Expansin으로 형질전환 된 담배의 생장 특성

신하정, 성현정, 백승준, 김보미, 엄유리, 조은정, 정찬문, 이이 충북대학교 연초연구소 2008 煙草硏究 Vol.21 No.-

-본문참고-

모유 수유아와 조제 분유 영양아에서 수유 방법에 따른 아미노산 농도 비교 및 혈청 트립토판 농도와 수면 유도와의 관계

정다운,김은영,양은석,박상기,박영봉,문경래 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Objective: Sleep behavior is modulated by serotonergic neurons within the brain, and the synthesis and release of serotonin by such neurons is thought to be influenced by the availability of tryptophan, the amino acid precursor of serotonin. Formula-feeding infants have depressed plasma tryptophan concentration relative to breast milk-feeding infants. Because tryptophan alters sleep waking patterns in adults, a study was designed to determine the difference of sleep latency relative to differences in plasma tryptophan and tryptophan : large neutral amino acid (LNAA) ratio between formula-feeding infants and breast milk-feeding infants. Method: 45 newborns who were born in Chosun University Hospital from December 2002 to December 2003 were selected. The newborns were divided to three group, 15 newborns were fed breast milk and 15 newborns were fed formula A, and last 15 newborns were fed formula B. At 6 weeks and 12 weeks, infants were sampled for measuring of serum amino acid level and tryptophan, tryptophan : LNAA ratio. And we taught infants' parents to measure sleep latency that means the time after a feeding to the first episode of active REM sleep that persist ≥ 1 min. Result: 1) At 6weeks, Serum α-amino-n-butyric acid, citrulline, tryptophan level was higher in formula A group than breastmilk group (P<0.05) and proline level was lower than breast milk group (P<0.05). In formula B group, serum n-amino-n-buryric acid, citrulline, cystine, hydroxylysine, hydroxyproline, isoleucine, leucine, lysine, methionine, phenylalanine, taurine, threonine, valine levels were higher than breastmilk group (P<0.05). And Serum isoleucine, methionine, proline, valine level were higher in formula B group than formula A group (P<0.05). 2) At 12 weeks, serum glutamic acid, methionine, ornithine levels were higher in formula group A than breast milk group (P<0.05). Serum β-alanine, aspartic acid, α-amino-n-butyric acid, ethanolamine, glutamic acid, threonine level were higher in formula B group than breast milk group (P<0.05). Serum β-alanine, ethanolamine levels were higher and arginine level was lower in formula B group than formula A (P<0.05). 3) At 6 weeks, serum tryptophan concentration and tryptophan: LNAA ratio were higher in formula A group (P<0.05). Sleep latency was 21 minutes in formula group A, 24 minutes in breast milk group, 25 minutes in formula B group but there was no statistically significance (P>0.05). 4) At 12 weeks, serum tryptophan concentration and tryptophan: LNAA ratio, time after feeding to the first episode of active REM sleep were no difference with each other group. Conclusion: There is significant difference of serum amino acids between breast-feeding infants and formula-feeding infants. And serum tryptophan and tryptophan: LNAA ratio differ between brest-feeding infants and formula-feeding infants. But there is no association between serum tryptophan, tryptophan: LNM ratio and sleep latency.

비병원성 야생효모 Rhodosporidium toruloides HGG35-2와 호중구 elastase 저해제 생성효모인 Wickerhamomyces anomalus HO9-2로부터 Killer toxin 생산

문정수, 장지은, 김하근, 이종수 배재대학교 자연과학연구소 2023 自然科學論文集 Vol.34 No.1

항진균 바이오의약의 소재인 killer toxin을 비병원성 야생효모로부터 생산하기 위하여 25개 비병원성 색소 생성 효모들의 killer toxin 활성을 측정하여 우수균주로 Rhodosporidium toruloides HGG35-2와 Wickerhamomyces anomalus HO9-2을 선발하였다. 이들 killer toxin 생성균주들의 효모 감수성 균주들에 대하여 killer 활성을 측정한 결과 시판 포도주등의 알콜발효로 이용되고 있는 Saccharomyces cerevisiae (La Parisienne®)에 대하여 우수한 항진균활성을 보였다. Killer toxin production of non-pathogenic wild yeasts, Rhodosporidium toruloides HGG35-2 and Wickerhamomyces anomalus HO9-2 were investigated. Rhodosporidium toruloides HGG35-2 showed strong killer activity against pathogenic yeast, Candida guilliermondii AHP28-1 and commercial alcohol fermentation yeast, Saccharomyces cerevisiae(La Parisienne). Wickerhamomyces anomalus HO9-2 also showed killer activity against Rhodotorula nothofagi JSC10-1 and commercial alcohol fermentation yeast, Saccharomyces cerevisiae(La Parisienne®).

