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Lung Cancer P-002 : MicroRNA-7-5p’s Role in Growth and Metabolism of Lung Cancer
( Sin Yung Woo ),( Daeun Kang ),( Su Yel Lee ),( Seong-lan Yu ),( Se Jin Park ),( Jin Suk Kim ),( Shin Yeop Lee ),( In Beom Jeong ),( Sun Jung Kwon ),( Wan Jin Hwang ),( Chang Ryul Park ),( Ji Woong S 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.-
OGlcNAc Transferase (OGT) is a complementary enzyme that regulates O-linked N-acetylglucosamine (O-GlcNAc) and plays a critical role in various cancer phenotypes including invasion, migration, and metabolic reprogramming. In this study, OGT is the predicted target of miR-7-5p. To identify miR-7-5p’s role in cell growth and metabolism, we treated various lung cancer cell lines with miR-7-5ps and performed qRT- PCR and western blot to demonstrate that OGT is the target of miR-7-5p. Furthermore, relative luciferase assay was done to show that miR-7-5p directly regulates OGT. To define the role of mir-7-5p in cancer cell metabolism, we performed LDH assay, glucose uptake, and mitochondrial ATP synthase inhibitor assay. Induced overexpression of miR-7-5p in lung cancer cells decreased aerobic glycolysis as is demonstrated by reduced LDH production, glucose uptake, and glycolysis. Increased O-GlcNAcylation contributed to the augmentation of lung cancer cell metabolism. In the present study, inhibition of OGT by miR7-5p decreased the growth and metabolism of lung cancer. These findings suggest that modulating OGT activity may present a potential therapeutic strategy for cancer.
Lee, Y.‐,S.,Choi, K.‐,M.,Choi, M.‐,H.,Ji, S.‐,Y.,Lee, S.,Sin, D.‐,M.,Oh, K.‐,W.,Lee, Y.‐,M.,Hong, J.‐,T.,Yun, Y.‐,P.,Yoo, H.‐,S. Blackwell Publishing Ltd 2011 Cell proliferation Vol.44 No.4
<P><B>Abstract</B></P><P><B>Objectives: </B> Melanoma is the most aggressive form of skin cancer, and it resists chemotherapy. Candidate drugs for effective anti‐cancer treatment have been sought from natural resources. Here, we have investigated anti‐proliferative activity of myriocin, serine palmitoyltransferase inhibitor, in the <I>de novo</I> sphingolipid pathway, and its mechanism in B16F10 melanoma cells.</P><P><B>Material and methods: </B> We assessed cell population growth by measuring cell numbers, DNA synthesis, cell cycle progression, and expression of cell cycle regulatory proteins. Ceramide, sphingomyelin, sphingosine and sphingosine‐1‐phosphate levels were analysed by HPLC.</P><P><B>Results: </B> Myriocin inhibited proliferation of melanoma cells and induced cell cycle arrest in the G<SUB>2</SUB>/M phase. Expressions of cdc25C, cyclin B1 and cdc2 were decreased in the cells after exposure to myriocin, while expression of p53 and p21<SUP>waf1/cip1</SUP> was increased. Levels of ceramide, sphingomyelin, sphingosine and sphingosine‐1‐phosphate in myriocin‐treated cells after 24 h were reduced by approximately 86%, 57%, 75% and 38%, respectively, compared to levels in control cells.</P><P><B>Conclusions: </B> Our results suggest that inhibition of sphingolipid synthesis by myriocin in melanoma cells may inhibit expression of cdc25C or activate expression of p53 and p21<SUP>waf1/cip1</SUP>, followed by inhibition of cyclin B1 and cdc2, resulting in G<SUB>2</SUB>/M arrest of the cell cycle and cell population growth inhibition. Thus, modulation of sphingolipid metabolism by myriocin may be a potential target of mechanism‐based therapy for this type of skin cancer.</P>
( Ji Young Jung ),( Myung Suk Choi ),( Ji Su Kim ),( Mi Jin Jeong ),( Young Wun Kim ),( Byeong Tae Woon ),( Jin Ki Yeo ),( Han Na Sin ),( Young Bon Goo ),( Keun Ok Ryu ),( Chandrakant S. Karigar ),( J 한국임학회 2010 한국산림과학회지 Vol.99 No.5
Enzymatic hydrolysate from non pre-treated biomass of yellow poplar (Liriodendron tulipifera) was prepared and used as resource for bioethanol production. Fresh branch (1 year old) of yellow poplar biomass was found to be a good resource for achieving high saccharification yields and bioethanol production. Chemical composition of yellow poplar varied significantly depending upon age of tree. Cellulose content in fresh branch and log (12 years old) of yellow poplar was 44.7 and 46.7% respectively. Enzymatic hydrolysis of raw biomass was carried out with commercial enzymes. Fresh branch of yellow poplar hydrolyzed more easily than log of yellow poplar tree. After 72 h of enzyme treatment the glucose concentration from Fresh branch of yellow poplar was 1.46 g/L and for the same treatment period log of yellow poplar produced 1.23 g/L of glucose. Saccharomyces cerevisiae KCTC 7296 fermented the enzyme hydrolysate to ethanol, however ethanol production was similar (~1.4 g/L) from both fresh branch and log yellow poplar hydrolysates after 96 h.
