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Cloning and Molecular Characterization of β-1,3-Glucan Synthase from Sparassis crispa
( Yun Hui Yang ),( Hyeon Woo Kang ),( Hyeon Su Ro ) 한국균학회 2014 韓國菌學會誌 Vol.42 No.2
A β-glucan synthase gene was isolated from the genomic DNA of polypore mushroom Sparassis crispa, which reportedly produces unusually high amount of soluble β-1,3-glucan (β-glucan). Sequencing and subsequent open reading frame analysis of the isolated gene revealed that the gene (5,502 bp) consisted of 10 exons separated by nine introns. The predicted mRNA encoded a β-glucan synthase protein, consisting of 1,576 amino acid residues. Comparison of the predicted protein sequence with multiple fungal β-glucan synthases estimated that the isolated gene contained a complete N-terminus but was lacking approximately 70 amino acid residues in the C-terminus. Fungal β-glucan synthases are integral membrane proteins, containing the two catalytic and two transmembrane domains. The lacking C-terminal part of S. crispa β-glucan synthase was estimated to include catalytically insignificant transmembrane α-helices and loops. Sequence analysis of 101 fungal β-glucan synthases, obtained from public databases, revealed that the β-glucan synthases with various fungal origins were categorized into corresponding fungal groups in the classification system. Interestingly, mushrooms belonging to the class Agaricomycetes were found to contain two distinct types (Type I and II) of β-glucan synthases with the type-specific sequence signatures in the loop regions. S. crispa β-glucan synthase in this study belonged to Type II family, meaning Type I β-glucan synthase is expected to be discovered in S. crispa. The high productivity of soluble β-glucan was not explained but detailed biochemical studies on the catalytic loop domain in the S. crispa β-glucan synthase will provide better explanations.
Effects of β-Lapachone on Gastric Secretion
Yun-Bae Kim, Dongsun Park, In Geun Cho, Yun-Hui Yang, Jangbeen Kyung, Dajeong Kim, Ehn-Kyoung Choi, Tae Hwan Kwak3, Sang Ku Yoo 충북대학교 동물의학연구소 2011 Journal of Biomedical and Translational Research Vol.12 No.3
The effects of β-lapachone on gastric secretion were investigated. The pylorus of male Sprague-Dawley rats was ligated and intraduodenally injected with β-lapachone, and the volume, pH, free HCl, and total acidity of gastric fluid were measured 6 hours after the operation. Treatment with β-lapachone resulted in dose-dependent inhibition of gastric secretion Gastric fluid was reduced to 42.9% of control level by 100 mg/kg of β-lapachone, leading to an increase of pH to 6.70 from 1.85 in the control group. In parallel with the increase of pH, at this dosage, free HCl and total acidity decreased to 16.7% and 12.0%, respectively, of control levels. β-Lapachone exhibited ED50 values of 72, 46, and 47 mg/kg for inhibition of gastric volume, free HCl, and total acidity, respectively, implying a superior efficacy on gastric acid to volume. In comparison, pantoprazole (30 mg/kg) reduced the volume, free HCl and total acidity of gastric fluid to 53.0%, 26.0%, and 25.0%, respectively, of control levels, resulting in an increase in pH to 6.36. In the current study, it was confirmed that β-lapachone at an appropriate dose (100 mg/kg) exerted a higher inhibitory effect on gastric secretion than pantoprazole (30 mg/kg), a well-known proton-pump inhibitor. Therefore, it is suggested that β-lapachone could be a candidate compound for prevention or treatment of gastric ulcers induced by diverse psychological and physical stimuli.
