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Genetic Variations in Korean Wild Ginsengs and Their Genetic Relationships
황혜진,연민지,성지연,박하영,김수옥,전형욱,김근종,최강준,이용진,서동상 한국유전학회 2006 Genes & Genomics Vol.28 No.2
Genetic diversity and genetic relationships were investigated in wild ginsengs collected from various natural habitats in South Korea using RAPD techniques. The UPGMA dendrogram established from the RAPD data demonstrated that the genetic diversity of wild ginsengs was more profound than that of cultivated variants. These differences in RAPD profiling were from their genomic sequence variation, supported by sequence analyses on the commonly amplified bands from wild and cultivated ginseng. Considering that ginseng is a self-pollinated and long-isolated fossil plant, which has not changed its shape appreciably throughout evolution, this diversity among ginseng plants grown in different locations and under different conditions was expected to some degree. Based on the sequence data, we conclude that one wild ginseng accession had been isolated for over 19 million years. In this report, we suggest that Korean wild ginseng has remarkable genetic diversity as compared with commercial Korean ginseng, and urge that measures be taken to conserve this endangered plant from reckless exploitation and possible extinction.
The Effect of Reader-based Variables on Incidental Vocabulary Learning Through Reading
황혜진 한국영어교육학회 2012 ENGLISH TEACHING(영어교육) Vol.67 No.4
This study investigated whether or not there is any difference in the extent to which incidental vocabulary learning takes place according to the presence of background knowledge. Along with background knowledge, three other variables such as reading comprehension ability, reading strategies, and knowledge of words were examined to determine how each variable contributes to incidental vocabulary learning. The data from two groups of 60 second grade students in a Korean middle school was analyzed in this research, and the followings were measured: the use of reading strategies,reading comprehension ability, knowledge of passage sight vocabulary, background knowledge on a text, and knowledge of target words. The findings revealed that the participants inferred the meaning of unknown words better when they had more background knowledge. Each of the four variables was a significant predictor of their incidental vocabulary learning and played a facilitative role in the lexical inferencing process. The results are discussed in terms of the conditions for efficient incidental vocabulary learning through reading.
Production of Liquiritigenin with Cell-based Biotransformation and Its Anti-Aging Activity
황혜진,정상철,박종필 한국생물공학회 2015 KSBB Journal Vol.30 No.4
In this study, an efficient whole cell-based biotransformation for the production of liquiritigenin was developed using Laetiporus sulphureus CS0218 as biocatalyst and aqueous extracts of Glycyrrhiza uralensis as co-substrate, respectively. In order to determine the efficacy of this method, the optimal bioconversion conditions including mycelial growth, three important enzyme activities (β-glucosidase, α-rhamnosidase and β-xylosidase), and apparent viscosity of culture broth were monitored. After optimization, aqueous extracts of G. uralensis were added to the culture medium to directly produce algycone liquiritigenin. By applying this strategy, 67.5% of liquiritin was converted to liquiritigenin at pH 3.0 after 9 days of incubation and finally liquiritigenin was purified from the reaction mixture. And then, their biological activities including anti-oxidant and superoxide dismutase were observed. In fact, purified liquiritigenin was capable of bi-directional functions (i.e., either up-regulation or down-regulation of SIRT1 which is associated with aging). The results indicate that this strategy would be beneficial to produce biologically active liquiritigenin and could be used in pharmaceutical, cosmetic and food applications.
Production of Liquiritigenin with Cell-based Biotransformation and Its Anti-Aging Activity
황혜진,정상철,박종필,Hwang, Hye Jin,Jeong, Sang Chul,Park, Jong Pil The Korean Society for Biotechnology and Bioengine 2015 KSBB Journal Vol.11 No.5
In this study, an efficient whole cell-based biotransformation for the production of liquiritigenin was developed using Laetiporus sulphureus CS0218 as biocatalyst and aqueous extracts of Glycyrrhiza uralensis as co-substrate, respectively. In order to determine the efficacy of this method, the optimal bioconversion conditions including mycelial growth, three important enzyme activities (${\beta}$-glucosidase, ${\alpha}$-rhamnosidase and ${\beta}$-xylosidase), and apparent viscosity of culture broth were monitored. After optimization, aqueous extracts of G. uralensis were added to the culture medium to directly produce algycone liquiritigenin. By applying this strategy, 67.5% of liquiritin was converted to liquiritigenin at pH 3.0 after 9 days of incubation and finally liquiritigenin was purified from the reaction mixture. And then, their biological activities including anti-oxidant and superoxide dismutase were observed. In fact, purified liquiritigenin was capable of bi-directional functions (i.e., either up-regulation or down-regulation of SIRT1 which is associated with aging). The results indicate that this strategy would be beneficial to produce biologically active liquiritigenin and could be used in pharmaceutical, cosmetic and food applications.