Research and Development of Superhard Cutting Database

HuiPing Zhang,WenJuan Zheng,YiNan Lai,Mingna Ding,Zhen Chen 보안공학연구지원센터 2014 International Journal of Hybrid Information Techno Vol.7 No.5

The Role of Wheat Germ Agglutinin in the Attachment of Pseudomonas sp. WS32 to Wheat Root

Jian Zhang,Liyuan Meng,Yuanyuan Cao,Huiping Chang,Zhongyou Ma,Leni Sun,Ming Zhang,Xinyun Tang 한국미생물학회 2014 The journal of microbiology Vol.52 No.12

Wheat germ agglutinin (WGA), which is secreted on thesurface of wheat root, has been defined as a protein that reversiblyand non-enzymatically binds to specific carbohydrates. However, little attention has been paid to the functionof WGA in the attachment of bacteria to their host plants. The aim of this study was to investigate the role of WGA inthe attachment of Pseudomonas sp. WS32 to wheat roots. Wheat roots were initially treated with double-distilled water,WGA-H (WGA solution that was heated at 100°C for 15 min)and WGA, independently. Subsequently, the roots were coincubatedwith cell solutions (109 cells/ml). A dilution platemethod using a solid nutrient medium was employed to determinethe adsorption of WS32 to wheat roots. WGA waslabeled with fluorescein isothiocyanate and detected usingthe fluorescent in situ hybridization (FISH) technique. Thenumber of adsorptive WS32 cells on wheat roots was significantlyincreased when the wheat roots were pretreatedwith WGA, compared with the control treatment (p = 0.01). However, WGA-H failed to increase the amount of bacterialcells that attached to the wheat roots because of the lossof its physiological activity. The FISH assay also revealedthat more cells adhered to WGA-treated wheat roots than tocontrol or WGA-H-treated roots. The results indicated thatWGA can mediate Pseudomonas strain WS32’s adherenceto wheat seedling roots. The findings of this study provide abetter understanding of the processes involved in plant-microbe interactions.

Physical, Chemical Properties and Structural Changes of Zaodan Pickled by Vacuum Decompression Technology

Naxin Sun,Huiping Liu,Xiaowei Zhang,Hongni Wang,Shaojuan Liu,Pei Chen,Weijie Yu,Kai Liu 한국축산식품학회 2018 한국축산식품학회지 Vol.38 No.2

To shorten the production cycle of Zaodan, this study first pickled Zaodan by a novel technology -vacuum decompression technology. Vacuum decompression technology could reduce the pickling time of Zaodan from 20wkto about 9wk.The protein content, moisture and pH of the Zaodan egg white gradually decreased with a concomitant increase in salt during the pickling process. The total sulfhydryl group (SH) group content of the egg white proteins was increased to 2.43×10-3mol/L after being pickled for 30 d, whereas the content of disulphide bonds (SS) was reduced to 23.35×10-3mol/L. The surface hydrophobicity was lowest after pickling for 30 d. In addition, great changes occurred in the secondary structure of the egg white proteins after pickling for 20 d. The disappearance of ovomucin was noticeable based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis.

Next-generation sequencing for the genetic characterization of Maedi/Visna virus isolated from the northwest of China

Ling Zhao,Liang Zhang,Xiaona Shi,Xujie Duan,Huiping Li,Shuying Liu 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.6

Background: Maedi/Visna virus (MVV) is a contagious viral pathogen that causes considerable economic losses to the sheep industry worldwide. Objectives: In China, MVV has been detected in several regions, but its molecular characteristics and genetic variations were not thoroughly investigated. Methods: Therefore, in this study, we conducted next-generation sequencing on an MVV strain obtained from northwest China to reveal its genetic evolution via phylogenetic analysis. Results: A MVV strain obtained from Inner Mongolia (NM) of China was identified. Sequence analysis indicated that its whole-genome length is 9193 bp. Homology comparison of nucleotides between the NM strain and reference strains showed that the sequence homology of gag and env were 77.1%–86.8% and 67.7%–75.5%, respectively. Phylogenetic analysis revealed that the NM strain was closely related to the reference strains isolated from America, which belong to the A2 type. Notably, there were 5 amino acid insertions in variable region 4 and a highly variable motif at the C-terminal of the surface glycoprotein (SU5). Conclusions: The present study is the first to show the whole-genome sequence of an MVV obtained from China. The detailed analyses provide essential information for understanding the genetic characteristics of MVV, and the results enrich the MVV library.

