미각장애와 TAS1R3 및 GNAT3 유전자의 다형성과의 연관성

Jae-Woong Bae(배재웅),Un-Kyung Kim(김언경),Tae-Jun Kwon(권태준),Su-Jin Choi(최수진),Mi-Kyung Ye(예미경) 한국생명과학회 2011 생명과학회지 Vol.21 No.3

단맛은 우리 몸에 열량을 공급하는 역할을 담당하는 중요함 감각으로 인지도가 개인마다 조금씩 다르다고 알려져 있으나, 이에 대한 분자수준의 연구는 아직 부족한 실정이다. 본 연구에서는 미각 장애에 미치는 유전적 요인에 대해 알아보고자 50명의 미각 환자 및 100명의 정상인을 대상으로 단맛 민감도 차이와 연관이 있는 TAS1R3 및 GNAT3유전자의 다형성 간의 관련성을 알아보았다. TAS1R3 유전자 rs307355 및 rs35744813의 유전자형과 대립유전자의 빈도는 미각 장애 환자군과 대조군 간에 통계적으로 유의한 차이가 있었으며, 두 다형성에 대한 일배체형을 분석한 결과, C-C 및 T-T의 두 종류만이 검출되었으며 환자군과 대조군 간의 일배체형 빈도 간에도 통계적으로 유의한 차이를 보였다. GNAT3유전자에서는 rs7792845의 유전자형 빈도가 환자군과 대조군간에 유의적인 차이를 나타냈었으나, 대립유전자빈도에서는 차이가 없었다. 이러한 연구결과는 단맛의 민감도 차이에 영향을 미치는 것으로 보고된 TAS1R3 및 GNAT3 유전자의 다형성에 대한 한국인 집단에서의 유전자형 빈도를 조사함으로써 집단유전학적 연구를 위한 기초자료를 제공하고 미각장애환자군과의 비교분석을 통해 TAS1R3 및 GNAT3유전자의 다형성이 연관성이 있을 가능성이 있음을 제시해 줌으로써 향후 미각장애를 진단하기 위한 검사시 지표로 활용될 수 있으리라 생각된다. 위에 제시한 연구결과는 향후 추가적인 샘플링을 통해 보다 많은 환자군과 대조군에 대한 추가적인 연구가 수행되어야 할 것이다. Taste sensation plays a crucial role in selecting and ingesting foods with different qualities which convey information about their nutrient content and/or safety. Sweetness is one of the five modalities in humans and serves as an energy resource for metabolism. There are reports on allelic polymorphisms which influence perception of sweetness in mice and humans. Since the influence of genetic factors on taste disorder has not been studied, we investigated the association of genetic polymorphisms in TAS1R3 and guanine nucleotide binding protein, alpha transducing 3 (GNAT3) genes and taste disorder. A total of 150 individuals composed of 50 patients with taste disorder and 100 healthy controls were recruited for the study and PCR-mediated directing sequencing method was used to genotype for two different single nucleotide polymorphisms (SNPs) - rs307355 (T>C) and rs35744813 (T>C) in the TAS1R3gene, and rs7792845 (T>C) and rs1524600 (C>T) in the the GNAT3gene. The allele and genotype frequencies of rs307355 and rs35744813 in the TAS1R3gene showed a significant association between patients with taste disorder (p=0.022 and p=0.013 in both of SNPs, respectively). In addition, the frequency of T-T haplotype in the TAS1R3gene was higher in taste disorder cases than in the controls (OR, 1.93: 95%. CI, 1.09?3.39, p=0.022). In the GNAT3, the genotype frequency of rs7792845 in the patients was also different from the controls (p=0.048), but allele frequency was not significantly associated in either group. Our result provides the frequencies of SNPs and haplotypes of the TAS1R3 and GNAT3genes for the fundamental information of nutrigenetics in perception of the taste of sweetness in the Korean population. Also, the study suggests that the allelic polymorphisms of TAS1R3 and GNAT3genes may be useful as a molecular marker for evaluating patients with taste disorder. Further studies with large samples are required to clarify our observation.

