The Methylenetetrahydrofolate Reductase C677T Polymorphism Influences Risk of Esophageal Cancer in Chinese

Qu, Hong-Hong,Cui, Li-Hong,Wang, Ke,Wang, Peng,Song, Chun-Hua,Wang, Kai-Juan,Zhang, Jian-Ying,Dai, Li-Ping Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Methylenetetrahydrofolate reductase (MTHFR) plays a central role in folate metabolism. This study with 381 esophageal cancer patients and 432 healthy controls was conducted to examine the association of MTHFR C677T and A1298C polymorphisms with susceptibility to esophageal cancer (EC) in a Chinese population. Compared with the CC genotype of MTHFR C677T, subjects carrying homozygote TT and variant genotypes (CT+TT) demonstrated reduced risk of EC with adjusted ORs (95% CI) of 0.44 (0.28-0.71) and 0.57 (0.37-0.88), respectively. However, no association was found between the MTHFR A1298C polymorphism and the risk of EC. Comparing to haplotype CA, haplotypes TA and TC could reduce the susceptibility to EC with adjusted ORs (95% CI) of 0.61(0.47-0.79) and 0.06 (0.01-0.43), respectively. In conclusion, the present study suggested that the MTHFR C677T polymorphism can markedly influence the risk of EC in Chinese.

Packing trees into complete k-partite graph

Yanling Peng,Hong Wang 대한수학회 2022 대한수학회보 Vol.59 No.2

In this work, we confirm a weak version of a conjecture proposed by Hong Wang. The ideal of the work comes from the tree packing conjecture made by Gy\'arf\'as and Lehel. Bollob\'as confirms the tree packing conjecture for many small tree, who showed that one can pack $T_1,T_2,\ldots,T_{n/\sqrt{2}}$ into $K_n$ and that a better bound would follow from a famous conjecture of Erd\H{o}s. In a similar direction, Hobbs, Bourgeois and Kasiraj made the following conjecture: Any sequence of trees $T_1,T_2,\ldots,T_n$, with $T_i$ having order $i$, can be packed into $K_{n-1,\lceil n/2\rceil}$. Further Hobbs, Bourgeois and Kasiraj \cite{3} proved that any two trees can be packed into a complete bipartite graph $K_{n-1,\lceil n/2\rceil}$. Motivated by the result, Hong Wang propose the conjecture: For each $k$-partite tree $T(\mathbb{X})$ of order $n$, there is a restrained packing of two copies of $T(\mathbb{X})$ into a complete $k$-partite graph $B_{n+m}(\mathbb{Y})$, where $m=\lfloor\frac{k}{2}\rfloor$. Hong Wong \cite{4} confirmed this conjecture for $k=2$. In this paper, we prove a weak version of this conjecture.

Synthesis, Structures and Properties of Three Metal-organic Frameworks Based on 3-(4-((1H-imidazol-1-yl)methyl)phenyl)acrylic Acid

Peng Liang,Tian-Tian Ren,Wei-Man Tian,Wen-Jia Xu,Gang-Hong Pan,Xian-Hong Yin 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.1

Three new transition metal complexes based on Ozagrel [Cu(Ozagrel)]n (1), [Zn(Ozagrel)(Cl)]n (2), {[Mn2- (Ozagrel)(1,4-ndc)2]·(H2O)}n (3), (Ozagrel = 3-(4-((1H-imidazol-1-yl)methyl)phenyl)acrylic acid; 1,4-ndc = 1,4-Naphthalenedicarboxylic acid) have been hydrothermally synthesized and characterized by elemental analyse, IR, TG, PXRD, electrochemical analysis and single crystal X-ray diffraction. X-ray structure analysis reveals that 1 and 3 are 3D coordination polymers, while complex 2 is a two-dimensional network polymer, the 2D layers are further packed into 3D supramolecular architectures that are connected through hydrogen bonds. The electrochemistry of 1-3 was studied by cyclic voltammetry in methanol and water using a glassy carbon working electrode. Also, thermal decomposition process and powder X-ray diffraction of complexes were investigated.

Structure Analysis of 16S rDNA Sequences from Strains of Acidithiobacillus ferrooxidans

Peng, Hong,Yang, Yu,Li, Xuan,Qiu, Guanzhou,Liu, Xueduan,Huang, Jufang,Hu, Yuehua Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.2

Four strains of Acidithiobacillus ferrooxidans with different iron oxidation capacity were isolated from different mine drainage stations. The 16S rRNA gene of these strains were cloned and sequenced. Based on our sequences analysis on the four strain and the data on the other strains deposited in Genbank, all A. ferrooxidans may be classified into three phylogenetic groups. The analysis data showed that nucleotide variables (signature sites) were detected in 21 positions, and most of them were found in the first 800bp from 5' terminal except position 970 and 1375. Interestingly, the first 13 signature sites were located in two main regions:the first region (position 175-234) located in V2 while the second region (position 390-439) were detected in constant region between V2 and V3. Furthermore, the secondary structure and minimal free energy were determined in two regions among strains of three groups. These results may be useful in characterizing the microevolutionary mechanisms of species formation and monitoring in biohydrometallurgical application.

