유한요소법을 이용한 Wire Rope Type Wheel Carrier 부품의 구조해석

염상훈(Sanghoon Yeom),이성범(Seong Beom Lee),한정훈(Jeonghun Han) 대한기계학회 2012 대한기계학회 춘추학술대회 Vol.2012 No.5-2

유한요소법을 이용한 스패어 휠 캐리어용 토크리미터의 해석

염상훈(Sanghoon Yeom),이성범(Seong Beom Lee),한정훈(Jeonghun Han) 한국자동차공학회 2012 한국자동차공학회 부문종합 학술대회 Vol.2012 No.5

Existing spare wheel carrier in way of Link chain cause accidents like fall while driving because Link chain has several problems such as tangle, damage. To solve this problem, we hope to develop spare wheel carrier in the way of Wire rope. In significant part which has complex mechanism like Torque limiter, Anti turn around device, Anti loosen device in Wire rope type wheel carrier, analysis using FEM(Finite Element Method) is required because simple theoretical calculation has high risk in trial and error. Torque limiter is one of important part to prevent Spare wheel"s falling when cam shaft has too much Torque. In this study, we want to verify pilot design through analysis of Torque limiter using FEM. For this, we will compare and analyze the torque in Torque limiter by using ANSYS which is FEA(Finite Element Analysis) software.

Comparative Analysis of Proteomes and Phosphoproteomes in Patients with Prostate Cancer Using Different Surgical Conditions

Ahn Hee-Sung,Yeom Jeonghun,Jeong Hwangkyo,Park Won Young,Ku Ja Yoon,Kang Byeong Jin,Kim Kyung Hwan,Lee Chan Ho,Song Sangheon,Bae Sun Sik,Kim Kyunggon,Ha Hong Koo 대한남성과학회 2022 The World Journal of Men's Health Vol.40 No.4

Purpose: To establish the standard of procedure in preparing benign and cancerous prostate tissues and evaluate the quality of proteomics and phosphoproteomics during transurethral resection of the prostate (TUR-P) with different surgical conditions. Materials and Methods: TUR-P tissue samples from three patients, two diagnosed with prostate cancer and one with benign prostatic hyperplasia, were each analyzed under three different conditions, based on differences in energy values, tissue locations, and surgical techniques. Global- and phosphorylated proteomic profiles of prostate tissues were analyzed by liquid chromatography-tandem mass spectrometry. Results: A total of 6,019 global proteins and 4,280 phosphorylated peptides were identified in the nine tissues. The quantitative distributions of proteins and phosphorylation in tissues from the same patient were not affected by changes in the surgical conditions, but indirect relative comparisons differed among patients. Phosphorylation levels, especially of proteins involved in the androgen receptor pathway, important in prostate cancer, were preserved in each patient. Conclusions: Proteomic profiles of prostate tissue collected by TUR-P were not significantly affected by energy levels, tissue location, or surgical technique. In addition, since protein denaturation of samples through TUR-P is rarely confirmed in this study, we think that it will be an important guide for tissue samples in castration resistant prostate cancer patients, where it is difficult to obtain tissue. This result is the first report about proteomic and phosphoproteomic results with TUR-P samples in prostate cancer and will be theoretical basis in protein analysis research with prostate cancer tissues.

High-throughput peptide quantification using mTRAQ reagent triplex

Yoon, Joo Young,Yeom, Jeonghun,Lee, Heebum,Kim, Kyutae,Na, Seungjin,Park, Kunsoo,Paek, Eunok,Lee, Cheolju BioMed Central 2011 BMC bioinformatics Vol.12 No.suppl1

<P><B>Background</B></P><P>Protein quantification is an essential step in many proteomics experiments. A number of labeling approaches have been proposed and adopted in mass spectrometry (MS) based relative quantification. The mTRAQ, one of the stable isotope labeling methods, is amine-specific and available in triplex format, so that the sample throughput could be doubled when compared with duplex reagents.</P><P><B>Methods and results</B></P><P>Here we propose a novel data analysis algorithm for peptide quantification in triplex mTRAQ experiments. It improved the accuracy of quantification in two features. First, it identified and separated triplex isotopic clusters of a peptide in each full MS scan. We designed a schematic model of triplex overlapping isotopic clusters, and separated triplex isotopic clusters by solving cubic equations, which are deduced from the schematic model. Second, it automatically determined the elution areas of peptides. Some peptides have similar atomic masses and elution times, so their elution areas can have overlaps. Our algorithm successfully identified the overlaps and found accurate elution areas. We validated our algorithm using standard protein mixture experiments.</P><P><B>Conclusions</B></P><P>We showed that our algorithm was able to accurately quantify peptides in triplex mTRAQ experiments. Its software implementation is compatible with Trans-Proteomic Pipeline (TPP), and thus enables high-throughput analysis of proteomics data.</P>

