삽접법을 이용한 기계접목 메카니즘 연구

김주엽,이기명,박규식 慶北大學校農業科學技術硏究所 1997 慶北大農學誌 Vol.15 No.-

과채류의 재배에 있어서 접목은 작물의 안정적인 생산과 품질 향상을 위해 없어서는 안되는 기술이다. 그러나 지속적인 숙련 노동력의 감소와 농촌 인구 노령화 등으로 나타나는 노동력 부족 현상은 접목묘의 대량생산 공급에 어려움이 되고 있다. 본 연구에서는 기계접목에 있어서 접목법이 간단하며 접합부자재가 불필요한 삽접법을 이용하는 기계접목 메카니즘 개발의 기초연구를 수행하였다. 트레이상의 육묘상태에서 자동으로 접목하기 위한 기계접목의 기초연구로써 1본씩 수동 공급하는 반자동 기계삽접의 메카니즘 개발에 관한 연구로서 얻어진 결과를 요약하면 다음과 같다. 1. 접합부자재를 사용하지 않고 대부분 과채류에 적용가능한 삽접 기계접목장치의 메카니즘을 구성하였다. 2. 오이를 접수로 하고 신토좌와 흑종을 대목으로 하는 기계접목시험에서 모두 98%의 접목성공율을 나타냈다. 3. 1본 수동 공급에 의한 기계접목 시스템을 10초 정도로 접목 성능은 저조하지만 기계접목 메카니즘의 가능성을 확인하였다. Grafting is an important skill for the stable supply and production of high quality. However, the shortage of skillful labor has become great difficulty for a mass production of grafting-seedling. In this study, a suitable mechanism for a grafting machine was developed. The following summarize the results of this study: 1. An insertion method was selected for mechanism of the grafting machine without bonding agent, clip, pin. This insertion-grafting method can be applicable to general vegetables and a mass production system. In addition to, this method is suitable for developing the grafting mechanism. 2. Growing point was removed while remaining both cotyledons on rootstock. The productivity of this system was five fold greater than the one of an experienced labor. 3. The rootstock processing was placed on left and scion processing unit was placed on right of the system, then processed rootstock and scion graft by rotating 180˚. 4. The efficiency tests on mechanical grafting rate showed 98%.

Comparative analysis of the transcriptomes and primary metabolite profiles of adventitious roots of five Panax ginseng cultivars

Lee, Yun Sun,Park, Hyun-Seung,Lee, Dong-Kyu,Jayakodi, Murukarthick,Kim, Nam-Hoon,Lee, Sang-Choon,Kundu, Atreyee,Lee, Dong-Yup,Kim, Young Chang,In, Jun Gyo,Kwon, Sung Won,Yang, Tae-Jin The Korean Society of Ginseng 2017 Journal of Ginseng Research Vol.41 No.1

Background: Various Panax ginseng cultivars exhibit a range of diversity for morphological and physiological traits. However, there are few studies on diversity of metabolic profiles and genetic background to understand the complex metabolic pathway in ginseng. Methods: To understand the complex metabolic pathway and related genes in ginseng, we tried to conduct integrated analysis of primary metabolite profiles and related gene expression using five ginseng cultivars showing different morphology. We investigated primary metabolite profiles via gas chromatography-mass spectrometry (GC-MS) and analyzed transcriptomes by Illumina sequencing using adventitious roots grown under the same conditions to elucidate the differences in metabolism underlying such genetic diversity. Results: GC-MS analysis revealed that primary metabolite profiling allowed us to classify the five cultivars into three independent groups and the grouping was also explained by eight major primary metabolites as biomarkers. We selected three cultivars (Chunpoong, Cheongsun, and Sunhyang) to represent each group and analyzed their transcriptomes. We inspected 100 unigenes involved in seven primary metabolite biosynthesis pathways and found that 21 unigenes encoding 15 enzymes were differentially expressed among the three cultivars. Integrated analysis of transcriptomes and metabolomes revealed that the ginseng cultivars differ in primary metabolites as well as in the putative genes involved in the complex process of primary metabolic pathways. Conclusion: Our data derived from this integrated analysis provide insights into the underlying complexity of genes and metabolites that co-regulate flux through these pathways in ginseng.

