衛生昆蟲에 대한 無公害農藥(昆蟲發育沮害劑)의 殺蟲機作 및 應用技術開發硏究

金正和,李炯來,李仁煥,李容揆,崔鐘錫 충북대학교 농업과학기술연구소 1993 農業科學硏究 Vol.10 No.2

In recently, much pesticides were used for control of agricultural and medical insects. The result of using pesticides were social problem because of environmental pollutions with pesticides in urban and farm village. The environmental pollutions were very serious. This experiment was conducted to eluciated of reduce to environmental pollution bring about using pesticides (organophosphates, carbamates, pyrethroids, and insect growth regulators) for control of medical insects(house fly, Musca domestica L and mosquito, culex pipiens pallens copuillett) and carried out the method of pest control. And the result are summarized as follows. The Kyongbuk house fly strain treated with pyriproxyfen on third instar larvae were very high mortality and low emergence. When use it, at the same time, we can reduce degree of environ-mental pollution and house fly populations. Using of imidan was effective in Seoul and Chungnam house fly strains. In mosquito larvae, the efficacy of icon to chungbuk strain, propoxur to Chungnam and Chungbuk strain, imidan to Kyongbuk strain, fenthion to Chungnam strain were excellent. In result of spraying imidan and icon on adult mosquitoes, the efficacy of icon was excellent than imidan. In esterase electrophoresis, third instar larvae of Chungnam house fly strain fed on artificial diet mixed with pyriproxyfen LC50 were very high activity of -Est-αl in early stage(second day after third instar larvae fed on diet), and the activity of Est-αl was very strong in late stage(se-vents day after third instar larvae fed on diet). In Kyongbuk strain, -Est-αl and Est-αl were detected in early and late stage, and activities of and -Est-αl and Est-αl were detected almost equally in late stage. It was very characteristic in this experiment. Third instar larvae fed on diet mixed with pyriproxyfen LC95 were detected -Est-αl and Est-αl almost equally. It was believed moulting control in insects. In electrophoresis with head, thorax, abdomen of house fly adult, -Est-α2 -Est-αl, Est-αl, Est-α2, Est-α3 were detected in abdomen in susceptible strain and only -Est-αl, was net detected in thorax -Est-αl, was detected in head, thorax and abdomen of Chungbuk strain and -Est-αl was detected in head, -Est-α2 in thorax of same strain. Activities of chitinase in late instar larvae of house flies fed on artificial diet mixed with pyrip-roxyfen LC50 and LC95, in pyriproxyfen LC95, analysised low and high again with the lapse of time in Kyongbuk strain. This was believed that the activity of chitinase was became high just before emergence. The result of filter paper test with mosquitoes were very high index(265) of esterase activity in Chungnam strain among all mosquito strains. In this reason, we can suppose that used pyrip-roxyfen (IGR) in Chungnam area for control pest. In this reason, it was more reasonable made use of pyriproxyfen than other pesticide in Chungnam area.

昆蟲의 酵素分析에 의한 環境汚染比較에 관한 硏究

金正和,李仁煥,李容揆 충북대학교 농업과학기술연구소 1992 農業科學硏究 Vol.10 No.1

The experiment was conducted to elucidate the differences of several mosquito strains(Culex pipiens pallans Coquillet : Mushimcheon, Daecheongdam, Oksan, Weolacksan) to various insecticides using the methods such as bioassay, electrophoresis, and filter paper test. And the results are summarized as follows. 1. In LC50, Mushimcheon strain showed the highest insecticidal resistance while that of Weolacksan strain was the lowest. Resistant ratio(RR50) of mosquito srains to temephos was very high while all strains showed very low rate to propoxur. In LC95, insecticidal resistance to temephos was very high in Oksan strain while Daecheongdam strain was very low. 2. Est-Bl was detected in all strains tested by electrophoresis. And the activity was very high in Oksan strain and very low in Daecheongdam strain. 3. Esterase activity, determined by filter paper test and digital densitometry, was very high in Oksan strain and that of Daecheongdam was very low. 4. The results obtained thus far indicated that the mosquito strains that inhabit in Oksan and Mushimcheon region where respective agricultural chemicals and insecticides to control medical insects are heavily applicated are more contaminated by insecticidal chemicals than other regions.

