Effect of interferon-γ on the fusion of mononuclear osteoclasts into bone-resorbing osteoclasts

( Jeung Woo Kim ),( Myeung Su Lee ),( Chang Hoon Lee ),( Ha Young Kim ),( Soo Uk Chae ),( Han Bok Kwak ),( Jae Min Oh ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.5

Osteoclasts are multinucleated cells that are formed by the fusion of pre-fusion osteoclasts (pOCs). The fusion of pOCs is known to be important for osteoclastic bone resorption. Here, we examined the effect of IFN-γ on the fusion of pOCs. IFN-γ greatly increased the fusion of pOCs in a dose-dependent manner. Furthermore, IFN-γ induced pOC fusion even in hydroxyapatite- coated plates used as a substitute for bone. The resorption area of pOCs stimulated with IFN-γ was significantly higher than that of the control cells. IFN-γ induced the expression of dendritic cell-specific transmembrane protein (DC-STAMP), which is responsible for the fusion of pOCs. IFN-γ enhanced DC-STAMP expression in a dose-dependent manner. The mRNA expression of c-Fos and nuclear factor of activated T cells (NFAT) c1 was enhanced in the pOCs treated with IFN-γ. Taken together, these results provide a new insight into the novel role of IFN-γ on the fusion of pOCs. [BMB reports 2012; 45(5): 281-286]

大豆갈색무늬병의 病進展과 品種間低抗性 檢定

JEUNG HAING OH 한국응용곤충학회 1985 한국응용곤충학회지 Vol.24 No.2

The present study was carried out to determine appropriate growth stage for evaluating resistance to septoria brown spot in field and to search resistance sources from soybean germ plasm. Disease severity expressed by log was different with soybean genotypes and vertical progress of the disease was related to the diseased leaf area. Correlation between diseased leaf area and the area under septoria brown spot disease progress curve (AUBC) was highest at full blooming stage, indicating a reasonable stage for measuring the disease severity to evaluate resistance in field. There was no lines highly resistant to the disease among 1,428 native soybean lines tested. 대두(大豆) 갈색무늬병에 대한 저항성(抵抗性)의 포장검정(圃場檢定)에 적합한 생육시기(生育時期)를 결정하고 수집재래종(蒐集在來種)으로부터 저항성인자원(抵抗性因子源)을 찾기 위 하여 본실험(本實驗)을 수행하였던 바, 1. 갈색무늬병에 대한 저항성(抵抗性) 품종간(品種間)에 차이(差異)가 현저하였으며 수직감염율(垂直感染率)과 병반면적율간(病斑面積率間)에는 상관(相關)이 있었다. 2. 병반면적율(病斑面積率)과 병진전곡선면적(病進展曲線面積)과의 상관도(相關度)는 대두(大豆) 생육기중(生育期中) 개화기(開花期)에서 가장 높아 포장에서의 저항성 검정시기는 개화기(開花期)가 적당한 것으로 보였다. 3. 수집재래종(蒐集在來種) 1,428계통의 저항성(抵抗性) 검정결과(檢定結果) 고도저항성(高度抵抗性) 계통(系統)은 발견할 수 없었고 중도저항성(中度抵抗性)인 4 계통(系統)을 선발할 수 있었다.

The Relation of Soybean Seedcoat Mottling with Podding Location and Seed Transmissibility in Soybean Mosaic Virus

Oh, Jeung-Haing The Korean Society of Crop Science 1984 Korean journal of crop science Vol.29 No.3

Association of SMV infection in soybean plants with seedcoat mottling and with seed transmission of SMV was examined. Seedcoat mottling was increased positively in proportion to the SMV severity in variety Clark. No correlation was found between the incidence of mottled seed and the seed's position on the plant. Susceptible variety with virus symptoms gave rise to mottled seeds but only a small percentage of these mottled seeds could transmit the virus. SMV could be transmitted even by nonmottled seeds harvested from SMV infected plants in similar rate with mottled seed. It seemed that the amount of mottled seed could be used as an indicator of the amounts of SMV infection in a seed production field. 콩모자이크 바이러스(SMV)에 의한 갈반립의 형성율과 SMV의 종자전염율을 조사하기 위하여 대두품종 Clark, Woodworth, 북해1호를 재료로 SMV 이병정도와 갈반립율 결협절위 및 갈반립형성등의 관계를 조사하고 그 갈반립을 통한 SMV의 종자전염율을 면역이중확산법과 유묘감염률로 조사하였다. 1. 갈반립의 형성율은 식물체의 이병정도와 정비례적인 상관(r=0.796)을 보였다. 2. 이병식물체의 갈반립형성은 결협절립 및 협중종실위치와 유의상관이 없었으며 (r=0.34), 동일절위 및 협내에서도 갈반립과 비갈반립이 함께 형성되었다. 3. 면역이중확산법으로 조사한 SMV의 종자전염율은 4.2 -33.3%로 비교적 낮았으며 품종에 따라 현저한 차이가 있었다. 4. 유묘에 나타난 SMV 증상으로 조사한 종자전염율은 면역이중확산법으로 검정한 종자감염율보다 약관 높게 나타났으며 이는 항혈청의 역가 또는 Virus의 체내증식과 관계가 있는 것으로 보였다. 5. 이병직물의 갈반립은 SMV 종자전염율과 일치하지는 않으나 SMV의 포장이병율 추정에 유용한 지표가 될 수 있으며 파종시 갈반립의 제거는 SMV 전염원의 감소효과를 가져올 수 있을 것으로 보였다.

