Application and Analysis of Rhizopus oryzae Mycelia Extending Characteristic in Solid-state Fermentation for Producing Glucoamylase

( Xianghua Tang ),( Tianbao Luo ),( Xue Li ),( Huanhuan Yang ),( Yunjuan Yang ),( Junjun Li ),( Bo Xu ),( Zunxi Huang ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.11

Enhanced application of solid-state fermentation (SSF) in industrial production and the influence of SSF of Rhizopus K1 on glucoamylase productivity were analyzed using the flat band method. A growth model was implemented through SSF of Rhizopus K1 in this experiment, and spectrophotometric method was used to determine glucoamylase activity. Results showed that in bran and potato culture medium with 70% moisture in a loose state, μ of mycelium reached to 0.15 h^-1 after 45 h of culture in a thermostatic water bath incubator at 30°C. Under a low-magnification microscope, mycelial cells appeared uniform, bulky with numerous branches, and were not easily ruptured. The generated glucoamylase activity reached to 55 U/g (dry basis). This study has good utilization value for glucoamylase production by Rhizopus in SSF.

Anti-Inflammatory Activity of Epimedium brevicornu Maxim Ethanol Extract

Shan Huang,Ning Meng,Bingquan Chang,Xianghua Quan,RuiYing Yuan,Bin Li 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.7

Epimedium brevicornu Maxim has been used as a traditional herbal drug in China. In this study, the anti-inflammatory effects of E. brevicornu Maxim ethanol extract (EBME) were investigated in RAW264.7 macrophages and mice challenged with lipopolysaccharide (LPS). Results showed that EBME attenuated inflammation by decreasing the production of several proinflammatory mediators, such as nitric oxide (NO), prostaglandin (PG) E2, inducible nitric oxide synthase, and cyclooxygenase-2, in LPS-stimulated RAW264.7 macrophages. EBME increased the expression of heme oxygenase-1 (HO-1) and promoted the nuclear translocation of nuclear factor erythroid 2-related factor 2. The inhibitory effects of EBME on LPS-stimulated NO and PGE2 expression were partially reversed by HO-1 inhibitor. EBME also elicited an anti-inflammatory effect by inhibiting the production of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 in LPS-induced peritonitis. Therefore, EBME exhibited anti-inflammatory effects in vitro and in vivo.

Photocatalytic removal of cefazolin using Ag3PO4/BiOBr under visible light and optimization of parameters by response surface methodology

Yuhui Xiao,Xianghua Song,Zhuo Liu,Ruiping Li,Xiaorong Zhao,Yingping Huang 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.45 No.-

The degradation of cefazolin (CFZ) by Ag3PO4/BiOBr composites under visible-light irradiation wasexplored. The main and interaction of parameters (catalyst dosage, pH, CFZ initial concentration anddegradation time) on removal of CFZ were studied by Box–Behnken design combined with responsesurface methodology. The pH was the most influential factor and both h+ and OH played a role in thephotocatalytic process. The high correlation coefficients (R2 = 0.9986 and adjusted R2 = 0.9973)demonstrated closefit between the predicted and experimental values. The exceptional efficiency ofAg3PO4/BiOBr composite in removing CFZ represents a promising technique for treatment of CFZcontainingwastewater.

E-business Ecosystem and its Evolutiionary Path

Ganglan Hu,Xianghua Lu,Lihua Huang 인하대학교 정석물류통상연구원 2009 인하대학교 정석물류통상연구원 학술대회 Vol.2009 No.10

The phenomenon of e-business industrial cluster in China has been emerging. Despite the growth in research on the benefits of industrial cluster, our understanding of the process forming the cluster is limited. the study attempts to fill the gap by answering “How has e-business industrial cluster in China been formed?” and “ what characterize it?” Applying business ecosystem theory as the theoretical lens, the concept of e-business ecosystem was pointed out first time in this paper to describe th phenomenon of e-business industrial cluster. A case study of Alibaba Group was undertaken to investigate the process that a typical e-business ecosystem in China got shape, developed, and matured. By analyzing the findings from the case study, four stages during e-business ecosystem’s evolutionary path and its characteristics are identified. The study has implications for both researchers and practitioners, and valuable for understanding the development of e-business industry in china.

Characterization of a Family 3 Polysaccharide Lyase with Broad Temperature Adaptability, Thermo-alkali Stability, and Ethanol Tolerance

JunPei Zhou,Yanyan Dong,Yajie Gao,Xianghua Tang,Junjun Li,YUN-JUAN YANG,Bo Xu,Zhenrong Xie,Zunxi Huang 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.4

The 774-bp pectate lyase gene plyAI4 from Bacillus sp. I4 was cloned and expressed in E. coli. The gene encodes a 257-residue polypeptide (PlyAI4, 28.3 kDa)with the highest identities of 97.3% with a putative pectate lyase from Bacillus subtilis BSn5 (ADV94306) and 60.3%with an identified pectate lyase of the polysaccharide lyase family (PL) 3 from Paenibacillus amylolyticus 27C64(ADB78774). The purified recombinant PlyAI4 (rPlyAI4)exhibited apparently optimal activity at pH 10.5 ~ 11.0 and 50oC. Compared with the majority of reported alkaline pectate lyases, rPlyAI4 exhibited more residual enzyme activity at 20oC (~45%) or at 70oC (~50%) and better thermostability at 70oC (~60 min half-life at 70oC). In the presence of 20% (v/v) ethanol, pectate lyase activity was enhanced by 0.2 fold. After incubation in 40% (v/v)ethanol at 37oC and pH 8.5 for 1 h, the purified rPelAI4retained more than 75% of the initial activity. Sequence analysis proposed a new signature block, A-D-G-[V/I]-H,for PL 3 pectate lyases. These properties may prove to be important with regards to PlyAI4 for basic research and industrial application.

