Design of Real-Time Monitoring System on Raw Milk Transport Process

Hongmin Sun,Guihua Jiang,Qingming Kong,Zhongqiu Chen,Xiaoming Li 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.4

With a purpose to guarantee the quality safety of raw milk during the transport process, based on the internet of things, this paper made the raw milk transport process as the research objective. As the key technologies like GPS, GPRS and RFID were applied and STM32F103 Singlechip was adopted to fulfill the hardware design of system. Real-time collection and storage of the information on transport vehicle location and milk box temperature as well as real-time monitoring on raw milk transport process could be conducted to achieve the visualization of the whole process from livestock farm/milk station to processing factory, thus the transparency of raw milk in transportation could be ensured, which provided guarantees for raw milk quality and safety and was of great significance for the healthy development of the dairy products- business in our country.

A simple data assimilation method to improve atmospheric dispersion based on Lagrangian puff model

Li, Ke,Chen, Weihua,Liang, Manchun,Zhou, Jianqiu,Wang, Yunfu,He, Shuijun,Yang, Jie,Yang, Dandan,Shen, Hongmin,Wang, Xiangwei Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.7

To model the atmospheric dispersion of radionuclides released from nuclear accident is very important for nuclear emergency. But the uncertainty of model parameters, such as source term and meteorological data, may significantly affect the prediction accuracy. Data assimilation (DA) is usually used to improve the model prediction with the measurements. The paper proposed a parameter bias transformation method combined with Lagrangian puff model to perform DA. The method uses the transformation of coordinates to approximate the effect of parameters bias. The uncertainty of four model parameters is considered in the paper: release rate, wind speed, wind direction and plume height. And particle swarm optimization is used for searching the optimal parameters. Twin experiment and Kincaid experiment are used to evaluate the performance of the proposed method. The results show that the proposed method can effectively increase the reliability of model prediction and estimate the parameters. It has the advantage of clear concept and simple calculation. It will be useful for improving the result of atmospheric dispersion model at the early stage of nuclear emergency.

Functional Characterization of the Copper-Transporting P-Type ATPase Gene of Penicillium janthinellum Strain GXCR

Hongmin Lai,Changbin Sun,Huaying Tang,Xianwei Fan,Yili Ma,Youzhi Li 한국미생물학회 2009 The journal of microbiology Vol.47 No.6

Copper (Cu)-transporting P-type ATPase (CTPA) genes have been documented to play an important role in resistance to heavy metals. However, our understanding of roles of CTPA genes of the filamentous fungi was based only on sequence similarity prediction before. In a previous study, we isolated a Penicillum janthinellum strain GXCR of higher tolerance to Cu (200 mM). In this study, we cloned the partial cDNA of CTPA gene, named PcpA, from the strain GXCR. Sequence alignment indicated that the cloned cDNA sequence has the highest identity (94.4%) with a predictive CTPA gene of Aspergillus clavatus. The PcpA- encoded protein, termed PcpA, has classical functional domains of CTPAs, and shows differences from reported CTPAs in some specific sequence motifs and transmembrane regions. Expression of the PcpA was induced by extracellular Cu, cadmium (Cd), and silver (Ag). PcpA RNA interference (RNAi) mutants with a reduced level of PcpA mRNA were more sensitive to Cu, iron, Cd, and Ag than the wild-type (WT) strain GXCR. When grown in the presence of Cu, iron, and Cd, intracellular Cu and iron contents in the PcpA RNAi mutant were significantly (P<0.05) lower than those in the WT; However, intracellular Cd content in the mutant was significantly (P<0.05) higher than that in the WT. Taken together, it can be concluded that the PcpA functions in Cu uptake and homeostasis, iron uptake, and Cd export from the cytosol to the extracytosol.

Helicobacter pylori inhibited cell proliferation in human periodontal ligament fibroblasts through the Cdc25C/CDK1/cyclinB1 signaling cascade

Li, Huanying,Liang, Dongsheng,Hu, Naiming,Dai, Xingzhu,He, Jianing,Zhuang, Hongmin,Zhao, Wanghong Korean Academy of Periodontology 2019 Journal of Periodontal & Implant Science Vol.49 No.3

