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유자영,양시용,송민동 建國大學校 自然科學硏究所 2002 建國自然科學硏究誌 Vol.13 No.1
본 연구는 가축의 장내에서 유익한 영향을 주는 유산균을 돼지의 분변으로부터 분리하고 내산성과 내담즙성이 뛰어난 균을 선별하여 유산균 생균체를 준비하고자 실시하였다. 분변에 섞여 있는 다른 균들과 산이나 담즙액에 약한 균들은 처음부터 분리하기 위하여 pH를 2.0으로 맞춘 MRS broth에 배양시키고 oxgall이 첨가된 배지에 도말하여 40개의 균주를 분리하였다. BCP배지를 이용해서 유산의 생성여부를 확인하고, O.D값과 pH를 측정하였으며, 그람염색과 형태관찰을 통하여 33개의 균주를 분리하였다. 분리된 균주중에서 산에 대한 내성이 있다고 판단되는 12개의 균주를 분리해냈고, 12개의 균주의 내산성 및 내담즙성을 3회에 걸쳐 시험하고 내산성과 내담즙성이 우수한 3개의 균주를 선별하였다. 마지막으로 담즙산 농도별 시험을 행함으로써 가축의 장까지 살아서 도달하기 위해 꼭 갖추어야 할 조건인 내산성과 내담즙성이 가장 좋은 #32-1 균주를 최종 선발하였다. 유산균이 생균제로서의 기능을 수해하려면 oxgall이 0.3% 함유된 배지에서 성장할 수 있을 정도의 내성을 갖고 있어야 한다는 보고가 있는데 #32-1 균주의 경우 담즙액의 농도가 3%인 배양액에서도 높은 생존률을 보임으로써 생균제로서 사용하기위해 갖고 있어야 할 담즙에 대한 내성이 우수하다고 판단하였다. This study was performed to screen lactic acid bacteria for the direct-fed-microbials. A lactic acid bacteria which has resistant to acid and bile salts were isolated from swine feces. Among the previously obtained acid tolerant strains, three of them show resistant to artifical gastric juice and bile. Finally, 321- stain was selected for good growth an high survival rate. The finally selected 32-1 stain was identified as Lactobacillus salivarius. The strain, which named L. salivarius JY 32-1, was able to tolerate acid condition down to pH 2.5 and could also tolerate bile at 3%.
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金永基,金明基,金淑子,劉夏榮 명지대학교 대학원 1998 대학원논문집 Vol.2 No.-
Whether Korean peninsular's dividing cause from the dividing of 38 degree line according to the "General order No 1" in 1945 or from the "Armistice Agreement" in 1953, that was not our will but outside's power. We can not say that reunification of our country would depend on foreign power because its dividing caused by outside power. Enter-Korea have been hostilize each other regarding as irregular group under dividing seatus. It is certain that our facing problem and ultimate objective is reunification not the naturalization of dividing. Accordingly, we have to consider sincerely reunification of our country considering enough inner or outer conditions given to us. Not only South Korea but North have a ability to take a reunification treaty with domestic law and international. The problem of future is when and how we will have to pursuit discussion first procedure. Our country require earnestly our wisidom and wit than ever for reunification. The responsible offiser of reunification policy have to decide sincerely reunification policy according to the high opinion of the export and many people working every parts.
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구자갑,김운학,최정호,유영화,정양규 한경대학교 2004 論文集 Vol.26 No.1
This paper study on soil properties and earth pressure at the domestic multi layer. Reliable predictions of the movement of earth retaining structures and the ground adjacent to the braced walls in urban excavation are often difficult due to many variable factors. Also, Unpredictable behavior of ground and retaining structure due to variation of the above factors may cause considerable damage to the adjacent structures, and cost many of human lives as a results of retaining wall failure. The earth pressure at the retaining walls was measured at total 13 numbers sites and Peck's empirical earth pressures adapted at the design stage. The geotechnical properties by this research was proposed as a preliminary design guide line in urban excavation where controling ground movement and safety assurance of adjacent structure. The earth pressure of rock masses were found to be 70.14% of Peck's earth pressure and the earth pressure of soil were found to be 44.4% of Peck's earth pressure. It is indicated that the design earth pressures of a retaining wall is
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노유자,김춘길,이영숙 성인간호학회 1999 성인간호학회지 Vol.11 No.4
This study was done to analyze research trends and to suggest future directions for nursing research on the quality of life (QL) in Korea. The purposes of this study were to describe systematically 10 years of QL nursing, and to identify patterns of tools and significant variables on studies of QL. This article reviewed 71 nursing researches on QL between 1987 and 1996, by examining them according to the period of publication, research for a degree or not, research design, type of subjects, dat collection methods, measurement instruments and range of reliabilities, type of nursing intervention and association of QL and related variables. The findings of the analysis can be summarized as follows: 1. The number of studies related to QL increased from 1987 to 1994. But they have decreased since 1995. 48 of them(67.6%) identified surveys and correlational studies. 2. In all the titles of the studies, there were 7 types of concepts, including QL, quality satisfaction, life satisfaction, and well-being. Among them, QL was the most often used. 3. The subjects of 21 studies were healthy people. In 49 studies, patients had various illnesses. The majority of the patients were hemodialysis patients and cancer patients. Questionnaires were used for data collection in 58 studies. 56 studies were done for the thesis of a degree. while those in the remaining studies were not for degrees. 4. The research designs were various, except in the thesis for a doctorate. There were many more experimental studies(57.1%) done in the thesis for a doctorate. 5. The types of intervention were categorized as exercise(6), supportive care(3), reminiscence(2), relaxation(1), music(1), and hospice care(1). 