Dimethylnitrosamine 유발 급성 간 손상 흰쥐에서 ^(99m)Tc-Lactosylated Serum Albumin을 이용한 간 기능의 평가

정신영,이재태,서명랑,유정아,배진호,안병철,황재석,정재민,하정희,이규보 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.6

목적: ^(99m)Tc-lactosylated serum albumin (^(99m)Tc-LSA)은 간세포에 특이적으로 결합하는 간수용체 영상용 방사성의약품으로 새로이 합성되었다. 간섬유화를 유발하는 dimethylnitrosamine (DMN)을 투여한 간 손상 휜쥐 모델에서 ^(99m)Tc-LSa의 역동학적인 간섭취를 조사하고 간효소치의 변화와 조직학적 소견을 비교하여, LSA의 간섭취가 간기능의 변화를 반영하는지를 연구하였다. 대상 및 방법: SD계 흰쥐에 DMN를 27 mg/kg으로 복강 내 주사하여 급성 간손상을 유도하고 대조군과 비교하였다. DMN을 주사한 흰쥐를 3일(DMN-3), 8일(DMN-8), 21일(DMN-21)에 ^(99m)Tc=LSA (1,665 mg/kg) 29 MBq를 정맥 주사하여, 30분 동안 동적 영상을 획득하고 간과 신장부위에 관심영역을 설정하여 간과 심장부위의 시간방사능 곡선을 얻었다. 간기능 평가를 위해 시간방사능 곡선을 이용하여 간섭취지수와 혈중제거지수를 구하였고 곡선 최적화를 시행하였다. DMN 투여군과 대조군의 간효소치의 변화와 간조직의 광학현미경 소견을 비교하였다. 결과: 대조군에서는 ^(99m)Tc-LSA가 빠르게 간에 섭취되고 혈중에서 제거되었으나 DMN을 처리한 군에서는 간섭취가 낮았다. 간섭취지수의 비교에서 대조군에 비해 DMN 처리군에서 유의하게 간섭취지수가 낮았다(DMN-3: 0.842, DMN-8: 0.898, DMN-21: 0.91, 대조군: 0.96, p<0.05). 혈중제거지수의 비교에서도 대조군에 비해 DMN 처리군에서 혈중제거지수가 유의하게 높았다(DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, 대조군: 0.473, p<0.05). 비선형 회귀분석에서 R_(2) 값은 0.9이상으로 좋은 일치를 보였고, 대조군에ㅓ K값이 DMN처리군에 비해 크고(DMN-3: 0.28, DMN-8: 0.41, DMN-21: 0.46, 대조군: 0.97, p<0.05), T_(1/2)값은 작았다(DMN-3: 2.5, DMN-8: 1.7, DMN-21: 1.5, 대조군: 0.7, p<0.05). 간효소치의 변화는 DMN-3군에서는 대조군에 비해 상승하였으나 DMN-8, DMN-21군에서는 간효소치의 상승이 관찰되지 않았다. 간조직 소견의 경우 DMN-3군에서 중심정맥 주위에 괴사가 관찰되었으나 DMN-8군, DMN-21군에서는 미약한 정도의 염증세포 침윤만이 관찰되었다. 결론: ^(99m)Tc-LSA 간신티그래피의 간섭취 정도는 간손상과 반비례하였으며 간섭취의 변화는 조직학적 손상이 심한 정도와 간손상후 회복되는 과정을 반영하여 주었다. ^(99m)Tc-LSA 간신티그래피가 간손상을 평가하고 간손상후 회복되는 과정을 추적하는 간수용체 영상용 방사성 의약품으로 사용될 수 있을 것으로 생각된다. Objects: ^(99m)Tc-lactosylated human serum albumin(LSA) is a newly synthesized radiopharmaceutical that binds to asialoglycoprotein receptors, which are specifically presented on the hepatocyte membrane. Hepatic uptake and blood clearance of LSA were evaluated in rat with acute hepatic injury induced by dimethylnitrosamine(DMN) and results were compared with corresponding findings of liver enzyme profile and these of histologic changes. Materials and Methods: DMN (27 mg/kg) was injected intraperitoneally in Sprague-Dawley rat to induce acute hepatic injury. At 3(DMN-3), 8(DMN-8), and 21(DMN-21) days after injection of DMN, LSA injected intravenously, and dynamic images of the liver and heart were recorded for 30 minutes. Time-activity curves of the heart and liver were generated from regions of interest drawn over liver and heart area. Degree of hepatic uptake and blood clearance of LSA were evaluated with visual interpretation and semiquantitative analysis using parameters (receptor index : LHL3 and index of blood clearance : HH3), analysis of time-activity curve was also performed with curve fitting using Prism program. Results: Visual assessment of LSA images revealed decreased hepatic uptake in DMN treated rat, compared to control group. In semiquantitative analysis, LHL3 was significantly lower in DMN treated rat group than control rat group (DMN-3:0.842, DMN-8: 0.898, DMN-21: 0.91, Control: 0.96, p<0.05), whereas HH3 was significantly higher than control rat group (DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, Control: 0.473, p<0.05). AST and ALT were significantly higher in DMN-3 group than those of control group. Centrilobular necrosis and infiltration of inflammatory cells were most prominent in DMN-3 group, and were decreased over time. Conclusion: The degree of hepatic uptake of LSA was inversely correlated with liver transaminase and degree of histologic liver injury in rat with acute hepatic injury.

