NOD/SCID 마우스 모델을 이용한 인간 제대혈 혼합이식에서의 생착 양상 분석

정양조,김동욱,조빈,강영주,박보배,김혜정,김태규,오일환 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.2

연구배경: 제대혈은 골수에 비해 많은 장점을 가지고 있음에도, 이식 후 성적에 있어 총세포수에 의해 좌우되며, 한 개의 태반으로부터 얻을 수 있는 총세포수의 한계가 있어 주로 소아에 국한되고 있다. 따라서 제대혈 응용의 확대를 위해 생착량을 늘이기 위한 방법으로 다중공여자에 의한 제대혈 혼합이식을 늘일 수 있는지 연구하기 위하여, 본 연구에서는 주조직적합성 형별로 조합된 제대혈을 NOD/ SCID 생쥐에 이식하는 방법으로, 혼합생착의 가능성을 연구하였다. 방법: 조합된 2개 공여자로부터 얻어진 제대혈들을 단일이식 및 혼합이식하여, 혼합이식된 숙주에 생착된 세포를 PCR-SSOP 방법을 사용하여 공여자 별로 추적하였다. 또한 각 공여자세포의 상대적 생착비율을 대조군인 단일제대혈 이식에서 얻어진 생착량과 비교하는 정량적 비교도 함께 하였다. 결과: 총단핵구를 혼합이식한 실험군에서는 주조직적합성의 일치도에 관계없이 한쪽공여자의 세포가 다른쪽에 비해 우세한 편향생착을 보였다. 그러나 임파구를 비롯한 Lineage 양성세포를 제거한 실험군에서는 주조직적합성의 불일치에도 불구하고 뚜렸한 생착의 공존이 관찰되었다. 결론: 본 연구는 제대혈의 생착량을 증가를 목적으로 총단핵구를 혼합이식할 경우 한쪽공여자의 세포에 의해 편중되며, 이것을 이식 후 생착과정에서의 두 종류의 세포의 경쟁에 의해 초래된다는 것을 보이고 있다. 또한 이러한 경쟁은 제대혈에 포함되어 있던 임파구들에 의한 것이며, 생착공존을 위하여는 공여자간 주조직적합성의 일치도 보다, 임파구의 제거가 더 중요하다는 것을 보여주고 있다. Background: Although umbilical cord blood (UCB) has been an attractive alternatives for bone marrow cells, application is limited to pediatric case due to limitation in total number of nucleated cells, that is a single most important factor for outcomes of UCB transplantation. Therefore, increasing overall engraftment by mixed transplantation of UCB derived from multiple donor should comprise one strategy to circumvent the posed limitation of UCB transplantation. In order to investigate the feasibility of establishing co-engraftment by multi-donor UCB cells, we carried out a xenotransaplantation study using NOD/SCID mice for systemic analysis of results on the input-based control of single unit transplantation. Methods: UCB units with various extent of HLA-matchings were co-transplanted into NOD/SCID mice along with single unit transplantation control. The relative contributions of engraftment by cells from each donor-derived were analyzed by HLA polymorphism using PCR-SSOP. Results: In all HLA-based mixed transplantation of total nucleated UCB cells, engraftment of one donor predominated over the other despite that equivalent amount of engraftments were achieved by single donor transplantation. When lineage depleted UCB units were co-transplanted, significant degree of co-engraftments were observed regardless of HLA disparity. Conclusion: Our result show that one donor cells dominate over the other in mixed UCB transplantation and that it is due to competition between donor cells during post-transplantation process. Our results suggest that immune cells contained in UCB unit mediate such competitioin and that 6 locus HLA matching would not be sufficient to prevent the competition.

FLASH Radiotherapy: A FLASHing Idea to Preserve Neurocognitive Function

( Hye-ju Jo ),( Taerim Oh ),( Ye-rim Lee ),( Gi-sue Kang ),( Hye-joon Park ),( G-one Ahn ) 대한뇌종양학회·대한신경종양학회·대한소아뇌종양학회 2023 Brain Tumor Research and Treatment Vol.11 No.4

FLASH radiotherapy (FLASH RT) is a technique to deliver ultra-high dose rate in a fraction of a second. Evidence from experimental animal models suggest that FLASH RT spares various normal tissues including the lung, gastrointestinal track, and brain from radiation-induced toxicity (a phenomenon known as FLASH effect), which is otherwise commonly observed with conventional dose rate RT. However, it is not simply the ultra-high dose rate alone that brings the FLASH effect. Multiple parameters such as instantaneous dose rate, pulse size, pulse repetition frequency, and the total duration of exposure all need to be carefully optimized simultaneously. Furthermore it is critical to validate FLASH effects in an in vivo experimental model system. The exact molecular mechanism responsible for this FLASH effect is not yet understood although a number of hypotheses have been proposed including oxygen depletion and less reactive oxygen species (ROS) production by FLASH RT, and enhanced ability of normal tissues to handle ROS and labile iron pool compared to tumors. In this review, we briefly overview the process of ionization event and history of radiotherapy and fractionation of ionizing radiation. We also highlight some of the latest FLASH RT reviews and results with a special interest to neurocognitive protection in rodent model with whole brain irradiation. Lastly we discuss some of the issues remain to be answered with FLASH RT including undefined molecular mechanism, lack of standardized parameters, low penetration depth for electron beam, and tumor hypoxia still being a major hurdle for local control. Nevertheless, researchers are close to having all answers to the issues that we have raised, hence we believe that advancement of FLASH RT will be made more quickly than one can anticipate.

