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Visual Metonymy and Its Communicative Functions: With the 2019 Oscar Movie Green Book
Hongwei Liu,Youngju Choi 현대문법학회 2021 현대문법연구 Vol.109 No.-
Hongwei Liu and Youngju Choi. 2021. Visual Metonymy and Its Communicative Functions: With the 2019 Oscar Movie Green Book. Studies in Modern Grammar 109, 63-81. Littlemore (2015) claims that metonymy can perform a wide range of communicative functions in different forms and modes of expressions. This paper examines the visual metonymies observed in Green Book, the 2019 Best Picture Oscar Academy Award-winner, elucidating how the movie integrates its narrative with visual metonymy to express abstract and profound themes. Exploring various types of visual metonymy utilized in this movie, this paper reveals how the visual metonymy contributes to the communication effect of a movie. The paper also demonstrates that analysis of visual metonymy in movies enriches the current research on both visual metonymy and movie studies.
Xiaomin Liu,Chengbin Yang,Jing Liu,Jianwei Liu,Rui Hu,Hongwei Lian,Guimiao Lin,Liwei Liu,Ken-Tye Yong,Ling Ye 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4
The optimization of aldose reductase (AR) expression levels and tracking of the AR expression sites within the cell is an essential step in developing a platform for the effective production of aldose reductase inhibitors (ARIs). In this study, we have demonstrated the use of both immunocytochemistry and quantum dots-based immunofluorescence techniques for observing and detecting the expression level and localization of AR in the cytoplasm and cell membrane of a eukaryotic cell model with high levels of AR protein expression. Our results show that high expression levels of human AR can be achieved using the eukaryotic cell model that we have developed. The overexpressed AR can be used for translational studies of hAR and the screening of ARIs. More importantly, the use of the established quantum dots-based immunofluorescence technique in the intracellular labeling of AR allows the determination of the expression and distribution of the AR gene. Overall, the use of the interdisciplinary approach of both genetic engineering and quantum dot-based immunofluorescence allows not only the effective production of a desired protein, but also the determination of the cellular localization of such an expressed protein.
The Specific Case Analysis of Biomineralization Induced by Sulfate Reducing Bacteria
( Hongwei Liu ),( Shuang Qin ),( Chaoyang Fu ),( Fei Xiao ),( Deli Wang ),( Xia Han ),( Tianli Wang ),( Hongfang Liu ) 한국부식방식학회(구 한국부식학회) 2017 Corrosion Science and Technology Vol.16 No.6
The effects of sulfate reducing bacteria (SRB) on the corrosion and scaling of the Q235 carbon steel has been investigated in the simulated sewage water and oil field gathering pipelines production water, using scanning electron microscopy (SEM), energy dispersive x-ray spectrometry (EDS), and three-dimensional stereoscopic microscope. Results indicated that the concentration of SRB reached the maximum value on the ninth day in simulated sewage water with a large amount of scaling on the surface of specimen. In oil field gathering pipelines, a large amount of scaling and mineralization of mineral salts and thick deposition of extracellular polymeric substance (EPS) layers were also observed on the surface of specimen. The thickness of biofilm was about 245 μm within 30 days. After adding microbicides, the thickness of corrosion products film was only up to 48 - 106 μm within 30 days, suggesting that SRB could induce biomineralization. Under-deposit corrosion morphology was uniform in the absence of microbicides while local corrosion was observed in the presence of microbicides.
Genomics and LC-MS Reveal Diverse Active Secondary Metabolites in Bacillus amyloliquefaciens WS-8
Hongwei Liu,Yana Wang,Qingxia Yang,Wenya Zhao,Liting Cui,Buqing Wang,Liping Zhang,Huicai Cheng,Shuishan Song,Liping Zhang 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.3
Bacillus amyloliquefaciens is an important plant disease-preventing and growth-promoting microorganism. B. amyloliquefaciens WS-8 can stimulate plant growth and has strong antifungal properties. In this study, we sequenced the complete genome of B. amyloliquefaciens WS-8 by Pacific Biosciences RSII (PacBio) Single Molecule Real-Time (SMRT) sequencing. The genome consists of one chromosome (3,929,787 bp) and no additional plasmids. The main bacteriostatic substances were determined by genome, transcriptome, and mass spectrometry data. We thereby laid a theoretical foundation for the utilization of the strain. By genomic analysis, we identified 19 putative biosynthetic gene clusters for secondary metabolites, most of which are potentially involved in the biosynthesis of numerous bioactive metabolites, including difficidin, fengycin, and surfactin. Furthermore, a potential class II lanthipeptide biosynthetic gene cluster and genes that are involved in auxin biosynthesis were found. Through the analysis of transcriptome data, we found that the key bacteriostatic genes, as predicted in the genome, exhibited different levels of mRNA expression. Through metabolite isolation, purification, and exposure experiments, we found that a variety of metabolites of WS-8 exert an inhibitory effect on the necrotrophic fungus Botrytis cinerea, which causes gray mold; by mass spectrometry, we found that the main substances are mainly iturins and fengycins. Therefore, this strain has the potential to be utilized as an antifungal agent in agriculture.
