http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
PC-766B' and PC-766B, 16-Membered Macrolide Angiogenesis Inhibitors Produced by Nocardia sp. RK97-56
KO, HACK-RYONG,KAKEYA, HIDEAKI,YOSHIDA, ARIKA,ONOSE, RIE,UEKI, MASASHI,MUROI, MAKOTO,TAKATSUKI, AKIRA,MATSUZAKI, HIROSHI,OSADA, HIROYUKI 한국미생물 · 생명공학회 2002 Journal of microbiology and biotechnology Vol.12 No.5
Angiogenesis is an essential event in a variety of physiological and pathological processes. Therefore, effective inhibition of event is a promising strategy for treating angiogenesis-related diseases, including cancer. The current study investigated two unique bafilomycin-type macrolide inhibitors of angiogenesis, PC-766B' (1) and PC-766B (2). The strain RK97-56 which produced the inhibitors was identified as Nocardia sp. by chemotaxonomic analyses, and the purification of the inhibitors was guided by their anti-angiogenic actives. PC-766B' (1) and PC-766B (2) exhibited potent inhibitory activities towards endothelial cell migration stimulated by the vascular endothelial growth factor(VEGF).
Streptomyces sp. ZF-10이 생산하는 세포주기 저해제
하상철,홍순덕,Osada, Hiroyuki 한국산업미생물학회 1994 한국미생물·생명공학회지 Vol.22 No.5
Genistein은 우리의 톡특한 탐색계를 통한 G_1과 G_2기에서 세포주기 저해제로서 발견되었다. 이 system은 mouse 유방암 세포 주기의 progression에서 미생물 대사산물이 flow cytometry에 의해 모니터되는 system이다. Zaire 토양에서 분리한 Streptomyces ZF-10으로부터 G_l, G_2기의 특이적인 세포주기 저해제인 항생물질 HS-3를 용매추출, silica gel column chromatography, HPLC를 통하여 분리정제하였다. 그래서 UV, ^1H-NMR spectrum 분석을 통해 HS-3는 genistein으로 동정되었다. Genistein, a inhibitor of the progression of G_1 and G_2 phases of the mammalian cell cycle, was discovered through a unique screening system, in which effects of microbial metabolites on the cycle progression of the cultured mouse mammalian carcinoma cell were monitored by flow cytometry. The inhibitor was extracted from the fermentation broth of Streptomyces sp. ZF10 with ethyl acetate, and purified by silica gel column chromatography and HPLC.
( Suzuki Hirokazu ),( Shunji Takahashi ),( Hiroyuki Osada ),( Ken Ichi Yoshida ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.7
DNA methylation in Streptomyces griseus IFO 13350 was analyzed by high-performance liquid chromatographic analysis and bisulfite-based analysis to reveal two methylation sites, 5`-GC5mCGGC-3` and 5`-GAG5mCTC-3`. The methylation was reconstituted in Escherichia coli by simultaneous expression of S. griseus SGR4675 and S. achromogenes M.SacI. The E. coli cells produced plasmids that mimicked the methylation profile of S. griseus DNA, which was readily introduced into S. griseus. The results of this study raise the possibility of a promising approach to establish efficient transformation in several streptomycetes.
( Mariko Mochizuki ),( Yukari Osada ),( Nami Shimaoka ),( Mayumi Ito ),( Naomi Sannda ),( Junko Fujita ),( Hiroyuki Matumoto ),( Tadashi Matushita ) 대한임상병리사협회 2015 임상미생물검사학회 발표자료집 Vol.2015 No.-
Case The patient was a 45-year-old man. He was diagnosed as having malignant melanoma ten years ago, and received chemotherapy including alkylating agents. After nine years, he developed treatment-related myelodysplastic syndrome / myeloproliferative neoplasm (MDS / MPN), and he received hematopoietic stem cell transplantation. He had recurrence as MDS and re-transplanted. The blood culture after re-transplantation were repeatedly positive for Enterococci and yeast-like fungi of black-colored colony. Micafungin (MCFG) and Fluconazole were added to the treatment with liposomal-amphoterisin B. Hematopoetic stem cell transplantation was performed again due to bone marrow graft failure, however, his condition got worsened and died. Microbiological findings A yeast-like form with budding was found under microscopy, and black-colored colonies were grown on a CHROMagarTM Candida plate (Becton Dickinson). Species identification was not possible using VITEK and ApiC AUX (bioMerieux). Further culture revealed brown filamentous form on the melted chocolate-like colonies, which was strongly suggestive of dimorphic fungi. This fungus was identified as Exophiala dermatitidis by sequence analysis of ITS region of rRNA gene. Susceptibility test using the ASTY (KYOKUTO PHARMACEUTICAL INDUSTRIAL Co., Ltd) showed that, after 72hr growth, a high MIC values of > =16㎍/ml in MCFG and relatively good susceptibility to other antifungal drugs. Discussion Exophiala dermatitidis is a type of pathogenic dematiaceous fungi which was widely found in residential environment. It mainly causes infections of cutaneous and subcutaneous tissue in immunocompromised patients, but sometimes fatal infections such as bloodstream infection. In clinical settings, it is important to speculate this species from morphological features, which will lead to the early diagnosis and treatment especially in immunocompromised cases. Acknowledge Takashi Yaguchi, Aki Hirabayashi, Daizo Kato, Mitsutaka Iguchi, Satoshi Morioka, Tetsuya Yagi
Inhibition of protein tyrosine phosphatase 1B by lupeol and lupenone isolated from Sorbus commixta.