정상 혈청 총 IgE치를 보이는 알레르기 천식환자의 임상적 특성
최인선 ( In Seon S. Choi ),김옥기 ( Ok Ki Kim ),정성훈 ( Seong Hoon Jeong ),이우진 ( Won Jin Lee ),이성지 ( Seong Ji Lee ),신홍준 ( Hong Joon Sin ),한의령 ( Eui Ryoung Han ),정지민 ( Ji Min Jeong ) 대한천식알레르기학회 2009 천식 및 알레르기 Vol.29 No.2
Background: Although allergic diseases occur through IgE-mediated reaction, about 20% of allergic asthmatics have normal total serum IgE levels. Objective: To characterize the allergic asthmatics with normal total serum IgE levels. Method: Medical records of 430 consecutive allergic asthmatics, who received the tests for both total IgE and allergen-specific IgE in the serum or on the skin, were reviewed. According to the total and specific IgE levels, the subjects were classified to 4 groups (I, increased/strong; II, normal/strong; III, increased/weak; IV, normal/weak). Result: The Dermatophagoides farinae-specific IgE level in the serum was significantly related to the total serum IgE level (r=0.322, P<0.001). The D. farinae-specific IgE levels were significantly lower in the group II than in the group I (3.27±0.14 vs. 1.83±0.30, P<0.01) and the group IV than in the group III (0.79±0.05 vs. 0.44±0.13, P<0.01). The results for D. pteronyssinus and sum of checked allergens were similar. The proportion of the patients sensitized to single allergen to total subjects was 7.5%/13.5%/33.0%/40.0% for I/II/III/IV groups (χ2=44.5, P<0.001). Conclusion: The allergic asthmatics with normal total serum IgE levels had lower allergen-specific IgE levels and a higher proportion of the patients sensitized to single allergen. The levels of total serum IgE may be determined by the degree of sensitization in allergic asthma. (Korean J Asthma Allergy Clin Immunol 2009;29:96-104)
Predictive Mortality Index for Community-Dwelling Elderly Koreans
Kim, Nan H.,Cho, Hyun J.,Kim, Soriul,Seo, Ji H.,Lee, Hyun J.,Yu, Ji H.,Chung, Hye S.,Yoo, Hye J.,Seo, Ji A.,Kim, Sin Gon,Baik, Sei Hyun,Choi, Dong Seop,Shin, Chol,Choi, Kyung Mook Williams & Wilkins Co 2016 Medicine Vol.95 No.5
<▼1><P>Supplemental Digital Content is available in the text</P></▼1><▼2><P><B>Abstract</B></P><P>There are very few predictive indexes for long-term mortality among community-dwelling elderly Asian individuals, despite its importance, given the rapid and continuous increase in this population. We aimed to develop 10-year predictive mortality indexes for community-dwelling elderly Korean men and women based on routinely collected clinical data.</P><P>We used data from 2244 elderly individuals (older than 60 years of age) from the southwest Seoul Study, a prospective cohort study, for the development of a prognostic index. An independent longitudinal cohort of 679 elderly participants was selected from the Korean Genome Epidemiology Study in Ansan City for validation.</P><P>During a 10-year follow-up, 393 participants (17.5%) from the development cohort died. Nine risk factors were identified and weighed in the Cox proportional regression model to create a point scoring system: age, male sex, smoking, diabetes, systolic blood pressure, triglyceride, total cholesterol, white blood cell count, and hemoglobin. In the development cohort, the 10-year mortality risk was 6.6%, 14.8%, 18.2%, and 38.4% among subjects with 1 to 4, 5 to 7, 8 to 9, and ≥10 points, respectively. In the validation cohort, the 10-year mortality risk was 5.2%, 12.0%, 16.0%, and 16.0% according to these categories. The C-statistic for the point system was 0.73 and 0.67 in the development and validation cohorts, respectively.