Application of heterogeneous RNA probes labeled immuno-fluorescent agent DIG for the screening a noble gene in cucumber

Kim,Dai-Hee,Kim,Byung-Oh,Park,Gyu-Hwan,Takahashi,Hideyuki,Kim,Kyung-Min 한국자원식물학회 2004 Plant Resources Vol.7 No.2

We tried to isolating a noble gene from cucumber library with heterogeneouse RNA probe labeled DIG of Arabidopsis PIN3 gene. Two kinds of RNA probes which had no significant homology each others, were designed from the 5 - and 3 - prime nucleotides of the AtPIN3 gene. In the first and second screenings of the cDNA library of cucumber with the probes, two positive clones were identified with specific duplicate signals. However, we isolated cDNA fragments homologous with putative nucleases from Nicotiana, Arabidopsis, Cordialis, and Oryza sativa, there was no significant homology with any other PIN family genes.

MOSFET를 이용한 2차 대역통과 SCF의 실현에 대한 연구

金圭煥 군산대학교 1989 群山水産專門大學 硏究報告 Vol.23 No.2

Recently, the demand of Band-pass SCFilter has been increased as the circuits of currents Communication systems. But as the most of SCF has been built in complex circuit with many elements, there are necessity to develop effective integrating methods and electric characteristics. In this paper, the effective circuits of SCF realized by MOSFET were studied analytically and its characteristics were considered experimentally. The results were as follows. 1. The gain and selectivity could be controlled easily, because optional value setting of current transmission rate is possible by adoption of MOSFEF current conveyor. 2. The expansion of transmission characteristics by using MOSFET integrator could be possible to get largely. 3. The electrostatic capacity could be reduced fully. 4. The experimental frequency responses of designed SCF were almost similar to that of theoretical value.

二面交雜에 依한 고추 開化所要日數의 遺傭分析

金?椿,朴圭煥 COLLEGE OF AGRICULTURE KYUNGPOOK NATIONAL UNIVERSI 1984 慶北大農學誌 Vol.2 No.-

This study was performed to obtain the basic informations on days to first flower in red pepper. Eight parent varieties and twenty eight F₁ hybrids from 8X8 half diallel cross were used as the materials, and heterosis, combining ability, the degree and direction of dominance, and heritability were evaluated. The 1% significances in day to first flower were observed among parents and F₁s, and the mean of F₁s was not significantly longer than parents. Positive and negative heterosis and heterobeltiosis were observed. Both average heterosis and heterobeltiosis were negative. General combining ability (GCA) and specific combining ability (SCA) variances were highly significant, and GCA variances were greater than SCA variances. Partial dominance was observed in days to first flower and the direction of dominance was negative excluding Chunan and Saegochu varieties. The number of effective gene was estimated as one, and heritabilities in narrow and broad sense were fairly high.

항공기 착륙장치의 충격흡수특성해석

김두만,임경호,황재혁,이환규 한국항공대학교 1992 論文集 Vol.30 No.-

본 연구를 통하여 항공기 착륙장치의 거동(동특성 및 완충기 효율)을 해석하고 평가하는 Software를 개발하엿다. 이 프로그램을 개발하기 위해 착륙장치를 2개의 자유도를 가지는 동역학시스템으로 모델링하고 운동방정식을 유도하였다. 또한 완충기에 작용하는 내력(즉 공기력, orifice force, friction force)을 표현하는 관계식을 정립하였으며, 타이어의 힘을 표현하는 관계식을 유도하였다. 그리고 운동방정식에는 타이어의 Spin Up운동도 고려되었다. 선정된 항공기의 착륙장치의 Parameter의 값을 프로그램의 입력 Data로 사용했을 때 매우 신뢰할 만한 동특성의 결과를 얻었으며, 특히 Drop Test 결과와 본 연구에서 개발된 Software에서 얻어진 결과를 비교했을 때 매우 흡사한 결과를 얻었다. 따라서 본 연구에서 개발된 Software는 앞으로 국산화되는 착륙장치의 개발 및 설계에 매우 유용하게 사용될 것으로 판단된다. 또한 본 연구에서 사용된 입력 Data중 중요한 10개의 Parameter를 선정하여, 각 Parsmeter를 변화시키면서 착륙장치의 동특성 및 효율의 변화를 고찰해 보았다. 완충기 설계시 가장 중요한 Parameter들은 K-factor, Orifice 단면적 등과 같은 완충기의 변수로 사료되며, 타이어의 변수 T??이나 b는 중요한 변수가 아님을 확인하였다. 또한 항공기의 무게 W, Wheel/Tire의 무게, 완충기의 초기 공기압 P??, 항공역학계수 A도 착륙장치의 효율에는 크게 영향을 미치지 않고 있음을 고찰하였다. 그러나 공기실의 Politropic 지수 n, 항공기의 수직착륙속도 V는 착륙장치의 효융에 Non-Monotonic하게 영향을 미치는 것을 고찰하였다. The software which analysis and evaluate the dynamic characteristics of an landing gear has been developed in this study. The landing gear system has been modelled to be 2 DOF dynamic system to drive the equation of motion. The three internal forces, which are air pressure force, orifice force, and friction force, has been formulated and also the tire force has been formulated and also the tire force has been derived. When the typical values of the design parameters is applied to the developed software, the reliable results of the dynamic characteristics are obtained. Furthermore there is a good agreement between the simulation results and experimental drop test extracted from relevant literatures. It can be concluded that the developed software will be useful for the design and development of a landing gear system inside Korea.