Automatic Generation of Secondary Metabolite Biosynthetic Reactions to Update Host Strain"s Network

Kyu-Sang KYU-SANG,Hyun Uk KIM,Sang Yup LEE 한국생물공학회 2015 한국생물공학회 학술대회 Vol.2015 No.10

심인성 어지럼증 환자의 정신과적 증상에 관한 연구

이경규,이지영,김현우,이정엽,백기청,김재일,이근호 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.5

연구목적 : 어지럼증을 호소하는 환자들 중에 심인성 어지럼증 환자가 상당수 있다는 것은 알려져 있고, 이러한 심인성 어지럼증과 동반되는 정신질환으로는 불안장애, 우울장애, 인격장애 등이 있다. 이에 본 연구에서는 심인성 어지럼증 환자들의 빈도와 정신과적 증상을 알아보고자 하였다. 방 법 : 어지럼증 환자들에게 평형검사, 자세운동측정검사, 자세변화검사, caloric testing을 시행하여 심인성, 말초성 및 중추성의 3군으로 분류한 후 각각 14명, 16명, 32명을 대상으로 하였다. 대상환자들에게 한국판 Beck 우울척도검사(K-BDI), Spielberg의 상태-특성 불안검사(STAI). 간이정신진단검사(SCL-90-R)를 이용하여 정신과적 증상을 측정하여 이들 세 군을 비교분석하였다. 결 과 : 1) 심인성 어지럼증을 가진 환자는 어지럼증을 나타내는 환자 총 62명중에서 14명으로 22.6%를 차지하였다. 2) K-BDI에 따른 세 군간의 비교에서는 세 군간에 유의한 차이를 보이지 않았다. 3) STAI에 따른 세 군간의 비교에서 상태불안(STAI-S) 및 특성불안(STAI-T) 모두에서 세 군간에 유의한 차이를 보이지 않았다. 4) SCL-90-R에 따른 세 군간의 비교에서는 9개의 척도 중에서 공포불안척도에서만 중추성 어지럼증군이 47.56±6.90, 말초성 어지럼증 군이 53.50±13.74, 심인성 어지럼증 군이 58.50±16.05로 심인성 어지럼증 환자군이 중추성 어지럼증 환자군에 비하여 유의하게 높은 것으로 나타났다(p<0.01). 5) 통증 증상에 있어서는 세 군간에 유의한 차이를 보이지 않았고, 위장관 증상과 성기능 장애 증상은 중추성과 말초성 어지럼증 군이 심인성 어지럼증 군에 비하여 유의하게 높은 것으로 나타났다(p<0.01). 결 론 : 상기 결과를 토대로 했을 때 어지럼증 환자들에 대한 정신과적 접근을 위하여 각 군을 나누는 것은 별 의미가 없으며 어지럼증 환자들의 어지럼증 자체에 대한 직접적이고 집중적인 치료가 더욱 필요할 것으로 생각된다. Objectives : This study was aimed to investigate psychiatric symptoms in patients with psychogenic dizziness and compare these findings with those of patients with central and peripheral dizziness. Methods : A total of 62 patients with dizziness was the subject of investigation, and patients were classified into 32 with central type, 16 with peripheral type, and 14 with psychogenic type. Korean standardized Beck Depression Inventory(K-BDI), State and Trait Anxiety Inventory(STAI), and Korean standardized edition of Symptom Checklist 90 Revised(SCL-90-R) were used for the assessment. Statistically, Pearson's chi-square test and one-way ANOVA with Scheffe's test were used with SPSS/PC for windows 6.0. Results : The results were as follows : 1) The proportion of psychogenic dizziness was 22.6% of the total subjects. 2) Total scores of K-BDI were not significantly different among the 3 groups. 3) Total scores of state anxiety and trait anxiety were not significantly different among the 3 groups. 4) In SCL-90-R, psychogenic dizziness group showed significantly higher score of phobic anxiety only(p<0.01) than central dizziness group. And others were not significantly different among the 3 groups. 5) In additional somatic symptoms, pain score was not different among the 3 groups, but gastrointestinal and sexual symptoms scores of central and peripheral dizziness group were significantly higher than those of psychogenic dizziness group(p<0.01). Conclusion : These results suggest that psychiatric symptoms in patients with psychogenic dizziness are not different from those of patients with central or peripheral dizziness. Therefore, the more direct and intensive treatment may be necessary regardless of the type of dizziness.