심장수술 시 농축적혈구, 자가수혈기 혈액, 체외 순환기 혈액에서의 유리 혈색소 농도 비교

송장호,구승우,백종화,정용보,이정순,최인철 대한마취과학회 2002 Korean Journal of Anesthesiology Vol.43 No.5

Background: Intraoperative autotransfusion or residual blood in a cardiopulmonaty bypass (CPB) circuit has been used to reduce the need for an homologous blood transfutsion during cardiac surgery. However, it may contain some free hemoglobin released from damaged cells. The load of blood containing free hemoglobin may cause renal dysfunction. We measured the amount of free hemoglobin in backed blood, cell saver blood and CPB blood to evaluate what is the least hemolytic blood transfused in cardiac surgery. Methods: This study was performed in 20 patients undergoing cardiac surgery. In each patient, the banked blood, intraoperative salvaged blood with a cell saver and CPB residual blood were sampled at the end of the operation. The concentration of free hemoglobin, hemoglobin and platelet counts were measured in these blood samples and percent of hemolysis was calculated (%hemolysis = [free hemoglobin] / .free hemogloben + hemoglobin]) Results: In salvaged blood with a cell saver, CPB residual blood and banked blood, hemoglobin concentrations were 20.1 ± 2.7 g/dl, 8.0 ± 1.1 g/dl, and 22.2 ± 2.7 g/dl, respectively. Free hemoglobin concentrations were 336.6 ± 239.5 mg/dl, 49.2 ± 26.8 mg/dl, and 279.5 ± 167.5 mg/dl respectively. Platelet counts were 26.1 ± 22.2 × 10^3/mm^3, 116.8 ± 56.5 × 10^3/mm^3, and 94.9 ± 43.6 × 10^3/mm^3 respectively. % Hemolysis were 1.6 ± 1.1%, 0.6 ± 0.4%, and 1.2 ± 0.7% respectively. In the comparison between the salvaged blood with a cell saver and CPB residual blood, free hemoglobin concentration, % hemolysis, and platelet counts had positive correlations (r = 0.8, 0.7, and 0.6). Conclusions: In twenty cardiac surgeries, CPB residual blood had a lower free hemoglobin level than the other two blood groups. The platelet counts in CPB residual blood were higher than those in cell saver blood but did not differ from those in banked blood. Therefore, CPB residual blood was the least hemolytic blood among the three blood groups when a transfusion was performed in cardiac surgery. (Korean J Acesthesiol 2002; 43: 588~593)

저공해농약(곤충발육저해제)의 주요 해충에 대한 살충실험

金正和,李炯來,金柄植,李仁煥 충북대학교 농업과학기술연구소 1993 農業科學硏究 Vol.11 No.1

The experiments were carried out on toxicological comparisons of the new insect growth regu- lators(IGR) against the fall webworm(Hyhamtria cunea) and the house fly(Musca domestica). The LC50 values of tebufenozide, chlorfluazuron and diflubenzuron against the Hyphantria cunea in the 3rd instar stage within the 1st generation collected in cheongju, were 6.0894, 7.7768 and 9.1322 ppm, respectively. In toxicological studies at the 0.5ppm level of chlorfluazuron and buprofezin against the different larval stages of Musca domestica, the ratio of pupation was 20%~35% at the 1st instars, but not shown at the 2nd and 3rd instars, while, the ratio of emergence showed the decreasing tendency at the different stages. In high doses(5, 25, 50, 500ppm) of chlorfluazuron and pyriprozyfen against the house fly in the 2nd instars, the ratio of pupation in chlorfluazuron showed the decreasing tendency at the high dose but pyriproxyfen did not. The ratios of emergence in two IGR showed 0% without regard to dose. In determination(LC50) of the tested IGR against the house fly in the 2nd instars, the ratio of pupation did not show any differences in all IGRs, but the ratio of emergence showed a decreasing tendency with the increasing doses. The LC50 values of pyriproxyfen, chlorfluazuron and buprofezin were 0.0527, 0.0898 and 13.7669 ppm, respectively.