Efficacy of Fluazinam and Iprodione+Propineb in the Suppression of Diaporthe phaseolorum, Colletotrichum truncatum and Cercospora kikuchii, the Causal Agents of Seed Decay in Soybean

Oh, Jeung-Haing,Kang, Nag-Won The Korean Society of Plant Pathology 2002 Plant Pathology Journal Vol.18 No.4

Seed decay of soybean caused by Diaporthe phaseolorum, Colletotrichum truncatum and Cercospora kikuchii is a serious disease when soybean is harvested under warm and wet weather conditions. Benomyl has been used for controlling the disease, however, benomyl application may be limited due to common occurrence of resistance. The efficacy of 21 fungicides against the pathogens was evaluated in vitro. Among the fungicides tested, benomyl, carbendazim, fluazinam, iprodione+propineb, thiophanate-methyl, and triflumizole were found effective and were evaluated for their ability to control the seed pathogens. Fluazinam completely inhibited mycelial growth at a concentration of 100 $\mu\textrm{g}$/$\textrm{m}{\ell}$ for D. phaseolorum; and at a concentration of 500 $\mu\textrm{g}$/$\textrm{m}{\ell}$ for C. truncatum and C. kikuchii. $EC_90$ values of fluazinam were similar to that of benomyl. Because fluazinam, iprodione+propineb, and triflumizole were found effective against the seed pathogens, these were subjected for field-testing. Suppression of pod and seed infection by fluazinam and iprodione+propineb was as high as that of benomyl without any reduction in agronomic characters of soybean. This study shows that fluazinam and iprodione+propineb may be used in combination with benomyl to control seed pathogens, manage resistance, and ensure production of high quality soybean seeds.

分生胞子數 및 營養狀態가 대두갈색무늬병균의 分生胞子 發芽에 미치는 影響

Jeung Haing Oh,Hoo Sup Chung 한국응용곤충학회 1984 한국응용곤충학회지 Vol.23 No.1

대두갈색무늬병균의 포자발아(分生胞子發芽) 미치는 외부영양공급(外部營養供給) 및 self-inhibitor의 영향(影響)을 조사하여 다음과 같은 결과(結果)를 얻었다. 1. 갈색무늬병균의 포자발아율은 감자한천배지 및 대두잎조각을 첨가한 증류수에서는 양호하였으나 살균된 증류수에서는 극히 불량하였다. 2. 갈색무늬병균의 포자발아에는 탄소원의 외부공급이 절대 필요한 것으로 보였으며 인산, 가리 등은 큰 영향이 없는 것으로 보였다. 3. 탄소원으로서는 가용성 전분이 가장 효과적이었고 다음이 포도당, 유당 등이었으며 포도당의 경우 농도에서 가장 높은 발아율을 보였다. 4. 포자발아율은 포자밀도가 높을수록 현저히 감소하여 포자농도 이상에서는 거의 발아하지 않는 것으로 보아 self-inhibitor가 존재하는 것으로 보였다. 5. 기질(基質)을 세척한 포자의 발아율은 세척하지 않은 포자의 발아율에 비해 낮았으며 이러한 현상은 포자농도가 이상으로 증가할 때 더욱 현저하였고 건조된 한천배양기위에는 발아율 감소가 현저하였다. Conidial germination of Septoria glycines Hemmi, brown spot fungus of soybean, was studied by slide germination test. Poor conidial germination of S. glycines was observed on sterile distilled water, but potato dextrose agar(PDA) and distilled water floated with soybean leaf disc furnished a satisfactory medium for conidial germination. Exogenous supply of carbon source was essential for conidial germination, while phosphorous and potassium were not evident as that for carbon. Soluble starch was the most suitable as a carbon source for conidial germination and followed by D-glucose, D-galactose and lactose in that order. Maximum germination was attained in the . concentration of glucose. Germination was decreased with increment of conidial concentration and was almost completely suppressed in the density of 10,000 conidia per . It suggested existing a self-inhibitor(s). Non-washed conidia germinated more than washed conidia and this was obvious when the conidia density was over conidia per on the dry agar block.

