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( Won Chull Bak ),( Ji Heon Park ),( Young Ae Park ),( Kang Hyeon Ka ) 한국균학회 2014 Mycobiology Vol.42 No.3
Shiitake mushrooms (Lentinula edodes) containing β-glucans may be beneficial for human health; they have been used in the treatment of cancer, hypertension, and high cholesterol levels. The objective of this study was to determine the β-glucan content in different sections of the fruiting bodies and mycelia of ten shiitake mushroom cultivars. The measured β-glucan content ranged from 20.06 ± 1.76% to 44.21 ± 0.13% in the pileus sections, and from 29.74 ± 1.40% to 56.47 ± 4.72% in the stipe sections. The results of this study indicate that the variance in β-glucan content dependent on the shiitake cultivar, and that the β-glucan content is higher in the stipe than in the pileus.
Won-Chull Bak,Bong-Hun Lee,Young-Ae Park,Hyun-Seok Kim 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4
A harmful fungus occurred seriously in bed-log of shiitake(Lentinula edodes) in Jangheung-Gun, Korea. The fungus was identified as Bjerkandera adusta by its morphology and ITS(Internal Transcribed Spacer) analysis. The fungus was reported as causal agent of stem-rot of Populus euramericana in Korea, but not reported in bed-log of shiitake until this notification. Thus, studies were made to investigate inside condition of bed-log of shiitake damaged by B. adusta, physiological characteristics of B. adusta and antagonism between these two fungi. First of all, B. adusta is white-rotting fungus like shiitake and wood-rotting condition is similar to that of shiitake. But, there are a lot of small spots in damaged wood tissue under bark which are not seen in case of shiitake. Optimal temperature for mycelial growth of B. adusta is ca. 30℃ while that of shiitake is ca. 25 ℃. When confrontation cultures were made between these two fungi under 15℃, 20℃, 25℃ and 30℃, B. adusta has antagonistic ability against shiitake in all the temperatures. From the results of experiments, if the bed-logs of shiitake are exposed to high temperature, there should be mass propagation of B. adusta, and shiitake mycelia will be seriously injured by the fungus. Therefore, to prevent the damage by B. adusta, it is needed to grow the mycelia of shiitake fast in the bed-log, and to avoid exposure of the bed-log to high temperature in summer.
박원철 ( Won Chull Bak ),박영애 ( Young Ae Park ),이봉훈 ( Bong Hun Lee ),가강현 ( Kang Hyeon Ka ),박지헌 ( Ji Heon Park ) 한국균학회 2013 韓國菌學會誌 Vol.41 No.1
New Shiitake (Lentinula edodes) strain “Chunbaegko” was bred by Di-mon method. Bed-log cultivation of “Chunbaegko” was performed. Fruit-body production of “Chunbaegko” was most at spring and autumn. Optimal temperature of fruit-body formation was 14~22oC Baegwhako, the best quality fruit-body, is produced during spring. The diameter of pileus is ca. 52 mm. The total amount of fruit-body production during 4 years(one generation) was 140 kg/m3 log.
Berberine Sulphate 를 이용한 형광현미경기법에 의한 수목마이코플라스마검정
박원철(Won Chull Bak),나용준(Yong Joon La) 한국산림과학회 1991 한국산림과학회지 Vol.80 No.2
The efficacy of berberine sulphate, a fluorochrome having binding properties with both DNA and RICA, was investigated for the detection of mycoplasma-like organisms(MLOs) in jujube(Zizyplzus jujuba), paulownia (Paulounia tomentosa), mulberry(Morus alba) trees and periwinkle (Catharantlaus roseus) plant. When examined under fluorescence microscope, berberine sulphate-stained sections of diseased samples showed distinct MLO-specific fluorescent particles in the phloem area, while such fluorescence was absent in the healthy ones. This staining technique was proved to be a very accurate method for the diagnosis of MLO infections in woody and herbaceous plants. Furthermore, the cheap and easy procedure could be used to test a, great number of samples on MLO infections with reliability and rapidity.
옥시테트라싸이클린의 수확후수간주입에 의한 대추나무 빗자루병 방제 (防除)
박원철(Won Chull Bak),나용준(Yong Joon La) 한국산림과학회 1993 한국산림과학회지 Vol.82 No.1
Post-harvest trunk injection of oxytetracycline-HCl (OTC) was attempted for the control of jujube witches` -broom. Diseased jujube(Zizyphus jujzuba) trees with 9 to 16㎝ trunk diameters were treated with OTC ranged from 2g to 6g according to the size of trunk diameter. OTC dissolved in 0.51 or 1.01 of water was transfused into diseased trees by gravity flow injection during September and October. All these OTC concentrations prevented symptom development for two growing seasons and restored previously severely diseased trees to normal conditions. There was no differences in control effect between 0.51 and 1.01 OTC solutions. With these high OTC concentrations, no phytotoxicity was observed in the new leaves of the following year. Mycoplasma -like organism (MLO)-specific fluorescence was absent in the phloem of recovered tissue when examined by fluorescence microscopy using DAPI (4`-6-diamidino-2-phenylindole.2HCl) staining, indicating the disappearance of MLO by the action of OTC.