蔓蔘의 methanol 추출액이 mitomycin C의 세포독성 효과에 미치는 영향

田炳薰,鄭遇悅 한국전통의학연구소 1997 한국전통의학지 Vol.7 No.2

만삼은 인삼의 대용으로도 사용할 수 있는 약물로서 가능성이있고,수요가 많은 인삼을 대체할 수 도 있는 약물로 생각된다. 인삼에 대한 임상적 연구와 항암효과에 대한 연구는 많이 있으나 만삼에 대한 연구는 그리 많지 않았다. 그러나 최근에 만삼의 다양한 임상적 효능과 항암효과에 관한 실험등이 중국등을 중심으로 많이 발표 되었다. 蔓蔘의 항암효과는 암세포의 동물이식에 대한 실험,화학적 발암원에 의한 육종에 대한 효과,배양한 종앙세포 등에 대한 실험에서 많이 조사되었다. 蔓蔘에 대한 많은 연구가 있었으나 복강내주사나 in vitro 모델등에서 실시한 것이었고,경구투여를 통한 실험은 거의 없었다. 한편 정상세포에는 손상을 주지않고 오직 암세포만을 살상 하는 항종양성약물은 아직까지 개발되지 않았다. 따라서 항종양성약물의 항종양효과를 상승적으로 증진시키고 부작용을 줄이는 약물의 개발은 절실한 것이다. 때문에 본 논문에서는 Ehrlich carcinoma의 solid tumor와 ascites tumor에 대한 蔓蔘추출물의 항종양효과를 관찰하였다. 蔓蔘추출물이 항암화학요법제인 MMC의 항종양효과를 증가시킬 수 있는가를 살피고,종양세포로의 MMC의 uptake,종양세포의 lysosomal enzymes에 대한 활성 및 MMC의 세포독성에 어떠한 효과를 나타내는 가를 관찰하였다. The influence of the ethanol extract from RADIX CODONOPSITIS(RC) on the lysosome of tumor cells and on the cytocidal effect of mitomycin C(MMC) was investigated. KRG treatment showed an inhibitory effect on the solid form of Ehrlich ascites carcinoma but had no effect on the ascites form. Mitomycin C combined with RADIX CODONOPSITIS(RC) showed stronger antitumor effects at the same time, the activity of lysosomal enzymes in tumor cells was also increased in comparison with that treated with MMC alone. Furthermore, RADIX CODONOPSITIS(RC) promoted the uptake of MMC into the tumor cells and enhanced in vitro the cytotoxicity of MMC against the cultured tumor cells. From these results it was concluded that RADIX CODONOPSITIS(RC) labilized the Iysosomes of tumor cells in vivo, and increased the uptake if MMC into the tumor cells, and that the cytocidal effect of MMC was enhanced by concomitant treatment with RADIX CODONOPSITIS(RC).

人蔘이 산소유리기로 손상된 척수신경세포의 손상에 미치는 영향

田炳勳,文炳淳,姜益賢 대한동의병리학회 1998 동의생리병리학회지 Vol.12 No.1

산소자유기는 중풍인 뇌졸중을 비롯하여 뇌허혈, 파킨슨씨병과 같은 뇌질환의 병리적 요인으로 밝혀짐에 따라 이의 독성효과나 작용현상에 대한 기전을 규명하기 위하여 많은 연구가 진행되어 왔다. 그러나 산소자유기의 독성효과에 대하여 아직까지 이에 대한 자세한 기전이 밝혀져 있지 않고 치료방법 역시 미흡한 실정이다. 본 연구는 산소자유기의 신경독성 효과에 대한 기전을 규명하기 위하여 배양소의 희소돌기아교세포에 여러 농도의 산소자유기를 처리한 다음 산소자유기의 독성효과를 측정하였으며 또한 산소자유기의 독성효과에 대한 인삼추출물의 영향을 MTT assay법으로 조사하여 다음과 같은 결론을 얻었다. xanthine oxidase(XO)와 hypoxanthine(HX)은 처리한 농도에 비례하여 배양 신경세포의 생존율을 현저하게 감소시켰으며, 인삼추출물을 배양 희소돌기아교세포에 전처리한 결과 XO/HX의 독성효과를 유의성있게 방어하였다. 이상의 결과로부터 산소자유기는 배양 소의 희소돌기아교세포에 독성을 나타냈으며 인삼추출물이 산소자유기의 독성을 방어하는데 효과적인 것으로 나타났다. To elucidate the neurotoxic mechanism of oxygen radicals in cultured bovine oligodendrocytes, cytotoxic effect was measured by MTT assay after cultured oligodendrocytes were grown in the medium containing various concentrations of oxygen radicals. In addition, neuroprotective effect of herb extract, Korean red Ginseng was examined by MTT assay in cultured oligodendrocytes. Cell viability of cultured oligodendrocytes was remarkably decreased by xanthine oxidase(XO) and hypoxanthine(HX) in dose-dependent manner, and Korean red Ginseng protected remarkably oxidant-induced neurotoxicity in these cultures. From the above results, it is suggested that oxygen radicals is toxic in cultured bovine oligodendrocytes, and herb extracts such as Korean red Ginseng are effective in blocking the neurotoxicity induced by oxygen radicals in cultured bovine oligodendrocytes.