Anti-Helicobacter pylori effects of IgY from egg york of immunized hens
Yun-Hui Yang,Dongsun Park,Goeun Yang,Sun Hee Lee,Dae Kwon Bae,Jangbeen Kyung,Dajeong Kim,Ehn-Kyoung Choi,Jae-Cheol Son,Seock-Yeon Hwang,Yun-Bae Kim 한국실험동물학회 2012 Laboratory Animal Research Vol.28 No.1
Effects of egg york containing IgY specific for Helicobacter pylori on the bacterial growth and intragastric infection were investigated in comparison with a proton-pump inhibitor pantoprazole. For in vitro antibacterial activity test, H. pylori (1*108 CFU/mL) was incubated with a serially diluted IgY for 3 days. As a result, IgY fully inhibited the bacterial growth at 16 mg/mL, which was determined to a minimal inhibitory concentration. In vivo elimination study, male C57BL/6 mice were infected with the bacteria by intragastric inoculation (1*108 CFU/mouse) 3 times at 2-day intervals, and 2 weeks later, orally treated twice a day with 50, 100, 200 or 500 mg/kg IgY for 18 days. After the final administration, biopsy sample of the gastric mucosa was assayed for the bacterial identification via urease, oxidase, catalase, nitrate reduction and H2S tests in addition to microscopic examination for mucosal inflammation. In CLO kit test, 75, 50, 12.5 and 12.5% of the animals revealed positive reaction following treatment with 50, 100, 200 and 500 mg/kg IgY, respectively, resulting in a superior efficacy at 200 mg/kg than 30 mg/kg pantoprazole that displayed 75% elimination. The CLO test results were confirmed by bacterial identification. Microscopic examination revealed that H. pylori infection caused severe gastric mucosal inflammation, which were not observed in the CLO-negative mice following treatment with IgY or pantoprazole. Taken together, IgY inhibited the growth of H. pylori, and improved gastritis and villi injuries by eliminating the bacteria from the stomach. The results indicate that IgY could be a good candidate overcoming tolerance of antibiotics for the treatment of H. pylori-mediated gastric ulcers.
Augmentation by Hypercholesterolemia of Amyloid β Peptide-Induced Learning and Memory Deficit
Yun-Bae Kim,Dongsun Park,Jiyoung Oh,Tae Kyun Kim,Young Jin Cho,Sun Hee Lee,Dae-Kwon Bae,Yun-Hui Yang,Goeun Yang,Seock-Yeon Hwang 충북대학교 동물의학연구소 2009 Journal of Biomedical and Translational Research Vol.10 No.2
The present study was carried out to establish an animal model, displaying long-term learning and memory dysfunction, since single intracerebroventricular (icv) injection of amyloid β peptide (Aβ) causes a short-term memory impairment. Male ICR mice were fed a high-cholesterol diet (HCD) containing 3% cholesterol, 1% corn oil and 0.5% cholic acid, and 1 week later, icv injected with Aβ<sub>1-42</sub> (5 μg/head). Learning/ memory function was assessed via passive avoidance performances 1 day and 2, 4, and 6 weeks after Aβ<sub>1-42</sub> injection, in addition to blood biochemical analyses for lipid profiles and hepatic function. Total cholesterol, lowdensity lipoproteins and hepatic dysfunction parameters markedly increased, while high-density lipoproteins were reduced following HCD feeding. Whereas single injection of Aβ induced temporary memory loss 1 day after administration, exhibiting full recovery after 2 weeks, Aβ treatment in combination with HCD feeding lasted the learning/memory impairment up to 6 weeks. Therefore, it is suggested that hypercholesterolemia augments Aβ-induced memory loss, and that Aβ injection plus HCD feeding could be a long-term memorydeficit model suitable for long-term treatment with drugs or stem cells.