Euchromatin histone methyltransferase II (EHMT2) regulates the expression of ras-related GTP binding C (RRAGC) protein

Supyong Hwang,김소영,Kyungkon Kim,Jeonghun Yeom,Sojung Park,Inki Kim 생화학분자생물학회 2020 BMB Reports Vol.53 No.11

Dimethylation of the histone H3 protein at lysine residue 9 (H3K9) is mediated by euchromatin histone methyltransferase II (EHMT2) and results in transcriptional repression of target genes. Recently, chemical inhibition of EHMT2 was shown to induce various physiological outcomes, including endoplasmic reticulum stress-associated genes transcription in cancer cells. To identify genes that are transcriptionally repressed by EHMT2 during apoptosis, and cell stress responses, we screened genes that are upregulated by BIX-01294, a chemical inhibitor of EHMT2. RNA sequencing analyses revealed 77 genes that were upregulated by BIX-01294 in all four hepatic cell carcinoma (HCC) cell lines. These included genes that have been implicated in apoptosis, the unfolded protein response (UPR), and others. Among these genes, the one encoding the stress-response protein Ras-related GTPase C (RRAGC) was upregulated in all BIX-01294-treated HCC cell lines. We confirmed the regulatory roles of EHMT2 in RRAGC expression in HCC cell lines using proteomic analyses, chromatin immune precipitation (ChIP) assay, and small guide RNA-mediated loss-of-function experiments. Upregulation of RRAGC was limited by the reactive oxygen species (ROS) scavenger N-acetyl cysteine (NAC), suggesting that ROS are involved in EHMT2-mediated transcriptional regulation of stress-response genes in HCC cells. Finally, combined treatment of cells with BIX-01294 and 5- Aza-cytidine induced greater upregulation of RRAGC protein expression. These findings suggest that EHMT2 suppresses expression of the RRAGC gene in a ROS-dependent manner and imply that EHMT2 is a key regulator of stress-responsive gene expression in liver cancer cells.

Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer.

Jung, Ji-Han,Kim, Hye-Jung,Yeom, Jeonghun,Yoo, Changyoung,Shin, Jihye,Yoo, Jinyoung,Kang, Chang Suk,Lee, Cheolju Springer International 2012 Journal of gastroenterology Vol.47 No.1

<P>Lymph node metastasis (LNM) is recognized as an important factor in the progression of tumor malignancy. It is required to discover molecular markers for the prediction of LNM in gastric cancers (GCs).</P>

Synergistic effect of ulipristal acetate and vitamin D with HIFU on uterine leiomyoma

( Somi Oh ),( Ku Heon Kwon ),( Jeonghun Yeom ),( Kyunggon Kim ),( Yong-il Kwon ) 대한산부인과학회 2020 대한산부인과학회 학술대회 Vol.106 No.-

Objective: Uterine leiomyoma (UL), are noncancerous growths of the uterus that often appear during childbearing years and also called uterine fibroids. Recently, combination of ulipristal acetate (UPA) and high intensity focused ultrasound (HIFU) have been applied on UL treatment and cholecalciferol have been also suggested as a synergistic component but no pathway of efficacy was investigated. Herein, comparative proteome approach on leiomyoma cell line model was applied to investigate related pathways for UA treatment based on HIFU. Methods: Uterine leiomyoma cell line (GM10964) was treated with 10uM of ulipristal acetate (UPA) or with 10uM of UPA and 1uM of cholecalciferol(VitD3), respectively. After 24-hour incubation, cell was treated with 200W of HIFU using JC200D with home-made mount on transducer. And quantitative proteome analysis for each cell was performed using high resolution LC-MS platform. Results: Totally 5,286 proteins are identified among groups and gene ontology analysis showed that proteins which increased in HIFU combined with UPA and VitD3 were involved in mitochondrial metabolism, inferring apoptosis for cell death. Conclusion: Comparative proteome analysis revealed that combination of UPA and VitD3 on HIFU treatment for uterine leiomyoma showed the synergistic effect in term of apoptosis caused by mitochondrial metabolism.