여름콩의 開花 · 成熟 및 形態的 特性變異

Yong Ha Chu(朱勇河),Jeung Haing Oh(吳正行),Je Cheon Chae(蔡濟天),Yeaul Kyu Seung(成烈圭),Beom Young Son(孫範永),Kil Woong Chung(鄭吉雄) 한국육종학회 1996 한국육종학회지 Vol.28 No.3

This experiment was conducted to select useful breeding materials among eighty lines or varieties of summer type soybeans tested in the experiment field, DanKook University, Cheonan in 1993. Fourty-four to sixty-two days were observed as the range of days from planting to flowering and the average was 53 days. The range of days from planting to maturity was 112 to 127 days and the average was 117 days. Eighty-six percentage of tested soybean materials was white color of flower and green color of hypocotyl, while the remains were purple in both. Soybean entries with ovate, long circular and circular type of leaf were 6%, 73% and 21%, respectively. Large, medium and small leaf in the leaf area were 34%, 32% and 34%, respectively. Light green leaf color was observed as 15%, and green and dark green color were 70% and 15% among tested soybeans, respectively. Soybeans with tan pods were observed as 49%, brown and dark brown color were 14% and 37%. Erect type of pubescence was 36%, whereas curly and appressed type were 54% and 10%, respectively. Sparse, normal and dense pubescence density were 16%, 41% and 43%, respectively. Most soybean entries(63%) showed gray pubescence and the remains were brown color. Days from planting to flowering(DPF) showed highly negative correlation with days from flowering to maturity(DFM) and days from planting to maturity (DPM), whereas it was highly positively correlated with leaf area. Days from flowering to maturity was highly positively associated with days from planting to maturity, while it showed highly negatively correlated with leaf area. Days from planting to maturity was highly negative correlation with leaf area.

EFFECTS OF LEAF MATURITY ON THE DISEASE PROGRESS OF SEPTORIA BROWN SPOT IN SOYBEAN

오정행,Oh Jeung Haing The Korean Society of Plant Pathology 1987 Plant Pathology Journal Vol.3 No.4

대두 갈색무늬병의 진전은 한 식물체에서 상위엽으로의 진전속도(수직감염율 : vertical progress)과 밀접한 관계가 있다. 실제로 포장에서의 갈색무늬병 진전은 주로 하위엽에서 발병하기 시작하여 상위엽에 비하여 이병정도가 높은 것이 보통이다. 이러한 현상의 원인을 구명하기 위하여 수행한 본 실험에서 갈색무늬병의 감염율은 잎의 성숙도와 정의 상관을 보였으며 이러한 유엽저항성은 접종원의 분포와는 무관한 것으로 보였다. 또 상위엽의 엽표면일출액 (leaf diffusate)은 병원균 포자발아 및 관계신장의 억제 정도가 하위엽의 일출액에 비하여 높았으며 이러한 경향은 저항성품종에서 더욱 현저하여 엽일출액의 어떤 발아억제물질이 갈색무늬병의 유엽저항성에 관여하는 것으로 추정되었다. Septoria brown spot caused by Septoria glycines Hemmi is one of the serious fungal diseases in soybean. Since little has been known about the disease progress in the field, the present study was conducted to determine the factors affecting the disease progress in the soybean plant. Disease severity and pattern of the progress of the Septoria brown spot were different with varieties. Susceptibility of soybean plants increased with increase of plant age and leaf maturity in order from the primary leaf to the newly expanded leaf. It seemed to be related with conidial germination on the leaves. Germination and germtube elongation were more inhibited by the diffusates obtained from upper leaves than those from lower leaves and they were higher in a susceptible variety than in a moderately resistant one.