韓國體育大學生들의 欲求特性에 關한 硏究

全承勳,劉丙烈,朴相龍,金根友 한국체육대학교 부속 체육연구소 1986 체육연구소논문집 Vol.4 No.-

The purpose of this study is to investigate the need traits of KNCPE (Korean national College of Physical Education) students and to find out the number of students to be required special guidance on counceling services. The instrument used investigation was the SNDT (Standardization Need Diagnostic Test) and this include descriptive and validity scales. The results are as follows: 1. Generally, the need of the KNCPE students belong to norm group. 2. The number of students who have abnormal scores in almost every scales are 9 (2.0%; male-5, female-4). 3. The students has above mentioned problem should join in the special guidance on counceling services.

秘方奪命散의 抗炎症效果 및 抗凝固作俑에 대한 硏究

田炳薰,禹元洪,洪知雨 대한동의병리학회 1998 동의생리병리학회지 Vol.12 No.2

丹溪心法에 最初로 收錄되어 一切癰疽 無名惡瘡을 治療하는데 使用되는 秘方奪命散이 염증 및 血栓의 치료에도 유효한 처방으로 생각되어 본 실험을 계획하였으며, 비방탈명산의 抗炎症 作用, 抗血栓作用, 鎭痛效果를 觀察한 結果, 有意性이 認定되었다. 秘方奪命散의 抗炎症 效果를 알아보기 위하여 carragenin을 利用한 局所的浮腫 實驗과 炎症反應時 나타나는 血管透過性亢進으로 因한 蛋白質渗出의 抑制를 觀察하기 위하여 CMC-pouch를 利用하여 蛋白質 渗出을 測定하였다. 醋酸法과 熱板法으로 鎭痛效果를 관찰하였다. 抗血栓效果를 究明하기 위하여 肺性血栓性塞栓症에서의 死亡率, 血小板 凝集度, 血漿凝固活性, 관찰했던 바, 다음과 같은 結果를 얻었다. carragenin으로 誘發한 後肢足蹠의 浮腫은 檢液投與 2,3時間 後에 BTS Ⅱ,Ⅲ群에서 有意性있는 抑制效果과 있었고, CMC-pouch에 의한 蛋白質 渗出은 BTS Ⅱ,Ⅲ群에서 有意性 있는 減少를 보였으나 aspirin藥物 對照群보다는 微弱하였다. 醋酸法과 熱板法에 의해 writhing syndrome, paw licking time, escape time을 측정한 결과, 一定한 鎭痛效果를 確認할 수 있었다. 秘方奪命散의 抗血栓效果는 serotonin과 collagen 混合液으로 誘發한 肺性血栓性 塞栓症에서의 死亡率 減少 血小板 凝集 抑制등의 효과를 보였으나, 혈장응고활성을 측정한 prothrombin time과 activated partial thromboplastin time은 연장되는 결과를 보이기는 하였으나 유의한 결과는 아니었다. This study was designed to elucidate the antiinflammatory, antithrombotic, and analgesic effect of Bibangtalmyungsan and antiflammatory effects was measured by the method of carrageenin induced edema, protein leakage test using CMC-pouch. Death rate, platelet aggregation, plasma coagulation activity, prothrombin time and activated partial thromboplastin time were observed for the measurement of the antithrombotic effect of Bibangtalmyungsan, and the analgesic effect was measured by the acetic acid method and hot plate method. The result was as follows : After 2 or 3 hours of Bibangtalmyungsan administration, carrageenin induced edema and CMC-pouch protein leakage was significantly decreased. The slight anagesic effect of Bibangtalmyungsan extract was confirmed by the observation of writhing syndrome, paw licking time, and escape time. The antithrombotic effect of Bibangtalmyungsan was observed by the decrease of death rate, the inhibition of platelet aggregation, and the increase of antithrombin activity. It increased the prothrombin time and activated partial thromboplastin time.

