엉겅퀴로부터 분리한 Silymarin 및 Silybin이 Macrophages에 의한 사람 Low Density Lipoprotein의 산화에 대한 항산화 효과

류병호,이백천,정영기 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1

This study was undertaken to evaluate an antioxidative activity of silymarin and silybin obtained from Silybum marianum against oxidation of human low density lipoprotein (LDL). The electrophoretic mobility observed apparently was higher phase for LDL oxidized by macrophages compared to native LDL. Silymarin and silybin inhibited the copper-catalysed oxidation of human LDL in a dose-dependent manner. Silymarin and silybin at the concentration of 50μM/ml also inhibited the copper catalysed oxidation of LDL induced by the cell J774 and macrophages. LDL reisolated from the cell incubation in the presence of silymarin or silybin was degraded at rates similar with native LDL. Silymarin or silybin found to be potential inhibitors against oxidation of ^125I-LDL by macrophages and endothelial cells.

이담자균 효모 Rhodosporidium toruloides에서 Rhodotorucine A에 의한 막단백질 인산화의 저해와 Trigger Peptidase의 관련성

정영기,이태호,류병호 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

이담자 효모균 Rhodosporidium toruloides 접합형 a 세포의 막단백질 가용화 분획을 비변성 전기영동에 의하여 인산화 한 결과 인산화 되는 단백질을 발견하였다. 이 단백질의 인산화는 Rhodotorucine A (Rh. A)에 의하여 저해되었다. 본 인산화 단백질에는 trigger-peptidase (TPase)가 존재하고 있는 것을 확인 하였으며, TPase inhibitor(antipain)를 반응액에 첨가할 경우 Rh. A에 의한 인산화 저해작용은 나타나지 않았다. 그리고, Rh. A를 trypsin으로 분해한 산물을 반응액에 첨가했을 경우 인산화 저해현상이 있는 것으로 보아, 인산화의 저해반응에는 TPase가 중요한 작용을 하는 것이 확인되었다. [γ-^32P]ATP was used to test phosphorylation of membrane proteins of mating type a cells of heterobasidiomycetous yeast Rhodosporidium toruloides separated by non-denaturing electrophoresis. The phosphoprotein was observed in the membrane proteins. The phosphorylation was inhibited by the pheromone rhodotorucine A (Rh. A) secreted by mating type A of the yeast. Rh. A didn't inhibit the phosphorylation in the presence of a trigger peptidase (TPase) inhibitor, antipain. Partially digested Rh. A by trypsin maintained the phosphorylation inhibitory activity. These results show that TPase activity plays an important role in the transduction of pheromone signal in the yeast.

이담자균 효모 Rhodosporidium toruloides에서 Rhodotorucine A에 의한 막단백질 인산화의 저해와 Trigger Peptidase의 관련성

정영기,이태호,류병호 동의대학교 기초과학연구소 1997 基礎科學硏究論文集 Vol.7 No.1

[γ-^32P]ATP was used to test phosphorylation of membrane proteins of mating type a cells of heterobasidiomycetous yeast Rhodosporidium toruloides separated by non-denaturing electrophoresis. The phosphoprotein was observed in the membrane proteins. The phosphorylation was inhibited by the pheromone rhodotorucine A (Rh. A) secreted by mating type A of the yeast. Rh. A didn't inhibit the phosphorylation in the presence of a trigger peptidase (TPase) inhibitor, antipain. Partially digested Rh. A by trypsin maintained the phosphorylation inhibitory activity. These results show that TPase activity plays an important role in the transduction of pheromone signal in the yeast.

