Damage detection of shear buildings through structural mass-stiffness distribution

Yabin Liang,Dongsheng Li,Gangbing Song,Chao Zhan 국제구조공학회 2017 Smart Structures and Systems, An International Jou Vol.19 No.1

For structural damage detection of shear buildings, this paper proposes a new concept using structural element mass-stiffness vector (SEMV) based on special mass and stiffness distribution characteristics. A corresponding damage identification method is developed combining the SEMV with the cross-model cross-mode (CMCM) model updating algorithm. For a shear building, a model is assumed at the beginning based on the building\'s distribution characteristics. The model is updated into two models corresponding to the healthy and damaged conditions, respectively, using the CMCM method according to the modal parameters of actual structure identified from the measured acceleration signals. Subsequently, the structural SEMV for each condition can be calculated from the updated model using the corresponding stiffness and mass correction factors, and then is utilized to form a new feature vector in which each element is calculated by dividing one element of SEMV in health condition by the corresponding element of SEMV in damage condition. Thus this vector can be viewed as a damage detection feature for its ability to identify the mass or stiffness variation between the healthy and damaged conditions. Finally, a numerical simulation and the laboratory experimental data from a test-bed structure at the Los Alamos National Laboratory were analyzed to verify the effectiveness and reliability of the proposed method. Both simulated and experimental results show that the proposed approach is able to detect the presence of structural mass and stiffness variation and to quantify the level of such changes.

Helicobacter pylori inhibited cell proliferation in human periodontal ligament fibroblasts through the Cdc25C/CDK1/cyclinB1 signaling cascade

Li, Huanying,Liang, Dongsheng,Hu, Naiming,Dai, Xingzhu,He, Jianing,Zhuang, Hongmin,Zhao, Wanghong Korean Academy of Periodontology 2019 Journal of Periodontal & Implant Science Vol.49 No.3

Purpose: Several studies have shown that the oral cavity is a secondary location for Helicobacter pylori colonization and that H. pylori is associated with the severity of periodontitis. This study investigated whether H. pylori had an effect on the periodontium. We established an invasion model of a standard strain of H. pylori in human periodontal ligament fibroblasts (hPDLFs), and evaluated the effects of H. pylori on cell proliferation and cell cycle progression. Methods: Different concentrations of H. pylori were used to infect hPDLFs, with 6 hours of co-culture. The multiplicity of infection in the low- and high-concentration groups was 10:1 and 100:1, respectively. The Cell Counting Kit-8 method and Ki-67 immunofluorescence were used to detect cell proliferation. Flow cytometry, quantitative real-time polymerase chain reaction, and western blots were used to detect cell cycle progression. In the high-concentration group, the invasion of H. pylori was observed by transmission electron microscopy. Results: It was found that H. pylori invaded the fibroblasts, with cytoplasmic localization. Analyses of cell proliferation and flow cytometry showed that H. pylori inhibited the proliferation of periodontal fibroblasts by causing G2 phase arrest. The inhibition of proliferation and G2 phase arrest were more obvious in the high-concentration group. In the low-concentration group, the G2 phase regulatory factors cyclin dependent kinase 1 (CDK1) and cell division cycle 25C (Cdc25C) were upregulated, while cyclin B1 was inhibited. However, in the high-concentration group, cyclin B1 was upregulated and CDK1 was inhibited. Furthermore, the deactivated states of tyrosine phosphorylation of CDK1 (CDK1-Y15) and serine phosphorylation of Cdc25C (Cdc25C-S216) were upregulated after H. pylori infection. Conclusions: In our model, H. pylori inhibited the proliferation of hPDLFs and exerted an invasive effect, causing G2 phase arrest via the Cdc25C/CDK1/cyclin B1 signaling cascade. Its inhibitory effect on proliferation was stronger in the high-concentration group.

Composite of Indium and Polysorbate 20 as Inhibitor for Zinc Corrosion in Alkaline Solution

Li, Xiaoping,Liang, Man,Zhou, Hebing,Huang, Qiming,Lv, Dongsheng,Li, Weishan Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.5

The combined use of indium and polysorbate 20 (Tween 20) was considered as a new inhibition technique for zinc corrosion. Zn and Zn-In alloy coatings were prepared by electrodeposition and their morphology and composition were characterized by scanning electron microscopy (SEM) and inductively coupled plasma atomic emission spectrometry (ICP-AES). The corrosion inhibition effect of indium and Tween 20 on zinc was investigated by polarization curves and electrochemical impedance spectroscopy (EIS). The corrosion inhibition efficiencies obtained from Tafel and EIS analyses are well in agreement. Zinc corrosion can be inhibited to some extent by the individual use of indium and Tween 20 and higher corrosion inhibition efficiency can be obtained by the combined use of indium and Tween 20.