도시 주부의 생활환경 문제에 대한 의식과 관리행동

황경혜,정진희,이정우 숙명여자대학교 생활과학연구소 1993 生活科學硏究誌 Vol.8 No.-

The purpose of this study is to investigate the urban housewives and the level of their consciousness and management in the problems of living environment, and to analize the major factors which make various effects. On the findings of these data, author myself is to inspire the environmental accountability and the role in environment preservation, and to provide the grounding materials, by which housewives are realistically awakened to their new significance in the environment problems in the life area. The period of investigation was a month in august, 1993, and the subject of it was limited to the housewives who were living in Kwang-Ju. the 650 sheets of questionnaires were distributed to them and the 536 sheets of them were used in research materials. The data which was obtained got the frequency percentage and the arithmetic mean by way of SAS, and were analyzed by F-test, Duncan's multiful rangement, Pearson's coefficient analysis and stepwise multiful regression. The major findings is summarized as follows: 1) The average score of housewives consciousness and management behavior on the problems of living environment are 74.14 on the scale of 115 (64.46 on the scale of 100) and 69.80 on the scale of 115 (60.69 on the scale of 100). 2) Housewives'age, education and income, the attitude on the household labor, the satisfaction in the family life and the perceptibility in the time pressure are the variables which have an influence on their consciousness in the problems of the living environment, while their age, education, the satisfaction of the family life and the attitude on the household labor are the variables which have an influence on their management behavior in them. 3) There are relatively a positive correlationship between their consciousness and management behavior in the problems of the living environment. 4) It appears that their management behavior on the problems in the living environment has a major correlationship with the consciousness on them, the education, the income and the attitude on the household labor. Based on the above result, the change of consciousness are required in the problems of the living environment. Accordingly, we should develop enough programs and materials in the environment education, and take the lifelong education and the data development.

골격근 근형질세망의 ATP유도 ^45Ca-uptake에 대한 Thapsigargin및 Vanillylnonanamide의 영향

황의강,배소현,홍장희,허강민,김진회,이재흔,석정호 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.2

To investigate the effect of thapsigargin(THP) and vanillylnonanamide(VN), derivative of capsaicin, on the ATP-induced ^45Ca-uptake in the sarcoplasmic reticulum(SR) of the skeletal muscle, the SR vesicles were prepared from the back muscle of the rabbit, and ^45Ca-uptake was carried out. The results as follows: ATP-induced ^45Ca-uptake of skeletal muscular SR was significantly increased by 1 mM ATP. It was significantly blocked by 5 μM THP, but slightly decreased by 20 μM VN. The combined effect of THP and VN on the 45Ca-uptake of the SR vesicles was much potentiated than the sum of each effect of them. The above results suggest that the action of VN, being slightly influential to ATP-induced Ca-uptake but potentiating the effect of THP on the Ca-uptake, might be related with direct perturbation of the SR membrane or exposure of the THP-binding site.

