한국어 판 구조화 임상면담도구 개발 : 신뢰도 연구

한오수,안준호,송선희,조맹제,김장규,배재남,조성진,정범수,서동우,함봉진,이동우,박종익,홍진표 대한신경정신의학회 2000 신경정신의학 Vol.39 No.2

연구목적: 정신장애의 정확한 진단과 평가는 임상에서뿐만 아니라 신뢰성 있는 연구를 위해서도 매우 중요하다. Structured Clinical Interview for DSM-IV(SCID)는 임상전문가들이 사용하는 진단도구로서, 비교적 짧은 시간에 정확한 진단을 내릴 수 있다. 본 연구는 한국어판SCID를 개발하고 그 신뢰도를 평가하기 위해 수행되었다. 방법: 국문학자가 포함된 번역위원회를 통하여 연구용판 SCID를 번역한 후, 정신과 의사 2인과 임상심리학자 1인에게 한국어판SCID 실시방법을 교육시킨 뒤 한국어판SCID를 이용한 면담의 평가자간 신뢰도 (interrater reliability)를 검증하였다. 면담 대상은 1999년 2월에서 3월까지 2개 병원 정신과에 치료중인 환자 90명(남:41명, 여:29명)이었다. 결과: 현재 장애(current disorder) 평가에서 주요 우울장애, 기분부전장애, 정신분열병, 알코올 남용 및 의존, 기타 물질 남용 또는 의존, 여러 불안장애들 및 섭식장애 등과 같은 대부분 장애의 kappa값은 .70이상으로 매우 높았다. 그 이외의 양극성 장애, 망상장애, 광장공포증, 감별 불능 신체화 장애 및 건강염려증의 kappa값도 .69에서 .40사이로 수용할 수 있는 정도였다. 평생 장애(lifetime disorder)에서는 양극성 장애(k=.69)와 감별 불능 신체화장애(k=.59)를 제외한 다른 모든 장애의 kappa값이 .70이상이었다. K-SCID 면담시 Ⅰ축 질환에 대하여 흔하게 다중 진단이 내려졌으며, 평균 진단 수는 1.5∼1.7개로 나타났다. 결론: 한국어판 SCID는 신뢰도가 높은 진단도구로 생각되며, 향후 정신질환의 정확한 진단과 임상연구에 유용하게 사용될 수 있다. Objectives: Accurate diagnosis and assessment for psychiatric disorders is crucial for research, as well as for clinical practice. Structured Clinical Interview for DSM-Ⅳ(SCID-RV) is a less time-consumimg and more accurate structured diagnostic interview form. It can be used by clinical professions and is known for a reliable diagnostic tool. Present study was conducted to develop Korean version of SCID-RV and to test the inter-rater reliability. Methods: The authors have translated original SCID-RV into Korean, and revised in parallel with sociocultural background of Korea. Ninety patients from two psychiatric hospitals, both outpatient and inpatient, were interviewed and rated independently by three raters. Results: The kappa coefficients for most of illnesses, such as major depressive disorder, dysthymia, schizophrenia, alcohol abuse and dependency, anxiety disorder and eating disorder were excellent(>0.70) in the evaluation of current disorders. And the kappa coefficients for bipolar disorder, delusional disorder, agoraphobia, undifferentiated somatoform disorder, and hypochondriasis were acceptable(>0.40) in the evaluation of current disorders. In the evalua-tion of lifetime disorders, the concordant rates of all the diagnoses except bipolar disorder and undifferentiated somatoform disorder were excellent. Lack of hierarchy in DSM-Ⅳ allows for multiple Axis I diagnoses. Mean numbers of Axis I diagnoses per subject assigned by the three raters were 1.5-1.7. Conclusions: Our findings confirm that SCID-RV yields highly reliable diagnoses. SCID-RV is recommended for accurate diagnosis in clinical practice and research on psychiatric disorders.

효소면역측정법을 이용한 Fumonisin의 검출법 개발

손동화,한성민,임선희,이인원,조선희,강신영,이경애 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

