HMG-CoA 환원효소 억제제에 의한 ICAM-1 유전자의 발현조절

김현진,정효균,홍우정,김군순,조영석,김도희,채수흥,구본정,송민호,노흥규,김영건 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Background : ICAM-1 act as one of major adhesion molecules in the atherosclerotic lesion. ICAM-1 expression is mainly regulated at the level of transcription and depend on IFN-γ signal transduction pathway in which the STAT1 transcrption factor is a critical intermediate. IFN-γreceptor not only initiates tyrosine 701 phosphorylation of STAT1 by Jak1 and Jak2, but also phosphorylates serine 727 through the activation of Raf-1/MAP kinases. HMG-CoA reductase inhibitors have anti-atherosclertic effects, beyond normalization of hypercholesterolemia, by directly acting on endothelial cells, macrophages and vascular smooth muscle cells. HMG-CoA reductase inhibitors suppress the synthesis of isoprenoid intermediates such as geranylgeranyl-pyrophosphate or farnesylpyrophosphate. These effects results inhibitors suppress the synthesis of isoprenoid intermediates such as geranylgeranyl-pyrophosphate or farnesylpyrophosphate. These effects results inhibition posttranslational farnesylation and geranyl-geranylation processing of small GTP-binding preoteins and inhibition of normal signaling activities. Method : We made several 5'-deletion constructs of rat ICAM-1 promoter and analyzed the promoter activities by measuring the luciferase activity after transfection into ECV304 cells and smooth muscle cells. We checked the level of total and phosphorylated STAT1 protein by immunoblot analysis using specific antibodies. Results : Lovastatin inhibits IFN-γ-induced ICAM-1 gene expression in the ECV304cell. The cells pretreated with PD98059, MEKK inhibitor showed significantly low ICAM-1 RNA induction with IFN-γ stimulatio. IFN-γ induced phosphorylation of tyrosine 701 was not significantly changed by the pretreatment of lovastatin. But lovastatin suppresses IFN-γ-induced phosphorylation of ERK1/ERK2 which are responsible for the seine 727 phosphorylation in STAT1. Conclusion : We showed that HMG-CoA reductase inhibitors, lovastatin, suppresses IFN-γ mediated ICAM-1 gene expression through the inhibition of transcription. HMG-CoA reductase inhibitor suppresses IFN-γ-induced phosphorylation of serine 727 in STAT1 through the modulation of MAP kinases.

Molecular analysis of TMC1 gene in the Korean patients with nonsyndromic hearing loss

Hyo‐Kyeong Kim,Yee Hyuk Kim,사공보름,권태준,오세경,Hye‐Jin Lee,Kyu‐Yup Lee,이상흔,김언경 한국유전학회 2011 Genes & Genomics Vol.33 No.2

Hereditary nonsyndromic hearing loss (NSHL) is a highly heterogeneous disorder in humans. Mutations of the transmembrane channel‐like (TMC1) gene have been identified as the genetic cause for both autosomal recessive (DFNB7/11)and autosomal dominant (DFNA36) nonsyndromic hearing loss. To evaluate the spectrum and frequency of mutation(s)caused by TMC1 gene in the Korean population, we have performed sequencing analysis of the PCR products amplified from genomic DNA of each proband in 193 unrelated families showing 30 autosomal dominant and 163 autosomal recessive inheritance patterns. As a result, we identified eight different novel sequence variations for the first time in this study,respectively. However, none of these showed co‐segregation of phenotype in the families. Therefore, our study suggests that the TMC1 gene is not the cause of nonsyndromic hearing loss in the Korean population.

구개부에 발생한 다형성선종의 치험례

이창진,김태열,김효언,김희광,전인성,윤규호 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.2

Pleomorphic adenoma is the most common of all salivary gland tumors, constituting over 50 per cent of all cases of tumors, and approximately 90 per cent of all benign salivary gland tumors. Since the term mixed tumor' was introduced by Broca for its dual origin of epithelial and mesenchymal elements, the term plemorphic adenoma suggested by Willis characterizes closely the unusual histologic pattern of the lesion. The parotid gland is the most common site but it may occur in any of the salivary glands. It is somewhat more frequent in women and in the fourth to sixth decades, but they are also relatively common in young adult and have been known to occur in children. Treatment of choice is surgical excision. Adequate surgery with safe margin reduce its recurrence rate. We represent a case of pleomorphic adenoma with literature review in 65-year old male occured in the palate. The lesion was successfully treated by surgical excision.