탈지구자기장이 초파리 집단의 선택불리에 미치는 영향

정승문,박은규 圓光大學校 基礎自然科學硏究所 2001 基礎科學硏究誌 Vol.19 No.1

본 연구에서는 전북 김제시 청하의 자연집단 야생형 초파리와 성연관 골연변이형 호아색체-백색안 초파리를 Helmholtz coils 장치를 사용해서 유도한 탈지구자기장내 초파리 집단과 비교집단인 지구자기장내 초파리 집단으로 사육해서 발생된 성적 선택빈도를 조사하여, 선택불리에 관한 다음과 같은 결과를 얻었다. 야생형 유전자를 운반한 염색체의 선태빈도는 25세대까지 전체 평균빈도가 위의 두 집단에서 각각 0.7284,0.7360으로 관찰되었다. 이 결과는 탈지구자기장내 초파리 집단에서 더 강한 선택불리를 보여 주었다. 성연과 돌연변이형 yω 유전자를 운반한 염색체의 선택빈도는 위의 각각의 조절된 자기장내의 초파리 집단에서 각각0.0256, 0.0191로 관찰되었다. 이 결과는 탈지구자기장내 초파리 집단보다 지구자기장내 초파리 집단에서 더 강한 선택불리 행동을 보여 주었다. 초파리 집단의 전체 평균 발생율은 위의 초파리 집단에서 각각 32.10%, 67.90%로 관찰 되었다. 이 결과는 지구자기장내 초파리 집단의 발생율이 상대적으로 높은 수치로 조사되었다. Duncan's multiple range test와 LSD 값으로 유의 차이를 검정하였던 바 야생형 그룹과 다른 3개의 돌연변이형 그룹 사이에서 고도의 유의 차이가 있음이 밝혀졌다. To elucidate the genetic behavior of selective disadvantage among sex-linked mutants of Drosophila melanogaster vred and kept by the genetic study laboratory room, Wonkwang University and wild type collected at Chongha, Kimje city, Chollabuk-do, and reared under the in fluence of two different strength of magnetic field, this study i.e., the without magnetic field generated by Helmholtz coils and the ambient magnetic field, this study was carried out at the above indicated laboratory room. The mean selective frequencies of wild type gene-carrying chromosomes up to twenty-fifth generation, affected by two different magnetic fields, showed 0.7284 and 0.7360 with the magnetic field strength as mentioned other before. The data explains that Drosophila melanogaster population reared in the without magnetic field showed stronger selective disadvantage. The selective frequencies of the individual of yω gene-bearing chromosomes showed 0.0256 and 0.0191 by the same order of magnetic field strength indicate above. It was shown that the fly population reared at the ambient magnetic field cleared the relatively stronger selective disadvantage. The over-all mean emergence rates of Drosophila melanogaster populations reared in controlled magnetic fields indicated above showed 32.10% and 67.90% respectively higher emergence rate than the without magnetic field population. Through Duncan's multiple range test and LSD value, the highly significant differences between the wild type and anyone of the other mutants were calculated.

일반 여성과 영양사의 공중화장실 이용 후 손 씻기 실태

문정아,유창희,이경은 서울여자대학교 자연과학연구소 2011 자연과학연구논문집 Vol.23 No.-

The purposes of this study were to examine handwashing practices among dietitians and female adults in public restrooms and to determine environmental factors affecting handwashing. Trained investigators observed 312 women (156 dietitians, 156 female adults) handwashing in 54 public restrooms in Seoul and recorded facilities of the restrooms. The data were collected during May and July, 2009 and analyzed by t-test and χ2-test using SPSS 14.0. More dietitians (91.7%) washed their hands after using public restrooms than the female adults (78.8%) (p<.01). However, more than half of the subjects washed hands without soaps. The dietitians (13.1 seconds) washed hands longer than the female adults (10.1 seconds) (p<.01), but both groups washed hands shorter than 20 seconds, which is recommended for proper handwashing. Both warm and cold water was available at 63% of the observed public restrooms. Significantly more females washed their hands at the restrooms where both warm and cold water was available (88.5%) than where only cold water was available (80%)(p<.05). Literature has revealed that most consumers understand the importance of handwashing. However the finding of the study showed that their handwashing practices in everyday life did not meet their perception. This observation study disclosed the gap which was not reported from the studies conducted based on surveys. From now on, messages for the consumers should focus on when and how their hands should be washed. Finally dietitians should be a role model of proper handwashing in foodservice facilities as well as in everyday life. Key words:handwashing, observation, dietitians, female adults, public restooms.

