between 1987 and 1996, selective coronary angiography was performed on 1079 patients at the Medical University of Soonchunhang Hospitals. All studies were reviewed and patients were divided into three groups on the basis of the degree of systolic compression: Mild(1-30% systolic compression). Moderate(31-50% systolic compression), and Severe(51-100% systolic compression). RESULTS: 1. 10 PATIENTS(0.93%) Were found to have myocardial bridge. and 9 patients had systolic compression of the left anterior descending coronary artery and one patient had compression of the right coronary artery. 2. Cineangiograms of all 292 "normal" patients who had symptomes suggestive of angina pectoris were reviewed and 7 patients(2.4%) were found to have myocardial bridging. 3. 4 patients had mild myocardial bridge, 1 patient had moderate, and 5 patients had severe. 4. The ECG was normal in 4 patients, showed left ventricular hypertrophy in 5 patients, and showed atrial fibrillation. Treadmill exercise test was positive in 5 patients, and non-diadnostic test due to insufficient exercise capacity was 5 patients. CONCLUSIONS: The angiographic incidence of myocardial bridges was noted was noted 0.93% and most common site is the left anterior descending artery.

Endoscopic comparison of alendronate alone and the enteric-coated alendronate with calcitriol combination in postmenopausal Korean females

( Ji Oh Mok ),( Chan Hee Jung ),( Chul Hee Kim ),( Chang Beom Ryu ),( Yeo Joo Kim ),( Sang Jin Kim ),( Hyeong Kyu Park ),( Kyo Il Suh ),( Myung Hi Yoo ),( Dong Won Byun ) 대한내과학회 2013 The Korean Journal of Internal Medicine Vol.28 No.6

Background/Aims: This study was performed to compare the mucosal findings after esophagogastroduodenoscopy in two groups before and after the use of alendronate only and following administration of the enteric-coated alendronate (5 mg) and calcitriol (0.5μg) combined drug (Maxmarvil, Yuyu Co.). Methods: The study population consisted of 33 postmenopausal healthy female volunteers, aged 50 to 70 years (mean age, 58 ± 5) without gastrointestinal symptoms and with normal baseline endoscopic findings. Esophagogastroduodenoscopy was performed at baseline and was repeated 2 weeks later after daily intake of Maxmarvil (n = 17 subjects) or alendronate only (n = 16 subjects). Mucosal injury scores were reported by an endoscopist after 2 weeks of treatment with each medication schedule. Results: Esophageal mucosal injuries developed in two of 16 subjects in the alendronate only group and 0 of 17 in the Maxmarvil group. Gastric mucosal injuries developed in eight subjects in the alendronate group and four subjects in the Maxmarvil group; this difference was statistically significant. Conclusions: The mucosal damage scores for the alendronate group (total score 24) were significantly higher than those for the Maxmarvil group (total score 9) in the esophagus and stomach. Therefore, this study suggested that enteric-coated Maxmarvil is less harmful to gastrointestinal mucosa than alendronate, and may improve the tolerability of osteoporosis medication in clinical practice.

증례 : 급성 A형 간염 후 발생한 전격성 제1형 당뇨병 1예

권지연 ( Ji Yeon Kwon ),노미오 ( Mi Oh Roh ),송민수 ( Min Soo Song ),정찬희 ( Chan Hee Jung ),박형규 ( Hyeong Kyu Park ),김여주 ( Yeo Ju Kim ),목지오 ( Ji Oh Mok ),김상진 ( Sang Jin Kim ),김철희 ( Chul Hee Kim ),변동원 ( Dong Won 대한당뇨병학회 2009 임상당뇨병 Vol.10 No.2

전격성 제1형 당뇨병은 췌도에서 급격히 베타세포와 알파세포가 파괴되는 특징을 가진 당뇨병으로 그 기전은 명확하지 않으나 유전적 감수성이 있는 개체에서 바이러스 감염 등의 환경적인 인자와의 연관성이 가능한 병태생리로 제시되고 있으며 이에 대한 추가적인 연구가 필요하다. 저자들은 최근 급증하는 A형 간염 후에 발생한 전격성 제1형 당뇨병 1예를 경험하여 전격성 제1형 당뇨병과 바이러스 감염의 관련성에 대한 근거를 제시하고 이에 대해 보고하는 바이다. Fulminant type 1 diabetes mellitus is a novel subtype of type 1 diabetes, which is characterized by a remarkably abrupt onset, metabolic derangement such as diabetic ketoacidosis at diagnosis, low HbA1c level at onset and negative islet-related autoantibodies. The pathogenesis of this disease remains to be clarified, but the involvement of both genetic background and viruses have been suggested. We recently encountered a case of fulminant type 1 diabetes mellitus in 32-year-old male patient who was diagnosed with acute hepatitis A a month ago. Laboratory findings revealed high blood glucose level as well as evidence of diabetic ketoacidosis, but the HbA1c level was normal. The low level of plasma C-peptide indicated the loss of endogenous insulin secretion. This patient`s laboratory results met the criteria for the diagnosis of fulminant type 1 diabetes. We consider that this report can add to the body of evidence of a viral etiology of fulminant type 1 diabetes. Further study is needed to elucidate the exact pathogenesis. (Korean Clinical Diabetes J 10:118-122, 2009)