Clinical and molecular characterizations of novel POU3F4 mutations reveal that DFN3 is due to null function of POU3F4 protein.

Lee, Hee Keun,Song, Mee Hyun,Kang, Myengmo,Lee, Jung Tae,Kong, Kyoung-Ah,Choi, Su-Jin,Lee, Kyu Yup,Venselaar, Hanka,Vriend, Gert,Lee, Won-Sang,Park, Hong-Joon,Kwon, Taeg Kyu,Bok, Jinwoong,Kim, Un-Kyun American Physiological Society 2009 PHYSIOLOGICAL GENOMICS Vol.39 No.3

<P>X-linked deafness type 3 (DFN3), the most prevalent X-linked form of hereditary deafness, is caused by mutations in the POU3F4 locus, which encodes a member of the POU family of transcription factors. Despite numerous reports on clinical evaluations and genetic analyses describing novel POU3F4 mutations, little is known about how such mutations affect normal functions of the POU3F4 protein and cause inner ear malformations and deafness. Here we describe three novel mutations of the POU3F4 gene and their clinical characterizations in three Korean families carrying deafness segregating at the DFN3 locus. The three mutations cause a substitution (p.Arg329Pro) or a deletion (p.Ser310del) of highly conserved amino acid residues in the POU homeodomain or a truncation that eliminates both DNA-binding domains (p.Ala116fs). In an attempt to better understand the molecular mechanisms underlying their inner ear defects, we examined the behavior of the normal and mutant forms of the POU3F4 protein in C3H/10T1/2 mesodermal cells. Protein modeling as well as in vitro assays demonstrated that these mutations are detrimental to the tertiary structure of the POU3F4 protein and severely affect its ability to bind DNA. All three mutated POU3F4 proteins failed to transactivate expression of a reporter gene. In addition, all three failed to inhibit the transcriptional activity of wild-type proteins when both wild-type and mutant proteins were coexpressed. Since most of the mutations reported for DFN3 thus far are associated with regions that encode the DNA binding domains of POU3F4, our results strongly suggest that the deafness in DFN3 patients is largely due to the null function of POU3F4.</P>

Old Age, Female Sex, and Psychotropic Medications Predict Hyposalivation But Not Heart Rate Variability

Seung-Yup Lee,Kyu-In Jung,Min-Hyeon Park,Chang Uk Lee 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.1

Objective To explore risk factors for dry mouth and examine the clinical utility of the heart rate variability (HRV) test in the prediction of dry mouth. Methods Every outpatient, who underwent tests for both unstimulated salivary flow and HRV, was retrospectively reviewed. After excluding seven subjects, the demographics and clinical factors in 70 total patients were collected. Based on objective salivary flow rates, patients were classified into normal (≥0.2 mL/min) or hyposalivation groups (<0.2 mL/min), and inter-group comparisons were performed with a two-tailed statistical significance of 0.05. Results Patients with subjective dry mouth were significantly more likely to show hyposalivation. Advanced age, female sex, and current use of psychotropic medications were identified as risk factors for dry mouth. However, dry mouth was not associated with any HRV parameters. Conclusion HRV test did not demonstrate a clinical utility in predicting dry mouth. Because subjective dry mouth is significantly associated with objective hyposalivation, a simple probing question would be useful for early recognition of dry mouth. Clinical attention is required for patients meeting criteria of older age, female, and/or using psychotropic prescriptions. Prompt management of hyposalivation may improve quality of life and clinical outcome by enhanced treatment adherence.