Fumonisin에 대한 효소면역측정법(ELISA)을 개발하기 위하여 특이항체를 생산하고, 분석조건을 확립하여, 인위적으로 오염시킨 옥수수 시료의 분석을 행하였다. Fumonisin B_1 (FB_1)을 cholera toxin (CT)에 결합시킨 FB_1-CT conjugate를 면역원으로 하여 Freund's adjuvant와 함께, 또는 단독으로 두 군의 토끼에 면역하고 특이항체를 생산하였다. 항혈청을 ELISA로 분석한 결과 그 adjuvant와 함께 면역한 경우 항체역가(titer)가 높게 나타났다. 가장 높은 항체역가(1 : 16,000)를 나타낸 항혈청 및 그로부터 정제한 항체를 이용하여 간접법 및 직접법에 의한 경합적 ELISA(ciELISA 및 cdELISA)를 각각 확립하였다. 이 항체의 유사독소와의 교차반응을 ciELISA로 조사하였을 때, FB_3에 대한 교차반응율은 2%로 매우 낮았으나, FB_2에 대한 것은 179%로 다소 높았다. FB_1의 검출한계는 ciELISA에서 0.03ppb, cdELISA에서 0.3 ppb로 각각 나타났다. 실제 곡물시료의 분석에 ELISA 활용가능성을 조사하기 위하여 인위적으로 FB_1을 오염시킨 옥수수를 75% methanol로 추출하고 그 회수율을 측정한 결과, 분석이 불안정하여 회수율이 일정하게 나타나지 않았다. 그러나 방해물질의 제거를 위하여 시료추출액을 strong anion exchange(SAX) cartridge를 거쳐 세척한 다음 분석하였을 때, 3~10 ppm 범위의 FB_1 오염 옥수수에서 평균 34%(CV의 평균, 8.2%)의 안정된 회수율을 보였다. In order to develop enzyme-linked immunosorbent assay (ELISA) for fumonisins, production of specific antibodies, establishement of ELISA conditions, and quantitation of the toxin from spiked corns by ELISA were performed. Fumonisin B_1 (FB_1) conjugated to cholera toxin (CT) with or without Freund's adjuvant was subcutaneously injected into 2 groups of rabbits. When the titer of the antisera produced by each rabbit tested, higher titer was observed in case of the immunization with the adjuvant. By use of the antiserum showing the highest titer (1 : 16,000) and its purified antibodies, competitive indirect and direct ELISA's (ciELISA and cdELISA) were established, respectively. When the cross-reactivity of the antibody against fumonisin analogs was investigate by the ciELISA is was very low against B_3 (2%) but high against fumonisin B_2 (179%). The sensitivity of the ELISAs was also very high, because the detection limit for FB_1 was 0.03 ppb in ciELISA and 0.3 ppb in cdELISA. When the ELISA's were applied to the spiked corns after extraction with 75% methanol, the assay recovery of FB_1 was too unstable to assay. However, when cleanup by strong anion exchange (SAX) cartridge was introduced to remove interfering materials, the mean ELISA recovery of FB_1 from corns spiked to 3~10 ppm was found to be 34.0% and stable (mean of CV, 8.2%).

Identification of phytochrome-interacting protein candidates in Arabidopsis thaliana by co-immunoprecipitation coupled with MALDI-TOF MS

Phee, Bong-Kwan,Shin, Dong Ho,Cho, Jin-Hwan,Kim, Seong-Hee,Kim, Jeong-Il,Lee, Youn-Hyung,Jeon, Jong-Seong,Bhoo, Seong Hee,Hahn, Tae-Ryong WILEY-VCH 2006 Proteomics Vol. No.

<P>Phytochrome-interacting proteins have been extensively studied to elucidate light-signaling pathway in plants. However, most of these proteins have been identified by yeast two-hybrid screening using the C-terminal domain of phytochromes. We used co-immunoprecipitation followed by proteomic analysis in plant cell extracts in an attempt to screen for proteins interacting either directly or indirectly with native holophytochromes including the N-terminal domain as well as C-terminal domain. A total of 16 protein candidates were identified, and were selected from 2-DE experiments. Using MALDI-TOF MS analysis, 7 of these candidates were predicted to be putative phytochrome A-interacting proteins and the remaining ones to be phytochrome B-interacting proteins. Among these putative interacting proteins, protein phosphatase type 2C (PP2C) and a 66-kDa protein were strong candidates as novel phytochrome-interacting proteins, as knockout mutants for the genes encoding these two proteins had impaired light-signaling functions. A transgenic knockout Arabidopsis study showed that a 66-kDa protein candidate regulates hypocotyl elongation in a light-specific manner, and altered cotyledon development under white light during early developmental stages. The PP2C knockout plants also displayed light-specific changes in hypocotyl elongation. These results suggest that co-immunoprecipitation, followed by proteomic analysis, is a useful method for identifying novel interacting proteins and determining real protein-protein interactions in the cell.</P>