6. As measurement tools, 14 types of tools relating to QL were used. 11 of them were composed of multiple dimensions. Among them, the QL Scale by You-Ja Ro(1988) was the most widely used in the studies. In the following, the Life Satisfaction Index by Jin Yun(1982), the Quality of life Scale of National Conference of Cancer nursing(1983), and the Life Satisfaction Index by Wood, Wylie & Sheafor(1969) were used. The reliabilities of the QL Scale were reported in 63 studies, and the Cronbach's alpha coefficients were over 0.7 in 60 cases. The quality of life scales included five dimensions ; such as physical, psychological, social, economical, and spiritual dimensions. 7. There were two categories of factors influencing the quality of life: First, factors that cannot be changed by nursing intervention were : age, educational level, gender, marital status, income, employment status, occupation, number of family members, religion, and illness history. Second, factors that can be changed by nursing intervention were : physical activity, health status, the presence of complications, support, self-esteem, stress, self-efficacy, activities of daily life, physical strength, satisfaction with life, and anxiety. Types of nursing intervention used to improve quality of life were : therapies in relaxation, exercise, reminiscence, and family support, social support, and hospice care. On the basis of the above findings, the following recommendations were made : 1. Further studies on the quality of life instruments of analysis are needed to provide an understanding of dimensions of life in detail. 2. A Meta-analysis needs to be conducted to identify intervention effects on the quality of life. 3. A tool development study is needed to measure the quality of life appropriately in different illnesses. 4. More replicated analysis studies of QL are needed to prove the effect of variables on the QL.
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임영자,박유신 복식문화학회 2000 服飾文化硏究 Vol.8 No.5
The purpose of this study is to discover the interrelationship between modern costumes and Seung-Hee Choi's dancing costumes in 1930s. Seung-Hee Choi, who was a dancing pioneer in Korea, introduced the modern dancing costumes for the first time in Korea. The results are as followed; First, we can find Eroticism in Choi's dancing dress. Choi's dancing costume had used gold thread, silver threads spangle, see-through and a half-naked body clothing that decorated with beads and cords. It is similar to modern costumes in ubjet mitter, silhouette, design, and so on, Second, the influence of Futurism was in her dancing costumes, that is, it represented asymmetry designs and rectilinear figures. Third, not only beauty but also a Functionalism was expressed in her dancing costumes. Functional beauty was shown through bias cut, pleats, slit, and so on.
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자매에서 발생한 중증 마이코플라즈마 폐렴에서 스테로이드 치료
김도희,이경일,김명숙,윤유숙,황자영,임정우,강진한,이준성 대한감염학회 2009 감염과 화학요법 Vol.41 No.3
Numerous evidences support the pathogenesis that M. pneumonias pneumonia is associated with cell-mediat-ed immune reaction. We report 2 cases of severe M. pneumoniae pneumonia in previously healthy sisters, who were both admitted during an epidemic of M. pneumoniae pneumonia. The elder sister, who was 16 years old, was admitted with M. pneumoniae pneumonia. She showed no response to treatment with clarithroycin and levo-floxacin, and eventually progressed to severe ARDS, requiring mechanical ventilation. After treatment with hydrocortisone (200 mg/day), there were rapid improvements in clinical manifestations and chest radiographic findings. The younger sister, who was 14 years old, was admitted 10 days later, Presenting with fever but no pneumonic lesions on chest radiograph. Just like her sister, the infection showed no response to clarithromycin. Fever persisted and pneumonic consolidation with mild pleural effusion was noticed in the left lower lobe on the 5th hospital-day. After treatment with oral prednisolone (1 mg/kg), she showed rapid defervescence and on the 8th hospital day, no pneumonic lesions were detectable on chest radiograph. Given the fact that the pathogenesis of pneumonia and extrapulmonary manifestations in M. pneumoniae infection is immune-mediated, an immuno-suppressive therapy would be validated for selected patients with M. pneumoniae infections.
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Na Young Jin,Mi Jin Jeon,You Kyoung Lee,San Na Oh,Mi Ja Seo,Young Nam Youn,Yong Man Yu 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04
Bacillus thuringiensis (Bt) is a gram-positive bacterium that produces parasporal crystal proteins known as endotoxins or Cry proteins. The Cry protoxins are then cleaved by insect midgut proteinases to form active Bt toxins. The activated Cry protein then binds to specific receptors at the midgut epithelium. Cadherin-like and aminopeptidase N (APN) proteins are involved in Bt toxin binding by interacting sequentially with different toxin structures. Aminopeptidase N (APNs) from several insect species have been shown to be putative receptors for these toxins. We have characterized four different midgut APNs(APN1, APN2, APN3, APN4) cDNAs from S. exigua. Forward primers and reverse primers for confirmation of four different midgut APNs were designed based on their sequences cloned from the cDNA libraries. Quantitative RT-PCR procedures includes 42℃ for 20min (cDNA synthesis), 99℃ for 5min, and 35 cycles (94℃ for 1min, and 60℃ for 50 s) for collection. Four aminopeptidase N isoforms were confirmed with qRT-PCR. Sequence analysis was performed by BlastX search the National Center for Biotechnology Information(NCBI) nucleotide. Furthermore, double-stranded RNAs(dsRNAs) were synthesized. DsRNAs were determined for bioassay.
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