Cloning, Heterologous Expression, and Characterization of Novel Protease- Resistant α-Galactosidase from New Sphingomonas Strain

( Jun Pei Zhou ),( Yan Yan Dong ),( Jun Jun Li ),( Rui Zhang ),( Xianghua Tang ),( Yuelin Mu ),( Bo Xu ),( Qian Wu ),( Zun Xi Huang ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.11

The α-galactosidase-coding gene agaAJB13 was cloned from Sphingomonas sp. JB13 showing 16S rDNA (1,343 bp) identities of ≤97.2% with other identified Sphingomonas strains. agaAJB13 (2,217 bp; 64.9% GC content) encodes a 738-residue polypeptide (AgaAJB13) with a calculated mass of 82.3 kDa. AgaAJB13 showed the highest identity of 61.4% with the putative glycosyl hydrolase family 36 α-galactosidase from Granulicella mallensis MP5ACTX8 (EFI56085). AgaAJB13 also showed <37% identities with reported protease-resistant or Sphingomonas α-galactosidases. A sequence analysis revealed different catalytic motifs between reported Sphingomonas α-galactosidases (KXD and RXXXD) and AgaAJB13 (KWD and SDXXDXXXR). Recombinant AgaAJB13 (rAgaAJB13) was expressed in Escherichia coli BL21 (DE3). The purified rAgaAJB13 was characterized using p-nitrophenyl-α-D-galactopyranoside as the substrate and showed an apparent optimum at pH 5.0 and 60oC and strong resistance to trypsin and proteinase K digestion. Compared with reported proteaseresistant α-galactosidases showing thermolability at 50oC or 60oC and specific activities of <71 U/mg with or without protease treatments, rAgaAJB13 exhibited a better thermal stability (half-life of >60 min at 60oC) and higher specific activities (225.0-256.5 U/mg). These sequence and enzymatic properties suggest AgaAJB13 is the first identified and characterized Sphingomonas α-galactosidase, and shows novel protease resistance with a potential value for basic research and industrial applications.

Alternative Biological Material for Tissue Engineering of the Vagina: Porcine-Derived Acellular Vaginal Matrix

Tian Yanpeng,Liu Yibin,Xiao Yanlai,Li Zhongkang,Zhang Mingle,Chen Liang,Li Zhen,Zhang Wangchao,Zhang Zhiqiang,Kong Desheng,Meng Li,Du Yanfang,Zhang Jingkun,Gao Jingui,Huang Xianghua 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.2

Background: Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome is a severe congenital disorder characterized by vaginal hypoplasia caused by dysplasia of the Müllerian duct. Patients with MRKH syndrome often require nonsurgical or surgical treatment to achieve satisfactory vaginal length and sexual outcomes. The extracellular matrix has been successfully used for vaginal reconstruction. Methods: In this study, we developed a new biological material derived from porcine vagina (acellular vaginal matrix, AVM) to reconstruct the vagina in Bama miniature pigs. The histological characteristics and efficacy of acellularization of AVM were evaluated, and AVM was subsequently transplanted into Bama miniature pigs to reconstruct the vaginas. Results: Macroscopic analysis showed that the neovaginas functioned well in all Bama miniature pigs with AVM implants. Histological analysis and electrophysiological evidence indicated that morphological and functional recovery was restored in normal vaginal tissues. Scanning electron microscopy showed that the neovaginas had mucosal folds characteristics of normal vagina. No significant differences were observed in the expression of CK14, HSP47, and α-actin between the neovaginas and normal vaginal tissues. However, the expression of estrogen receptor (ER) was significantly lower in the neovaginas than in normal vaginal tissues. In addition, AVM promoted the expression of β-catenin, c-Myc, and cyclin D1. These results suggest that AVM might promotes vaginal regeneration by activating the β-catenin/c-Myc/cyclin D1 pathway. Conclusion: This study reveals that porcine-derived AVM has potential application for vaginal regeneration. Background: Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome is a severe congenital disorder characterized by vaginal hypoplasia caused by dysplasia of the Müllerian duct. Patients with MRKH syndrome often require nonsurgical or surgical treatment to achieve satisfactory vaginal length and sexual outcomes. The extracellular matrix has been successfully used for vaginal reconstruction. Methods: In this study, we developed a new biological material derived from porcine vagina (acellular vaginal matrix, AVM) to reconstruct the vagina in Bama miniature pigs. The histological characteristics and efficacy of acellularization of AVM were evaluated, and AVM was subsequently transplanted into Bama miniature pigs to reconstruct the vaginas. Results: Macroscopic analysis showed that the neovaginas functioned well in all Bama miniature pigs with AVM implants. Histological analysis and electrophysiological evidence indicated that morphological and functional recovery was restored in normal vaginal tissues. Scanning electron microscopy showed that the neovaginas had mucosal folds characteristics of normal vagina. No significant differences were observed in the expression of CK14, HSP47, and α-actin between the neovaginas and normal vaginal tissues. However, the expression of estrogen receptor (ER) was significantly lower in the neovaginas than in normal vaginal tissues. In addition, AVM promoted the expression of β-catenin, c-Myc, and cyclin D1. These results suggest that AVM might promotes vaginal regeneration by activating the β-catenin/c-Myc/cyclin D1 pathway. Conclusion: This study reveals that porcine-derived AVM has potential application for vaginal regeneration.