Development of Small Object Detection for Maritime Accidents Using Deep Learning

Wonho Jung(정원호),Jae Woong Bae(배재웅),Yong-Hwa Park(박용화) 대한기계학회 2020 대한기계학회 춘추학술대회 Vol.2020 No.12

한국인의 단맛수용체유전자 TAS1R2 다형성분석 및 일배체형 연구

Hye-Jin Lee(이혜진),Jae-Woong Bae(배재웅),Tae-Jun Kwon(권태준),Borum Sagong(사공보름),Un-Kyung Kim(김언경) 한국생명과학회 2010 생명과학회지 Vol.20 No.3

단맛은 인간이 느낄 수 있는 다섯 가지 감각 중 하나로, 열량을 제공하며 식욕을 결정하는데 중요한 요인이다. 인간이 맛물질을 느끼는 민감도 차이에 유전적인 요인이 중요한 역할을 한다는 사실이 알려진 바, 본 연구에서는 한국인 98명을 대상으로 단맛을 결정하는 미각수용체 TAS1R2 유전자에 대해 염기서열분석법을 이용한 단일염기 다형성 종류 및 빈도, 그리고 일배체형 분석을 수행하였다. 그 결과, TAS1R2 유전자로부터 총 12종류의 SNP이 검출되었으며 약 70%는 아미노산 치환을 일으키는 변이로 확인되었다. 특히, 231번째와 950번째 변이는 본 연구를 통해 처음으로 발견된 새로운 것으로 한국인 집단에서 특이적으로 존재하는 SNP일 가능성이 높다고 판단된다. 일배체형 분석결과에 따르면, 발견된 20 종류 일배체형 중 세 가지가 주로 한국인이 가지는 것으로 확인되었다. 본 연구결과 발견된 TAS1R2 유전자의 SNP은 향후 단맛물질을 감지하는 인간의 민감도차이를 결정하는데 유전적 요인으로 작용하는지 알아보는데 중요한 기초자료를 제시해 주리라 생각되며 맞춤형 식단 등 영양유전학 분야에 응용될 수 있을 것이다. Sweetness plays an important role in providing calories and promoting appetite for food. Since it has been known that genetic factor (s) is involved in individual differences in taste sensitivity in humans, this study aimed to examine genetic variations of the TAS1R2 gene, one of the components for tasting sweet compounds, by using DNA sequencing analysis from 98 unrelated Korean subjects. As a result, 12 different single nucleotide polymorphisms (SNPs) were identified in the hTAS1R2 gene and most of them were nonsynonymous. Also, two novel SNPs were found for the first time in this study. It was noted that the frequencies of these SNPs were common in the Korean population. 20 different haplotypes with coding SNPs (cSNPs) were also found in this study. Three out of these haplotypes were common, showing frequencies of > 10%. The repertoire and frequencies of cSNPs and haplotypes in the hTAS1R2 gene will provide information that will help identify a functional ligand receptor common in the Korean population.

Studies on Secretion of Catecholamines evoked by Panaxadiol in the Isolated Rabbit Adrenal Gland

임동윤,박규백,김규형,최철희,배재웅,김무원,Lim, Dong-Yoon,Park, Kyu-Baik,Kim, Kyu-Hyeong,Choi, Cheol-Hee,Bae, Jae-Woong,Kim, Moo-Won The Korean Society of Pharmacology 1988 대한약리학잡지 Vol.24 No.1

본 연구에서 한국산 인삼의 활성성분의 하나인 Panaxadiol(PD)에 대한 가토적출부신에서 카테콜아민(CA)의 분비작용과 작용기전을 파악하고자 실험을 시행하여 다음과 같은 결과를 얻었다. $PD(400{\mu}g)$을 가토적출부신에 투여하였을 때 카테콜아민의 분비를 의의있게 증가시켰다. PD의 이러한 CA 분비작용은 atropine 처치로 현저히 억제되었다. Physostigmine 전처치시 PD 뿐만 아니라 Ach의 CA 분비작용은 뚜렷이 증가되었다. 그러나 chlorisondamine 전처치로 PD나 Ach의 분비 효과는 억제되었다. 또한 $PD(400{\mu}g/30\;min)$을 주입한 후에 Ach의 CA 분비 효과는 오히려 강화되었다. PD나 Ach의 작용은 adenosine 전처러시 현저히 증강되었다. EGTA(5mM)와 함께 Ca-free Krebs액으로 30분 주입한 경우에 Ach의 분비작용은 거의 전적으로 차단되었으며, PD의 작용도 약화되었다. 이상의 실험결과로 보아, PD는 가토적출부신에서 $Ca^{++}$ 의존적으로 CA분비를 증가시키며, 이러한 작용은 cholinergi muscarinic 및 nicotinic receptor의 흥분작용에 기인하며, chromaffincell 대한 일부 직접작용도 개재되어 나타나는 것으로 사료된다. The effect of Panaxadiol(PD), which is an active component of Korean Ginseng Saponins, on the secretion of catecholamines (CA) from the rabbit adrenal gland and its mode of action were investigated in the present study. $PD(400{\mu}g)$ increased significantly the secretion of CA from the isolated perfused rabbit adrenal gland. PD-induced secretion of CA was reduced markedly by treatment of atropine, CA secretion induced by Ach or PD was potentiated significantly by physostigmine-treatment. Chlorisondamine did inhibit CA secretion of PD or Ach. Perfusion of $PD(400{\mu}g)$ for 30 min enhanced the secretory activity of CA by Ach. Ouabain weakened the secretory response induced by PD but rather enhanced the response by Ach. Adenosine-treatment resulted in marked enhancement of CA secretion by PD or Ach, Pefusion with $Ca^{2+}-free$ Krebs containing EGTA (5 mM) for about 30 min totally blocked secretory effect induced by Ach and also weakened that by PD. From the above experimental results, it is suggested that PD causes secretion of catecholamines from the rabbit adrenal gland by a calcium-dependent exocytotic mechanism. The secretory effect of PD is due to the stimulation of cholinergic muscarinic and nicotinic receptors present in the adrenal gland and partly to a direct action on the chromaffin cell itself.

