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Response of Calcineurin B-Like Protein Kinase Gene in Tomato to Various Abiotic Stresses
( Mohammad Humayun Kabir ),( Myeong Hyeon Wang ) 한국응용생명화학회 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.1
This study describes the expression patterns of the CBL interacting protein kinase (SlCIPK) gene in Solanum lycopersicum after treatment with various abiotic stresses, such as salt, dehydration and mechanical wounding. The cDNA of the SlCIPK gene from the tomato leaf encodes 446 amino acids and shows significant homology with other plant CIPK proteins. SlCIPK is a single copy gene in the tomato genome. The organ-specific expression pattern of the SlCIPK gene revealed that the transcript of this gene was present in all tissues examined (roots, stems, leaves and flowers), with strong expression being observed in the roots. Additionally, the SlCIPK gene was upregulated in response to salt, dehydration and wounding. Moreover, SlCIPK was regulated by abscisic acid (ABA) and calcium. Taken together, these results indicate that SlCIPK gene may play a role in the response to abiotic stresses via ABA-mediated calcium signaling.
Response of Calcineurin B-like Protein Kinase Gene in Tomato to Various Abiotic Stresses
Kabir, Mohammad Humayun,Wang, Myeong-Hyeon The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.1
This study describes the expression patterns of the CBL interacting protein kinase (SlCIPK) gene in Solanum lycopersicum after treatment with various abiotic stresses, such as salt, dehydration and mechanical wounding. The cDNA of the SlCIPK gene from the tomato leaf encodes 446 amino acids and shows significant homology with other plant CIPK proteins. SlCIPK is a single copy gene in the tomato genome. The organ-specific expression pattern of the SlCIPK gene revealed that the transcript of this gene was present in all tissues examined (roots, stems, leaves and flowers), with strong expression being observed in the roots. Additionally, the SlCIPK gene was upregulated in response to salt, dehydration and wounding. Moreover, SlCIPK was regulated by abscisic acid (ABA) and calcium. Taken together, these results indicate that SlCIPK gene may play a role in the response to abiotic stresses via ABA-mediated calcium signaling.
Kabir, Mohammad Humayun,Wang, Myeong-Hyeon The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.6
Dehydroascorbate reductase (DHAR) plays an important role in the ascorbate-glutathione cycle in plants by controlling the homeostasis of reactive oxygen species. Expression profiles of two DHAR genes (SlDHAR1 and SlDHAR2) in tomato leaves were analyzed in response to abiotic and oxidative stresses as well as their expression in various organs were investigated under normal growth conditions. Multiple copies of SlDHAR homologues exist in the tomato genome. SlDHAR1 expression was dominant in stems and flowers but moderate in leaves; however, SlDHAR2 induction was strong in stems, leaves, and flowers, and young leaves showed the highest expression of SlDHAR2. Both genes were transcribed after treatment with salt, dehydration, and methyl viologen. Prolonged stress reduced SlDHAR genes expression. The level of hydrogen peroxide increased, and DHAR activity was altered by stress treatment. Results indicate that SlDHAR genes could play a role in multiple stress responses.
Mohammad Humayun Kabir,한웅,왕명현 한국원예학회 2011 Horticulture, Environment, and Biotechnology Vol.52 No.6
We investigated the role of a dehydroascorbate reductase (SlHDAR1) gene from Solanum lycopersicum leaves in response to abiotic stresses, and the effect of the gene was assessed in transgenic Escherichia coli against oxidative stress. Coding sequence of SlDHAR1 was subcloned into pET-28a(+) expression vector, and transformed it into E. coli BL21 (DE3). The SlDHAR1 gene was induced in tomato leaves in response to cold and wounding, not to absicic acid (ABA). Effective induction of SlDHAR1 protein was observed in E. coli by Isopropyl-β-D-thiogalactopyranoside (IPTG). Increased mRNA level was noted in the E. coli cells transformed with SlDHAR1. The growth of the transgenic E. coli cells expressing SlDHAR1was evidenced to be faster than control strain in the both solid and liquid growth media supplemented with H_2O_2. Taken together, the SlDHAR1 gene could play a role in cold and wound stress responses of tomato plants and the gene may be protective to E. coli to oxidative stress.
Mohammad Humayun Kabir,왕명현 한국응용생명화학회 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.6
Dehydroascorbate reductase (DHAR) plays an important role in the ascorbate-glutathione cycle in plants by controlling the homeostasis of reactive oxygen species. Expression profiles of two DHAR genes (SlDHAR1 and SlDHAR2) in tomato leaves were analyzed in response to abiotic and oxidative stresses as well as their expression in various organs were investigated under normal growth conditions. Multiple copies of SlDHAR homologues exist in the tomato genome. SlDHAR1 expression was dominant in stems and flowers but moderate in leaves; however, SlDHAR2 induction was strong in stems, leaves, and flowers, and young leaves showed the highest expression of SlDHAR2. Both genes were transcribed after treatment with salt, dehydration, and methyl viologen. Prolonged stress reduced SlDHAR genes expression. The level of hydrogen peroxide increased, and DHAR activity was altered by stress treatment. Results indicate that SlDHAR genes could play a role in multiple stress responses.