Mild Cognitive Impairment 노인군의 점화효과 특성

박재우,김도관,고선규,고효정,김성환,김재경,서민영,이인수,김지혜 大韓神經精神醫學會 2002 신경정신의학 Vol.41 No.3

연구목적 : 이 연구에서는 K-MMSE에서 25점 이하를 받은 50대 이상 노인들을 MCI(Mild Cognitive Impairment) 군으로 보고 이들의 인지 기능 특성을 점화 효과를 중심으로 살펴보고자 하였다. 먼저, 점화 효과가 MCI 노인군에서 있어서도 유지되고 있는지를 알아보고, 인지 기능의 손상 정도에 따라 점화 효과가 유의미하게 변화하는 지를 살펴보고자 하였다. 방 법 : 본 연구에서는 K-NAME 점수 25점 이하의 노인 96명으 대상으로 ADAS-Cog와 일종의 간접 기억 검사 도구인 지각적 파악 과제를 제작하여 점화 효과를 측정하여 인지 기능의 퇴행이 시작된 고연령군이 나타내는 암묵 기억 능력 특성을 알아보았다. 점화효과는 컴퓨터 상에 사전 노출된 자극과 그렇지 않은 자극을 순간 노출시켜 미리 노출된 자극에 대해 반응 시간이 줄어들고 정확 반응이 증가하는 정도로써 측정하였다. 이 때 지능이나 연령, 학력 수준과 같은 변인이 미치는 영향을 통제하기 위해 한국판 웩슬러 지능 검사의 <어휘>, <토막> 소검사를 실시하였고, 인구통계학적 변인에 관한 면접을 실시하였다. 통계적 분석 과정에서는 먼저, 연구에서 측정된 점화효과의 특성을 알아보기 점화 효과 측정치의 기술 통계치를 구하였으며 다른 측정 변인들과의 상관 분석을 실시하였다. 마지막으로 측정된 점화효과가 인지 기능의 퇴행 수준에 비례하는 지의 여부를 파악하기 위해 ADAS-Cog 및 나이, 교육 연한 및 지능 수준을 독립변인으로 회귀분석을 실시하였다. 결 과 : 먼저 기술 통계 분석 결과에 의하면 본 연구에 참여한 피보험자들의 경우 점화효과에 의해 미리 노출된 자극을 평균 3.6개 더 정확히 파악하였으며 평균 59ms 더 빠르게 반응한 것으로 나타나 이들 집단에서도 점화효과가 유지되고 있음을 알 수 있었다. 다음으로 변인들 간의 상관 분석 결과에 따르면, 정확 파악 반응 점수에 따른 점화효과는 전반적인 인지 기능을 평가하는 K-MMSE 및 ADAS-Cog와 정적인 상관이 있는 것으로 나타났으며 반응 속도로 측정한 점화효과의 경우에는 나이와는 정적인 상관이, KWIS의 <어휘> 소검사 점수와는 부적인 상관이 있는 것으로 나타났다. 또한, 본 연구에서 사용된 점화 효과 측정치를 종속 변인으로 ADAS-Cog와 KWIS의 어휘 및 코막 소검사로 측정한 지능 수준, 나이와 교육 연한과 같은 인구 통계학적 변인을 독립 변인으로 회귀 분석을 실시한 결과에 따르면 정화간응 점수로 측정한 점화 효과의 경우 상관 분석 결과에서와 같이 ADAS-Cog 변인만이 유의미한 효과를 갖는 것으로 나타났으며 반응 속도로 측정한 점화 효과의 경우 나이의 효과만 유의미한 것으로 나타났다. 결 론 : 이러한 결과는 경미한 인지 기능의 손상이 진행되고 있는 고연령군의 경우에는 외현 기억 능력은 손상이 시작되지만 점화효과로 측정된 암묵 기억이 전반적으로는 유지되다가 인지 기능의 퇴행이 진행됨에 따라 노출된 자극을 더 정확히 파악하는 능력이 저하되고 점화 효과에 의해 인출 속도가 빨라지는 현상이 연령이 증가함에 따라 함께 증가하는 것으로 나타남을 말해 준다. Objectives : In this study, the cognitive characteristics, especially priming effect, of above 50 years old age group who had less than 25 points in K-MMSE were investigated. Methods : In this study, an implicit memory test measuring priming effect was made and administered ti old age people who had less than 25 points in k-MMSe in screening session. To control the effect of age, educational level and intelligence, demograohic variables were measured, and the subjects of KWIS, vocabulary and block design were administered. Descriptive statistical analysis of the two priming measures and correlational analysis between variables were done. To test the effect of cognitive functioning on priming effect multiple regression analysis was done. Results : Correlational analysis revealed priming score obtained from correct identification response was positively correlated with K-MMSE and ADAS-Cog. And priming score obtained from mean reaction time was positively correlated with age and negatively correlated with vocabulary substest of KWIS. The regression analysis results indicated from correct identification response, whereas age has significant effect on priming score obtained from mean reaction time. Conclusions : The results revealed that as cognitive deterioration progresses priming effect which identify primed stimulus correctly diminish, and as one grow older within age 50 to 70, priming effect which identifies primed stimulus quickly increase.

