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Synergistic anti-inflammatory effects of Laminaria japonica fucoidan and Cistanche tubulosa extract
Jangbeen Kyung,Dajeong Kim,Dongsun Park,Yun-Hui Yang,Ehn-Kyoung Choi,Sung-Pyo Lee,Tae-Su Kim,Yoon-Bok Lee,Yun-Bae Kim 한국실험동물학회 2012 Laboratory Animal Research Vol.28 No.2
The anti-inflammatory effects of fuciodan and Cistanche tubulosa (CT) extract were investigated in vitro macrophage culture system and in vivo carrageenan-induced air pouch inflammation model. CT extract inhibited nitric oxide production from activated RAW 264.7 macrophage cells, while fucoidan was inactive. In vivo air pouch inflammation model, carrageenan-induced vascular exudation and increased nitric oxide and prostaglandin E2 concentrations in the exudates were synergistically suppressed by co-administration of fucoidan or CT extract. Moreover, tissue inflammation was substantially attenuated by the combinational therapy. However, there was no synergistic effect against the inflammatory cell infiltration, although fucoidan and CT extract each markedly reduced the cell numbers. Therefore, it is suggested that fucoidan blocks infiltration of inflammatory cells, while CT extract inhibits activation of the cells, and that their combinational treatment could be a promising candidate for the relief of various types of inflammation.
Dongsun Park,Jangbeen Kyung,Dajeong Kim,Seock-Yeon Hwang,Ehn-Kyoung Choi,Yun-Bae Kim 한국실험동물학회 2012 Laboratory Animal Research Vol.28 No.1
The effects of polarized-light therapy (PLT) on high-cholesterol diet (HCD)-induced hypercholesterolemia and atherosclerosis were investigated in comparison with that of lovastatin in rabbits. Hypercholesterolemia was induced by feeding male New Zealand white rabbits with 1% cholesterol in diet for 2 weeks and maintained with 0.5% cholesterol for 6 weeks, followed by normal diet for 2 weeks for recovery. Lovastatin (0.002% in diet) or daily 5-min or 20-min PLT on the outside surface of ears was started 2 weeks after induction of hypercholesterolemia. Hypercholesterolemic rabbits exhibited great increases in serum cholesterol and low-density lipoproteins (LDL) levels, and finally severe atheromatous plaques formation covering 57.5% of the arterial walls. Lovastatin markedly reduced both the cholesterol and LDL, but the reducing effect (47.5%) on atheroma formation was relatively low. By comparison, 5-min PLT preferentially decreased LDL, rather than cholesterol, and thereby potentially reduced the atheroma area to 42.2%. Notably, 20-min PLT was superior to lovastatin in reducing both the cholesterol and LDL levels as well as the atheromatous plaque formation (26.4%). In contrast to the increases in blood alanine transaminase and aspartate transaminase following lovastatin treatment, PLT did not cause hepatotoxicity. In addition, PLT decreased platelets and hematocrit level. The results indicate that PLT attenuates atherosclerosis not only by lowering blood cholesterol and LDL levels, but also by improving blood flow without adverse effects. Therefore, it is suggested that PLT could be a safe alternative therapy for the improvement of hypercholesterolemia and atherosclerosis.