다축 제어 시뮬레이터 링크부의 동특성에 관한 연구

정상화,류신호,박용래 朝鮮大學校 機械技術硏究所 2001 機械技術硏究 Vol.4 No.1

차량개발과정에 있어서 완성차량에 대한 실험방법은 크게 실차 주행실험과 실험실 재현실험의 두가지로 분류할 수 있다. 이 중 실험실 재현실험은 주어진 실험실내의 공간에서 차량의 주행상태를 반복, 재현함으로써 시간의 절감과 함께 공간 및 환경의 제약에서 벗어날 수 있으며, 실험의 지속적 재현성과 실험중인 차량을 수시로 관찰, 분석할 수 있어 취약부분 및 성능의 조기 확인이 가능한 장점이 있다. 이런 이유로 많은 국외 자동차사에서는 다축 제어 시뮬레이터를 이용한 실험실 재현실험을 활성화하여 차량개발기간의 단축은 물론 높은 신뢰성을 가진 고품질 자동차의 개발에 활용하고 있는 실정이나, 아직까지 국내에서는 수입하여 활용측면에서는 아직 초보단계를 벗어나지 못하고 있다. 본 논문에서는 실험실 재현실험의 구동부인 다축 제어 시뮬레이터에 작용하는 4가지 요소중 3가지 요소인 연직·전후·횡 하중등을 복합적으로 재현할 수 있는 링크 구조를 설계하여 동역학 해석 프로그램인 ADAMS를 이용하여 시뮬레이션을 수행하고, 링크부의 안정성 평가 및 최적모델의 사양을 결정하기 위해 FEM 해석을 하였다. Test in the development of vehicle consist of driving test and simulation test. The last one has many advantages. It can reduse time and cost during development, can overcome the spacial and environmental limitation, and can provides repeatabilities of similar experiments and various data. In these reason, the simulation test is widely used in the developed countries for developing new vehicles and analyzing the dynamic characteristics of vehicle developed.

해양미생물로부터 카로테노이드 색소의 생산을 위한 최적조건

정영기,박정욱,주우홍,류병호,정명주,김태수 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1

The optimal medium composition for the production of carotenoid pigment from Haloarcular sp. EH-1 as a dietary for fishes were 1.0% sucrose, 1.0% yeast extract, 25% sodium chloride, 0.3% sodium citrate, 0.2% potassium chloride, 2.0% magnesium sulphate, 0.002% ferric sulphate(pH7.0). The incubation temperate, aeration rate and agitation speed were 40℃, 100ml medium/500ml vol. shaking flask, and 180 rpm/min. The carotenoid pigment production was highest after 5 days of incubation with the light.

채엽시기에 따른 한국산 녹차의 성분비교 연구

류화정,고영수 漢陽大學校 韓國生活科學硏究所 1995 韓國 生活 科學 硏究 Vol.- No.13

Chemical constituents of Korean green tea which were harvested at four different times(May, July, August, September) were studied. The Contents of chemical constituents varied according to their harvested times as follows. 1.Water(2.4%, 2.8%, 2.2%, 1.9%) Waterextract(35%, 34%, 37%, 34%), Carbon(28.6%, 28.9%, 29.5%, 49.0%), Hydrogen(6.8%, 6.5%, 6.6%, 6.3%), Total Nitrogen(5.98%, 4.12%, 3.21%, 3.08%), Oxygen(32.3%, 33.1%, 32.6%, 33.0%) 2.Crude fiber(7.2%, 8.3%, 10.2%, 11.2%), Crude fat(1.38%, 1.45%, 2.52%, 2.15%), Crude protein(31.3%, 20.4%, 17.1%, 16.9%) 3.Total sugar(5.5%, 7.1%, 10.6%, 11.8%), Reducing sugar(0.21%, 2.52%, 2.50%), Sucrose(0.45%, 2.86%, 5.42%, 6.32%), Fructose(0.25%, 0.12%, 0.09%, 0.03%), Glucose (1.62%, 1.02%, 0.54%, 0.45%) 4.Tannin(11.2%, 15.1%, 13.1%, 12.3%), Theaflavin(0.007%, 0.09%, 0.06%, 0.056%) 5.Catechin was conposed of GA(0.17%, 022%, 019%, 0.18%), (-)-EGC (2.71%, 3.21%, 3.01%, 2.8%), (-)-CA(0.15%, 0.16%, 0.16%, 0.17%), (-)-EC(1.21%, 1.34%, 1.36%, 1.3%), -EGCG(6.81%, 8.21%, 7.45%, 7.24%), (-)-ECG(1.41%, 1.91%, 1.62%, 1.50%) 6.Amino acids(1.90%, 1.07%, 0.99%. 0.72%), Caffeine(3.12%, 2.96%, 2.01%, 1.48%)