Development of Small Target Detection for Maritime Accident Monitoring Using 3D Game-Based Deep Learning

Wonho Jung(정원호),Sungjin Cheong(정성진),Jae Woong Bae(배재웅),Yong-Hwa Park(박용화) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.11

It is important to recognize the drowning person as soon as possible in maritime accidents. In real maritime accidents, it is difficult to identify the drowning person because of their small size compared to the marine environment. To solve this problem, this paper presents a methodology to detect small target using commercial games with 3D graphical engines. Proposed methodology combines as following four steps: (1) divide high-resolution original image into several small patches, (2) image processing using CLAHE and Canny edge detection, (3) detecting small targets using convolutional neural networks (4) restore patches into original image. To detect small target in the high-resolution original image, small patches and image processing techniques are considered to raise the signal-to-noise ratio of the small target. The small patches are uses as test data of convolutional neural networks (CNN), the softmax values of each patch are displayed on the reconstructed image. To enhance the accuracy of CNN, virtual image data acquired from the commercial game using the 3D graphical engine are used as training data. In order to verify the performance of the proposed methodology, a case study of real maritime accident situation was conducted. The performance of the proposed methodology outperforms original deep convolutional neural networks.

파낙사디올의 가토적출부신의 카테콜아민 분비작용에 관한 연구

임동윤(Dong Yoon Lim),박규백(Kyu Baik Park),김규형(Kyu Hyeong Kim),최철희(Cheol Hee Choi),배재웅(Jae Woong Bae),김무원(Moo Won Kim) 대한약리학회 1988 대한약리학잡지 Vol.24 No.1

본 연구에서 한국산 인삼의 활성성분의 하나인 Panaxadiol(PD)에 대한 가토적출부신에서 카테콜아민(CA)의 분비작용과 작용기전을 파악하고자 실험을 시행하여 다음과 같은 결과를 얻었다.PD(400μg)을 가토적출부신에 투여하였을 때 카테콜아민의 분비를 의의있게 증가시켰다. PD의 이러한 CA 분비작용은 atropine 처치로 현저히 억제되었다. Physostigmine 전처치시 PD 뿐만 아니라 Ach의 CA 분비작용은 뚜렷이 증가되었다. 그러나 chlorisondamine 전처치로 PD나 Ach의 분비 효과는 억제되었다. 또한 PD(400μg/30 min)을 주입한 후에 Ach의 CA 분비 효과는 오히려 강화되었다. PD나 Ach의 작용은 adenosine 전처러시 현저히 증강되었다. EGTA(5mM)와 함께 Ca-free Krebs액으로 30분 주입한 경우에 Ach의 분비작용은 거의 전적으로 차단되었으며, PD의 작용도 약화되었다. 이상의 실험결과로 보아, PD는 가토적출부신에서 Ca⁺⁺ 의존적으로 CA분비를 증가시키며, 이러한 작용은 cholinergi muscarinic 및 nicotinic receptor의 흥분작용에 기인하며, chromaffincell 대한 일부 직접작용도 개재되어 나타나는 것으로 사료된다. The effect of Panaxadiol(PD), which is an active component of Korean Ginseng Saponins, on the secretion of catecholamines (CA) from the rabbit adrenal gland and its mode of action were investigated in the present study. PD(400μg) increased significantly the secretion of CA from the isolated perfused rabbit adrenal gland. PD-induced secretion of CA was reduced markedly by treatment of atropine, CA secretion induced by Ach or PD was potentiated significantly by physostigmine-treatment. Chlorisondamine did inhibit CA secretion of PD or Ach. Perfusion of PD(400μg) for 30 min enhanced the secretory activity of CA by Ach. Ouabain weakened the secretory response induced by PD but rather enhanced the response by Ach. Adenosine-treatment resulted in marked enhancement of CA secretion by PD or Ach, Pefusion with Ca²⁺-free Krebs containing EGTA (5 mM) for about 30 min totally blocked secretory effect induced by Ach and also weakened that by PD. From the above experimental results, it is suggested that PD causes secretion of catecholamines from the rabbit adrenal gland by a calcium-dependent exocytotic mechanism. The secretory effect of PD is due to the stimulation of cholinergic muscarinic and nicotinic receptors present in the adrenal gland and partly to a direct action on the chromaffin cell itself.