</P><P>The present study provides valuable information for prognosis among elderly Koreans and may guide individualized approaches for appropriate care in a rapidly aging society.</P></▼2>
( Kyoung-ho Song ),( Dong-min Kim ),( Hyunju Lee ),( Sin Young Ham ),( Sang-min Oh ),( Hyeonju Jeong ),( Jongtak Jung ),( Chang Kyung Kang ),( Ji Young Park ),( Yu Min Kang ),( Ji-yeon Kim ),( Jeong S 대한내과학회 2021 The Korean Journal of Internal Medicine Vol.36 No.1
Recently, the number of patients with coronavirus disease 2019 (COVID-19) who have tested positive for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), via the reverse transcription polymerase chain reaction (RT-PCR) test, after recovery has increased; this has caused a dilemma regarding the medical measures and policies. We evaluated the dynamics of viral load and anti-SARS-CoV-2 antibodies in four patients with positive RT-PCR results after recovery. In all patients, the highest levels of immunoglobulin G (IgG) and IgM antibodies were reached after about a month of the onset of the initial symptoms. Then, the IgG titers plateaued, and the IgM titers decreased, regardless of RT-PCR results. The IgG and IgM levels did not increase after the post-negative positive RT-PCR results in any of the patients. Our results reinforced that the post-negative positive RT-PCR results may be due to the detection of RNA particles rather than reinfection in individuals who have recovered from COVID-19.
Tai-Soon Yong,Eun-Hee Shin,Jong-Yil Chai,Woon-Mok Sohn,Keeseon S. Eom,Dong-Min Lee,Keunhee Park,Hoo-Gn Jeoung,Eui-Hyug Hoang,Yoon-Hee Lee,Hyun-Ju Woo,Ji-Hwa Lee,Sin-Il Kang,Jae-Ku Cha,Keon-Hoon Lee,Ch 대한기생충학열대의학회 2012 The Korean Journal of Parasitology Vol.50 No.2
Opisthorchis viverrini infection was found to be highly prevalent in 3 riverside villages (Ang Svay Chek A, B, and C) of the Prey Kabas District, Takeo Province. This area is located in the southern part of Cambodia, where the recovery of adult O. viverrini worms was recently reported. From May 2006 until May 2010, fecal examinations were performed on a total of 1,799 villagers using the Kato-Katz thick smear technique. In the 3 villages, the overall positive rate for helminth eggs ranged from 51.7 to 59.0% (av. 57.4%), and the percentage positive for O. viverrini was 46.4-50.6% (47.5%). Other helminths detected included hookworms (13.2%), echinostomes (2.9%), Trichuris trichiura (1.3%), Ascaris lumbricoides (0.6%), and Taenia spp. (0.06%). The prevalence of O. viverrini eggs appeared to reflect a lower infection in younger individuals (<20 years) than in the adult population (>20 years). Men (50.4%) revealed a significantly higher (P=0.02) prevalence than women (44.3%). The Ang Svay Chek villages of the Prey Kabas District, Takeo Province, Cambodia have been confirmed to be a highly endemic area for human O. viverrini infection.