방사선조사에 따른 백서 악하선내 혈관 내피세표 미세구조의 조기변화

손정익,최갑식,류정수,배용철 大韓口腔顎顔面 放射線學會 1995 Imaging Science in Dentistry Vol.25 No.1

The purpose of this study was to investigate the early irradiation changes on the ultrastructure of the capillary endothelial cell in the rat submandibular glands. For the study, 110 Sprague-Dawley strain male rats were singly irradiated to their neck regions with the doses of 2Gy, 5Gy, and 10Gy by 6MV X-irradiation, and sacrificed on the 3 hours, 6 hours, 12 hours, 1 day, 3 days, 7 days, and 14 days after irradiation. The authors observed the histologic and ultrastructural changes of the capillary endothelial cell using the light and electron microscopes. The results were as follows: 1.In the light microscopic examination, the capillary dilation was observed on the 6 hours group and the capillary density was slightly increased on the 12 hours group after 2Gy and 5Gy irradiation. And luminal size and capillary density were decreased on the 3 days and the 7 day groups after irradiation, after then, they were recovered. But capillary density was still decreased on the 14 days group after 10Gy irradiation. 2.In the transmission electron microscopic examination, the mild proliferation of cytoplasmic process of the endothelial cell and reduction in luminal size were observed on the 3 hours group after irradiation. After then, endothelial swelling, marked proliferation of cytoplasmic process, thickened basal lamina, and numerous pinocytotic vesicles were observed after the 1 day group after irradiation. Thickened basal lamina and numerous pinocytotic vesicles were still observed until the 7 days group after irradiation. These changes were recovered to normal on the 14 days group after 2Gy and 5Gy irradiation, but not after 10Gy irradiation. 3.In the scanning electron microscopic examination, the dilation of conduits and constriction, and meandering were observed on the 1 day group after 10Gy irradiation. These changes were observed with increased coarseness of the surface of the vascular resin casting on the 3 days group after irradiation. 4.From the above results, endothelial swelling, proliferation of cytoplasmic process, and thickening of the basal lamina appeared before the 6 hours group after irradiation. And these changes may also induce the increase of the capillary number and luminal size, after then, capillary permeability was increased via the increase of the number of pinocytotic vesicles. The changes were observed earlier and more apparent with the increase of the irradiation doses under the dose of 10Gy irradiation.

불량행위 청소년의 가족 폭행에 관한 사회 정신의학적 고찰(Ⅳ)