골격성 Ⅲ급 부정교합 환자에서 술전 교정전 예측치와 교정후 실측치의 차이에 관한 연구

황충주,권희정 대한치과교정학회 2003 대한치과교정학회지 Vol.33 No.1

본 연구에서는, 술전 교정전 예측전와 교정후 실측치 사이에 나타나는 오차의 크기와 정확성 및 어떠한 요소가 그러한 결과에 영향을 미치는가에 대해 평가하고자 하였다. 연세대학교 치과병원 교정과에서 골격성 Ⅲ급 부정교합ㅇ로 진단되어 술전 교정치료 및 양악수술을 받은 환자 가운데 조건에 부합되는 45명(남자 17명, 여자 28명)을 선별하여, 초진 상태를 T1, 교정 치료전 예측치를 T2, 술전 교정후를 T3로 정의하고 각각의 계측치를 수평과 수직좌표로 나누어 측정하였다. 또한 환자의 어떠한 진단적 요소가 술전교정의 예측치와 실측치 사이의 차이에 영향을 미치는지 알아보기 위해 연구대상을 arch length discrepancy (ALD), 상·하악 전치의 치축(U1 to SN, IMPA), curve of Epee(COS), 발치 여부, 발치한 치아의 종류 등에 의해 여러 군으로 분류하여 유의성을 평가하였으며 다음과 강은 결과를 얻었다. 1. U6mbc, L1x의 수평 좌표와 Uli, Ulx, U6me, U6mbc, L6mbc의 수직 좌표에서 예측한 위치와 수술직전의 실제 위치의 차이가 유의성(p<0.05) 있게 나타났다. 2. 예측의 정확성은 수평적인 차이의 예측에 있어서 수직적인 차이의 예측에 비해 상대적으로 높게 나타났다. 3. 하악에서보다 상악의 많은 계측점에서 예측치와 실측치 사이의 유의한 차이(p<0.05)가 관찰되었다. 4. 상악의 경우 발치 여부 및 발치한 치아의 종류, ALD의 양에 따라 상악 전치와 제1대구 위치의 예측에 유의한 차이(p<0.05)를 보였으며, 하악의 경우에는 ALD의 양과 IMPA에 따라 하악 전치와 제1대구의 위치의 예측에 유의한 차이(p<0.05)를 나타냈다. 이번 연구에서 얻은 결과를 통하여 술전 교정전 예측치와 실측치 사이의 오차의 정도와 그것에 영향을 미치는 요소들을 분석하고 희귀 계수를 구함으로써, 악교정 수술을 동반한 교정 환자의 치료 계획 시 예측의 정확성을 높일 수 있을 것으로 생각된다. 또한 수직적인 예측에 있어서는 앞으로 더 많은 연구가 이루어져야 할 것으로 생각된다. The Purpose of this study was to find out and evaluate discrepancies between preoorthodontic prediction values and actual postorthodontic values and factors contributing to it 45 patients(17 male, 28 female) who were diagnosed as skeletal Class Ⅲ malocclusion and received presurgical orthodontic treatment and orthognathic surgery at Yonsei university dental hospital. Lateral cephalograms were analyzed at oretreatment(T1), orthodontic prediction(T2), immediately before surgery(T3) and designated the landmark as coordinates of X and Y axes. The samples were divided according to ALD, upper and lower incisor inclination(U1 to SN, IMPA), COS, extraction, the position of extracted teeth and the statistical significance was tested to find out the factors contributing to the prediction. The results were as follows : 1. Differences between preorthodontic prediction values and actual postorthodontic values(T2-T3) were statistically significant(p<0.05) in the x coordinates of U6mbc, L1x and in y coordinates of U1i, Ulx, U6me, U6mbc, L6mbc 2. The accuracy of prediction is relatively higher in horizontal changes compared to vertical changes. 3. The statistical significance(p<0.05) between prediction and actual values is observed more in the landmarks of the maxilla than the mandible. 4. differences between prediction and actual values of incisor and first molar were statistically significant(p<0.05) according to extraction vs non-extraction, extraction type, ALD in the maxilla and according to ALD< IMPA in the mandible. Discrepancies between preorthodontic prediction values and actual postorthodontic values and factors contributing to the prediction must be considered in treatment planning of Cl Ⅲ surgical patients to increase the accuracy of prediction. Furthermore future investigations are needed on the prediction of vertical changes.