한국 초파리 집단의 선택불리에 관한 지구 자기장이 소거된 자기장 영향

정승문,최오옥,박은규 圓光大學校 1996 論文集 Vol.32 No.2

본 연구에서는 한국 남해안 거제도의 자연집단의 야생형 초파리와 성연관 돌연변이형 황색체-백색안 초파리를 Helmholtz coils 장치를 사용해서 유도한 지구 자기장이 소거된 초파리집단과 비교집단인 지구 자기장내의 초파리 집단으로 사육해서 발생된 성적 선택빈도를 조사하여, 선택불리에 관한 다음과 같은 결과를 얻었다. 야생형 유전자를 운반한 염색체의 선택빈도는 20세대까지 전체 평균 빈도가 위의 2집단에서 각각 0.7212, 0.7180으로 관찰되었다. 이 결과는 지구 자기장이 소거된 집단에서 더 약한 선택분리 행동을 보여주었다. 성연관 돌연변이형 yw 유전자를 운반한 염색체의 선택빈도는 위의 각각의 조절된 자기장내의 초파리 집단에서 각각 0.0306, 0.0372로 관찰되었다. 이 결과는 자기장이 소거된 초파리 집단 보다 지구 자기장 내의 초파리 집단에서 더 약한 선택불리 행동을 보여 주었다. 초파리 집단의 전체 평균 발생율은 위의 초파리 집단에서 각각 53.40%, 40.60%로 관찰되었다. 이 결과는 지구 자기장내의 초파리 집단의 발생율이 상대적으로 낮은 수치로 조사되었다. Duncan's multiple range test와 LSD값으로 유의 차이를 검정하였던 바 야생형 그룹과 다른 3개의 돌연변이형 그룹 사이에서 고도의 유의차이가 있음이 밝혀졌다. To elucidate the genetic behavior of selective disadvantage among sex-linked mutants of Drosophila melanogaster bred and kept by the genetic study laboratory room, Wonkwang University and the wild type collected at Keojaedo island, and reared under the influence of two different strength of magnetic fields, i.e. the without magnetic field generated by Helmholtz coils and the ambient magnetic field, this study was carried out at the above indicated laboratory room. The mean selective frequencies of wild type gene-carrying chromosomes up to twentieth generation, affected by two different mafnetic fields, showed 0.7212 and 0.7180 with the magnetic field strength as mentioned other before. The data explains that Drosophila melanogaster population reared in the without magnetic field showed lower selective disadvantage. The selective frequencies of the individual of yw gene-bearing chromosomes showed 0.0306 and 0.0372 by the same order of magnetic field strength indicated above. It was shown that the fly population reared at the ambient magnetic field cleared the relatively lawer selective disadvantage. The over-all mean emergence rates of Drosophila melanogaster populations reared in controlled magnetic fields indicated above showed 53.40% and 46.60% respectively. Referring to these data, the fly population raised in the ambient magnetic field showed a relatively lower emergence rate than the without magnetic field population. Through Duncan's multiple range test and LSD value, the highly significant differences between the wild type and anyone of the other three mutants were calculated.

월경전증후군의 진단분류학적 개념 및 원인과 치료 : Nosology, Etiology, and Treatment

문석우,서정석,류은정,남범우 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

Since the emergence of the term premenstrual syndrome (PMS) in the 1950s, PMS has become an increasingly discussed topic in popular media sources. Thus self management techniques are easily accessed by women through the media or through their peers. Premenstrual dysphoric disorder (PMDD) comprises extremely distressing emotional and behavioral symptoms. Women with PMDD report premenstrual symptoms, primarily mood symptoms, that are severe enough to seriously interfere with their life style and relationships and usually do not respond to conservative and conventional interventions. Although women with PMS are most often seen in primary care or by their obstetricians/gynecologists, the result of recent study suggested a high rate of missed diagnoses. The premenstrual syndromes include the more common PMS, the less prevalent PMDD, and menstrual psychosis. The etiology of PMS and PMDD is still largely unknown. That PMS and PMDD are primarily biological phenomena is underscored by recent, convincing evidence. Therapeutic interventions for PMS and PMDD range from the conservative to treatment with psychotropic medication and, for the more extreme cases, hormonal therapy or surgical procedures.