제 3형 Gaucher세병으로 인한 진행성 근간대성 간질 2예

허경,예종수,김상진,김성은,김응규,조효근,김찬환,김현주 대한신경과학회 1999 대한신경과학회지 Vol.17 No.3

상악 전치부에 발생한 낭종성 치아종의 치험례

김효언,윤규호,전인성,신재명,한광홍,배정호 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.1

Odontomas are odontogenic harmatomas which are composed of hard dental tissue. This lesion is composed of more than one type of tissue and, for this reason, has been called a composite odontoma. Odontogenic cysts are derived from epithelium associated with the development of the dental apparatus. Dentigerous cyst originated after the crown has been completely formed by accumulation of fluid between the reduced enamel epithelium and the crown. It also associated with crown of impacted tooth. Cystic odontomas are closely similar to dentigerous cysts clinically and radiologically. Treatment of choice is total removal(enucleation) or decompression(marsupialization). We represent two cases of Cystic odontomas with literature review in 69-year old male and 55-year old female occured in anterior maxilla. The leisions were successfully treated by marsupializaion.

상운동원성 질환에서의 신경전도속도 검사에 대한 고찰

김응규,김성은,조효근 인제대학교 1998 仁濟醫學 Vol.19 No.1

신경전도속도 검사는 말초신경의 이상 유무를 알아볼 수 있는 중요한 검사 방법 중 하나이다. 그러나 상운동원성 질환 환자에서도 이상을 보일 수도 있다는 보고가 있기에 과연 상운동원 성 질환에서 신경전도속도 검사가 이상을 보이는지, 이상을 보이면 어떠한 인자와 연관이 있는지 연관성을 알아보기 위하여 연구하였다. Background and Purpose : Neve conduction velocity(NCV) is a useful method for lower motor neuron disorders, especially in the peripherial neuropathy. But some authors reported that NCV was abnormal in the user motor neuron disorders. We conducted this study to see whether NCV abnormality was indeed present in the upper motor neuron disorders, and to see if present, what kinds of factors are associated. Methods : This study was done on 26 patients from Feb.1996 to Aug.1996 who admitted to the Busan paik hospital. Inje university with upper motor neuron disorders. Exclusion criteria was peripheral neuropathy, alcoholism and radiculopathy. NCV test was performed in the 4 extremities with F-wave latency, muscle power and atrophy. Results : The association between muscle power and atrophy existed only in the right upper extremity, that is, less muscle power, more atrophy. The muscle power in the right upper and lower extremities were associated with right upper extremity NCV abnormality. The muscle power in the left lower extremity were associated with left upper extremity NCV abnormality. There was no correlation between muscle power and atrophy whether NCV is normal or abnormal. Conclusion : NCV test was a useful method in the lower motor neuron disorders. But this study revealed that abnormality of NCV test was present in the upper motor neuron disordes. So the recovery of motor function might be influenced by abnormal NCV.

면내 지진파에 대한 부지응답해석

김효건,최홍석,손영호,최광규 동아대학교 건설기술연구소 1999 硏究報告 Vol.23 No.2

The site responses of seismic waves were considered when in-plane P and SV waves are incident to alluvial deposit of arbitrary shape. Parametric variables such as the incident angle and the dimensionless frequency and the sheat wave velocity ratio and the mass density ratio between the half-space and the allvial deposit were selected. For numerical analysis, the inhomogeneous alluvial deposit was modeled by using 8-node isoparametric finite elements and the surrounding homogeneous linear elastic soil half-space was modeled by using 3-node isoparametric boundary elements. The two elements at the interface were coupled together with the equilibrium condition of the tractions and the compatibility condition of the displacements.

