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RISS 인기검색어
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- 저자 : Yangjun Yu (검색결과 3 건)
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Shuancang Yu,Xiaoyi Wang,Xiuyun Zhao,Deshuang Zhang,Yangjun Yu,Jiabing Xu,Fenglan Zhang 한국유전학회 2010 Genes & Genomics Vol.32 No.5
Pak‐choi (Brassica rapa L. ssp. chinensis Makino) is one of the most important vegetable, especially in southern China. Morphological traits of Pak‐choi vary widely, and the genetic basis of these variations is largely undescribed. In this study,we evaluated eighty Pak‐choi accessions for genetic diversity based on 29 morphological descriptors and 20 microsatellite (SSR) loci. Our data reveal a high level of diversity in this set of Pak‐choi accessions. Euclidean distance was used for cluster analysis. The UPGMA dendrogram grouped the Pakchoi accessions into five major clusters based on morphological characteristics. As expected, similar morphological characteristics grouped together in cluster branches. Further,population structure was explored to identify subpopulations based on allele frequency. Five groups were identified, and showed considerable correlation with the morphological clusters. Associations between SSR markers and agronomic traits were further investigated using a non‐parametric Kruskal–Wallis K‐test and six additional parametric models. Significant associations were found between 18 SSR loci and 28 morphological traits. This study provides evidence that marker‐trait associations can be validated in segregating populations and exploited through marker‐assisted selection.
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Shengju Zhang,Shuancang Yu,Fenglan Zhang,Longting Si,Yangjun Yu,Xiuyun Zhao,Deshuang Zhang,Weihong Wang 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.5
Downy mildew, caused by Hyaloperonospora parasitica, is a destructive disease of the Chinese cabbage (Brassica rapa ssp. pekinensis). A rapid resistance/susceptibility test for H. parasitica was established by the inoculation of a leaf disk test. Four conditions were tested and the optimal condition was found when the inoculated leaf disk was placed into dark conditions at 16℃ for the first 24 hours after inoculation, followed by 5 days of light at 20℃and 23℃ for 4 hours and 5 hours, respectively, dark at 16℃ and 12℃ for 3 hours and 12 hours, respectively, and a final temperature of 16℃ for 24 hours. There was a good correlation between the resistance levels of leaf disks and the resistance of seedlings or adult plants, which indicated that testing leaf disks should be the preferred methods to predict the resistance of adult plants. Using this method, downy mildew resistance was investigated in a double haploid (DH) population at four developmental stages. The results showed that the genetic pattern, which was deduced from the DH segregation, was relatively similar, but varied slightly during plant development.
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Peirong Li,Tongbing Su,Shuancang Yu,Huiping Wang,Weihong Wan,Yangjun Yu,Deshuang Zhang,Xiuyun Zhao,Changlong Wen,Fenglan Zhang 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.3
Rapid, economical, and reliable genotyping is an important requirement for germplasm analysis and cultivar identifi cationin crop species. Chinese cabbage ( Brassica rapa L. subsp. pekinensis (Lour.) Hanelt) originated in China and is now aneconomically important vegetable crop worldwide, especially in East Asia. In this study, we evaluated 1167 single nucleotidepolymorphisms (SNPs) among 166 representative Chinese cabbage inbred lines using a KASP genotyping assay. Onthe basis of polymorphisms and principal component analysis, we selected 60 core SNPs distributed on all Brassica rapachromosomes with allele frequencies suffi ciently balanced so as to provide adequate information for genetic identifi cation. The core set of SNPs was used for construction of a neighbor-joining dendrogram, in which the 166 inbred lines wereclustered into spring, summer, and autumn ecotype groups. Clustering of the ecotype groups was better resolved than thatachieved with 1167 and 360 polymorphic SNP datasets. Stability and resolution of the core SNP markers were tested using178 commercial hybrid Chinese cabbage cultivars to confi rm their utility in genetic identifi cation. The set of 60 informativeand stable SNP markers showed high discriminatory power and relatively uniform genomic distribution (4–9 markers perchromosome). The SNPs represent a cost-effi cient and accurate marker set for germplasm analysis and cultivar identifi cationand are suitable for molecular marker-assisted breeding in Chinese cabbage.
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