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Affinity Maturation of an Anti-Hepatitis B Virus PreS1 Humanized Antibody by Phage Display
Yang, Gi-Hyeok,Yoon, Sun-Ok,Jang, Myung-Hee,Hong, Hyo-Jeong The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.6
In a previous study we generated an anti-Hepatitis B Virus (HBV) preS1 humanized antibody (HzKR127) that showed in vivo HBV-neutralizing activity in chimpanzees. However, the antigen-binding affinity of the humanized antibody may not be sufficient for clinical use and thus affinity maturation is required for better therapeutic efficacy. In this study, phage display technique was employed to increase the affinity of HzKR127. All six amino acid residues (Glu95-Tyr96-Asp97-Glu98-Ala99-Tyr100) in the heavy (H) chain complementary-determining region 3 (HCDR3) of HzKR127 were randomized and phage-displayed single chain Fv (scFv) library was constructed. After three rounds of panning, 12 different clones exhibiting higher antigen-binding activity than the wild type ScFv were selected and their antigen-binding specificity for the preS1 confirmed. Subsequently, five ScFv clones were converted to whole IgG and subjected to affinity determination. The results showed that two clones (B3 and A19) exhibited an approximately 6 fold higher affinities than that of HzKR127. The affinity-matured humanized antibodies may be useful in anti-HBV immunotherapy.
Outbreaks of Imipenem-Resistant Acinetobacter baumannii Producing Carbapenemases in Korea
Jeong Seok-Hoon,Bae Il-Kwon,Park Kwang-Ok,An Young-Jun,Sohn Seung-Ghyu,Jang Seon-Ju,Sung Kwang-Hoon,Yang Ki-Suk,Lee Kyung-Won,Young Dong-Eun,Lee Sang-Hee The Microbiological Society of Korea 2006 The journal of microbiology Vol.44 No.4
Among 53 Acinetobacter baumannii isolates collected in 2004, nine imipenem-resistant isolates were obtained from clinical specimens taken from patients hospitalized in Busan, Korea. Nine carbapenemase-producing isolates were further investigated in order to determine the mechanisms underlying resistance. These isolates were then analyzed via antibiotic susceptibility testing, microbiological tests of carbapenemase activity, pI determination, transconjugation test, enterobacterial repetitive consensus (ERIC)-PCR, and DNA sequencing. One outbreak involved seven cases of infection by A. baumannii producing OXA-23 ${\beta}-lactamase$, and was found to have been caused by a single ERIC-PCR clone. During the study period, the other outbreak involved two cases of infection by A. baumannii producing IMP-1 ${\beta}-lactamase$. The two clones, one from each of the outbreaks, were characterized via a modified cloverleaf synergy test and an EDTA-disk synergy test. The isoelectric focusing of the crude bacterial extracts detected nitrocefin-positive bands with pI values of 6.65 (OXA-23) and 9.0 (IMP-1). The PCR amplification and characterization of the amplicons via direct sequencing showed that the clonal isolates harbored $bla_{IMP-1}$ or $bla_{oxA-23}$ determinants. The two clones were characterized by a multidrug resistance phenotype that remained unaltered throughout the outbreak. This resistance encompassed penicillins, extended-spectrum cephalosporins, carbapenems, monobactams, and aminoglycosides. These results appear to show that the imipenem resistance observed among nine Korean A. baumannii isolates could be attributed to the spread of an IMP-lor OXA-23-producing clone. Our microbiological test of carbapenemase activity is a simple method for the screening of clinical isolates producing class D carbapenemase and/or class B $metallo-{\beta}-lactamase$, in order both to determine their clinical impact and to prevent further spread.
Fabrication of a Thermopneumatic Valveless Micropump with Multi-Stacked PDMS Layers
Jeong, Ok-Chan,Jeong, Dae-Jung,Yang, Sang-Sik The Korean Institute of Electrical Engineers 2004 KIEE International Transactions on Electrophysics Vol.4C No.4
In this paper, a thermopneumatic PMDS (polydimethlysiloxane) micropump with nozzle/diffuser elements is presented. The micropump is composed of nozzle/diffuser elements as dynamic valves, an actuator consisting of a circular PDMS diaphragm and a Cr/Au heater on a glass substrate. Four PDMS layers are used for fabrication of an actuator chamber, actuator diaphragm by a spin coating process, spacer layer, and nozzle/diffuser by the SU-8 molding process. The radius and thickness of the actuator diaphragm is 2 mm and 30 ${\mu}{\textrm}{m}$, respectively. The length and the conical angle of the nozzle/diffuser elements are 3.5 mm and 20$^{\circ}$, respectively. The actuator diaphragm is driven by the air cavity pressure variation caused by ohmic heating and natural cooling. The flow rate of the micropump in the frequency domain is measured for various duty cycles of the square wave input voltage. When the square wave input voltage of 5 V DC is applied to the heater, the maximum flow rate of the micropump is 44.6 ${mu}ell$/min at 100 Hz with a duty ratio of 80% under the zero pressure difference.