대뇌의 방사선조사후 괴사 1예

노순성,서중근,주정화,이훈갑,김인선 대한신경외과학회 1978 Journal of Korean neurosurgical society Vol.7 No.2

A case of postirradiation necrosis of the brain, clinically simulating neoplasm, is reported. A 29 years old male underwent a surgery with a partial removal of chromophobe adenoma of the pituitary gland postoperatively received deep radiation therapy with CO^(60). A total dose of 4,500 r. was given in 35 days. Approximately six months after the irradiation, the patient became confused and complained of frontal headache in association with signs of increased intracranial pressure and of intracranial dynamic mass lesion. Cerebral angiography and C-T scanning demonstrated findings of a large mass in the right temporal region. At operation done nine months after his initial craniotomy, tissue of the mass appeared to be a glioma and large blocks of tissue were resected. Histologically it was verified to be necrosis of the brain.

정상과 갑상선 종양조직에서 사람 IGF-I 유전자의 발현

김성운,장현하,박상미,김덕윤,우정택,양인명,김진우,김영설,김광원,고석환,홍성화,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

Many of the growth-promoting properties of growth hormone(GH) are mediated by insulin-like growth factor-I(IGF-I), a highly conserved circulating 70-amino acid peptide. Recent studies have shown that multiple mechanisms influence IGF-I gene expression, including transcription from two promoters, alternative RNA splicing, and variable polyadenylation. In thyroid tissue, thyroid stimulating hormone(TSH) and IGF-I are the most possible candidates for follicular cell proliferation and hypertrophy. Actually IGF-I had autocrine and paracrine effect for tissue growing. We prepared thyroid tumor tissue mRNAs using single step method for detecting IGF-I levels according to different tissues, i.e., thyroid adenoma or papillary thyroid carcinoma. We used Northern blot analysis for IGF-I mRNA and RNase protection assay (RPA) for IGF-I transcription start sites. For Northern blot, we used whole human IGF-I cDNA as a DNA probe and for RPA, we used IGF-I exon 1 containing noncoding promoter 1 as a riboprobe. We got good RNA bands from Northern blot analysis around 1 kb (IGF-IA) and 7.5 kb (IGF-IB) region. To clarify the amount of both IGF-IA and IB mRNAs, we measured autoradiographied signal of IGF-I mRNAs bands using densitometer. In IGF-IA signals, there's no change among liver and thyroid tissues, but in case of IGF-IB mRNA bands, the signal was markedly increased in thyroid carcinoma tissues than that of normal thyroid tissue (85% vs 14%). In the study of RPA, all thyroid tissues used the same transcription start sites as those of liver's. We concluded that that this different regulation of IGF-I mRNA was originated from tissue specificity. That meant some tissue specific transcription factor/s were related to tissue IGF-I expression.

고위험군의 원발성 전신성 유전분증 1례 보고 및 조혈모세포이식에 대한 문헌고찰

심준,박수정,엄현석,김기영,박은정,강인중,조병식,이안희,한치화 대한조혈모세포이식학회 2001 대한조혈모세포이식학회지 Vol.6 No.2

저자 등은 클론성 형질세포질환과 동반된 젊은 연령의 원발성 전신성 유전분증 환자를 진단하였기에 다발성 골수종과의 감별 진단, 치료 , 예후 및 고용량 항암화학요법과 조혈모세포이식에 관하여 문헌고찰과 함께 보고하는 바이다. Primary systemic amyloidosis (AL) is a rapidly fatal disorder related to plasma cell dyscrasia. Conventional dose of melphalan, which prolongs the duration of survival by about 10 months, does not improve the functions of impaired organs in most cases. The high dose chemotherapy followed by autologous hematopoietic stem cell rescue for AL, in spite of its high treatment-related mortality, is a new approach to achieve high response rate and better survival. We experienced a 35-year old man with AL(involving heart, liver, stomach, kidneys, peripheral nerve, and rectum) who did not respond to the standard schedule of melphalan plus prednisone and had rapidly fatal course with organ failure. Hence, we evaluate its availability by reviewing the recent reports of high dose chemotherapy in AL.