Ginsenoside Ro, an oleanolic saponin of Panax ginseng, exerts antiinflammatory effect by direct inhibiting toll like receptor 4 signaling pathway

Hong-Lin Xu,Guang-Hong Chen,Yu-Ting Wu,Ling-Peng Xie,Zhang-Bin Tan,Bin Liu,Hui-Jie Fan,Hong-Mei Chen,Gui-Qiong Huang,Min Liu,Ying-Chun Zhou 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.1

Background: Panax ginseng Meyer (P. ginseng), a herb distributed in Korea, China and Japan, exerts benefits on diverse inflammatory conditions. However, the underlying mechanism and active ingredients remains largely unclear. Herein, we aimed to explore the active ingredients of P. ginseng against inflammation and elucidate underlying mechanisms. Methods: Inflammation model was constructed by lipopolysaccharide (LPS) in C57BL/6 mice and RAW264.7 macrophages. Molecular docking, molecular dynamics, surface plasmon resonance imaging (SPRi) and immunofluorescence were utilized to predict active component. Results: P. ginseng significantly inhibited LPS-induced lung injury and the expression of proinflammatory factors, including TNF-a, IL-6 and IL-1b. Additionally, P. ginseng blocked fluorescence-labeled LPS (LPS488) binding to the membranes of RAW264.7 macrophages, the phosphorylation of nuclear factor-kB (NF-kB) and mitogen-activated protein kinases (MAPKs). Furthermore, molecular docking demonstrated that ginsenoside Ro (GRo) docked into the LPS binding site of toll like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD2) complex. Molecular dynamic simulations showed that the MD2-GRo binding conformation was stable. SPRi demonstrated an excellent interaction between TLR4/MD2 complex and GRo (KD value of 1.16 × 10<SUP>-9</SUP> M). GRo significantly inhibited LPS488 binding to cell membranes. Further studies showed that GRo markedly suppressed LPS-triggered lung injury, the transcription and secretion levels of TNF-α, IL-6 and IL-1β. Moreover, the phosphorylation of NF-kB and MAPKs as well as the p65 subunit nuclear translocation were inhibited by GRo dose-dependently. Conclusion: Our results suggest that GRo exerts anti-inflammation actions by direct inhibition of TLR4 signaling pathway.

Experiment and Analysis Verification of Shearing Fracture Characteristic of Tapered Double Cantilever Beam with Aluminum Foam

Hong Peng Sun,조재웅 한국정밀공학회 2015 International Journal of Precision Engineering and Vol.16 No.7

In this study, mode II(sliding) adhesive strengths of tapered double-cantilever beam (TDCB) specimens of aluminum foams bonded with adhesives were evaluated by finite element analysis. Models were fabricated for testing, and their length and thickness were 200 mm and 25 mm, respectively, while the angles between the adhesive layers and the horizontal direction for models (a), (b), and (c) were 8o, 10o, and 12o, respectively. The joint adhesive method was applied to the models that were made of Al-SAF40, aluminum foam. To generate the crack in shear direction, one end of the loaded block was restrained with a frictionless support while the other end of the loaded block was set to be displaced at the rate of 10 mm/min in the adhesive layer. The analysis and comparison result between the 3 different models revealed that the larger the tilt angle θ of sliding mode was, the larger the maximum load became, while the time to reach the maximum load and the time taken until the load disappeared were shorter as the value increased in the displacement-force reaction curve. This study can be applied to real structures with tapered contact surfaces by analyzing fracture behaviors and characteristics of composite materials such as aluminum foams bonded with adhesives.