Fabrication and Drive Test of a Peristaltic Thermopnumatic PDMS Micropump
Ok Chan Jeong,Sin Wook Park,Sang Sik Yang 대한기계학회 2005 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.19 No.2
This paper presents fabrication and drive test of a peristaltic PDMS micropump actuated by the thermopneumatic force The micropump consists of the three peristaltic-type actuator chambers with microheaters on the glass substrate and a microchannel connecting the chambers and the inlet/outlet port The micropump is fabricated by the spill-coating process, the two-step curing process, the JSR (negative PR) molding process, and etc The diameter and the thickness of the actuator diaphragm are 2 5 mm and 30 ㎛, respectively The meniscus motion in the capillary tube is observed with a video camera and the flow rate of the micro pump is calculated through the frame analysis of the recorded video data. The maximum flow rate of the micro-pump is about 0.36 μL/ sec at 2 Hz for the zero hydraulic pressure difference when the 3-phase input voltage is 20 V.
Yang, Moon-Hee,Yoo, Kyung-Hyun,Yook, Yeon-Joo,Park, Eun-Young,Jeon, Jeong-Ok,Choi, Seo-Hee,Park, So-Young,Woo, Yu-Mi,Lee, Min-Joo,Park, Jong-Hoon Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.2
PCD (programmed cell death) is important mechanism for development, homeostasis and disease. To analyze the gene expression pattern in brain cells undergoing PCD in response to serum deprivation, we analyzed the cDNA microarray consisting of 2,300 genes and 7 housekeeping genes of cortical cells derived from mouse embryonic brain. Cortical cells were induced apoptosis by serum deprivation for 8 hours. We identified 69 up-regulated genes and 21 down-regulated genes in apoptotic cells. Based on the cDNA microarray data four genes were selected and analyzed by RT-PCR and northern blotting. To characterize the role of UNC-51-like kinase (ULK2) gene in PCD, we investigated cell death effect by ULK2. And we examined expression of several genes that related with PCD. Especially GAPDH was increased by ULK2. Theses findings indicated that ULK2 is involved in apoptosis through p53 pathway.
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( Jeong Ok Yang ),( Jook Sook Lee ) 한국운동역학회 2021 한국운동역학회지 Vol.31 No.4
Objective: The aim of this study was to analyze domestic and foreign studies using virtual reality or metaverse for exercise rehabilitation in order to help the disabled or elderly patients with exercise rehabilitation, and suggest a method for using metaverse for exercise rehabilitation. Method: In this study, after analyzing and discussing various information related to the metaverse and exercise rehabilitation through electronic search of recently published papers, academic journals, books, and internet websites, the exercise rehabilitation plan using the metaverse was proposed. Results: In the case of domestic research, the diversity of virtual reality application technology for the rehabilitation of disabled and elderly patients was not secured, but recently, virtual reality or metaverserelated technologies were developed and specialized in a form suitable for exercise rehabilitation. In the case of overseas studies, it was analyzed that exercise rehabilitation using virtual reality and metaverse games for the rehabilitation of disabled and elderly patients can help improve brain, physical ability, and anti-aging by activating the body and mind. Conclusion: Smart metaverse health care is actively introduced to exercise rehabilitation, metaverse telemedicine business is applied to exercise rehabilitation programs, and digital twin games and exercise rehabilitation programs developed by metaverse related companies take into account the characteristics of disabled and elderly patients. If customized smart metaverse healthcare is used for exercise rehabilitation, it is analyzed that it can lead this field.
Development of Cultural Enterprise in the Marine City Busan by Hosting Mega Sports Events
Jeong-Ok Yang,Keun-Mo Lee,Kuk-Wong Seo,Yun-Ae Jeon 한국체육학회 2008 International journal of human movement science Vol.2 No.2
The purpose of this was to analyze how the international events hosted so far affected the development of local society through economic, cultural, and social ripple effects and how infrastructure of facilities and human capitals as the result of hosting sports events affected sports culture. Also developmental strategies to ensure local identity and host mega sports events for local economy and cultural development was discussed in the light of growing cultural enterprise in the marine city Busan. Busan city decided to invite the Olympic Games in 2020 as a part of Visions and Strategies for Busan Development 2020 and planned to submit the application for candidate cities to the government and KOC until November. If the Olympics is invited, the direct ripple effect on the national economy is estimated 20 trillion 290 billion won and the number of jobs created will be 115 thousand. According to the result from the feasibility study of hosting the Olympic Games in 2020, the direct ripple effects was estimated 12 trillion 743.8 billion won of production, 115 thousand of jobs, 2 trillion 329.2 billion won of incomes, and 5 trillion 232.2 billion won of added value. Hosting mega sports events is the communication media with strong ripple effects like economic effects, industrial development, regional development, international exchange and the activation of culture and art. Also it can create own local color through the forming local identity. The local cultural assets created can give history and pride to its people and also have the function to transfer local value industrially.