Cloning and Molecular Characterization of ${\beta}$-1,3-Glucan Synthase from Sparassis crispa
Yang, Yun Hui,Kang, Hyeon-Woo,Ro, Hyeon-Su The Korean Society of Mycology 2014 Mycobiology Vol.42 No.2
A ${\beta}$-glucan synthase gene was isolated from the genomic DNA of polypore mushroom Sparassis crispa, which reportedly produces unusually high amount of soluble ${\beta}$-1,3-glucan (${\beta}$-glucan). Sequencing and subsequent open reading frame analysis of the isolated gene revealed that the gene (5,502 bp) consisted of 10 exons separated by nine introns. The predicted mRNA encoded a ${\beta}$-glucan synthase protein, consisting of 1,576 amino acid residues. Comparison of the predicted protein sequence with multiple fungal ${\beta}$-glucan synthases estimated that the isolated gene contained a complete N-terminus but was lacking approximately 70 amino acid residues in the C-terminus. Fungal ${\beta}$-glucan synthases are integral membrane proteins, containing the two catalytic and two transmembrane domains. The lacking C-terminal part of S. crispa ${\beta}$-glucan synthase was estimated to include catalytically insignificant transmembrane ${\alpha}$-helices and loops. Sequence analysis of 101 fungal ${\beta}$-glucan synthases, obtained from public databases, revealed that the ${\beta}$-glucan synthases with various fungal origins were categorized into corresponding fungal groups in the classification system. Interestingly, mushrooms belonging to the class Agaricomycetes were found to contain two distinct types (Type I and II) of ${\beta}$-glucan synthases with the type-specific sequence signatures in the loop regions. S. crispa ${\beta}$-glucan synthase in this study belonged to Type II family, meaning Type I ${\beta}$-glucan synthase is expected to be discovered in S. crispa. The high productivity of soluble ${\beta}$-glucan was not explained but detailed biochemical studies on the catalytic loop domain in the S. crispa ${\beta}$-glucan synthase will provide better explanations.
목재제품 규격,품질 표시제도 국내 도입과 정착을 위한 제언
김윤희 ( Yun Hui Kim ),여환명 ( Hwan Myeong Yeo ),방성준 ( Sung Jun Pang ),양상윤 ( Sang Yun Yang ),강승모 ( Sung Mo Kang ),황권환 ( Kweon Hwan Hwang ) 한국목재공학회 2013 목재공학 Vol.41 No.3
목재산업은 녹색성장과 더불어 한국의 차세대 성장동력으로써 주목받고 있다. 또한, 지속적인 목재의 이용과 목재산업의 활성화를 위하여 「목재의 지속가능한 이용에 관한 법률」이 2012년 제정되었다. 자연으로부터 얻어진 목재자원의 가치를 향상시키고 선순환 구조를 지닌 목재제품이용의 확대를 통해 지속적인 성장을 동반하기 위해서는 목재제품 품질관리가 필요하다. 이 논문에서는 국내외 목재제품의 규격·품질표시제도를 살펴보고 국내 목재산업에의 적용을 위한 방안을 모색해 보고자 하였다. 제재목을 중심으로 미국, 캐나다, 뉴질랜드와 일본의 규격·품질표시제도의 운영현황에 대하여 조사하였으며 목재제품에 대한 규격·품질표시제도는 시스템 진화를 통해 생산자의 자발적 참여로 운영되는 제3자 인증제도로 발전됨을 확인할 수 있었다. 국내 목재제품의 규격·품질표시제도의 활성화를 위해서는 도입기에는 산림청 중심으로 일원화 시스템을 구축하여 조기정착을 유도하고 성장기 및 성숙기를 통해 인식구조 개선과 생산자의 자발적 참여를 위한 인센티브 개발 등의 기반을 마련하여 제3자 인증제도로 발전시켜야 할 것이다. Wood-based products market is new growth engine industry of “Green growth policy”, Korean government establiushed a law, sustainable use of wood, for expanding wood and wood-based products market in 2012. This study was conducted to seek sustainable measures for conservation and management of valuable natural resources, offer expanding quality assurance services for wood-based products, and explore measures for expanding wood-based products labeling system. Through discussed domestic and overseas trends and literatures that concern wood-based products labeling system and investigated case studies that were presently operational. For expanding quality assurance services of wood-based products, Korea Forest Service operates a wood-based products labeling system in integrated management through variety awareness and specialist assessment for each product This process will make enhanced wood-based products labeling system expanded gradually. To evolve into 3rd party quality assurance services on maturity age of labeling system, public communication will help improve awareness of producer and consumer. approprtate incentive program will attract voluntary participation from woodbased product industry. Producers consider environmental ethics in their business and consumer should exercise their right to know,