산국 (Chrysanthemum boreale) 휘발성 추출액 및 EDTA가 미생물 및 인체 치은 섬유아세포에 미치는 영향

차정단,김태영,우원홍,전병훈,김해경,유용욱,김강주,길봉섭 원광대학교 생체재료·매식연구소 2000 원광생체재료·매식 Vol.9 No.1

Chrysanthemum boreale (C. boreale) has been used for the treatment of furuncles, carbuncles, sores, conjunctivitis, headache, vertigo and swollen throats as a folk medicine in Korea, but there is little report about experiment evidence of antimicrobial activity. To investigate the antifungal and antibacterial activity of Chrysanthemum boreale(C. boreale) volatile substance and EDTA, the growth inhibition against Aspergillus nidulans (A. nidulans), Fusarium oxysporum (F. oxysporum), Alternaria mali(Al. mali), Candida albicans (C. albicans), staphylococcus aureus (S. aureus), Streptococcus pyogenes(S. pyogenes), streptococcus mutans (S. mutans), Escherichia coli(E. coli), Helicobacter pylori (H. pylori) and Porphyromonas gingivalis (P. gingivalis) was monitored by agar dilution and broth microdilution method. The antifungal effect of C. boreale on A. nidulans, F. oxysporum, Al. mali and C. albicans was shown at the concentration of 0.04%. The antibaterial effect of C. boreale on S. aureus, S. pyogenes, S. mutans and H. pylori was shown at the concentration of 0.04%. However, the growth was inhibited at the concentration of 0.04% irrespective of the presence of EDTA 0.125% was much higher than higher than that of several concentration of volatile substance. The minimum inhibitory concentrations of volatile substance were 0.16% against S. aureus, 0.04% against S. pyogenes, 0.02% against S. mutans, 0.40% against E. coli, and 0.18% against C. albicans. The growth of human gingival fibroblasts was not affected by C. boreale volatile substances at the concentration of 0.04%. These results indicate that volatile substance from C. boreale may have biologically toxic activities to the microorganisms.

EFFECT OF THE METHANOL EXTRACT OF KOREAN TRADITIONAL MEDICINE HERB OLDENLANDIAE DIFFUSAE HERBA ON PROLIFERATION AND DIFFERTATION OF CANCER CELL