알츠하이머병에서 우울 증상과 고혈압의 관계

김규동(Kyu-Dong Kim),이승엽(Seung-Yup Lee),이해국(Hae-Kook Lee),이경욱(Kyoung-Uk Lee),권용실( Yong-Sil Kweon),이정태(Chung-Tai Lee) 대한노인정신의학회 2017 노인정신의학 Vol.21 No.2

Objective:To explore the effect of vascular risk factors to the depressive symptom in Alzheimer’s disease (AD). Methods:Retrospective chart reviews were performed for the patients, who underwent comprehensive neuropsychiatric assessment in the geriatric psychiatric clinic in the period between May of 2015 and January of 2017. Patients with moderate to severe stage of AD, neurological comorbidities and major psychiatric disorders were excluded to minimize the confounding factors. Sixty five newly diagnosed early AD patients were included and bisected by the cutoff point of 5 in the short version of the Geriatric Depression Scale. Comparisons were done between two groups for vascular risk factors and other relevant clinic-demographic variables. Binary logistic regression analysis was additionally performed to estimate the effect of hypertension to the depressive symptoms. Results:Among the investigated vascular risk factors, hypertension was associated with depressive symptom in the early AD patients. In the logistic regression, odd ratio of hypertension for depression was 4.34 (95% confidence interval 1.47-12.79). Conclusion:Depressive symptoms in the AD patients were associated with hypertension. Therefore, managing this vascular factor in the middle age may not only decrease the risk for neurovascular disorders, but also provide additional benefits in curtailing depression in AD.

Toll-like receptor 2 signaling protects against ultraviolet-induced inflammation

( Dong Hun Lee ),( Youngae Lee ),( Chang Yup Shin ),( Serah Lee ),( Kyoung Hwan Kong ),( Ji Eun Kim ),( Kyu Han Kim ),( Jin Ho Chung ) 대한피부과학회 2012 대한피부과학회 학술발표대회집 Vol.64 No.3

Integrated analysis of the transcriptomes and primary metabolite profiles of adventitious roots of P. ginseng cultivars

Yun Sun Lee,Hyun-Seung Park,Dong-Kyu Lee,Murukarthick Jayakodi,Nam-Hoon Kim,Sang-Choon Lee,Jinkyung Kim,Hana Lee,Dong-Yup Lee,Sung Won Kwon,Tae-Jin Yang 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Panax ginseng C.A. meyer (family: Araliaceae) is a perennial crop that has been widely used as a traditional medicine in Korea. Various P. ginseng cultivars exhibit a range of morphological and physiological traits as well as genetic diversity. To elucidate the differences of primary metabolism underlying such genetic diverstiy, we performed primary metabolite profiles in adventitious roots from five Panax ginseng cultivars using gas chromatography-mass spectrometry (GC-MS). The GC-MS analysis revealed eight primary metabolites as biomarkers and allowed us to classify the five cultivars into three groups. We selected three cultivars to represent each group and analyzed their transcriptomes by Illumina sequencing. We inspected 100 unigenes involved in seven primary metabolite biosynthesis pathways and found that 21 unigenes encoding 15 enzymes were differentially expressed among the three cultivars. Integrated analysis of transcriptomes and metabolomes revealed that the ginseng cultivars differ in primary metabolites as well as in the putative genes involved in the complex process of primary metabolic pathways. Our data derived from this integrated analysis provide insights into the underlying complexity of genes and metabolites that co-regulate flux through these pathways in ginseng.

[약학] 디-2-피리딜케톤을 이용한 산성의약품 선택성 막전극의 특성

이동엽(Dong-Yup Lee),이재윤(Jae-Yoon Lee),안문규(Moon-Kyu Ahn) 한국분석과학회 2003 분석과학 Vol.16 No.4