합성곱 신경망 기반 미세한 책 소리 분류 및 활용

김혜주(Hye-Ju Kim),김승찬(Seung-Chan Kim) 한국HCI학회 2020 한국HCI학회 학술대회 Vol.2020 No.2

소비자들은 도서 구매를 할 때 여러 책을 훑어보면서 관심이 가는 책은 좀 더 꼼꼼히 살펴보는 행동을 하게 된다. 책에 대한 소비자의 관심 정도를 파악하는 방법은 대부분 설문조사를 통해서 이루어진다. 만약 주관적인 설문조사 방식이 아닌 소비자가 책을 어떠한 방식으로 읽고 있는지 정량적으로 파악할 수 있다면 어떤 책이 관심을 끌었는지 쉽게 파악할 수 있을 것이다. 이러한 맥락에서 본 논문에서는 책을 다룰 때 발생하는 미세한 소리에 근거하여 사용자가 책을 다루는 방법을 정량화하고자 한다. 본 연구에서는 예비 연구 (preliminary work)로서 합성곱 신경망 (Convolutional Neural Network)을 이용하여 사용자의 책을 읽는 동작을 미세한 소리에 근거하여 분류 및 예측하는 방법을 제안한다. 타당성 검토를 위해 본 연구에서는 발생된 소리의 시간-주파수 특성을 분석하고자 스펙트로그램으로 표현하고, 이를 합성곱 신경망을 이용하여 학습해 사전 정의된 동작을 예측해냈다. 실험을 통해 책을 통한 8 가지 동작에 대한 분석을 시도하여 소리만으로 사용자의 동작을 정교하게 예측해 냄을 확인하였다.

석유화학플랜트 가상현실 안전훈련 및 매뉴얼 개발

윤혜주(Hye-Ju Yun),유철희(Chul-Hee Yu),조영훈(Young-Hoon Jo),김영진(Young-Jin Kim),조영도(Young-Do Jo) 한국가스학회 2018 한국가스학회 학술대회논문집 Vol.2018 No.11

Repeated Water Avoidance Stress Alters Mucosal Mast Cell Counts, Interleukin-1β Levels with Sex Differences in the Distal Colon of Wistar Rats

( Ju Yup Lee ),( Nayoung Kim ),( Yong Sung Kim ),( Ryoung Hee Nam ),( Min Hee Ham ),( Hye Seung Lee ),( Wonjun Jo ),( Youngkwang Shim ),( Yoon Jin Choi ),( Hyuk Yoon ),( Cheol Min Shin ),( Dong Ho Lee 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2016 Journal of Neurogastroenterology and Motility (JNM Vol.22 No.4

Background/Aims This study was aimed at evaluating differences in the effects of repeated water avoidance stress (rWAS) on colonic movement, mucosal mast cell counts, cytokine levels, and visceromotor response (VMR) to colorectal distension (CRD) in rats of both sexes. Methods Wistar rats were divided into stress and no-stress groups. Rats in the stress group were exposed to rWAS (1 hr/day) for 10 days. Mucosal mast cells were immunohistochemically stained with anti-mast cell tryptase antibody and counted. The colonic mucosal cytokine levels were measured with enzyme-linked immunosorbent assay. The VMR to CRD (visceral analgesia) was assessed by using a barostat and noninvasive manometry. Results The mean number of fecal pellets in the rWAS group increased significantly as compared with that in the no-stress group in both sexes. After adjustment for body weight, the female rats had a significantly higher pellet output than the male rats. The mucosal mast cell count of the female rWAS group was higher than that of the male rWAS group (13.0 ± 0.9 vs 8.8 ± 0.6; P < 0.001). The colonic mucosal interleukin-1β level was also higher only in the female rats of the rWAS group than in those of the no-stress group. On days 10 and 11, a decrease in VMR to CRD was observed at 40 and 60 mmHg in both sexes of the rWAS group, without a sex-based difference. Conclusions The colonic response to stress appeared to be more sensitive in the female rats than in the male rats. However, stress-induced visceral analgesia had no sex-related difference and the underlying mechanism needs to be further evaluated.