박범룡,류영호,조동환,김정기 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.3

연구목적: 이 연구는 청소년의 가족 폭행에 대하여 조사하고 가족폭행의 관점에서 불량행위 청소년의 특성을 규명하고자 하였다. 방 법: 저자들은 불량행위 청소년 238명, 범죄 청소년 209명, 그리고 부산시대 고등학교 학생 230명을 대상으로 설문지, 다면적 인성 검사(Minnesota Multiphasic Personality Inventory), 그리고 한국판 부모-자녀 결합도 검사(Parental Bonding Instrument0 등의 도구를 사용하여 조사하였고, 그것으로 세집단을 Chi-square test, T-test, 그리고 ANOVA를 사용하여 비교 분석하였다. 결 과: 대개의 경우 불량행위 청소년의 사회경제적인 상태가 학생군보다 더 불량하였고, 범죄군보다는 더 양호하였다.(p<.05) 일반적으로 폭행가해자들은 손위형제였고, 신체적으로도 피해자보다 더 강했고, 형제간의 우애는 좋지 않았다. 학생군과 비교할 때 존속에 대한 폭행을 포함한 폭행의 심각성은 불량행위 청소년과 범죄 청소년에서 존재하고 있었다. 다면적 인성검사와 부모-자녀 결합도 검사의 결과, 가족폭행군과 비폭행군을 비교할 때, 불량행위 청소년군의 경우에는 Sc척도와 Passive aggresive index에서 학생군의 경우에는 아버지 과보호에서 의미있는 차이를 보였지만 그외의 척도에서는 의미있는 차이를 찾을 수가 없었다. 결 론: 일반적으로 가족폭행의 특성으로도 기존의 사회 정신의학적 고찰의 결과와 같이 불량행위 청소년군이 학생군과 범죄청소년군의 중간특성을 가지고 있는 것으로 나타났으며 가족 폭행 문제의 심화를 막기 위하여 이 집단에 대한 적절한 개입이 필요할 것으로 생각되어진다. Objectives : This research was performed to identify family violence by male adolescents, and to examine the characteristics of male adolescent delinquents in terms of domestic violence. Methods : We surveyed 677 male adolescents including 238 delinquents from BBS(Big brothers and sisters) in Pusan, 209 criminal adolescents from adolescent correctional institution in Pusan, 230 high school students through questionnaire, MMPI(Minnesota Multiphasic Personality Inventory), and PBI(Parental Bonding Instrument), and then compared these three groups by Chi-square test, T-test, and Anova. Results : In most cases, male delinquent adolescents were placed in poorer socio-economic conditions than those of students, but in better socio-economic conditions than those of criminal adolescents. In general, the assaulters were senior family members, physically stronger than sufferers, and have bad brotherliness. Compared with normal students, the serious problem of violence including violence to the ancestors in delinquent and criminal adolescents. On results of MMPI and PBI test, there were significant difference between violent group and non-violent group on the Sc score and Passive aggressive index in adolescent delinquents and parental overprotection in normal students. Conclusion : Generally the characteristics of family violence by male delinquent adolescents were intermediate between those of criminal adolescent and students, these results were same as those of previous socio-psychiatric study, and so the intervention in criminal adolescents is needed for the prevention of family violence.

Dimethomorph의 제형에 따른 고추 역병 방제 효과

박정용,류연주,류나현,이용세 대구대학교 생명과학연구소 2004 생명과학연구 Vol.3 No.1

Dimethomorph(DMM) is an effective Oomycete fungicide useful for the control of late blight of potato and pepper by preventative sprays. Efficacy of five formulations, suspension concentrate (DM150), wettable powder (DM250) and three different dispersible concentrates (DM630, DM631, DM632), were investigated in their biological activity against phytophthora blight of red-pepper in vitro and in vivo. Their in vitro biological activities against mycelial growth, zoosporangial formation and zoosporangial germination of Phytophthora capsici were similar. The incidence of late blight on red pepper plants was progressively increased from June 11 to August 8 in untreated plants. However, in the case of DMM treated plants diseased plants, were increased after July 11. When compared with that on July 11, the incidence of late blight on July 18 in the treated plants except in DM630 were increased drastically. This increase were thought to be the result of flooding with more than 80 mm rainfall at July 15. The incidence of late blight on July 29 in the treated plants were increased compared with that on July 18. These also thought to be the result of flooding with more than 100 mm rainfall on July 23. All of the tested DMM formulations showed similar high control value against the late phytophthora blight on red pepper plants in the field test until July 11. However, after flooding the test field, DM630 showed the greatest disease control efficacy. DM250 showed relatively poor disease control efficacy in the test. Therefore more studies on the DM630 formulation are needed.

참외 흰가루병에 대한 살균제의 예방 및 치료효과 검정

장태현,류연주,임혜정,임수진,최미영,정병룡,김철웅,이용세 한국환경농학회 2006 한국환경농학회지 Vol.25 No.1