Jeon,Byung-Hun,Moon,Gu,Woo,Won-Hong 대한동의병리학회 1998 동의생리병리학회지 Vol.12 No.2

백화사설초는 여러 가지 암세포의 성장을 억제할 수 있는 항암효과가 뛰어난 약재로, 근래에 많이 암환자에 활용되어지고 있으며, 실제 실험적으로도 그 효과가 입증되어지고 있다. 본 연구에서는 백화사설초가 사람 암세포의 증식과 분화에 어떠한 효과를 미치는지를 알아보기 위하여 monocytic leukemic cell line인 U937을 백화사설초 추출물만을 처리하고, 또한 백화사설초(10 ㎍/ml)의 농도에서 활성화된 단핵세포(ODH-MNC-CM)를 사용하였다. 그 결과 백화사설초 단독으로 사용할 경우 암세포의 분화를 유도하는 작용은 가지고 있지 않았지만 백화사설초가 Leukemic U937 세포에 대한 증식억제와 분화를 유도하는 IFN-γ, TNF-α와 같은 cytokine의 생산을 야기할 수 있다는 것을 알았으며, 백화사설초 추출물의 항종양효과와 암세포분화효과는 이러한 cytokines의 생성과 관련된 것으로 생각된다. Oldenlandiae diffusae Herba is herb medicine with antitumor activity capable of suppressing the growth of some cancer cell lines. In the present study, we have isolated a methanol extraction of Oldenlandiae diffusae Herba(ODH) and investigated its effect on the proliferation and differentiation of human cancer cell line using an in vitro culture system. Our results showed that the conditioned medium from ODH(10 ㎍/ml)-stimulated blood mononuclear cells (ODH-MNC-CM) treatment induced about 50% of the cells differentiating into mature monocytes/macrophages of CD 11b, CD 14, and CD68. Yet, the differentiated U937 cells also had functions of phagocytosis and superoxide production. However, ODH alone or normal MNC-CM had no such effects. The levels of interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and interleukin (IL)-1 were very low in normal MNC-CM, and they were greatly increased in MNC-CM prepared with ODH stimulation. Antibody neutralization studies further revealed that the tumoricidal and differentiating effects of ODH-MNC-CM were mainly derived from the elevated cytokines, especially IFN-γ and TNF-α. These two cytokines acted synergistically on inhibiting cell growth and inducing differentiation of the target U937 cells.

B16 흑색종 세포의 폐전이에 대한 소암산1의 억제효과

전병훈(Byung Hun Jeon),김원신(Won Sin Kim) 한의병리학회 2002 동의생리병리학회지 Vol.16 No.6

蘇木 물추출물의 G2/M기 정지를 통한 U937세포의 성장억제 효과

전병제(Byung Jae Jeon),주성민(Sung Min Ju),양현모(Hyun Mo Yang),김보현(Bo Hyun Kim),김원신(Won Sin Kim),전병훈(Byung Hun Jeon) 한의병리학회 2010 동의생리병리학회지 Vol.24 No.1

鷄鳴散이 endotoxin으로 誘發된 白鼠의 血栓症에 미치는 影響

田炳薰,鄭鉉雨,李炳周 대한동의병리학회 1992 동의생리병리학회지 Vol.7 No.-

This study was performed to investigate the effect of Keomyungsan(鷄鳴散) on the intravascular coagulation induced by endotoxin in rats. The intravasular coagulation was induced by injecting endotoxin in the caudal vein of rats. And these rats were treated with extract of Keomyungsan(鷄鳴散) 1.5ml/200g (Group Ⅰ) and Keomyungsan(鷄鳴散) 3.0ml/200g (Group Ⅱ), which were administered orally. Then, the number of blood cells, platelets, concentration of prothrombin time, fibrinogen and FDP (fibrin-fibrinogen degradation products) were measured. The following results were obtained : 1. The number of platelets increased in the Group Ⅱ compared with the control group, but it is not signifiant. 2. The prothrombin time was shortened significantly in the Group Ⅱ compare with the control group. 3. The concentration of fibrinogen increased significantly in the trial group compared with the control group. 4. The degree of concentration of FDP decreased significantly in the Group Ⅱ compared with the control group. According to the above results, it is considered that Keomyungsan(鷄鳴散) has the significant effects on thrombosis. Therefore, Keomyungsan(鷄鳴散) seem to be applicable to the diseases related to thrombosis.

Short Stroke Control Model for Improving Width Precision at Head and Tail of Slab in Hot Vertical–Horizontal Rolling Process

Byung-Min Kim,Kyung-Hun Lee,Jun-Bae Jeon 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.21 No.4

The objectives of this study were to analyze the width changes about the head and tail ends of the slab occurring duringvertical–horizontal rolling using fi nite element (FE) analysis, and to derive an appropriate short stroke control (SSC) modelfor vertical–horizontal rolling based on the results of analyses. The relationship between the process variables and slabdeformation about the head and tail ends, including width shrinkage and length of width shrinkage, was derived using thelinear regression method. From considering the amount of slab deformation, an advanced SSC model for vertical–horizontalrolling process has been developed. The proposed SSC model was verifi ed via an FE simulation. Vertical rolling simulationswere performed using the roll gap that was calculated via the respective SSC model for each steel slab. Horizontal rollingsimulations were also conducted. Cropping lengths about the head and tail ends that were regulated by width control standardsof the specifi c work site were compared.