The Characteristics of Fine-Sized LiMn_(12-X)/6Al_x/6O₄(0.05≤x≤2) Powders Prepared by Spray Pyrolysis

Ju, Seo Hee,Kim, Do Youp,Koo, Hye Young,Hong, Seung Kwon,Jo, Eun Byul,Kang, Yun Chan Trans Tech Publications, Ltd. 2007 Key Engineering Materials Vol.350 No.-

<P>The series of partially Al-substituted lithium manganese oxides were prepared by spray pyrolysis process from spray solutions with citric acid and ethylene glycol. The as-prepared particles obtained from spray solution with citric acid and ethylene glycol turned to fine-sized LiMn(12- x)/6Alx/6O4(0.05≤x≤2) particles with regular morphology after post-annealing. The discharge capacities of the particles post-annealed at temperature of 800oC changed from 128 to 84 mAh/g when the x of the LiMn(12-x)/6Alx/6O4 particles was changed from 0 to 2. The Al-doped LiMn2O4 fine particles prepared by spray pyrolysis from spray solution with citric acid and ethylene glycol had good cycle properties.</P>

S-343 : The Effects of Administration of Exendin-4 in Obese Type 2 Diabetes Mellitus

( Ju Young Hong ),( Dong Mi Lim ),( Keun Young Park ),( Hee Kwan Won ),( Byung Joon Kim ),( Hye Jin Youn ),( Areum Jo ) 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1

The majority of patients with type 2 DM (diabetes mellitus) are obese and suffer from eating disorders; Recently developed Exendin-4 is known to reduce appetite and to have a weight loss benefit without experiencing hypoglycemia which can be caused by physiological insulin secretion. Therefore, the authors aim to examine the effects of Exendin-4 on the body composition of obese type 2 DM patients. 5 ug of Exendin-4 was initially administered to type 2 DM patients whose BMI (body mass index) was above 25 kg/cm2 twice a day 1 hour prior to the meal for a month and the dosage was increased to 10 ug afterwards. The patients` weight, BMI, body fat percentage, body fat mass and muscle mass, HbA1c, Lipid were measured with an inbody prior to the experiment, after the 3 months The average weight and the average BMI of the total 21 subjects in this study were 90.82±12.08 kg and 33.67±4.00 kg/cm2 respectively. The initial body fat percentage, the muscle mass and the body fat mass were 40.27±7.12, 30.05±5.90, 36.56±7.96 respectively. The weight, the BMI, body fat percentage, the muscle mass and the body fat mass measured after 3 month were 87.47±14.22 (p=0.002), 32.35±4.21 (p=002), 38.33±7.24 (p=0.014), 29.79±5.90 (p=0.376) and 33.70±8.70 (0.002) respectively. The initial HbA1c, Triglyceride, Total cholesterol, Low density lipoprotein, AST, ALT were 8.80±1.74, 215.54±130.52, 178.76±51.08, 109.62±37.16, 34.39±21.69, 37.09±23.12. The differences after three months were recorded as 7.90±1.50 (p=0.008), 174.33±74.69 (p=0.142), 154.42±36.13 (p=0.03), 98.48±29.06 (0.199), 31.52±18.24 (p=0.36), 37.09±23.12 (p=0.97) The short-term use of Exedin-4 in bese type 2 DM patients reduced BMI, body fat percentage as well as HbA1c and Total cholesterl without losing muscle mass and impairing liver function.

1987년 가을철에 경기도 여주지역에 발생한 렙토스피라병 23례

조기주,서학은,이혜순,박성림,황의정,홍명호,김민자 대한가정의학회 1988 Korean Journal of Family Medicine Vol.9 No.12

Process viscometry in flows of non-Newtonian fluids using an anchor agitator

Jo, Hae Jin,Jang, Hye Kyeong,Kim, Young Ju,Hwang, Wook Ryol Springer Science + Business Media 2017 Korea-Australia rheology journal Vol.29 No.4

Self Assembled Block Copolymer Gate Insulators with Cylindrical Nanostructures for Pentacene Thin Film Transistor

Jo, Pil-Sung,Park, Youn-Jung,Kang, Seok-Ju,Kim, Tae-Hee,Park, Cheol-Min,Kim, Eun-Hye,Ryu, Du-Yeol,Kim, Ho-Cheol 한국고분자학회 2010 Macromolecular Research Vol.18 No.8

This study examined the effect of a chemically nanostructured surface of cylinder forming poly(styrene-b-methyl methacrylate) (PS-b-PMMA) and poly(styrene-b-4vinyl pyridine) (PS-b-P4VP) block copolymer gate dielectrics on the performance of the bottom gate pentacene organic thin film transistor (OTFT). The field effect mobility of pentacene is affected mainly by the chemical properties of the top skin of a block copolymer layer. In the case of PS-b-PMMA with cylindrical PMMA microdomains that are located very closely at the block copolymer-pentacene interface because the surface energy of PMMA is similar to that of PS, the field effect mobility in general corresponds to the area averaged value of the two mobilities with the pure PS and PMMA layer. On the other hand, PS-b-P4VP copolymer results in a similar field effect mobility to that of the pure PS layer because the cylindrical P4VP microdomains are embedded in the PS matrix of which the surface energy is much lower than that of P4VP. The orientation of the cylindrical PMMA microdomains with respect to the surface also affects the field effect mobility, where the PMMA microdomains are aligned perpendicular to the surface, gave rise to a mobility approximately 50% higher than those parallel to the surface. The composite model with parallel and series resistance units offers qualitative understanding of these results.