INHIBITION OF EPITHELIAL-MESENCHYMAL TRANSITION (EMT) BY IN-1130, A SMALL MOLECULAR INHIBITOR OF TRANSFORMING GROWTH FACTOR-β TYPE I RECEPTOR KINASE

In-Hye Kwon,Bo-Mi Kang,So-Jung Moon,Jee-Yeon Son,Eun-Jeong Yoon,Jung-Wha Moon,Chul-Yong Park,Dae-Kee Kim,Yhun-Yhong Sheen 한국환경독성학회 2009 한국독성학회 심포지움 및 학술발표회 Vol.2009 No.11

Western-style diets induce macrophage infiltration and contribute to colitis-associated carcinogenesis

Kim, In-Wha,Myung, Seung-Jae,Do, Mi Young,Ryu, Yeon-Mi,Kim, Mi Jung,Do, Eun-Ju,Park, Sehan,Yoon, Soon Man,Ye, Byong Duk,Byeon, Jeong-Sik,Yang, Suk-Kyun,Kim, Jin-Ho Blackwell Publishing Asia 2010 Journal of gastroenterology and hepatology Vol.25 No.11

<P>Abstract</P><P>Background and Aim: </P><P>A Western-style diet (WD) is known to play an important role in inflammatory bowel disease and colon carcinogenesis. The purpose of this study was to understand the role of macrophages in WD-induced colitis associated with carcinogenesis.</P><P>Methods: </P><P>Male BALB/c mice were fed a WD or a control diet (CD) for 4 weeks and exposed to azoxymethane (AOM) followed by 2% dextran sulfate sodium (DSS) for 7 days.</P><P>Results: </P><P>The WD increased susceptibility to DSS-induced inflammation and accelerated the infiltration of macrophages. The incidence and multiplicity of colon tumors were higher in mice fed the WD than in those fed the CD (<I>P</I> < 0.05). Levels of prostaglandin-endoperoxide synthase (PTGS) 2 and prostaglandin (PG) E<SUB>2</SUB> in the colon were higher after treatment with AOM and DSS in mice fed the WD than in those fed the CD. In addition, WD consumption increased the DNA binding activity of nuclear factor-kappaB and the serum concentration of tumor necrosis factor (TNF)-&agr;. Mice fed the WD had higher numbers of F4/80-positive cells surrounding cancer cells compared with mice fed the CD. These cells expressed PTGS2, TNF-&agr; and &bgr;-catenin, which are up-regulated by the WD. We also found that the WD increased unphosphorylated &bgr;-catenin accumulation in the cytoplasm and nucleus of colon cancer cells.</P><P>Conclusions: </P><P>A WD increases the susceptibility to DSS-induced inflammation and accelerates the infiltration of macrophages. In turn, this resulted in the development and progression of colon cancer.</P>

Comparative in vivo biodistributions of nanoparticles and polymers of ¹⁷⁷lutetium-labeled hyaluronic acids in mice during 28 days

Lin, Chunmei,Jeong, Ju-Yeon,Yon, Jung-Min,Park, Seul Gi,Gwon, Lee Wha,Lee, Jong-Geol,Baek, In-Jeoung,Nahm, Sang-Soep,Lee, Beom Jun,Yun, Young Won,Nam, Sang-Yoon The Korean Society of Veterinary Science 2017 大韓獸醫學會誌 Vol.57 No.2

Hyaluronic acid (HA) has been investigated for biomedical and pharmaceutical applications. This study was conducted to determine the distributions of HA nanoparticles (NPs; size 350-400 nm) and larger HA polymers in mice at intervals after application. $^{177}Lutetium$ (Lu)-labeled HA-NPs or HA polymers were intravenously injected (5 mg/kg) into male ICR mice, and radioactivity levels in blood and target organs were measured from 0.25 h to 28 days post-injection. In blood, the radioactivities of HA-NPs and HA polymer peaked at 0.5 h after injection but were remarkably decreased at 2 h; subsequently, they maintained a constant level until 6 days post-injection. HA-NPs and HA polymers were observed in the liver, spleen, lung, kidney, and heart (in ascending order) but were seldom observed in other organs. After 3 days, both the HA-NP and HA polymer levels showed similar steady decreases in lung, kidney, and heart. However, in liver and spleen, the HA-NP levels tended to decrease gradually after 1 day and both were very low after 14 days, whereas the HA polymer level accumulated for 28 days. The results indicate that HA-NPs, with their faster clearance pattern, may act as a better drug delivery system than HA polymers, especially in the liver and spleen.