부동산 중개업자의 직업윤리와 일반적 특성에 관한 연구

오인종(Oh, In Jong),하정순(Ha, Jeung Soon),이창석(Lee Chang Suck) 한국부동산학회 2011 不動産學報 Vol.45 No.-

1. CONTENTS (1) RESEARCH OBJECTIVES This study aims to find influential variables in terms of correlation of work ethic and general features with necessity of work ethic. (2) RESEARCH METHOD The analytical methods used in this study were frequency, mean, Factor Analysis, t-test, ANOVA, post-hoc estimation(Duncan test), multiple regression. To verify the reliability of each measure, Cronbach’s alpha coefficient was used. (3) RESEARCH FINDINGS Upon the result of the study, work ethic elements were found as service, organization, employment and public ethics. Among those, the most enthusiastically pursed one by agents was service ethic while the least one was public ethic. 2. RESULTS The more they pursue for public ethic, the more strongly they feel a need of work ethic. Those who possess real estate license feel the necessity of work ethic most strongly, followed by representative and employees who both have certificates and those with long career.

포도 흰가루병균(Uncinula necator)의 분생포자 형성과 발아에 미치는 온도, 습도, pH 및 Trizole 살균제의 영향

오정행,Oh, Jeung-Haing 한국식물병리학회 1997 Plant Pathology Journal Vol.13 No.4

포도 흰가루병균(Uncinula necutor)의 분생포자 형성, 발아 및 균사 초기생장에 미치는 중요 환경오인과 살균제의 영향을 구명하기 위하여 수행한 연구 결과를 요약하면 다음과같다. 분생포자 형성은 상대습도 76~96%에서 가장 좋았다. 포자 발아 및 균사 초기생장은 증류수에 비해 5% 한천배지에서 좋았으며, 온도 $26^{\circ}C$, pH 5.0일 때 가장 좋았다. 광 100 lux는 암상태와 발아율에 차이가 없었으며 한천배지에 포도 엽즙액 30%를 첨가한 배지에서는 순수 한천배지 보다 좋았다. 또 triazole계 살균제 첨가 배지에서의 포자발아 및 균사생장은 살균제 농도증가에 비례하여 급격히 감소하였으나 그 정도는 살균제의 종류에 따라 차이가 있었다. 특히, myclobutanil은 농도증가에 따른 발아율의 감소정도가 매우 낮았다. The experiment was conducted to obtain basic informations on the effects of key environments and fungicides on the sporulation, conidial germination and hitial growth of the hyphae of grape powdery mildew fungus Uncinula necator. Maximum sporulation occurred at RH 75~96% in vitro adiusted with sulfuric acid solution. Conidial germination and initial growth of the hyphae were better at 5% water agar than distilled water, and best at $26^{\circ}C$ and pH 5.0 of the substrates. Germination occurred equally well at light intensity of below 100 lux or dark condition, which was better at water agar supplemented with leaf extract 30% than pure water agar. The water agar supplemented with triazole fungicides reduced conidial germinations and initial growth of the hyphae signifcantly, in proportion to the increase in concentration of the fungicides, but the maguitude of reduction depended on the fungicides. Particularly in myclobutanil, reduction rate was very low as increased in concentration.

GSM 휴대폰 TDMA 잡음 전달 특성 분석을 통한 통화 품질 개선에 관한 연구

하정욱(Jeung-Uk Ha),오태훈(Tae-Hoon Oh),강진석(Jin-Seok Kang),윤영중(Young-Joong Yoon) 한국전자파학회 2008 한국전자파학회논문지 Vol.19 No.4

본 논문에서는 GSM(Global System for Mobile communication) 휴대폰에서 TDMA(Time Division Multiple Access)잡음의 원인 및 TDMA 잡음원의 판별 방법에 대해서 기술한다. TDMA 잡음의 원인은 RF(Radio Frequency)에너지 결합과 버스트 리플(Burst ripple)에 의한 저주파 에너지 결합으로 구성된다. TDMA 잡음원의 판별 방법을 출력(TDMA 잡음 측정)과 시스템(오디오 경로)의 주파수 응답을 통해 제안한다. 특히 RF 에너지 결합에 대해 삽입 손실(S21) 분석 방법과 개선 방법을 제안한다. RF 에너지 결합을 줄이기 위해 커패시터(40 pF)가 해결법이고, 결과적으로 TDMA 잡음이 10 ㏈가 줄어들었다. In this paper, we describe the cause of TDMA noise and distinction method of TDMA noise source in a GSM mobile phone. The causes of TDMA noise are composed of RF(Radio Frequency) energy coupling and low frequency energy coupling by burst ripple. We propose the distinction method of TDMA noise source from output(TDMA noise measurement) and frequency response of a system(audio path). Especially we propose a method of insertion loss(S21) analysis and the improvement method for RF energy coupling. Capacitor(40 pF) is a solution to reduce RF energy coupling and therefore TDMA noise was reduced by 10 ㏈.