Least squares estimation of kinetic parameters in batch adsorption of phenol with confidence interval analysis

Ying Yan,Yan Shao,Huiping Zhang 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.8

Adsorption kinetics of phenol on granular coconut shell activated carbon and granular coal based activated carbon was investigated by the model of homogeneous surface diffusion plus external film mass transfer with the stirred batch adsorber. The model was solved numerically by finite element and the parameter estimation was performed with the nonlinear least squares method. Through the confidence interval analysis and evaluation of the error sum of squares,higher precision of the parameter estimates can be obtained by combining more decay curves; the decay curves with the different initial solution concentrations and adsorbent dosages can be well represented with identical film mass transfer coefficient and surface diffusivity, and the effect of surface adsorption coverage on the surface diffusivity is negligible in the adsorption systems. The values of the film mass transfer coefficient and surface diffusivity are in the order of magnitude 10−5 and 10−12, respectively.

Preparation of Titanium Microfiltration Membrane by Field-flow Fractionation Deposition

Wang QiangBing,Tang HuiPing,Zhang QianCheng,Qiu QunFeng,Wang JianYong 한국분말야금학회 2006 한국분말야금학회 학술대회논문집 Vol.2006 No.1

The primary aim pursued by the preparation of separation membrane is the preparation of the membrane thin as well as with no defect. The field-flow fractionation deposition is a new molding technology which can overcome the traditional disadvantages such as multi-preparation to the preparation of great area of separation membrane with no defect. Therefor the mainly ingredients which influence the appearance and performance of titanium membrane layer are investigated by scanning electricity mirror (SEM) as well as porous material testing instrument: powder performance prepared and confected; selection of supporting body; sintering system such as temperature and time. It is shown that the membrane thickness can be controlled at or so; the filtration precision mainly rests with powder performance and selection of supporting body and little sintering system

Catalytic activity of copper-ceria catalysts supported on different zeolites for CO oxidation

Ying Yan,Dong Zhang,Huiping Zhang 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.6

Copper-ceria catalysts for CO oxidation supported on 4A, 5A, NaX and NaY zeolites were prepared by incipient wetness impregnation and excess-solution impregnation. Catalysts were characterized by SEM, EDX, XRD, N2 adsorption-desorption, H2-TPR and XPS. Results revealed that the catalysts were greatly affected by zeolites and preparation method. EDX results indicated the metal loading of 4A-ES (5.1 wt% Cu, 15.7 wt% Ce), 5A-ES (5.9 wt% Cu, 19.2% Ce), NaX-ES (11.7wt% Cu, 4.2 wt% Ce) and NaY-ES (11.0 wt% Cu, 7.9 wt% Ce) greatly varied. TPR results suggested that the peak at around 195 oC was presented in NaX-ES and 4A-IW, standing for dispersed copper species that is very active for CO oxidation. The catalytic activity of 4A-ES and NaX-ES was the best among catalysts made by excess-solution impregnation, demonstrated by the lowest T50 at 127 and 129 oC, respectively. The catalytic activity of catalysts made by incipient wetness impregnation was worse than that of catalysts made by excess-solution impregnation, examined by the T50 of 4A-IW and NaX-IW at 128 and 192 oC, respectively.

Identifification and development of a core set of informative genic SNP markers for assaying genetic diversity in Chinese cabbage

Peirong Li,Tongbing Su,Shuancang Yu,Huiping Wang,Weihong Wan,Yangjun Yu,Deshuang Zhang,Xiuyun Zhao,Changlong Wen,Fenglan Zhang 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.3

Rapid, economical, and reliable genotyping is an important requirement for germplasm analysis and cultivar identifi cationin crop species. Chinese cabbage ( Brassica rapa L. subsp. pekinensis (Lour.) Hanelt) originated in China and is now aneconomically important vegetable crop worldwide, especially in East Asia. In this study, we evaluated 1167 single nucleotidepolymorphisms (SNPs) among 166 representative Chinese cabbage inbred lines using a KASP genotyping assay. Onthe basis of polymorphisms and principal component analysis, we selected 60 core SNPs distributed on all Brassica rapachromosomes with allele frequencies suffi ciently balanced so as to provide adequate information for genetic identifi cation. The core set of SNPs was used for construction of a neighbor-joining dendrogram, in which the 166 inbred lines wereclustered into spring, summer, and autumn ecotype groups. Clustering of the ecotype groups was better resolved than thatachieved with 1167 and 360 polymorphic SNP datasets. Stability and resolution of the core SNP markers were tested using178 commercial hybrid Chinese cabbage cultivars to confi rm their utility in genetic identifi cation. The set of 60 informativeand stable SNP markers showed high discriminatory power and relatively uniform genomic distribution (4–9 markers perchromosome). The SNPs represent a cost-effi cient and accurate marker set for germplasm analysis and cultivar identifi cationand are suitable for molecular marker-assisted breeding in Chinese cabbage.