Purpose: Several studies have shown that the oral cavity is a secondary location for Helicobacter pylori colonization and that H. pylori is associated with the severity of periodontitis. This study investigated whether H. pylori had an effect on the periodontium. We established an invasion model of a standard strain of H. pylori in human periodontal ligament fibroblasts (hPDLFs), and evaluated the effects of H. pylori on cell proliferation and cell cycle progression. Methods: Different concentrations of H. pylori were used to infect hPDLFs, with 6 hours of co-culture. The multiplicity of infection in the low- and high-concentration groups was 10:1 and 100:1, respectively. The Cell Counting Kit-8 method and Ki-67 immunofluorescence were used to detect cell proliferation. Flow cytometry, quantitative real-time polymerase chain reaction, and western blots were used to detect cell cycle progression. In the high-concentration group, the invasion of H. pylori was observed by transmission electron microscopy. Results: It was found that H. pylori invaded the fibroblasts, with cytoplasmic localization. Analyses of cell proliferation and flow cytometry showed that H. pylori inhibited the proliferation of periodontal fibroblasts by causing G2 phase arrest. The inhibition of proliferation and G2 phase arrest were more obvious in the high-concentration group. In the low-concentration group, the G2 phase regulatory factors cyclin dependent kinase 1 (CDK1) and cell division cycle 25C (Cdc25C) were upregulated, while cyclin B1 was inhibited. However, in the high-concentration group, cyclin B1 was upregulated and CDK1 was inhibited. Furthermore, the deactivated states of tyrosine phosphorylation of CDK1 (CDK1-Y15) and serine phosphorylation of Cdc25C (Cdc25C-S216) were upregulated after H. pylori infection. Conclusions: In our model, H. pylori inhibited the proliferation of hPDLFs and exerted an invasive effect, causing G2 phase arrest via the Cdc25C/CDK1/cyclin B1 signaling cascade. Its inhibitory effect on proliferation was stronger in the high-concentration group.

Research on Rapid Detection Method of Dry Matter Content in Raw Milk Based on Mid-infrared Spectrum

Xiaoming Li,Guicheng Huo,Yan Wang,Qingming Kong,Hongmin Sun 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.9

This paper takes raw milk as research object, and using mid-infrared spectrum analysis method rapidly tests dry matter in raw milk through establishing quantitative analysis model. First of all, researchers collect 84 kinds of raw milk which is different areas and varieties in Heilongjiang province, and classify 84 samples into calibration sets and validation sets by standard chemical testing and spectral scanning on raw milk, and calibration sets contain 64 samples, and validation sets contain 20 samples. Then, respectively selecting SPA and GA characteristic wavelength after treating by smoothing denoising method which window size is 11 points, and establishing PLS model and model demonstration. Eventually, researchers draw the conclusion that SPA wavelength selection method which value of m_max is 10 and GA wavelength selection method which value of Evaluat is 10d can effectively improve validation set model precision, but precision of the latter is higher and characteristic wavelength point reduces from 352 to 10, The calibrating determination coefficient R2 of model is 0.8092155, root mean square error (RMSEC ) is 0.1206172. Validation set determination coefficient R2 is 0.8620867, the root mean square error (RMSEC) is 0.0950656, and relative standard deviation RSD<3%. These prove that the method for rapid testing of raw milk components is feasible.

Helicobacter pylori inhibited cell proliferation in human periodontal ligament fibroblasts through the Cdc25C/CDK1/cyclinB1 signaling cascade

Huanying Li,Dongsheng Liang,Naiming Hu,Xingzhu Dai,Jianing He,Hongmin Zhuang,Wanghong Zhao 대한치주과학회 2019 Journal of Periodontal & Implant Science Vol.49 No.3

Purpose: Several studies have shown that the oral cavity is a secondary location for Helicobacter pylori colonization and that H. pylori is associated with the severity of periodontitis. This study investigated whether H. pylori had an effect on the periodontium. We established an invasion model of a standard strain of H. pylori in human periodontal ligament fibroblasts (hPDLFs), and evaluated the effects of H. pylori on cell proliferation and cell cycle progression. Methods: Different concentrations of H. pylori were used to infect hPDLFs, with 6 hours of co-culture. The multiplicity of infection in the low- and high-concentration groups was 10:1 and 100:1, respectively. The Cell Counting Kit-8 method and Ki-67 immunofluorescence were used to detect cell proliferation. Flow cytometry, quantitative real-time polymerase chain reaction, and western blots were used to detect cell cycle progression. In the high-concentration group, the invasion of H. pylori was observed by transmission electron microscopy. Results: It was found that H. pylori invaded the fibroblasts, with cytoplasmic localization. Analyses of cell proliferation and flow cytometry showed that H. pylori inhibited the proliferation of periodontal fibroblasts by causing G2 phase arrest. The inhibition of proliferation and G2 phase arrest were more obvious in the high-concentration group. In the low-concentration group, the G2 phase regulatory factors cyclin dependent kinase 1 (CDK1) and cell division cycle 25C (Cdc25C) were upregulated, while cyclin B1 was inhibited. However, in the high-concentration group, cyclin B1 was upregulated and CDK1 was inhibited. Furthermore, the deactivated states of tyrosine phosphorylation of CDK1 (CDK1-Y15) and serine phosphorylation of Cdc25C (Cdc25C-S216) were upregulated after H. pylori infection. Conclusions: In our model, H. pylori inhibited the proliferation of hPDLFs and exerted an invasive effect, causing G2 phase arrest via the Cdc25C/CDK1/cyclin B1 signaling cascade. Its inhibitory effect on proliferation was stronger in the high-concentration group.

