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Yeon-Mok Oh,You-Sun Kim,Nurdan Kokturk,Ji-Young Kim,Sei Won Lee,Jaeyun Lim,Soo Jin Choi,Wonil Oh 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.10
Mesenchymal stem cells (MSCs) effectively reduce airway inflammation and regenerate the alveolus in cigarette- and elastase-induced chronic obstructive pulmonary disease (COPD) animal models. The effects of stem cells are thought to be paracrine and immune-modulatory because very few stem cells remain in the lung one day after their systemic injection, which has been demonstrated previously. In this report, we analyzed the gene expression profiles to compare mouse lungs with chronic exposure to cigarette smoke with non-exposed lungs. Gene expression profiling was also conducted in a mouse lung tissue with chronic exposure to cigarette smoke following the systemic injection of human cord blood-derived mesenchymal stem cells (hCB-MSCs). Globally, 834 genes were differentially expressed after systemic injection of hCB-MSCs. Seven and 21 genes, respectively, were up-and down-regulated on days 1, 4, and 14 after HCB-MSC injection. The Hbb and Hba, genes with oxygen transport and anti-oxidant functions, were increased on days 1 and 14. A serine protease inhibitor was also increased at a similar time point after injection of hCB-MSCs. Gene Ontology analysis indicated that the levels of genes related to immune responses, metabolic processes, and blood vessel development were altered, indicating host responses after hCB-MSC injection. These gene expression changes suggest that MSCs induce a regeneration mechanism against COPD induced by cigarette smoke. These analyses provide basic data for understanding the regeneration mechanisms promoted by hCB-MSCs in cigarette smoke-induced COPD.
( Wonil Park ),( Kwang Min Kim ),( Hyun Chin Cho ),( Ji Eun Oh ),( Chang Uk Jeong ),( Dae Hyeon Cho ),( Kil Jong Yu ),( Man Je Park ),( Hyoun Soo Lee ),( Sang Goon Shim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Although there have been many attempts to improve the patency rate of the biliary stents in patients with unresectable perihilar cholangiocarcinoma, Controversy remains over how best to improve the patency rate of the biliary stents. We assessed the clinical outcomes of the placement of triple metal stents in patients undergoing palliative biliary drainage for perihilar cholangiocarcinoma. Methods: After deployment of the fi rst stent into the common bile duct, the two other stents were sequentially introduced at the hilar confi uence via bilateral percutaneous transhepatic biliary drainage catheters. A total of 11 consecutive patients with unresectable perihilar cholangiocarcinoma of Bismuth type III or higher who underwent percutaneous placement of self-expandable, uncovered triple metal stents were evaluated retrospectively. Results: Technical success was achieved in all cases, demonstrated by contrast medium. During the follow-up period, successful drainage, which was defi ned as decrease in bilirubin to <75% of the pre-treatment value within the fi rst months, was obtained in 10 of 11 patients (90. 9%). Two patients who developed acute cholangitis after stent placement were treated using antibiotics only, with no additional intervention, whereas one patient who developed acute cholecystitis required re-intervention. There was no procedure-related or 30 day mortality. None of these patients underwent additional metallic stent placement, secondary to recurrent cholangitis or stent occlusion within 30 days. Conclusions: Our results show that placement of triple metal stents in patients with unresectable perihilar cholangiocarcinoma could be an effective and safe alternative to improve the rate of successful biliary decompression and stent patency.
Changhyun Kim,Wonil Chang,Sang-Hoon Oh,Soo-Young Lee IEEE Signal Processing Society 2014 IEEE signal processing letters Vol. No.