A GA-Based Neural Fuzzy System for Modeling a Paper Mill Wastewater Treatment Process

Huang, Mingzhi,Wan, Jinquan,Ma, Yongwen,Zhang, Huiping,Wang, Yan,Wei, Chaohai,Liu, Hongbin,Yoo, ChangKyoo American Chemical Society 2011 INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH - Vol.50 No.23

<P>A genetic algorithm-based neural fuzzy system (GA-NFS) was presented for studying the coagulation process of wastewater treatment in a paper mill. In order to adapt the system to a variety of operating conditions and acquire a more flexible learning ability, the GA-NFS was employed to model the nonlinear relationships between the effluent concentration of pollutants and the chemical dosages, and a hybrid learning algorithm divided into two stages was proposed for parameters learning. During the first learning stage, a genetic algorithm was used to optimize the structure of GA-NFS and the membership function of each fuzzy term due to its capability of parallel and global search. On the basis of an optimized training stage, the back-propagation algorithm (BP algorithm) was chosen to update the parameters of GA-NFS to improve the system precision. The GA-NFS proves to be very effective in modeling coagulation perform and performs better than adaptive-network-based fuzzy inference system (ANFIS). RMSE, MAPE, and <I>R</I> between the predicted and observed values for GA-NFS were only 0.01099, 2.3337, and 0.9375, respectively.</P>

Pretreatments of Broussonetia papyrifera: in vitro assessment on gas and methane production, fermentation characteristic, and methanogenic archaea profile

Dong Lifeng,Gao Yanhua,Jing Xuelan,Guo Huiping,Zhang Hongsen,Lai Qi,Diao Qiyu 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.9

Objective: The present study was conducted to examine the gas production, fermentation characteristics, nutrient degradation, and methanogenic community composition of a rumen fluid culture with Broussonetia papyrifera (B. papyrifera) subjected to ensiling or steam explosion (SE) pretreatment. Methods: Fresh B. papyrifera was collected and pretreated by ensiling or SE, which was then fermented with ruminal fluids as ensiled B. papyrifera group, steam-exploded B. papyrifera group, and untreated B. papyrifera group. The gas and methane production, fermentation characteristics, nutrient degradation, and methanogenic community were determined during the fermentation. Results: Cumulative methane production was significantly improved with SE pretreatment compared with ensiled or untreated biomass accompanied with more volatile fatty acids production. After 72 h incubation, SE and ensiling pretreatments decreased the acid detergent fiber contents by 39.4% and 22.9%, and neutral detergent fiber contents by 10.6% and 47.2%, respectively. Changes of methanogenic diversity and abundance of methanogenic archaea corresponded to the variations in fermentation pattern and methane production. Conclusion: Compared with ensiling pretreatment, SE can be a promising technique for the efficient utilization of B. papyrifera, which would contribute to sustainable livestock production systems. Objective: The present study was conducted to examine the gas production, fermentation characteristics, nutrient degradation, and methanogenic community composition of a rumen fluid culture with Broussonetia papyrifera (B. papyrifera) subjected to ensiling or steam explosion (SE) pretreatment.Methods: Fresh B. papyrifera was collected and pretreated by ensiling or SE, which was then fermented with ruminal fluids as ensiled B. papyrifera group, steam-exploded B. papyrifera group, and untreated B. papyrifera group. The gas and methane production, fermentation characteristics, nutrient degradation, and methanogenic community were determined during the fermentation.Results: Cumulative methane production was significantly improved with SE pretreatment compared with ensiled or untreated biomass accompanied with more volatile fatty acids production. After 72 h incubation, SE and ensiling pretreatments decreased the acid detergent fiber contents by 39.4% and 22.9%, and neutral detergent fiber contents by 10.6% and 47.2%, respectively. Changes of methanogenic diversity and abundance of methanogenic archaea corresponded to the variations in fermentation pattern and methane production.Conclusion: Compared with ensiling pretreatment, SE can be a promising technique for the efficient utilization of B. papyrifera, which would contribute to sustainable livestock production systems.