Stress Urinary Incontinence 에서 Tension - Free Vaginal Tape 수술 후 결과에 대한 고찰

박성근(Sueng Geun Park),백영철(Young Chel Back),소영환(Young Hwan So),갈철우(Cheal Wo Gal),최숙희(Sook Hee Choi),김유진(Yu Jin Kim),배재웅(Jae Woong Bae),김동영(Dong Young Kim) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.1

N/A Objective : The purpose of the study was to evaluate the safety and efficacy of tension-free vaginal tape (TVT) placement for surgical treatment of stress urinary incontinence (SUI) in women. Methods : A retrospective study of 38 cases with SUI underwent the TVT procedure underlying local anesthesia at Daedong and Sewoong Hospital from March 2000 to December 2000, followed up more than six months who included in the study. Result : Operation times ranged from 20 to 53 minutes with a mean of 37.5 minutes. The mean blood loss was 90 mL. One major complication was encountered -bladder perforation (2.5%) and urinary retention was developed in 4 cases (10.5%). Length of hospital stay ranged from 0.8 to 11.3 days with a mean of 1.8 days. On postoperative follow-up from 6.1 to 17.5 months, thirty-three patients (87%) were cured, four (10.5%) were significantly improved and one was failed (2.5%). Conculsion : Althouth the follow-up period was short, the TVT procedure seemed to be a safe and effective method for the treatment of stress urinary incontience.

O,p'-DDD가 흰쥐의 신장에 미치는 독성에 관한 면역조직화학적 및 초미세형태학적 연구

배재웅,최종범,임성철,김균홍,기근홍,박동수,전호종,박규호 朝鮮大學校 附設 醫學硏究所 1989 The Medical Journal of Chosun University Vol.14 No.2

o,P'-DDD, a derivative of insecticide DDT, which reveals the toxicity and adrenostatic effet by cortisol biosynthesis in the zona fasciculata of adrenal cortex has been used for the treatment of unoperable adrenocortical carcinoma. Adverse effects of o,P'-DDD were usually dose-related and reversible. Most untoward effects include gastrointestinal disturbances, neuromuscular disorders, depression of central nervous system and skin rash but there is a few reports on its nephrotoxicities. o,P'-DDD in corn oil was administered orally to rats for 28 days to investigate its nephrotoxicity by observation of the ultrastructural changes and immunoglobulin deposition in renal glomeuli. The results obtained were as follows. 1. The ultimate destructive target of intracytoplasmic organelle in endothelium and epithelium of renal glomeruli was mitochondria. The severity of the mitochondrial alterations was dependent of the dosages of o,p'-DDD. 2. The characteristic ultrastructural changes in endothelium of glomerular capillary were swelling and detachment from underlying basement membrane. The splitting and complete separations between endothelium and basement membrane were observed in more advanced lesions by the increased dosage of administration of o,p'-DDD and basement membrane in capillary wall of renal glomeruli was irregular and partly thickened. 3. Amorphous double ring formation, electron dense deposits and multiple myelin figures in mitochondria were characteristic ultrastructural findings in the epithelium of glomeruli. 4. Ig G and Ig M depositions were clearly observed in the capillary lumen, subendothelial area and epithelial cells of proximal convoluted tubule. These depositions were more prominent by the increased of o,p'-DDD dosage. From the above results it is suggested that o,P'-DDD exhibits nephrotoxicity by mitochondrial destruction in epithelium and endothelium of glomerular capillary and the severity of mitochondrial alterations was dose dependent of o,p'-DDD.

우리나라 老年人口의 死因構造에 관한 硏究

裵楨燁,裵在雄,金武源,金文石,黃斗煥,李章熙,金鍾石,鄭耀翰 최신의학사 1988 最新醫學 Vol.31 No.9

肝疾患死亡에 관한 硏究

姜東燮,裵在雄,金武源,金文石,黃斗煥,李章熙,金鍾石,文宰奎 최신의학사 1988 最新醫學 Vol.31 No.9