SIMPLE SYNTHESIS OF UNIFORM α-Fe2O3 MICROSPHERES WITH EXCELLENT PHOTOCATALYTIC PERFORMANCE USING POLY (N-VINYL-2-PYRROLIDONE)

TING-TING ZHANG,TING-TING GUO,ZHI-PING WU,SI-WEI YANG,ZHOU-FENG WANG,FANG LIAO 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.5

In this paper, a new simple approach has been developed for the preparation of α-Fe2O3 microspheres by a facile hydrothermal method using PVP as a surfactant. Uniform α-Fe2O3 microspheres could be routinely synthesized through solvothermal approach by controlling the PVP/FeCl3⋅6H2O ratio. The as-obtained α-Fe2O3 microspheres exhibit high efficiency on the decolorization of RhB aqueous solution in the presence of H2O2 at room temperature.

Differential microRNA Expression by Solexa Sequencing in the Sera of Ovarian Cancer Patients

Ji, Ting,Zheng, Zhi-Guo,Wang, Feng-Mei,Xu, Li-Jian,Li, Lu-Feng,Cheng, Qi-Hui,Guo, Jiang-Feng,Ding, Xian-Feng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

MicroRNAs are a class of small noncoding RNA which play important regulatory roles in a variety of cancers. MiRNA-specific expression profiles have been reported for several pathological conditions. In this study, we combined large scale parallel Solexa sequencing to identify 11 up-regulated miRNAs and 19 down-regulated miRNAs with computational techniques in the sera of ovarian cancer patients while using healthy serum as the control. Among the above, four miRNAs (miR-22, miR-93, miR-106b, miR-451) were validated by quantitative RT-PCR and found to be significantly aberrantly expressed in the serum of ovarian cancer patients (P<0.05). There were no significant differences between samples from cancer stage I/II and III/IV. However, the levels of miR-106b (p=0.003) and miR-451 (p=0.007) were significantly different in those patients under and over 51 yearsof age. MiR-451 and miR-93 were also specific when analyzed with reference to different levels of CA125. This study shows that Solexa sequencing provides a promising method for cancer-related miRNA profiling, and selectively expressed miRNAs could be used as potential serum-based biomarkers for ovarian cancer diagnosis.

Synthesis, Characterization, and Micellization of pH-Responsive Poly(4-vinylpyridine)-block-Poly(methacrylic acid) Four-Armed Star-Shaped Block Copolymers

Feng Xu,Shu-Zhen Zheng,Yan-Ling Luo,Ting-Ting Chen 한국고분자학회 2013 Macromolecular Research Vol.21 No.9

pH-sensitive poly(4-vinylpyridine)-block-poly(methacrylic acid) (P4VP-b-PMAA) four-armed starshaped block copolymers were synthesized by two-step atom transfer radical polymerization (ATRP), followed by hydrolysis of P4VP-b-poly(tert-butyl methacrylate) (P4VP-b-PtBMA). The chemical structure and molecular weight of the as-synthesized block copolymers were characterized by Fourier transform infrared spectrometry (FTIR), nuclear magnetic resonance (1H and 13C NMR), and gel permeation chromatography (GPC) determinations. The solution behavior was investigated by surface tension technique, ultraviolet visible (UV-vis) transmittance,transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta potentials measurements. The experimental results indicated that the copolymers can spontaneously assemble into spherical-shaped core-shell micelle aggregates, with a critical micelle concentration (CMC) about 200 mg L-1, hydrodynamic diameters from 90to 210 nm, depending on the environmental pH values and compositional ratios. The transmittance measurements revealed that the block copolymers produce evident phase transition in aqueous solution at pH from 6.5 to 7.0. Zeta potential data revealed high micelle stability. The as-synthesized block copolymers are anticipated to find their applications in the realms of specific drug release, metal loading and heterogeneous catalysis.

Synthesis and Micellization of Thermosensitive PNIPAAm-b-PLA Amphiphilic Block Copolymers Based on a Bifunctional Initiator

Feng Xu,Yan-Ling Luo,Ting-Ting Yan 한국고분자학회 2011 Macromolecular Research Vol.19 No.12

The thermo-sensitive amphiphilic block copolymer poly(N-isopropylacrylamide)-block-poly(D,L-lactide)(PNIPAAm-b-PLA) was synthesized using a simple free radical copolymerization route based on a bifunctional initiator, 2,2-azobis(2-methylpropion amidine) dihydrochloride followed by the ring-opening polymerization of D,L-actide in the presence of a Sn(Oct)_2 catalyst. The chemical structure of the PNIPAAm-b-PLA copolymers was verified using Fourier transform-infrared spectrophotometry and nuclear magnetic resonance, and the molecular weight and polydispersity index were examined using gel permeation chromatography. The amphiphilic PNIPAAm-b-PLA block copolymers could self-assemble into spherically shaped micelles in an aqueous solution with a transmission electron microscopy diameter range of 40-56 nm and a dynamic laser scattering hydrodynamic diameter of 90-200 nm. This behavior depends on the environmental temperature, the hydrophobic interactions among PNIPAAm molecular chains, the intermolecular hydrogen bonding between the PNIPAAm chains and water molecules, and the intramolecular hydrogen bonding between the -CONH_2 groups. The copolymers held a critical micellization concentration of 4.93-7.21 mg·L^-1 and a low critical solution temperature of 31.15-32.62 ℃ being more or less affected by their compositions, PLA or PNIPAAm block length, and polymerization temperature. The as-prepared PNIPAAm-b-PLA block polymers are anticipated to be applied as candidate drug release carriers.