평양 남정리 53호분 출토 토제칠기의 보존 및 제작기법 연구 : 토제칠이배, 토제칠반, 토제칠안

황현성,임수경,김희진,이태진,고민정,윤은영 국립중앙박물관 2013 고고학지 Vol.19 No.-

본고는 일제 강점기 조사 미등록 유물 정리사업의 일환으로 수행한 평양 남정리 53호분 출토 토제칠이배, 토제칠반, 토제칠안에 관한 연구이다. 남정리 출토 유물은 소지가 목심이 아닌 토제라는 것과 칠이배와 칠반을 얹은 칠안이 함께 출토되었다는 점에서 주목할 만하다. 토제칠이배 및 칠반 그리고 칠안의 보존처리를 통해 이들의 형태와 문양 등에 대해 알 수 있었다. 또한 칠기에 사용된 붉은색 안료의 성분을 분석하여 안료의 주성분이 진사(HgS)였음을 확인하였다. 이번 연구에서 기존에 잘 알려지지 않았던 한반도 내 위치한 낙랑의 토제칠기에 관해 살펴볼 수 있었으며 이러한 제작방법이 한나라 고유의 문화인지 아니면 재지세력이 한나라의 영향을 받아 현지화한 문화의 산물인지에 대해 생각해 볼 수 있는 기회라 생각된다. This research paper examines the lacquered pottery cup, plate and table unearthed from Tomb No. 53 at Namjeong-ri in Pyongyang, which were under conservation treatment as a part of the Project of Unregistered Artefacts Uncovered in the Japanese Colonial Era. It is very rare to have a lacquered pottery cup and plate, lying on a lacquered pottery table, as a set of grave goods. In particular, they are not wooden but pottery items. Through conservation treatment for lacquered pottery cup, plate and table, it was possible to find out their shapes and decoration patterns. The resulting analysis of the red pigment used indicates that cinnabar (HgS) is the main element. This research is significant because it examines lacquered pottery wares produced in Lelang, which has rarely been conducted. Further research will need to prove whether their production techniques were in the unique Chinese Han style or were produced by indigenous craftsmen who adopted the Chinese Han technique.

금피복된 임플란트 보철용 유지나사의 예비하중 및 풀림회전력에 관한 연구

정창모,황희성,전영찬,최설희 大韓齒科器材學會 2000 대한치과재료학회지 Vol.27 No.3

The purpose of this study was to compare the preload and the removal torque produced when using gold coated prosthetic retaining screws (Gold-Tite retaining screws) and non-coated screws(gold screws) repeatedly and to evaluate effects of a thin pure gold coating on screw stability. Following connection of the conical abutment to implant fixture using 20 Ncm of torque applied by a torque gauge, fastened the strain-gauged gold cylinder to conical abutment with gold screw, Gold-Tite retaining screw using 10Ncm. Then, preloads and removal torques were measured, screws tightened and loosened 8 times repeatedly. All experiments were performed under dry and wet condition. The results obtained were summarized as follows : 1. For gold screw group, no significant differences existed in the preload and the removal torque according to trials in both conditions(p>0.05). 2. For Gold-Tite retaining screw group, no significant differences existed in the preload according to trials in both conditions(p>0.05). However, the removal torque decreased at the second trial and incresed, after then(p<0.05). 3. In comparison of preloads according to trials, the preload in dry condition was greater than in wet condition only for Gold-Tite retaining screw group after the fourth trial(p<0.05). However, no significant differences were found between gold screw group and Gold-Tite retaining screw group(p>0.05). 4. In comparison of removal torque according to trials, the removal torque of gold screw group was greater than of Gold-Tite retaining screw group in dry condition at the second, third trial(p<0.05). At the other trials, no significant differences were found among the experimental groups(p>0.05).