Association of Reduced Immunohistochemical Expression of E-cadherin with a Poor Ovarian Cancer Prognosis - Results of a Meta-analysis

Peng, Hong-Ling,He, Lei,Zhao, Xia Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Purpose: E-cadherin is a transmemberane protein which is responsible for adhesion of endothelial cells. The aim of our study was to assess existing evidence of associations between reduced expression of E-cadherin and prognosis of ovarian cancer with a discussion of potential approaches to exploiting any prognostic value for improved clinical management. Methods: We conducted a meta-analysis of 9 studies (n=915 patients) focusing on the correlation of reduced expression of E-cadherin with overall survival. Data were synthesized with random or fixed effect hazard ratios. Results: The studies were categorized by author/year, number of patients, FIGO stage, histology, cutoff value for E-cadherin positivity, and methods of hazard rations (HR) estimation, HR and its 95% confidence interval (CI). Combined hazard ratios suggested that reduced expression of E-cadherin positivity was associated with poor overall survival (OS), HR= 2.10, 95% CI:1.13-3.06. Conclusion: The overall survival of the E-cadherin negative group with ovarian cancer was significant poorer than the E-cadherin positive group. Upregulation of E-cadherin is an attractive therapeutic approach that could exert significant effects on clinical outcome of ovarian cancer.

Role of miR-511 in the Regulation of OATP1B1 Expression by Free Fatty Acid

Peng, Jin Fu,Liu, Li,Guo, Cheng Xian,Liu, Shi Kun,Chen, Xiao Ping,Huang, Li Hua,Xiang, Hong,Huang, Zhi Jun,Yuan, Hong,Yang, Guo Ping The Korean Society of Applied Pharmacology 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.5

MicroRNAs (miRNAs) are a family of non-coding RNA that are able to adjust the expression of many proteins, including ATP-binding cassette transporter and organic cation transporter. We sought to evaluate the effect of miR-511 on the regulation of OATP1B1 expression by free fatty acids. When using free fatty acids to stimulate Chang liver cells, we found that the expression of miR-511 increased significantly while the expression of OATP1B1 decreased. We also proved that SLCO1B1 is the target gene of miR-511 with a bioinformatics analysis and using the dual luciferase reporter assay. Furthermore, the expressions of SLCO1B1 and OATP1B1 decreased if transfecting Chang liver cells with miR-511, but did not increase when transfecting the inhibitors of miR-511 into steatosis cells. Our study indicates that miR-511 may play an important role in the regulation of OATP1B1 expression by free fatty acids.

Incorporating Energy-Aware Mechanism into Workflow Scheduling Policy in Heterogeneous Distributed Systems

Hong He,Peng Xiao 보안공학연구지원센터 2014 International Journal of Grid and Distributed Comp Vol.7 No.6

In the past decades, plenty scheduling policies have been proposed for improving the execution performance of workflow applications. However, few of them have addressed the issue of energy conservation. In this paper, a novel scheduling metric, namely Minimal Energy Consumption Path, is introduced to reducing the energy consumption when scheduling large-scale workflows. The proposed scheduling metric can be incorporated into current schedulers so as to enhance their capability of energy conservation as well as maintain their scheduling performance. A lot of experiments are performed by using different scheduler and experimental settings to evaluate the proposed scheduling metric and the results indicate it is effective for reducing the storage-related energy consumption, especially when the target workflows are data-intensive.

Realgar transforming solution suppresses angiogenesis and tumor growth by inhibiting VEGF receptor 2 signaling in vein endothelial cells

Peng Song,Yang Hai,Xin Wang,Longhe Zhao,Baoqiang Chen,Peng Cui,Qin-Jian Xie,Lan Yu,Yang Li,Zhengrong Wu,Hong Yu Li 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.4

Realgar (As4S4), as an arsenic sulfide mineraldrug, has a good therapeutic reputation for anticancer inTraditional Chinese Medicine, and has recently beenreported to inhibit angiogenesis in tumor growth. However,considering the poor solubility and low bioavailability ofrealgar, large dose of realgar and long period of treatmentare necessary for achieving the effective blood medicineconcentration. In present study, we resolved the crucialproblem of poor solubility of realgar by using intrinsicbiotransformation in microorganism, and investigatedunderlying mechanisms of realgar transforming solution(RTS) for antiangiogenesis. Our results demonstrated thatRTS had a strong activity to inhibit HUVECs proliferation,migration, invasion, and tube formation. Moreover, RTSinhibited VEGF/bFGF-induced phosphorylation ofVEGFR2 and the downstream protein kinases includingERK, FAK, and Src. In vivo zebrafish and chickenchorioallantoic membrane model experiments showed thatRTS remarkably blocked angiogenesis. Finally, comparedwith the control, administration of 2.50 mg/kg RTSreached more than 50% inhibition against H22 tumorallografts in KM mice, but caused few toxic effects in thehost. The antiangiogenic effect was indicated by CD31immunohistochemical staining and alginate-encapsulatedtumor cell assay. In summary, our findings suggest thatRTS inhibits angiogenesis and may be a potential drugcandidate in anticancer therapy.