Helm-Holtz coil裝置 초파리集團의 選擇不利에 관한 遺傳學的 硏究

鄭勝文,朴泰永,朴殷圭 圓光大學校 基礎自然科學硏究所 1990 基礎科學硏究誌 Vol.9 No.2

In order ot elucidate the genetic behavior of sexually selective disadvantage between visible mutants and wild type of Drosophila melanogaster in the Helm-Holtz coil apparatus without the ambient magnetic filed, this study was carried out From the result of the present experiment, the frequencies of all the flies between in the apparatus and .in the ambient field showed that the mutants decreased and the wild type increased according to the process of the generations. The whole average frequencies of the + gene-bearing chromosomes on the selective advantage through initial to twenty five generations in the apparatus and in the field were 72.84%, and 73.60%, respectively. It was observed that the sexually selective disadvantage of the + gene of wild type in the apparatus were higher than 'hat in the field. Each frequency on the sexually selective advantage rates of the yw gene-bearing chromosomes showed as 2.56% in the apparatus and as 1.91% in the field. Thus the selective disadvantage of the farmer was lower thatn that of the latter. The average frequencies of the w and the y gene-bearing chromosomes in the selective advantage rates were nearly the same under both conditions of the apparatus and the field showing 0.07%, 0.08% and 0.03%, 0.03%, respectively. The percentage of progeny due to eggs Lying by mated females on a complete food in the apparatus and in the field was found that each frequency in the whole average frequencies revealed 35.23% in the apparatus and 64.77% in the field. This suggested that the frequencies of all the flies in the apparatus decreased to nearly half of that of them in the field. The average frequency of each gene through initial to twenty five generations in the apparatus and in the field was calculated to be 72.84% and 73.60% in + gene. 2.56% and 1.91% in yw gene, 0.07% and 0.08% in w gene, 0.03% and 0.03% in y gene, respectively. From these data, the yellow bodied mutant was the highest in selective disadvantage and weakened in order of wild, yellow-white and white eyed mutant And summarized are that, as to the gene frequencies of wild, yellow-white, white, and yellow of Drosophila melanogaster, strength and weakness of selective disadvantage was varied due to X-linked genes.

한국산 초파리집단의 발생율에 대한 자기장의 영향

정승문,박은규 圓光大學校 基礎自然科學硏究所 2000 基礎科學硏究誌 Vol.18 No.1

거제도에서 채집한 야생형 초파리와 성연관 돌연변이체 사이에서의 성적선택에 따른 자기장의 영향을 조사하기 위하여 본 연구를 수행했다. 본 실험에서의 초파리는 4개의 다른 자기장의 힘의 영향하에서 사육되었다. 즉 지구자기장, 탈지구자기장, 지구자기장 2배의 자기장과 지구장기장 8배의 자기장을 설정하고 이들 제어환경하에서 상기 재료 초파리들의 수정란에서 우화하기까지의 발생율을 조사하여 그 결과를 분석하였다. 전체평균 발생율은 지구자기장내의 초파리 집단, 탈지구자기장내의 초파리 집단, 지구자기장 2배의 초파리 집단, 지구자기장내 8배의 초파리 집단에서 각각 20.35%, 24.55%, 25.27%, 29.83%로 지구자기장내의 초파리 집단 전체평균이 Helmholtzcoils 장치를 이용해 조절된 다른 3개의 초파리 집단에서 보다 상대적으로 낮은 발생율을 보여 주었다. 상이한 자기장내에서의 계수일자에 관한 발생빈도를 보면, 계수 첫째날부터 셋째날까지의 빈도가 지구자기장내 초파리집단에서 약 63%가, 탈지구자기장 집단에서는 약70%, 지구자기장 2배의 집단에서는 65%를, 그리고 지구자기장 8배의 초파리 집단에서는 전체 평균 발생빈도의 74%를 넘는 것으로 관찰되었다. This study was carried out to elucidate influence of magnetic field on sexual selection between sex-linked mutant and the wild type of Drosophila melanogaster collected at keojedo. For the present experiment using population cages, each fly of four cages reared under the influence of four different strengths of magnetic field, that is, the ambient, the without, the double and the eight-fold magnetic field artificially generated by Helmholtz coils. Also the study was analysed emergence frequencies of progeny on counting days due to eggs laying by mated females on a complete food. The over-all mean emergence rates of population of 25 generations reared under the four different magnetic fields, the ambient, the without, the double and the eight-fold, were 20.35%, 24.55%, 25.27% and 29.83%, respectively. The fly population raised under the ambient magnetic field showed a relatively lower emergence rate than any of those raised under the other three magnetic fields. Emergence frequencies of progeny on counting days from 1st to 3rd day under different magnetic fields were above 63%, 70%, 65% and 74%,respectively.

Monte Carlo 방법을 이용한 대기오염 배출률 예측의 불확실성 평가

정효준,김은한,서경석,황원태,한문희 한국환경과학회 2006 한국환경과학회지 Vol.15 No.4