부비동단층촬영에서 관찰되는 만성부비동염 병변내 고밀도 병변

정승규,김효열,동헌종 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.2

정신분열병 환자의 KCNN3 유전자에서 나타나는 삼핵산 반복서열

김도관,임신원,고효정,서민영,손성은,이소영,황혜진,진동규,김병로 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.5

연구목적 : 연구자들은 NMDA 수용체의 channel protein 합성에 관여하는 KCNN3 유전자에서 나타나는 삼핵산 반복서열(triple nucleotide repeat, TNR) 확장이 과연 정신분열병의 발병에 기여하는지를 최근 개발된 TNR copy 수를 정량화 함으로써 알아보고자 하였다. 방 법 : 연구대상은 정신과 외래 및 입원 환자 중 정신분열병 환자 245명과 건강 의학센타를 방문한 정상 성인 116명을 각각 선발하였다. 정신분열병 환자들이 정상인에 비하여 불특정 CAG 삼핵산 반복 서열 확장에 차이가 나는지 repeat expansion detection(RED) 방법을 확인한 다음, 그것이 KCNN3이나 CTG18.1 유전자의 CAG 반복서열 증가에 의한 영향인지 분석하였다. 그리고 untranslated region에서 TNR 증가를 불특정하게 일으킬 수 있는 ERDA1의 CAG 삼핵산 반복서열의 영향을 검증함으로써 KCNN3나 CTG18.1 유전자가 정신분열병의 발병에 기여하는 바를 평가하였다. 결 과 : 정신분열병 환자는 정상인에 비하여 KCNN3 유전자 부위에서 TNR 확장이 현저한 longer allele이 빈번하게 관찰되었다. 즉, KCNN3 유전자 부위에서 CAG 삼핵산 반복이 19번 이상 확장되는 염색체가 신분열병 환자군에서는 73.3% 발견되는 데 비해서 정상인군에서는 65.1% 발견되어 유의한 차이가 있었다(p=0.029, Fisher's exact test). 이러한 차이는 가족력이 있는 정신분열병 환자군(79.7%)에서 가족력이 없는 환자군(70.8%)보다 더욱 현저하게 관찰되었다(p=0.003, Fisher's exact test). 음성형과 양성형 정신분열병 환자군 간에서의 차이는 관찰되지 않았다. CTG18.1 유전자 부위의 TNR 확장이나 불특정 부위 염색체 전체에서의 TNR 확장은 환자군과 정상 대조군 간에 차이가 없었다. 환자군과 대조군 모두에서 불특정 TNR 확장은 ERDA1의 전사와 유의하게 관련성이 있었다(각각 r=0.45, p<0.001 ; r=0.44, p<0.001). 하지만 KCNN3 유전자 부위에서 TNR 확장은 ERDA1 score와 유의한 관련성을 보이지 않았다. 결 론 : 이상의 결과는 KCNN3 유전자가 한국인 정신분열병의, 특히 가족력이 있는 정신분열병의, 발병에 관여할 수 있음을 시사한다. 또한 ERDA1 전사는 불특정 TNR 확장의 원인으로 작용한다는 가설을 뒷받침한다. Objects : We investigated a possible association between the polymophic trinucleotide repeat(TNR) expansion in neuronal potassium channel gene KCNN3 and schizophrenia. Methods : CAG/CTG repeat distribution in KCNN3, CTG8.1 and ERDA1 was examined and the copy number of ligation product in repeat expansion detection(RED) was measured in Korean patients with schizophrenia(n=245) and ethnically matched healthy controls(n=116). Results : Longer alleles in the KCNN3 gene were over-represented in patients. The frequency of alleles with CAG repeats longer than 19 copy in the KCNN3 gene was higher in the patients with schizophrenia as compared to controls(73.3% vs 65.1% ; p=0.029, Fisher's exact test). And this difference was more prominent in schizophrenic patients with familial background(p=0.03, Fisher's exact test). We found no difference in the frequency of longer alleles between negative and positive subtypes of schizophrenia. Ligation product size in RED and alleles with CAG repeat number in the CTG18.1 gene was not increased in the patients. The copy number of ligation product in RED was highly correlated with CAG/CTG copies if ERDA1 in the patient group(r=0.45, p<0.001) as well as in the control group(r=0.44, p=<0.001). However, CAG repeat length in the KCNN3 gene was not correlated with ERDA1 score. Conclusions : Our results support the hypothesis that the longer allele of KCNN3 may be considered as a candidate gene for schizophrenia, especially in the case with familial background. And the RED assay results was affected by the CAG copy number of ERDA1.