Effect of Potassium on Flavor Quality and LOX Activity of Cucumber in Solar-Greenhouse

Feng-ting Wang,Jin-liang Liu,Kun-fan Xu,Xi-zhen Ai 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Fabrication of polyacrylic acid-based composite binders with strong binding forces on copper foils for silicon anodes in lithium-ion batteries

Ting-Ting Su,Wen-Feng Ren,Jiang-Meng Yuan,Ke Wang,Bing-Yu Chi,Run-Cang Sun 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.109 No.-

The commercial application of silicon (Si) anodes with high theoretical capacity is hampered by the poorcyclic stability because the huge volume change of Si during discharge/charge processes results in thepulverization of electrode materials and the electric contact loss of electrode materials with copper(Cu) foils. Binders play an important role for adhering active materials and conductive additives togetheronto Cu foils. Herein, we design and develop a three-dimensional networked composite binder (PAA-co-SN) via an amidation reaction between polyacrylic acid (PAA) and thiourea (SN). The granular anchors(CuxS) are constructed at the interface between PAA-co-SN binders and Cu foils, resulting from the reactionof S and Cu during the drying processes of Si electrodes. When used as binders for Si nanoparticles,they exhibit stable cyclic performance (1580 mAh g1 after 500 cycles). The anchoring mechanismbetween composite binders and Cu foils provides a strategy for the improvement of electrochemicalproperties of Si-based anodes for lithium-ion batteries.

The impact of lymph node dissection on survival in patients with clinical early-stage ovarian cancer

Ting Deng,Qidan Huang,Ting Wan,Xiaoling Luo,Yanling Feng,He Huang,Jihong Liu 대한부인종양학회 2021 Journal of Gynecologic Oncology Vol.32 No.3

Objective: To estimate the impact of lymph node dissection on survival in patients withapparent early-stage epithelial ovarian cancer (EOC). Methods: We conducted a retrospective review of patients with clinical stage I–II EOC. Allpatients underwent primary surgery at Sun Yat-sen University Cancer Center between January2003 and December 2015. Demographic features and clinicopathological information as wellas perioperative adverse events were investigated, and survival analyses were performed. Results: A total of 400 ovarian cancer patients were enrolled, and patients were divided into2 groups: 81 patients did not undergo lymph node resection (group A), and 319 patientsunderwent lymph node dissection (group B). In group B, the median number of removednodes per patient was 25 (21 pelvic and 4 para-aortic nodes). In groups A and B, respectively,the 5-year progression-free survival (PFS) rates were 83.3% and 82.1% (p=0.305), and the5-year overall survival (OS) rates were 93.1% and 90.9% (p=0.645). The recurrence rate inthe retroperitoneal lymph nodes was not associated with lymph node dissection (p=0.121). The median operating time was markedly longer in group B than in group A (220 minutesvs. 155 minutes, p<0.001), and group B had a significantly higher incidence of lymph cysts atdischarge (32.9% vs. 0.0%, p<0.001). Conclusion: In patients with early-stage ovarian cancer, lymph node dissection was notassociated with a gain in OS or PFS and was associated with an increased incidence ofperioperative adverse events.

Flavihumibacter profundi sp. nov., isolated from eutrophic freshwater sediment

Ting-Ting Ren,Chunzhi Jin,Feng-Jie Jin,Taihua Li,김창진,오희목,이형관,Long Jin 한국미생물학회 2018 The journal of microbiology Vol.56 No.7

A Gram-stain-positive, aerobic, non-motile, non-spore-forming, and rod-shaped bacterium, designated strain CHu64- 6-1T, was isolated from a 67-cm-long sediment core collected from the Daechung Reservoir at a water depth of 17-m in Daejeon, Republic of Korea. Comparative 16S rRNA gene sequence studies placed the new isolate in the class Sphingobacteriia, and the isolate is notably most closely related to Flavihumibacter sediminis CJ663T (98.1% similarity), Flavihumibacter solisilvae 3-3T (97.8%), Flavihumibacter petaseus T41T (97.5%), Flavihumibacter cheonanensis WS16T (97.4%), and Flavihumibacter stibioxidans YS-17T (97.2%). The cells of strain CHu64-6-1T formed yellow colonies on R2A agar and contained MK-7 as the only menaquinone, phosphatidylethanolamine, an unidentified phospholipid, and two unidentified aminolipids as the major polar lipids, and C15:0 iso, C17:0 iso 3-OH, C15:1 iso G, and C16:1 ω5c as the major fatty acids (> 5%). The DNA G + C content of the genome was determined to be 46.5 mol%. The DNA-DNA hybridization values of strain CHu64-6-1T with F. sediminis CJ663T, F. solisilvae 3-3T, F. petaseus T41T, F. cheonanensis WS16T, and F. stibioxidans YS-17T were 12.4–33.2%. Based on the combined genotypic and phenotypic data, we propose that strain CHu64-6-1T represents a novel species of the genus Flavihumibacter, for which the name Flavihumibacter profundi sp. nov. is proposed. The type strain is CHu64-6-1T (= KCTC 62290T = CCTCC AB 2018060T).