Comparative profiling of breast cancer tissue membrane proteome by use of SDS-PAGE based cICAT method

Seong-Jun Park,Joohee Mun,Won Suk Yang,Mohammad Humayun Kabir,Jeonghun Yeom,Jihye Shin,Hee-Sung Ahn,Shinyeong Joo,Yu Mi Kwon,Mijeong Kim,Dong Huey Cheon,Su-Min Lee,Yae Eun Park,Kyeong Yeon Kim,Ji Eun 한국구조생물학회 2015 Biodesign Vol.3 No.1

Breast cancer is the most common cancer among women worldwide. There is an emerging interest in protein expression profiling with the aim of identifying novel diagnostic markers and therapeutic targets in breast cancer. We analyzed breast cancer tissues by using the cICAT (cleavable isotope-coded affinity tag) labeling approach and tandem mass spectrometry. Breast cancer and matched normal tissues were fractionated by ultracentrifugation to enrich membrane proteins, and cICAT labeled proteins were separated by SDS-PAGE. A total of 364 proteins were identified and quantified. Among the proteins that showed >1.5 fold difference between normal and cancer (36.6%), five (RPN1, ERp29, Trop2, PrP and PIGR) were selected for confirmation. Western blot on 10 pairs of normal and cancer tissues revealed that the expression of RPN1 and ERp29 were increased in breast cancer tissue, while Trop2, PrP and PIGR were decreased. Those Western blot results were highly consistent with cICAT results. Higher level of RPN1 and ERp29 and lower level of Trop2 in breast cancer cell lines compared to normal breast cell lines were observed. The result supports reliability of our SDS-PAGE based ICAT method in quantifying cancer tissue proteome.

Formyl-methionine as an N-degron of a eukaryotic N-end rule pathway

Kim, Jeong-Mok,Seok, Ok-Hee,Ju, Shinyeong,Heo, Ji-Eun,Yeom, Jeonghun,Kim, Da-Som,Yoo, Joo-Yeon,Varshavsky, Alexander,Lee, Cheolju,Hwang, Cheol-Sang American Association for the Advancement of Scienc 2018 Science Vol.362 No.6418

<P><B>Another N-end rule to add</B></P><P>Proteins that emerge from a ribosome bear the N-terminal methionine (Met) residue. In bacteria, Met is formylated before translation starts, whereas in eukaryotes, most nascent proteins seemed to start with unmodified Met. Working in yeast, Kim <I>et al.</I> found that the N-terminal formylation of eukaryotic proteins is detectable even under normal conditions and is greatly increased upon specific stresses, which cause some Fmt1 formyltransferase to be retained in the cytoplasm. The retention of this normally mitochondrial protein was found to require the Gcn2 kinase. In addition, the Psh1 ubiquitin ligase was shown to target N-terminally formylated eukaryotic proteins for proteasome-dependent degradation by the so-called fMet/N-end rule pathway.</P><P><I>Science</I>, this issue p. eaat0174</P><P>In bacteria, nascent proteins bear the pretranslationally generated N-terminal (Nt) formyl-methionine (fMet) residue. Nt-fMet of bacterial proteins is a degradation signal, termed fMet/N-degron. By contrast, proteins synthesized by cytosolic ribosomes of eukaryotes were presumed to bear unformylated Nt-Met. Here we found that the yeast formyltransferase Fmt1, although imported into mitochondria, could also produce Nt-formylated proteins in the cytosol. Nt-formylated proteins were strongly up-regulated in stationary phase or upon starvation for specific amino acids. This up-regulation strictly required the Gcn2 kinase, which phosphorylates Fmt1 and mediates its retention in the cytosol. We also found that the Nt-fMet residues of Nt-formylated proteins act as fMet/N-degrons and identified the Psh1 ubiquitin ligase as the recognition component of the eukaryotic fMet/N-end rule pathway, which destroys Nt-formylated proteins.</P>

Regulation of IκB Kinase by GβL through Recruitment of the Protein Phosphatases

Dong-Joo You,You Lim Kim,Cho Rong Park,Dong-Kyu Kim,Jeonghun Yeom,이철주,Curie Ahn,Jae Young Seong,Jong-Ik Hwang 한국분자세포생물학회 2010 Molecules and cells Vol.30 No.6

protein β-like (GβL) is a member of WD repeat-containing family which are involved in various intracellular signaling events. In our previous report, we demonstrated that GβL regulates TNFα-stimulated NF-κB signaling by interacting with and inhibiting phosphorylation of IκB kinase. However, GβL itself does not seem to regulate IKK directly, because it contains no functional domains except WD domains. Here, using immunoprecipitation and proteomic analyses, we identified protein phosphatase 4 as a new binding partner of GβL. We also found that GβL interacts with PP2A and PP6, other members of the same phosphatase family. By interacting with protein phosphatases,which do not directly bind to IKKβ, GβL mediates the association of phosphatases with IKKβ. Overexpression of protein phosphatases inhibited TNFκ-induced activation of NF-κB signaling, which is an effect similar to that of GβL overexpression. Down-regulation of GβL by small interfering RNA diminished the inhibitory effect of phosphatases, resulting in restoration of NF-κB signaling. Thus, we propose that GβL functions as a negative regulator of NF-κB signaling by recruiting protein phosphatases to the IKK complex.