Na, Minkyun,Kim, Bo Yeon,Osada, Hiroyuki,Ahn, Jong Seog Taylor Francis 2009 Journal of enzyme inhibition and medicinal chemist Vol.24 No.4
<P>Protein tyrosine phosphatase 1B (PTP1B) appears to be an attractive target for the development of new drugs for type 2 diabetes and obesity. In our preliminary test, a MeOH extract of the stem barks of Sorbus commixta Hedl. (Rosaceae) showed strong PTP1B inhibitory activity. Bioassay-guided fractionation of the MeOH extract resulted in the isolation of two lupane-type triterpenes, lupenone (1) and lupeol (2). Compounds 1 and 2 inhibited PTP1B with IC(50) values of 13.7 +/- 2.1 and 5.6 +/- 0.9 microM, respectively. Kinetic studies revealed that both the compounds 1 and 2 are non-competitive inhibitors of PTP1B that decrease V(max) values with no effect on K(m) values.</P>
Hot springs and cool natural products
Kwon, Ho Jeong,Lee, Choong Hwan,Osada, Hiroyuki,Yoshida, Minoru,Imoto, Masaya Nature Publishing Group 2008 NATURE CHEMICAL BIOLOGY Vol.4 No.8
Natural products have played a unique role in providing new tools and insights in chemical biology. The tremendous value of natural products was highlighted by scientists from Korea and Japan at the 4<SUP>th</SUP> Korea-Japan Chemical Biology symposium.
Yukihiro Asami,김순옥,장준필,고성균,김보연,Hiroyuki Osada,장재혁,안종석 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.1
We previously identified a new heparinase inhibitor fungal metabolite, named CRM646-A, which showed inhibition of heparinase and telomerase activities in an in vitro enzyme assay and antimetastatic activity in a cell-based assay. In this study, we elucidated the mechanism by which CRM646-A rapidly induced nucleus condensation, plasma membrane disruption and morphological changes by increasing intracellular Ca2+ levels. Furthermore, PD98059, a mitogen-activated protein kinase (MEK) inhibitor, inhibited CRM646-A-induced nucleus condensation through ERK1/2 activation in rat 3Y1 fibroblast cells. We identified CRM646-A as a Ca2+ ionophore-like agent with a distinctly different chemical structure from that of previously reported Ca2+ ionophores. These results indicate that CRM646-A has the potential to be used as a new and effective antimetastatic drug.
The 7th Japan-Korea Chemical Biology Symposium: Chemical Biology of Natural Bioactive Molecules
Pandey, Ramesh Prasad,Kwon, Ho Jeong,Ahn, Jong Seog,Osada, Hiroyuki,Sohng, Jae Kyung American Chemical Society 2014 ACS CHEMICAL BIOLOGY Vol.9 No.5
<P>Natural bioactive molecules possess supreme chemical diversity and drug-like properties and are an important source for drug lead compounds. At the seventh Japan-Korea Chemical Biology Symposium at Jeju Island, Korea, chemical biologists from Korea and Japan highlighted the remarkable features of natural products and their significance.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/acbcct/2014/acbcct.2014.9.issue-5/cb5002558/production/images/medium/cb-2014-002558_0003.gif'></P>
RK-270D and E, Oxindole Derivatives from Streptomyces sp. with Anti-Angiogenic Activity
( Jun-pil Jang ),( Mina Jang ),( Toshihiko Nogawa ),( Shunji Takahashi ),( Hiroyuki Osada ),( Jong Seog Ahn ),( Sung-kyun Ko ),( Jae-hyuk Jang ) 한국미생물 · 생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.3
A chemical investigation of a culture extract from Streptomyces sp. RK85-270 led to the isolation and characterization of two new oxindoles, RK-270D (1) and E (2). The structures of 1 and 2 were determined by analyzing spectroscopic and spectrometric data from 1D and 2D NMR and High-resolution electrospray ionization mass spectrometry (HRESIMS) experiments. Compound 1 exhibited anti-angiogenic activities against human umbilical vein endothelial cells (HUVECs) without cytotoxicity. Results of Western blot analysis revealed that 1 inhibits VEGF-induced angiogenesis in the HUVECs via VEGFR2/ p38 MAPK-mediated pathway.