영덕 달산 지역 화산층서와 용암의 공급지

황상구,함희수,김지영,정해오 안동대학교 기초과학연구소 1999 基礎科學 硏究論文集 Vol.10 No.1

We traced the flow direction of Ipbong Andesite and dacite in Dalsan area, Yeongdeok. Elongate vesicles and lathlike plagioclase phenocysts are aligned in the outcrops or under microscope. The lineations from their alignment are excellently indicating the flow direction of lavas. The flow directions, which were measured from the lineations in the Ipbong Andesite, are NNW-SSE in the eastern part and NNE-SSW in the southwestern part. The flow lineations show a fanlike movement pattern from the mid-northern part that the diorite is distributed. The diorite area is prabably a potential source area of the andesite

農民所得增大를 위한 고구마의 栽培와 利用에 관한 硏究

黃鍾奎,梁熙天,崔定植,權涌周 全北大學校 1977 論文集 Vol.19 No.-

New varieties of sweet potato Sinmi and Hwangmi were cultivated in the reclaimed hilly area of four kinds of soil series in Jeonbug province and their growth conditions such as cutting time, row spacing, application of fertilizers and plant growth regulators and covering effect were investigated. Besides these studies on cultivation of sweet potatoes, experiments on the change of chemical components of sweet potato tubers, adaptibility for storage and drying of sliced sweet potato tubers were carried out. The results are summarized as follows : 1. The early cutting of May 20, alley of 90 X 20cm, application of potassium(28kg/10a)and covering produced the high yield of sweet potatoes. The apllication of OED green did not increase the yield of sweet potatoes, 2. The yield of Hwangmi was higher than that of Sinmi. These two varieties of sweet potatoes grew well in all the test soil series except Bansan. By early cutting of May 20, the highest yield of Hwangmi was obtained in Songjung series soil and the highest yield of Sinmi was in Yesan. 3. Starch content was higher in Sinmi than in Hwangmi while yield of starch per unit area was high in Hwangmi because of its high yield of sweet potato tubers. Both Hwangmi and Sinmi showed very high water content of more than 70 per cent. The highest yield of total solid matter from Sinmi was on September 15 and that from Hwangmi on September 30. Sinmi and Hwangmi harvested on September 30 showed the highest starch content. 4. Variation in water content, starch, soluble sugar, protein and fat content during storage was minimum in the sweet potatoes stored in the temperature controlled storage chamber and was maximum in those stored in the cave under ground. As a result, percent of grade A sweet potatoes remaining was highest in the temperature controlled storage chamber and lowest in the cave under ground. 5. Hwangmi was more susceptible to Rhizopus nigricans than Sinmmi, especially under the humid condition. 6. Shape and thickness of slice did not affect the yield of dried sweet potatoes but affected the time needed for drying. Depending on the thickness and the shape of slice, variations of 36 hours and 12 hours in drying time were observed respectively. Drying table was most effective in drying of sweet potatoes and drying rack was also effective and required 2 days less time than mat. Dipping in water did not show any effect on drying time but improved the color of dried sweet potatoes.

칼라 화상 합성을 위한 윤곽선 추출 고속화 알고리즘에 관한 연구

황인선,김희수,박경우,조정래,위승정 광주보건대학 1998 論文集 Vol.23 No.-

This Paper describes a contour extraction algorithm for color image composition. When a new image is compoended with an object cut from many images, it is important to extract contour f the object. The principal way of extracting contour suggested the method that the contour of an object is drawn with a thick line, and the line is made a thin line by spacial differental value if input image. But in that case, as there is an defect which takes processing the above mentioned method.

皮日休의 文學觀

황정희 中國語文硏究會 1995 中國語文論叢 Vol.8 No.-