Helicobacter pylori inhibited cell proliferation in human periodontal ligament fibroblasts through the Cdc25C/CDK1/cyclinB1 signaling cascade

Huanying Li,Dongsheng Liang,Naiming Hu,Xingzhu Dai,Jianing He,Hongmin Zhuang,Wanghong Zhao 대한치주과학회 2019 Journal of Periodontal & Implant Science Vol.49 No.3

Purpose: Several studies have shown that the oral cavity is a secondary location for Helicobacter pylori colonization and that H. pylori is associated with the severity of periodontitis. This study investigated whether H. pylori had an effect on the periodontium. We established an invasion model of a standard strain of H. pylori in human periodontal ligament fibroblasts (hPDLFs), and evaluated the effects of H. pylori on cell proliferation and cell cycle progression. Methods: Different concentrations of H. pylori were used to infect hPDLFs, with 6 hours of co-culture. The multiplicity of infection in the low- and high-concentration groups was 10:1 and 100:1, respectively. The Cell Counting Kit-8 method and Ki-67 immunofluorescence were used to detect cell proliferation. Flow cytometry, quantitative real-time polymerase chain reaction, and western blots were used to detect cell cycle progression. In the high-concentration group, the invasion of H. pylori was observed by transmission electron microscopy. Results: It was found that H. pylori invaded the fibroblasts, with cytoplasmic localization. Analyses of cell proliferation and flow cytometry showed that H. pylori inhibited the proliferation of periodontal fibroblasts by causing G2 phase arrest. The inhibition of proliferation and G2 phase arrest were more obvious in the high-concentration group. In the low-concentration group, the G2 phase regulatory factors cyclin dependent kinase 1 (CDK1) and cell division cycle 25C (Cdc25C) were upregulated, while cyclin B1 was inhibited. However, in the high-concentration group, cyclin B1 was upregulated and CDK1 was inhibited. Furthermore, the deactivated states of tyrosine phosphorylation of CDK1 (CDK1-Y15) and serine phosphorylation of Cdc25C (Cdc25C-S216) were upregulated after H. pylori infection. Conclusions: In our model, H. pylori inhibited the proliferation of hPDLFs and exerted an invasive effect, causing G2 phase arrest via the Cdc25C/CDK1/cyclin B1 signaling cascade. Its inhibitory effect on proliferation was stronger in the high-concentration group.

Microstructure Change of Nanosilica-Cement Composites Partially Exposed to Sulfate Attack

Qian Huang,Liang Zhao,Chenggong Zhao,Dongsheng Liu,Chaoqiang Wang 한국콘크리트학회 2020 International Journal of Concrete Structures and M Vol.14 No.3

The deterioration of cement composites containing nanosilica partially exposed to sulfate attack was studied, and the microstructure change of the composites was analysed by a scanning electron microscope (SEM), energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD). The results showed that nanosilica-cement composites had better sulfate resistance compared to plain cement composite under partial exposure to sulfate attack, and their sulfate resistance increased as the nanosilica content increased (in the range of 0 to 5 wt% replacing cement by weight). The main sulfate products were gypsum and ettringite within the surface and inner parts, respectively in both the immersed and evaporation portions of the nanosilica-cement composites, which was consistent with the plain cement composite. Thus, the incorporation of nanosilica did not change the distribution characteristics of the sulfate products within the composites partially exposed to sulfate attack. However, the addition of nanosilica reduced the amount of sulfate products in both the immersed and evaporation portions, and their amount decreased with the increase of nanosilica content. The evaporation portions of the composites suffered chemical sulfate attack rather than sulfate salt crystallization. Nanosilica-cement composites could be applied in real partial exposure environments containing sulfate ions.

Composite of Indium and Polysorbate 20 as Inhibitor for Zinc Corrosion in Alkaline Solution

Xiaoping Li,Man Liang,Hebing Zhou,Qiming Huang,Dongsheng Lv,Weishan Li 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.5

The combined use of indium and polysorbate 20 (Tween 20) was considered as a new inhibition technique for zinc corrosion. Zn and Zn-In alloy coatings were prepared by electrodeposition and their morphology and composition were characterized by scanning electron microscopy (SEM) and inductively coupled plasma atomic emission spectrometry (ICP-AES). The corrosion inhibition effect of indium and Tween 20 on zinc was investigated by polarization curves and electrochemical impedance spectroscopy (EIS). The corrosion inhibition efficiencies obtained from Tafel and EIS analyses are well in agreement. Zinc corrosion can be inhibited to some extent by the individual use of indium and Tween 20 and higher corrosion inhibition efficiency can be obtained by the combined use of indium and Tween 20.