척추 골유합에 있어 황색 인대의 이중 역할: 골형성 유도 기능 및 체외 유전자 전달 매개체

문성환,김향,권언혜,김경희,윤홍기,김학선,한수봉,이환모 대한척추외과학회 2003 대한척추외과학회지 Vol.10 No.1

ADVANCED SFR DESIGN CONCEPTS AND R&D ACTIVITIES

Hahn, Do-Hee,Chang, Jin-Wook,Kim, Young-In,Kim, Yeong-Il,Lee, Chan-Bock,Kim, Seong-O,Lee, Jae-Han,Ha, Kwi-Seok,Kim, Byung-Ho,Lee, Yong-Bum Korean Nuclear Society 2009 Nuclear Engineering and Technology Vol.41 No.4

In order to meet the increasing demand for electricity, Korea has to rely on nuclear energy due to its poor natural resources. In order for nuclear energy to be expanded in its utilization, issues with uranium supply and waste management issues have to be addressed. Fast reactor system is one of the most promising options for electricity generation with its efficient utilization of uranium resources and reduction of radioactive waste, thus contributing to sustainable development. The Korea Atomic Energy Research Institute (KAERI) has been performing R&Ds on Sodium-cooled Fast Reactors (SFRs) under the national nuclear R&D program. Based on the experiences gained from the development of KALIMER conceptual designs of a pool-type U-TRU-10%Zr metal fuel loaded reactor, KAERI is currently developing Advanced SFR design concepts that can better meet the Generation IV technology goals. This also includes developing, Advanced SFR technologies necessary for its commercialization and basic key technologies, aiming at the conceptual design of an Advanced SFR by 2011. KAERI is making R&D efforts to develop advanced design concepts including a passive decay heat removal system and a supercritical $CO_2$ Brayton cycle energy conversion system, as well as developing design methodologies, computational tools, and sodium technology. The long-term Advanced SFR development plan will be carried out toward the construction of an Advanced SFR demonstration plant by 2028.

Enhancement of Isobutanol Production in Saccharomyces cerevisiae by Overexpressing the Basic Components of Iron-Sulfur Cluster Assembly

Seong-Hee PARK,Ji-Sook HAHN 한국생물공학회 2016 한국생물공학회 학술대회 Vol.2016 No.10

뇌졸중 후 편마비 환자의 삶의 질

편성범,김상한,한명수,권희규,이항재 大韓再活醫學會 1999 Annals of Rehabilitation Medicine Vol.23 No.2

Canine Distemper 백신의 비강내 접종에 의한 국소면역 유도

한웅,김성희,이두식,윤병수,임윤규 한국수의공중보건학회 2000 예방수의학회지 Vol.24 No.3

Management with a Combination of Prednisolone and Cyclosporine in a Dog with Granulomatous Meningoencephalitis

Seong Jun Kim,Young Sung Nam,Sa Hee Min,Ji Hwan Park,Tae Yeon Hahn,Tae Woo Kim,Soo Yeon Jeong,Hee Myung Park 대한수의학회 2012 대한수의학회 학술대회발표집 Vol.2012 No.-

CONCEPTUAL DESIGN OF THE SODIUM-COOLED FAST REACTOR KALIMER-600

Hahn, Do-Hee,Kim, Yeong-Il,Lee, Chan-Bock,Kim, Seong-O,Lee, Jae-Han,Lee, Yong-Bum,Kim, Byung-Ho,Jeong, Hae-Yong Korean Nuclear Society 2007 Nuclear Engineering and Technology Vol.39 No.3

The Korea Atomic Energy Research Institute has developed an advanced fast reactor concept, KALIMER-600, which satisfies the Generation IV reactor design goals of sustainability, economics, safety, and proliferation resistance. The concept enables an efficient utilization of uranium resources and a reduction of the radioactive waste. The core design has been developed with a strong emphasis on proliferation resistance by adopting a single enrichment fuel without blanket assemblies. In addition, a passive residual heat removal system, shortened intermediate heat-transport system piping and seismic isolation have been realized in the reactor system design as enhancements to its safety and economics. The inherent safety characteristics of the KALIMER-600 design have been confirmed by a safety analysis of its bounding events. Research on important thermal-hydraulic phenomena and sensing technologies were performed to support the design study. The integrity of the reactor head against creep fatigue was confirmed using a CFD method, and a model for density-wave instability in a helical-coiled steam generator was developed. Gas entrainment on an agitating pool surface was investigated and an experimental correlation on a critical entrainment condition was obtained. An experimental study on sodium-water reactions was also performed to validate the developed SELPSTA code, which predicts the data accurately. An acoustic leak detection method utilizing a neural network and signal processing units were developed and applied successfully for the detection of a signal up to a noise level of -20 dB. Waveguide sensor visualization technology is being developed to inspect the reactor internals and fuel subassemblies. These research and developmental efforts contribute significantly to enhance the safety, economics, and efficiency of the KALIMER-600 design concept.