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        Functional Characterization of the Copper-Transporting P-Type ATPase Gene of Penicillium janthinellum Strain GXCR

        Hongmin Lai,Changbin Sun,Huaying Tang,Xianwei Fan,Yili Ma,Youzhi Li 한국미생물학회 2009 The journal of microbiology Vol.47 No.6

        Copper (Cu)-transporting P-type ATPase (CTPA) genes have been documented to play an important role in resistance to heavy metals. However, our understanding of roles of CTPA genes of the filamentous fungi was based only on sequence similarity prediction before. In a previous study, we isolated a Penicillum janthinellum strain GXCR of higher tolerance to Cu (200 mM). In this study, we cloned the partial cDNA of CTPA gene, named PcpA, from the strain GXCR. Sequence alignment indicated that the cloned cDNA sequence has the highest identity (94.4%) with a predictive CTPA gene of Aspergillus clavatus. The PcpA- encoded protein, termed PcpA, has classical functional domains of CTPAs, and shows differences from reported CTPAs in some specific sequence motifs and transmembrane regions. Expression of the PcpA was induced by extracellular Cu, cadmium (Cd), and silver (Ag). PcpA RNA interference (RNAi) mutants with a reduced level of PcpA mRNA were more sensitive to Cu, iron, Cd, and Ag than the wild-type (WT) strain GXCR. When grown in the presence of Cu, iron, and Cd, intracellular Cu and iron contents in the PcpA RNAi mutant were significantly (P<0.05) lower than those in the WT; However, intracellular Cd content in the mutant was significantly (P<0.05) higher than that in the WT. Taken together, it can be concluded that the PcpA functions in Cu uptake and homeostasis, iron uptake, and Cd export from the cytosol to the extracytosol.

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        Capillarity-Driven Self-Assembly of Silver Nanowires-Coated Fibers for Flexible and Stretchable Conductor

        Yi Li,Jun Chen,Xiao Han,Yinghui Li,Ziqiang Zhang,Yanwen Ma 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.12

        The rapid development of smart textiles requires the large-scale fabrication of conductive fibers. In this study, we develop a simple, scalable and low-cost capillary-driven self-assembly method to prepare conductive fibers with uniform morphology, high conductivity and good mechanical strength. Fiber-shaped flexible and stretchable conductors are obtained by coating highly conductive and flexible silver nanowires (Ag NWs) on the surfaces of yarn and PDMS fibers through evaporation-induced flow and capillary-driven self-assembly, which is proven by the in situ optical microscopic observation. The density of Ag NWs and linear resistance of the conductive fibers could be regulated by tuning the assembly cycles. A linear resistance of 1.4Ω/cm could be achieved for the Ag NWs-coated nylon, which increases only 8% after 200 bending cycle, demonstrating high flexibility and mechanical stability. The flexible and stretchable conductive fibers have great potential for the application in wearable devices.

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        Cascade hydrogenation of n-C16 to produce jet fuel over tandem catalysts of modified ZSM-22

        Yi Li,Junhao Sun,Jie Wei,Chao Mu,Yujun Zhao,Shengping Wang,Xinbin Ma 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.111 No.-

        ZSM-22 with one-dimensional straight channels and suitable acid properties is widely applied in theselective hydrocracking of long-chain alkanes to produce middle distillate. In this work, the tandem catalystswith two kinds of modified ZSM-22 were employed in series to optimize the distribution of theproduct in the hydrotreatment of n-C16 to produce jet fuel. The yield of linear alkanes with high carbonnumbers was enhanced over the upstream catalyst bed of Pt/L-HZSM-22 with shielded external acid siteswhile the isomerization with less further cracking occurred over the downstream catalyst bed of Pt/Me-HZSM-22 with introduced mesopores. The tandem catalysts can combine the ability of Pt/L-HZSM-22 toproduce heavy n-alkanes and the stronger isomerization performance of Pt/Me-HZSM-22. The highestyield of jet fuel (23 wt.%) can be obtained over the tandem catalysts instead of any individual catalyst. The strategy of the tandem zeolite has been initially verified and proposed to broaden the investigationperspectives of the oil refinery.

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