<P>With the increasing importance of smart gadgets in our daily lives, there is a need for an automatic piano transcription system in various multimedia services. For automatic piano transcription, the string inharmonicity coefficient (B) and fundamental frequency (f<SUB>0</SUB>) should be detected robustly and accurately. The proposed triplet-sequentially additive partial (SAP) algorithm improves the current B estimation algorithm in terms of both performance and speed with less prior knowledge. Additionally, this joint (B, f<SUB>0</SUB>) estimation algorithm is applied directly to the transcription of real piano recordings, and the 4.41% improvement of accuracy was achieved over another transcription system that had both similar processing steps and feature extraction method.</P>
Kim, Wonnam,Lee, Wonil,Choi, Jin Gyu,Ju, In Gyoung,Kim, Yun-Kyung,Lee, Tae Hee,Oh, Myung Sook Elsevier 2018 Journal of Ethnopharmacology Vol.215 No.-
<P><B>Abstract</B></P> <P><B>Ethnopharmacological relevance</B></P> <P>Aconiti Lateralis Radix Preparata (AR) is the most frequently used herb to generate heat and treat symptoms associated with coldness in Asia.</P> <P><B>Aims of the study</B></P> <P>The hypothalamus is one of the master regulators to maintain constant core body temperature. Chronic exposure to cold stress disturbs homeostatic regulation, gradually resulting in hypothalamic inflammation. This study investigate the effects of AR, on the chronic intermittent cold (CIC)-induced release of pro-inflammatory signaling molecules in the mouse hypothalamus.</P> <P><B>Materials and methods</B></P> <P>Aconiti Lateralis Radix Preparata extract (ARE) were solubilized in distilled water and diluted with saline before administration. Male ICR mice (7 weeks old, 30–32g) were divided randomly into 6 groups: (1) control, (2) cold stress, (3) ARE 30, (4) ARE 100, (5) ARE 300, and (6) ARE 1000mg/kg groups. Groups (2)-(6) were exposed to CIC stress once a day for 14 days. CIC stress was achieved by exposing the mice to 4°C and 60 ± 10% humidity for 120min once a day. Rectal temperature was measured after terminating cold stress. Cortisol levels were measured from serum. Hypothalamus tissue was used for western blot analysis, and IL-9, IL-13, PGE1, and PGE2 levels were assessed.</P> <P><B>Results</B></P> <P>ARE treatment prevented the CIC-induced decrease in rectal temperature and increase in serum cortisol level. ARE-treated CIC-exposed mice demonstrated decrease in nuclear c-Fos levels dose-dependently compared to CIC-exposed mice. Nuclear NF-kB expression showed significant increase in CIC-exposed mice. ARE treatment significantly blunted the increase in nuclear NF-kB expression. CIC-exposed mice had significantly increased levels of both IL-9 and IL-13. Treatment with ARE suppressed the elevated IL-9 and IL-13 levels. Between control and CIC-exposed mice PGE1 levels showed no difference. However ARE (1000mg/kg)-treated CIC-exposed mice had a significant increase in PGE1 level compared to CIC-exposed mice. PGE2 levels were significantly higher in CIC-exposed mice compared to control mice. ARE treatment significantly attenuated the increase in PGE2 levels.</P> <P><B>Conclusions</B></P> <P>Our findings suggest CIC stress disturbs the anti-inflammatory effect of cortisol and maintenance of the body temperature. Thus AR contributes to suppress the activated proinflammatory factors, IL-9, IL-13, and PGE-2, and to increase the heat production.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Kim, Seong Muk,Oh, Ji Hyeon,Park, Soon A,Ryu, Chung Heon,Lim, Jung Yeon,Kim, Dal-Soo,Chang, Jong Wook,Oh, Wonil,Jeun, Sin-Soo Wiley (John WileySons) 2010 Stem Cells Vol.28 No.12
<P>Irradiation is a standard therapy for gliomas and many other cancers. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is one of the most promising candidates for cancer gene therapy. Here, we show that tumor irradiation enhances the tumor tropism of human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) and the therapeutic effect of TRAIL delivered by UCB-MSCs. The sequential treatment with irradiation followed by TRAIL-secreting UCB-MSCs (MSC-TRAIL) synergistically enhanced apoptosis in either TRAIL-sensitive or TRAIL-resistant glioma cells by upregulating the death receptor 5 and by inducing caspase activation. Migration assays showed greater MSC migration toward irradiated glioma cells and the tumor site in glioma-bearing mice compared with unirradiated tumors. Irradiated glioma cells had increased expression of interleukin-8 (IL-8), which leads to the upregulation of the IL-8 receptor on MSCs. This upregulation, which is involved in the migratory capacity of UCB-MSCs, was confirmed by siRNA inhibition and an antibody-neutralizing assay. In vivo survival experiments in orthotopic xenografted mice showed that MSC-based TRAIL gene delivery to irradiated tumors had greater therapeutic efficacy than a single treatment. These results suggest that clinically relevant tumor irradiation increases the therapeutic efficacy of MSC-TRAIL by increasing tropism of MSCs and TRAIL-induced apoptosis, which may be a more useful strategy for cancer gene therapy.</P>
Processing Negative Polarity Items in Korean: Implications from an ERP Study
Myung-Kwan Park,Eunjeong Oh,Wonil Chung 서울대학교 언어교육원 2020 語學硏究 Vol.56 No.2
The present study used event-related potentials (ERPs) to examine the neural correlates supporting the processing of two negative polarity items (NPI) (nominal amwu+N+to “any+N+even” and adverbial te isang “any more/longer”) in Korean. Participants read sentences phrase by phrase while their brain activities during the processing of the NPIs and their licensors were being recorded. The results revealed that the NPI licensors in such contexts as positive or interrogative clauses not containing a proper licensor elicited a larger N400 component, possibly reflecting the cost of semantic or pragmatic integration. By contrast, sentences with the adverbial NPI te isang in the negative implicatureinducing “before” clause are not significantly different in ERP responses from those in the negative clause, thus evoking no ERP component. The present findings not only demonstrate semantic and pragmatic effects in neural signatures evoked by varied NPI-licensor relations, but also point to the multidimensionality of NPI processing/licensing recruiting semantic/pragmatic integration as well as syntactic dependency formation.