Kim, Dajeong,Kyung, Jangbeen,Park, Dongsun,Choi, Ehn-Kyoung,Kim, Kwang Sei,Shin, Kyungha,Lee, Hangyoung,Shin, Il Seob,Kang, Sung Keun,Ra, Jeong Chan,Kim, Yun-Bae unknown 2015 Stem cells translational medicine Vol.4 No.10
<P>: Aging brings about the progressive decline in cognitive function and physical activity, along with losses of stem cell population and function. Although transplantation of muscle-derived stem/progenitor cells extended the health span and life span of progeria mice, such effects in normal animals were not confirmed. Human amniotic membrane-derived mesenchymal stem cells (AMMSCs) or adipose tissue-derived mesenchymal stem cells (ADMSCs) (1 ?? 10(6) cells per rat) were intravenously transplanted to 10-month-old male F344 rats once a month throughout their lives. Transplantation of AMMSCs and ADMSCs improved cognitive and physical functions of naturally aging rats, extending life span by 23.4% and 31.3%, respectively. The stem cell therapy increased the concentration of acetylcholine and recovered neurotrophic factors in the brain and muscles, leading to restoration of microtubule-associated protein 2, cholinergic and dopaminergic nervous systems, microvessels, muscle mass, and antioxidative capacity. The results indicate that repeated transplantation of AMMSCs and ADMSCs elongate both health span and life span, which could be a starting point for antiaging or rejuvenation effects of allogeneic or autologous stem cells with minimum immune rejection.</P>
Anti-obesity effects of Rapha diet<SUP>®</SUP> preparation in mice fed a high-fat diet
Jihyun Kim,Jangbeen Kyung,Dajeong Kim,Ehn-Kyoung Choi,Paul Bang,Dongsun Park,Yun-Bae Kim 한국실험동물학회 2012 Laboratory Animal Research Vol.28 No.4
The anti-obesity activities of Rapha diet<SUP>®</SUP> preparation containing silkworm pupa peptide, Garcinia cambogia, white bean extract, mango extract, raspberry extract, cocoa extract, and green tea extract were investigated in mice with dietary obesity. Male C57BL/6 mice were fed a high-fat diet (HFD) containing 3% Rapha diet<SUP>®</SUP> preparation for 8 weeks, and blood and tissue parameters of obesity were analyzed. The HFD markedly enhanced body weight gain by increasing the weights of epididymal, perirenal, and mesenteric adipose tissues. The increased body weight gain induced by HFD was significantly reduced by feeding Rapha diet<SUP>®</SUP> preparation, in which decreases in the weight of abdominal adipose tissue and the size of abdominal adipocytes were confirmed by microscopic examination. Long-term feeding of HFD increased blood triglycerides and cholesterol levels, leading to hepatic lipid accumulation. However, Rapha diet<SUP>®</SUP> preparation not only reversed the blood lipid levels, but also attenuated hepatic steatosis. The results indicate that Rapha diet<SUP>®</SUP> preparation could improve HFD-induced obesity by reducing both lipid accumulation and the size of adipocytes.
Dajeong Kim,Dongsun Park,Jangbeen Kyung,Yun-Hui Yang,Ehn-Kyoung Choi,Yoon-Bok Lee,Hyun-Kyu Kim,Bang Yeon Hwang,Yun-Bae Kim 한국실험동물학회 2012 Laboratory Animal Research Vol.28 No.2
Anti-inflammatory effects of Houttuynia cordata supercritical extract (HSE) were investigated in rat carrageenan-air pouch model. Oral administration of HSE (50-200 ㎎/㎏) suppressed carrageenaninduced exudation and albumin leakage, as well as inflammatory cell infiltration at a high dose (200 ㎎/㎏). Intraperitoneal injection of dexamethasone (2 ㎎/㎏) only decreased exudation and cell infiltration, while indomethacin (2 ㎎/㎏, i.p.) reduced exudate volume and albumin content without influence on the cell number. HSE lowered tumor-necrosis factor-α (TNF-α) and nitric oxide (NO), as well as prostaglandin E2 (PGE2). Dexamethasone only reduced TNF-α and NO, while indomethacin decreased PGE2. The results indicate that HSE exhibits anti-inflammatory effects by inhibiting both TNF-α-NO and cyclooxygenase-2-PGE2 pathways.
Ja-Young Jang,Jingmei Cai,Jihyun Kim,Jangbeen Kyung,Dajeong Kim,Ehn-Kyoung Choi,Youngeun Kim,Kwang-Sei Kim,Dongsun Park,Hyun-Gu Kang,Yun-Bae Kim 한국실험동물학회 2013 Laboratory Animal Research Vol.29 No.1
According to a high anti-osteoporotic efficacy of Sigma Anti-bonding Molecule Calcium Carbonate (SAC), repeated-dose toxicities of SAC were investigated to assess its feasibility as drug or functional food ingredient. Male ICR mice were given drinking water containing 0.006, 0.02 or 0.06% SAC for 4 weeks. SAC feeding decreased the body weights and feed and water consumptions of mice in a dose-dependent manner, especially, leading to severe emaciation and 70% death in 3 weeks in the high-dose (0.06%) group. Not only kidney and heart weights, but also the levels of blood urea nitrogen, creatinine, aspartate transaminase, and creatine phospokinase significantly increased after SAC administration, indicative of nephrotoxicity and cardiotoxicity. Such renal and cardiac toxicities were also confirmed by microscopic findings, exhibiting renal crystals and cardiac fibrosis, which may be due to the insoluble crystal formation and calcium overload, respectively. In conclusion, it is suggested that no observed adverse effect level of SAC is lower than 0.006% in mice, and that a long-term intake may cause serious adverse effects on renal and cardiac functions.