Gene Profiles in a Smoke-Induced COPD Mouse Lung Model Following Treatment with Mesenchy-mal Stem Cells

Yeon-Mok Oh,You-Sun Kim,Nurdan Kokturk,Ji-Young Kim,Sei Won Lee,Jaeyun Lim,Soo Jin Choi,Wonil Oh 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.10

Mesenchymal stem cells (MSCs) effectively reduce airway inflammation and regenerate the alveolus in cigarette- and elastase-induced chronic obstructive pulmonary disease (COPD) animal models. The effects of stem cells are thought to be paracrine and immune-modulatory because very few stem cells remain in the lung one day after their systemic injection, which has been demonstrated previously. In this report, we analyzed the gene expression profiles to compare mouse lungs with chronic exposure to cigarette smoke with non-exposed lungs. Gene expression profiling was also conducted in a mouse lung tissue with chronic exposure to cigarette smoke following the systemic injection of human cord blood-derived mesenchymal stem cells (hCB-MSCs). Globally, 834 genes were differentially expressed after systemic injection of hCB-MSCs. Seven and 21 genes, respectively, were up-and down-regulated on days 1, 4, and 14 after HCB-MSC injection. The Hbb and Hba, genes with oxygen transport and anti-oxidant functions, were increased on days 1 and 14. A serine protease inhibitor was also increased at a similar time point after injection of hCB-MSCs. Gene Ontology analysis indicated that the levels of genes related to immune responses, metabolic processes, and blood vessel development were altered, indicating host responses after hCB-MSC injection. These gene expression changes suggest that MSCs induce a regeneration mechanism against COPD induced by cigarette smoke. These analyses provide basic data for understanding the regeneration mechanisms promoted by hCB-MSCs in cigarette smoke-induced COPD.

A novel approch of usage of Gram staining to indentify Mycobacterium tuberculosis from tissues

Jin Mok Kim(Jin Mok Kim),Ji Eun Oh(Ji Eun Oh),Reye Kim(Reye Kim),Yeong-Jin Heo(Yeong-Jin Heo),Jae-Hyeon Cho(Jae-Hyeon Cho) 한국예방수의학회 2023 예방수의학회지 Vol.47 No.1

Tuberculosis is a potentially deadly infectious disease caused by the Mycobacterium tuberculosis (M. tuberculosis). Tuberculosis is diagnosed by proving the M. tuberculosis in sputum samples based on the results of acid-resistant staining, culture, and nucleic acid amplification tests. However, there is a report that the detection rate of M. tuberculosis is low in acid-resistant staining using tissue specimens. It has been suspected that the cause is a potential loss of acid resistance by the organic solvents used for tissue specimen preparation. Therefore, this study was pursued to find out if Gram staining and fluorescent staining in addition to acid-resistant staining would be helpful in diagnosing tuberculosis. We used four tissue (lung, small intestine, large intestine, and lymph node) samples with chronic granulomatous inflammation observed in HE staining and positive results in real-time PCR. These detection rates and staining properties were investigated through microscopic examination using the Ziehl-Neelsen, Gram, and Auramin rhodamine staining. In this studies, M. tuberculosis were observed by Ziehl-Neelsen, Gram, and Auramin rhodamine staining in all four samples. In the evaluation of clinical microbiology proficiency testing (CMPT), the Ziehl-Neelsen and Gram staining were the same result, but the Auramin rhodamine staining was relatively low. These data indicated that Gram staining is useful for detecting M. tuberculosis in formalin-fixed tissue specimens. Therefore, if the Ziehl-Neelsen and Gram staining are combined as the M. tuberculosis staining method in tissue specimens, a better direction may be provided for tuberculosis diagnosis.

증례 : 내분비-대사 ; 범뇌하수체저하증, 요붕증을 보인 원발성 육아종성 뇌하수체염 1예

오현진 ( Hyun Jin Oh ),목지영 ( Ji Young Mok ),김지은 ( Ji Eun Kim ),조성배 ( Sung Bae Cho ),장상아 ( Sang Ah Chang ),김지현 ( Ji Hyun Kim ),이정민 ( Jung Min Lee ) 대한내과학회 2015 대한내과학회지 Vol.88 No.5