저감도 6차 대역통과 SCF의 실현

김규환 群山大學校 情報通信技術硏究所 1998 情報通信技術硏究論文集 Vol.2 No.-

Recently, the demand of Band Pass SC Filter has been increased as the circuits of currents communication system. But as the most SCF has been built in the complex circuits having many resistors, capacitor and switches. There are problems to reduce the cost and the number of elements, also to realize low-sensitivity. In this paper, in order to design simple and economic 6th order Band Pass SC Filter using the SC integrators of operational amplifiers, all the inductances in the 6the LC Band Pass Filter network were replaced by switched-capacitor. And, SC BPF were studied analystically and its characteristics were considered. Also, their results show that the sensitivity and frequency characteristics of designed SC BPF were almost similar to that of the theoretical and experimental values.

專門大學 電子通信 技術人力을 위한 産學協同敎育方案 硏究

金圭煥,高南泳,梁義藏 군산대학교 1987 群山水産專門大學 硏究報告 Vol.21 No.1

In modern industry, the reorganizations of organization and job classification of telecommunication are required for the Advanced Information Society (AIS) which is being accomplished by the entrance of new media, Information Service Noetwork plan for Korean government offices (ISN) and Integrated Services Digital Network (ISDN). Accordingly, the demand of competent technician would be increased for performance of the related job. In this paper, educational system of College-Industry-Korea Vocational Training and Management Agency Cooperation for the telecommunication department was studied for the effective education of the specified technician to be engaged in the specified job. The results were as follows. 1. By the establishment of College-Industry-Korean Voctional Training and Management Agency and their linking operation, the gap in their technical level could be narrowed. In addition, the effective improvement for the field practice, the official approval system, the normalization of the special education and the settlement of its related cooperation business with administrative agencies could be advanced promptly. 2. The industry should take the advancement of its technical level by establishing of employ job for the professional course technician of junior college graduates and by hiring of the 2nd class licensed technician for the related job. 3. Telecommunication department should carried out not only the efficient operation of curriculum (Table 13.) and development of teaching materials fur special technical education according to each course , but also the substantiality of field practice. 4. Korea Vocational Training and Management Agency is desired to develope the problems of examination approached to practical technique level of industry and to improve the applicant qualification, examination date and method of practical examination for normalization of technical education in junior college telecommunication department.

5차 SC 저역통과 필터의 구성

김규환 群山大學校 1996 論文集 Vol.23 No.-

The most SCF has been built complex circuits having many resistors, capacitor and switches. There are problems to reduce the cost and the number of elements, also to make the integrated circuits easily. In this paper, a design method of 5th order SC low pass filter are presented by change of a built 5th LC low Pass Filter with reduced number of operational amplifiers. And, SC LPF were studied analystically and its characteristics were considered experimentally. Also, it shows that frequency characteristics of designed SCF by experiment were almost similar to that of theroretical values.

Current Conveyor를 이용한 2차 대역통과 SCF의 실현

金圭煥 군산대학교 1991 群山水産專門大學 硏究報告 Vol.25 No.2

The most SCF has been built complex circuits having many resistors, capacitors and switches. There are problems to reduce the cost and the number of elements, also to make the integrated circuits easily. In this paper, a design method of 2nd Band Pass SC Filter using only one C.C. built by MOSFET and four capacitors are presented. The characteristics of circuits could be possible to connect circuits without subordinative buffer according to input high impedance of the circuit. And integrated circuit could be made simply and easily because Q of the circuit is decided by ratio of circuit elements. Also, it shows that frequency characteristics of designed SCF by experiment were almost similar to the of theoretical values.

건축공사시방서에서의 KS규격의 국제인증방안에 관한 연구

김경식,이재섭,김용수,이규진,조용훈,최완철,전재열,이현수,구재동,이기환 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1

The purpose of this research is make out construction specifications for International Construction. Using off-shore products incur cost increase and longer delivery term of construction. and inconvenient on International Construction. Expand the range of additional local products conforming to performance. Life Cycle Cost. maintainability. operability. user's acceptability, etc.. And this study is suggest the standard formats and the techniques for construction specification.

水 海洋係 專門大學 通信科 敎育課程 開發 硏究

金永煜,金秀植,高南泳,梁義藏,金圭煥,文建,鄭東洙 군산대학교 1984 群山水産專門大學 硏究報告 Vol.18 No.1

We have studied on the curriculum to develop the communication department of the inter-related fisheries and marine junior college groups and abridged them as follows. (1) A vessel communication has the special characteristics compared with general communication field and also it has an aim to protect the lives and the property from dangerous navigation. For these reasons we must educate many competent graduates who are versed in business and accustom themselves to necessary knowledge of communication and can be adapted them selves to the advanced communication method, techniques and international information on the communication. (2) Training goal and way of thinking of students were rearranged and the occupational cluster in the electronic-communication field was fractionated to develop the curriculum. Especially regarding operator's duty served the above purpose as a occupational cluster, we analyzed their job in accordance with it. (3) International convention on standards of training certification and watchkeeping for seafarers, 1978 was reflected, also international telecommunication convention was referred so that it might be satisfied with the condition to be observed. (4) Developing the curriculum, we responed and analyzed the questions from scientific world, industrial organizations and graduates and provide their advice and suggestions form consultive committee and reflected them to develop the curriculum