Xufang Shen,Hongwei Yan,Lei Zhang,Zhen Yuan,Wenlei Liu,Yumeng Wu,Qi Liu,Xiaoyi Luo,Ying Liu 한국유전학회 2020 Genes & Genomics Vol.42 No.4
Background Quantification of mRNAs in gonads and other tissues at the early critical development stage of sex differentiation may help to provide a global view of regulatory mechanisms underlying sex differentiation. We have recently reported the transcriptomic profiling of fugu gonad associated with sex differentiation. Objectives This study attempted to identify the genes in the brain that are involved in gonadal differentiation and development. Methods In this study, a transcriptomic scan of potential candidate genes involved in sex differentiation was conducted in the brains of fugu larvae at 30 and 40 dah (morphological gonadal sex differentiation had not yet occurred). The dimorphic expression patterns of several candidate genes were verified using quantitative PCR. Results A total of 28.24 Gb of clean reads were obtained and 22,337 genes were identified in the brains of fugu larvae. These included 1008 novel genes that provide abundant data for functional analysis of sex differentiation. 229 genes were identified in the 30 dah larvae that were abundant in the XY brain and 21 that were abundant in the XX brain. In the 40 dah larvae, 325 genes were identified abundant in the XY brain and 174 were identified abundant in the XX brain. Conclusion This is the first investigation into the transcriptome of the fugu larvae brain at the early sex differentiation stage. The results obtained here will enhance the understanding of molecular mechanisms that underly fugu sex differentiation.
Liu, Tongfa,Kim, Dongcheon,Han, Hongwei,Mohd Yusoff, Abd. Rashid bin,Jang, Jin The Royal Society of Chemistry 2015 Nanoscale Vol.7 No.24
<P>Simplifying the process of fine-tuning the electronic and optical properties of graphene oxide (GO) is of importance in order to fully utilize it as the hole interfacial layer (HIL). We introduced silver trifluoromethanesulfonate (AgOTf), an inorganic chemical dopant, that tunes and controls the properties of single-layered GO films synthesized by chemical vapor deposition. The morphology, work function, mobility, sheet resistance, and transmittance of the GO film were systematically tuned by various doping concentrations. We further developed a solution-processable low-temperature hole interfacial layer (HIL) poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (</P>
Distance Measurement of Zooming Image for a Mobile Robot
Hongwei Gao,Jinguo Liu,Yang Yu,Yangmin Li 제어·로봇·시스템학회 2013 International Journal of Control, Automation, and Vol.11 No.4
Aiming at the application of zooming image distance measurement to mobile robot, the related key technologies are investigated in detail in this paper. Firstly, camera parameter calibration is conducted. The camera focus, optical center displacement between two foci, principal point and aberration coefficients are calculated accurately. Then, robust feature matching based on SIFT is realized by the geometrical constraint of a zooming image. Finally, the 3D reconstruction model of zooming image is established. The experimental results based on real sample images validate the practicability of the related algorithms.
HONGWEI CHE,AIFENG LIU,XIAOLIANG ZHANG,HONGJIANG HE,JUNXIAN HOU,JINGBO MU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.7
In this paper, three-dimensional (3D) Co3O4 °ower-like microspheres have been successfully synthesized via a facile ethylene glycol (EG)-mediated solvothermal method followed by calcination. The as-prepared °ower-like precursors microspheres are formed from the assembly of 2D nanosheets in the presence of hexadecyltrimethylammonium bromide (CTAB). The °ower-like architectures of the prepared precursors could be tailored by changing the amount of CTAB. Furthermore, when evaluated as a gas sensor, the obtained Co3O4 °ower-like microspheres exhibit a good response and sensitivity toward ethanol gas, suggesting their promising potential for gas sensors application.