Suh, Eui Jin,Kabir, Mohammad Humayun,Kang, Un-Beom,Lee, Jong Won,Yu, Jonghan,Noh, Dong-Young,Lee, Cheolju Korean Society for Biochemistry and Molecular Bion 2012 Experimental and molecular medicine Vol.44 No.1
Breast cancer is the most common cancer in women worldwide. It is necessary to identify biomarkers for early detection, to make accurate prognoses, and to monitor for any recurrence of the cancer. In order to identify potential breast cancer biomarkers, we analyzed the plasma samples of women diagnosed with breast cancer and age-matched normal healthy women by mTRAQ-based stable isotope-labeling mass spectrometry. We identified and quantified 204 proteins including thrombospondin-1 (THBS1) and bromodomain and WD repeat-containing protein 3 (BRWD3) which were increased by more than 5-fold in breast cancer plasma. The plasma levels of the two proteins were evaluated by Western blot assay to confirm for their diagnostic value as serum markers. A 1.8-fold increase in BRWD3 was observed while comparing the plasma levels of breast cancer patients (n = 54) with age-matched normal healthy controls (n = 30), and the area under the receiver operating characteristic curve (AUC) was 0.917. THBS1 was detected in pooled breast cancer plasma at the ratio similar to mTRAQ ratio (>5-fold). The AUC value for THBS1 was 0.875. The increase of THBS1 was more prominent in estrogen receptor negative and progesterone receptor negative patients than receptor-positive patients. Our results are evidence of the diagnostic value of THBS1 in detecting breast cancer. Based on our findings, we suggest a proteomic method for protein identification and quantification lead to effective biomarker discovery.
( Myeong Hyeon Wang ),( Mohammad Humayun Kabir ) 한국응용생명화학회 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.6
Dehydroascorbate reductase (DHAR) plays an important role in the ascorbate-glutathione cycle in plants by controlling the homeostasis of reactive oxygen species. Expression profiles of two DHAR genes (SlDHAR1 and SlDHAR2) in tomato leaves were analyzed in response to abiotic and oxidative stresses as well as their expression in various organs were investigated under normal growth conditions. Multiple copies of SlDHAR homologues exist in the tomato genome. SlDHAR1 expression was dominant in stems and flowers but moderate in leaves; however, SlDHAR2 induction was strong in stems, leaves, and flowers, and young leaves showed the highest expression of SlDHAR2. Both genes were transcribed after treatment with salt, dehydration, and methyl viologen. Prolonged stress reduced SlDHAR genes expression. The level of hydrogen peroxide increased, and DHAR activity was altered by stress treatment. Results indicate that SlDHAR genes could play a role in multiple stress responses.
Eui Jin Suh,Mohammad Humayun Kabir,Un-Beom Kang,Jong Won Lee,Jonghan Yu,노동영,이철주 생화학분자생물학회 2012 Experimental and molecular medicine Vol.44 No.1
Breast cancer is the most common cancer in women worldwide. It is necessary to identify biomarkers for early detection, to make accurate prognoses, and to monitor for any recurrence of the cancer. In order to identify potential breast cancer biomarkers, we analyzed the plasma samples of women diagnosed with breast cancer and age-matched normal healthy women by mTRAQ-based stable isotope-labeling mass spectrometry. We identified and quantified 204 proteins including thrombospondin-1 (THBS1) and bromodomain and WD repeat-containing protein 3(BRWD3) which were increased by more than 5-fold in breast cancer plasma. The plasma levels of the two proteins were evaluated by Western blot assay to confirm for their diagnostic value as serum markers. A 1.8-fold increase in BRWD3 was observed while comparing the plasma levels of breast cancer patients (n = 54) with age-matched normal healthy controls (n = 30), and the area under the receiver operating characteristic curve (AUC) was 0.917. THBS1 was detected in pooled breast cancer plasma at the ratio similar to mTRAQ ratio (>5-fold). The AUC value for THBS1 was 0.875. The increase of THBS1 was more prominent in estrogen receptor negative and progesterone receptor negative patients than receptor-positive patients. Our results are evidence of the diagnostic value of THBS1 in detecting breast cancer. Based on our findings, we suggest a proteomic method for protein identification and quantification lead to effective biomarker discovery.