Research on Rapid Detection Method of Protein and Fat in Raw Milk Based on Mid-infrared Spectrum

Xiaoming Li,Guicheng Huo,Yan Wang,Hongmin Sun,Qingming Kong 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.10

In this paper raw milk was taken as the research object, using mid-infrared spectrum analysis method rapidly tests protein and fat content in raw milk through establishing quantitative analysis model. First of all, researchers collect 84 kinds of raw milk which is different areas and varieties in Heilongjiang province, and classify 84 samples into calibration sets and validation sets by standard chemical testing and spectral scanning on protein and fat, and calibration sets contain 64 samples, and validation sets contain 20 samples. Then, denoising by second derivative method which window size is 17 points, and selecting method by comparing SPA and GA characteristic wavelength respectively, and establishing PLS model and model demonstration. Eventually, researchers draw the conclusion that SPA wavelength selection method which value of m_max is 10 and GA wavelength selection method which value of Evaluat is 10d can effectively improve validation set model precision, but precision of the former is higher and characteristic wavelength is least, and its characteristic wavelength point reduces from 220 to 4, R2 of protein model validation set is 0.8010929, RMSEP is 0.0207505, R2 of fat model validation set is 0.91007989, RMSEP is 0.045329, relative standard deviation RSD<3%. These prove that the method for rapid testing of raw milk components is feasible.

Nano/Microstructures of Some DMS Materials Fabricated by High Magnetic Field-hydrothermal Synthesis

YingLi,Mingyuan Zhu,Hongmin Jin,Zhun Wang,Yibing Li,Tao Yang,Huang Jin 한국자기학회 2008 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

Study on tool wear for efficient grooving blisk with disc milling cutter

Qingsi Cheng,Song Wang,Hongmin Xin,Jian Zhang,Changfeng Yao,Minchao Cui,Guangping Li 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.10

Tool wear has an important influence on machining quality, cost, cutting force and heat. Grooving blisk with disc milling cutter is 3-4 times more efficient than plunge milling and side milling, but the tool wear is serious because of the large milling force and high milling temperature during the machining process. In the study, the damage and wear morphology of the tool was observed by focused ion/double beam system; the wear mechanism was studied by energy spectrum analysis; the tool life curve was drawn by the increase of flank wear. The experimental results show that the main wear and damage morphologies of disc milling cutter are chip sticking to the tool, chip accumulation, edge collapse, peeling, crack, plastic deformation and boundary wear; the main wear mechanism are abrasive wear, adhesive wear, oxidation wear and diffusion wear; the wear rate of the left blade is the fastest, followed by the right blade, and the wear rate of the middle blade is the slowest. These results can provide guidance for iimproving tool life, optimizing tool and mproving machining processes.

Associations of Genetic Variations in Mismatch Repair Genes MSH3 and PMS1 with Acute Adverse Events and Survival in Patients with Rectal Cancer Receiving Postoperative Chemoradiotherapy

Jie Yang,Ying Huang,Yanru Feng,Hongmin Li,Ting Feng,Jinna Chen,Luxi Yin,Weihu Wang,Shulian Wang,Yueping Liu,Yongwen Song,Yexiong Li,Jing Jin,Wen Tan,Dongxin Lin 대한암학회 2019 Cancer Research and Treatment Vol.51 No.3

Purpose Mismatch repair (MMR) deficiency plays a critical role in rectal cancer. This study aimed to explore the associations between genetic variations in seven MMR genes and adverse events (AEs) and survival of patients with rectal cancer treated with postoperative chemoradiotherapy (CRT). Materials and Methods Fifty single nucleotide polymorphisms in seven MMR (MLH1, MLH3, MSH2, MSH3, MSH6, PMS1 and PMS2) genes were genotyped by Sequenom MassARRAY method in 365 patients with locally advanced rectal cancer receiving postoperative CRT. The associations between genotypes and AEs were measured by odds ratios and 95% confidence intervals (CIs) by unconditional logistic regression model. The associations between genetic variations and survival were computed by the hazard ratios and 95% CIs by Cox proportional regression model. Results The most common grade ! 2 AEs in those 365 patients, in decreasing order, were diarrhea (44.1%), leukopenia (29.6%), and dermatitis (18.9%). Except 38 cases missing, 61 patients (18.7%) died during the follow-up period. We found MSH3 rs12513549, rs33013, and rs6151627 significantly associated with the risk of grade ! 2 diarrhea. PMS1 rs1233255 had an impact on the occurrence of grade ! 2 dermatitis. Meanwhile, PMS1 rs4920657, rs5743030, and rs5743100 were associated with overall survival time of rectal cancer. Conclusion These results suggest that MSH3 and PMS1 polymorphisms may play important roles in AEs prediction and prognosis of rectal cancer patients receiving postoperative CRT, which can be potential genetic biomarkers for rectal cancer personalized treatment.