본 증례에서는 기저 질환이 없던 48세 여자 환자가 두통, 피로함, 오심, 희발 월경, 다뇨 증상을 호소하였고 호르몬 검사와 영상 검사 결과 범뇌하수체 기능 저하증과 중심성 요붕증을 동반하는 뇌하수체 병변으로 진단하였다. 6개월간 스테로이드 및 호르몬 요법을 시행했으나 두통이 지속되고 종괴의 크기도 변하지 않아 접형동을 통한 종괴 절제술을 시행하였다. 수술 후 원발성 육아종성 뇌하수체염으로 확진할 수 있었으며 현재 호르몬 보충 요법을 유지하면서 외래에서 경과관찰 중이다. Primary granulomatous hypophysitis is a rare inflammatory disorder of the pituitary gland and patients commonly present with symptoms of sellar compression and hypopituitarism. A 48-year-old woman was admitted due to headache and fatigue. Magnetic resonance imaging showed a 21 × 18 × 13-mm round sellar mass with a thickened pituitary stalk. The endocrinological examination revealed panhypopituitarism and diabetes insipidus. Suspecting hypophysitis, the patient was given steroid and hormone replacement therapy. Six months later, she continued to complain of severe headaches and nausea. Computed tomography showed no significant change in the sellar mass. Subsequently, transsphenoidal surgery was performed. The pathological examination revealed granulomatous changes with multinucleated giant cells and primary granulomatous hypophysitis was diagnosed. Her headache resolved, but the pituitary functions did not improve. This is the first reported case in Korea of primary granulomatous hypophysitis with dysfunction of anterior and posterior pituitary gland, including the stalk, without optic chiasm compression. (Korean J Med 2015;88:581-586)

Characterization of a Korean Domestic Cyanobacterium Limnothrix sp. KNUA012 for Biofuel Feedstock

Ji Won Hong(홍지원),Seung-Woo Jo(조승우),Oh Hong Kim(김오홍),Mi Rang Jeong(정미랑),Hyeon Kim(김현),Kyung Mok Park(박경목),Kyoung In Lee(이경인),Ho-Sung Yoon(윤호성) 한국생명과학회 2016 생명과학회지 Vol.26 No.4

사상체 토착 남세균을 경상남도 합천군 합천호의 수화시료로부터 무균적으로 분리하였으며, 형태적 · 분자적 동정 결과 림노트릭스 속에 속하는 것으로 밝혀졌다. 따라서, 본 남세균 균주는 림노트릭스 속 KNUA012 균주로 명명하였으며, 분리균주의 최적생장 온도는 섭씨 25도였다. 지질성분 분석 결과, 에스테르 교환반응을 거치지 않고 직접 연료로 사용할 수 있는 펜타데칸(C15H32)과 헵타데칸(C17H36)과 같은 알칸들이 본 균주에 의해 광독립 영양적으로 생합성 된다는 것이 밝혀졌다. 또한 알칸 생합성에 관여하는 유전자들이 본 남세균 내에 존재하는 것을 발견하였다. 일반적인 미세조류 바이오디젤 구성성분으로 알려진 미리스트올레산(C14:1), 팔미트산(C16:0) 및 팔미톨레산(C16:1) 역시 KNUA012 균주에 의해 주요 지방산 성분으로서 생산되는 것으로 확인되었다. 근사분석 결과 KNUA012 균주의 휘발성물질 함량은 86.0%였으며, 원소분석 결과 고위발열량은 19.8 MJ kg<SUP>-1</SUP>으로 나타났다. 또한, 본 분리균주는 고부가가치 항산화물질로 알려져 있는 피코시아닌을 광독립영양적으로 21.4 mg g<SUP>-1</SUP>의 농도로 생산할 수 있는 것을 확인하였다. 따라서, 본 연구결과는 KNUA012 균주가 미세조류 기반 바이오연료와 바이오매스 원료의 경제적인 생산에 있어 이상적인 자원이 될 수 있음을 제시하였다. A filamentous cyanobacterium, Limnothrix sp. KNUA012, was axenically isolated from a freshwater bloom sample in Lake Hapcheon, Hapcheon-gun, Gyeongsangnam-do, Korea. Its morphological and molecular characteristics led to identification of the isolate as a member of the genus Limnothrix. Maximal growth was attained when the culture was incubated at 25°C. Analysis of its lipid composition revealed that strain KNUA012 could autotrophically synthesize alkanes, such as pentadecane (C15H32) and heptadecane (C17H36), which can be directly used as fuel without requiring a transesterification step. Two genes involved in alkane biosynthesis-an acyl-acyl carrier protein reductase and an aldehyde decarbonylase-were present in this cyanobacterium. Some common algal biodiesel constituents-myristoleic acid (C14:1), palmitic acid (C16:0), and palmitoleic acid (C16:1)-were produced by strain KNUA012 as its major fatty acids. A proximate analysis showed that the volatile matter content was 86.0% and an ultimate analysis indicated that the higher heating value was 19.8 MJ kg<SUP>-1</SUP>. The isolate also autotrophically produced 21.4 mg g<SUP>-1</SUP> phycocyanin-a high-value antioxidant compound. Therefore, Limnothrix sp. KNUA012 appears to show promise for application in cost-effective production of microalga-based biofuels and biomass feedstock over crop plants.