A Novel All-trans Retinoid Acid Derivative N-(3-trifluoromethyl-phenyl)-Retinamide Inhibits Lung Adenocarcinoma A549 Cell Migration through Down-regulating Expression of Myosin Light Chain Kinase

Fan, Ting-Ting,Cheng, Ying,Wang, Yin-Feng,Gui, Shu-Yu,Chen, Fei-Hu,Zhou, Qing,Wang, Yuan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Aim: To observe the effects of a novel all-trans retinoid acid (ATRA) derivative, N-(3-trifluoromethyl-phenyl)-retinamide (ATPR), on lung adenocarcinoma A549 cells and to explore the potential mechanism of ATPR inhibiting of A549 cell migration. Materials and Methods: The cytotoxicity of ATRA and ATPR on A549 cells was assessed using MTT assay. Wound healing assays were used to analyze the influences of ATRA, ATPR, ML-7 (a highly selective inhibitor of myosin light chain kinase (MLCK)), PMA (an activator of MAPKs) and PD98059 (a selective inhibitor of ERK1/2) on the migration of A549 cells. Expression of MLCK and phosphorylation of myosin light chain (MLC) were assessed by Western blotting. Results: ATRA and ATPR inhibited the proliferation of A549 cells in a dose- and time-dependent manner, and the effect of ATPR was much more remarkable compared with ATRA. Relative migration rate and migration distance of A549 cells both decreased significantly after treatment with ATPR or ML-7. The effect on cell migration of PD98059 combining ATPR treatment was more notable than that of ATPR alone. Moreover, compared with control groups, the expression levels of MLCK and phosphorylated MLC in A549 cells were both clearly reduced in ATRA and ATPR groups. Conclusions: ATPR could suppress the migration and invasion of A549 cells, and the mechanism might be concerned with down-regulating the expression of MLCK in the ERK-MAPK signaling pathway, pointing to therapeutic prospects in lung cancer.

Kinetics of a Cloned Special Ginsenosidase Hydrolyzing 3-O-Glucoside of Multi-Protopanaxadiol-Type Ginsenosides, Named Ginsenosidase Type 3

( Xue Feng Jin ),( Hong Shan Yu ),( Dong Ming Wang ),( Ting Qiang Liu ),( Chun Ying Liu ),( Dong Shan An ),( Wan Taek Im ),( Song Gun Kim ),( Feng Xie Jin ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.3

In this paper, the kinetics of a cloned special glucosidase, named ginsenosidase type III hydrolyzing 3-O-glucoside of multi-protopanaxadiol (PPD)-type ginsenosides, were investigated. The gene (bgpA) encoding this enzyme was cloned from a Terrabacter ginsenosidimutans strain and then expressed in E. coli cells. Ginsenosidase type III was able to hydrolyze 3-O-glucoside of multi-PPD-type ginsenosides. For instance, it was able to hydrolyze the 3- O-β-D-(1→2)-glucopyranosyl of Rb1 to gypenoside XVII, and then to further hydrolyze the 3-O-β-D-glucopyranosyl of gypenoside XVII to gypenoside LXXV. Similarly, the enzyme could hydrolyze the glucopyranosyls linked to the 3-O- position of Rb2, Rc, Rd, Rb3, and Rg3. With a larger enzyme reaction Km value, there was a slower enzyme reaction speed; and the larger the enzyme reaction Vmax value, the faster the enzyme reaction speed was. The Km values from small to large were 3.85 mM for Rc, 4.08 mM for Rb1, 8.85 mM for Rb3, 9.09 mM for Rb2, 9.70 mM for Rg3(S), 11.4 mM for Rd and 12.9 mM for F2; and Vmax value from large to small was 23.2 mM/h for Rc, 16.6 mM/h for Rb1, 14.6 mM/h for Rb3, 14.3 mM/h for Rb2, 1.81mM/h for Rg3(S), 1.40 mM/h for Rd, and 0.41 mM/h for F2. According to the Vmax and Km values of the ginsenosidase type III, the hydrolysis speed of these substrates by the enzyme was Rc>Rb1>Rb3>Rb2>Rg3(S)>Rd>F2 in order.