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Photovoltaic Behavior of Dye-sensitized Long TiO<sub>2</sub> Nanotube Arrays
Kim, Sang-Mo,Kim, Hark-Jin,Kim, Yong-Joo,Lim, Goo-Il,Choi, Young-Sik,Lee, Wan-In Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.11
Long $TiO_2$ nanotube (NT) arrays, prepared by electrochemical anodization of Ti foils, have been utilized as dye-adsorbing electrodes in dye-sensitized solar cells (DSCs). By anodizing for 1-24 hr and subsequent annealing, highly crystallized and tightly-adhered NT arrays were tailored to 11-150 ${\mu}m$ lengths, ~90 nm innerpore diameter and ~30 nm wall thickness. I-V curves revealed that the photovoltaic conversion efficiency (${\eta}$) was proportional to the NT length up to 36 ${\mu}m$. Beyond this length, the ) was proportional to the NT length up to ${\eta}$ was still steadily increased, though at a much lower rate. For example, an ${\eta}$ of 5.05% at 36 ${\mu}m$ was increased to 6.18% at 150 ${\mu}m$. Transient photoelectron spectroscopic analyses indicated that NT array-based DSCs revealed considerably higher electron diffusion coefficient ($D_e$) and life time (${\tau}_e$) than those with $TiO_2$ nanoparticles (NP). Moreover, the electron diffusion lengths ($L_e$) of the photo-injected electrons were considerably larger than the corresponding NT lengths in all the cases, suggesting that electron transport in NT arrays is highly efficient, regardless of tube length.
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김인섭,Jung Eun Bae,Hark Mo Sung,Yong Kang,최용운 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.6
The purpose of this study was to evaluate the efficacy and mechanisms of the solvent/detergent (S/D) treatment, DEAE-toyopearl 650M anion-exchange column chromatography, heparin-sepharose 6FF affinity column chromatography, and Viresolve NFP filtration steps employed in the manufacture of high-purity antihemophilic factor IX (Green- Nine VF) from human plasma, with regard to removal and/or inactivation of blood-borne viruses. A variety of experimental model viruses for human pathogenic viruses, including human immunodeficiency virus (HIV), bovine herpes virus (BHV), bovine viral diarrhoea virus (BVDV), hepatitis A virus (HAV), murine encephalomyocarditis virus (EMCV), and porcine parvovirus (PPV), were all selected for this study. Samples from relevant stages of the production process were spiked with each virus and subjected to scale-down processes mimicking the manufacture of high-purity factor IX. Samples were collected at each step, immediately titrated using a 50% tissue culture infectious dose (TCID50), and virus reduction factors were evaluated. S/D treatment using the organic solvent, tri (n-butyl) phosphate (TNBP), and the detergent, Tween 80, was a robust and effective step in inactivation of enveloped viruses. Titers of HIV, BHV, and BVDV were reduced from the initial titer of 6.06, 7.72, and 6.92 log10 TCID50, respectively, reaching undetectable levels within 1 min of S/D treatment. DEAE-toyopearl 650M anion-exchange column chromatography was found to be a moderately effective step in the removal of HAV, EMCV, and PPV with log reduction factors of 1.12, 2.67, and 1.38, respectively. Heparin-sepharose 6FF affinity column chromatography was also moderately effective for partitioning BHV, BVDV, HAV, EMCV, and PPV with log reduction factors of 1.55, 1.35, 1.08, 1.19, and 1.61, respectively. The Viresolve NFP filtration step was a robust and effective step in removing all viruses tested, since HIV, BHV, BVDV, HAV, EMCV, and PPV were completely removed during the filtration step with log reduction factors of ≥ 5.51, ≥ 5.76, ≥ 5.18, ≥ 5.34, ≥ 6.13, and ≥ 4.28, respectively. Cumulative log reduction factors of HIV, BHV, BVDV, HAV, EMCV, and PPV were ≥ 10.52, ≥ 12.07, ≥ 10.49, ≥ 7.54, ≥ 9.99, and ≥ 7.24, respectively. These results indicate that the production process for GreenNine VF has a sufficient virus reduction capacity for achievement of a high margin of virus safety. The purpose of this study was to evaluate the efficacy and mechanisms of the solvent/detergent (S/D) treatment, DEAE-toyopearl 650M anion-exchange column chromatography, heparin-sepharose 6FF affinity column chromatography, and Viresolve NFP filtration steps employed in the manufacture of high-purity antihemophilic factor IX (Green- Nine VF) from human plasma, with regard to removal and/or inactivation of blood-borne viruses. A variety of experimental model viruses for human pathogenic viruses, including human immunodeficiency virus (HIV), bovine herpes virus (BHV), bovine viral diarrhoea virus (BVDV), hepatitis A virus (HAV), murine encephalomyocarditis virus (EMCV), and porcine parvovirus (PPV), were all selected for this study. Samples from relevant stages of the production process were spiked with each virus and subjected to scale-down processes mimicking the manufacture of high-purity factor IX. Samples were collected at each step, immediately titrated using a 50% tissue culture infectious dose (TCID50), and virus reduction factors were evaluated. S/D treatment using the organic solvent, tri (n-butyl) phosphate (TNBP), and the detergent, Tween 80, was a robust and effective step in inactivation of enveloped viruses. Titers of HIV, BHV, and BVDV were reduced from the initial titer of 6.06, 7.72, and 6.92 log10 TCID50, respectively, reaching undetectable levels within 1 min of S/D treatment. DEAE-toyopearl 650M anion-exchange column chromatography was found to be a moderately effective step in the removal of HAV, EMCV, and PPV with log reduction factors of 1.12, 2.67, and 1.38, respectively. Heparin-sepharose 6FF affinity column chromatography was also moderately effective for partitioning BHV, BVDV, HAV, EMCV, and PPV with log reduction factors of 1.55, 1.35, 1.08, 1.19, and 1.61, respectively. The Viresolve NFP filtration step was a robust and effective step in removing all viruses tested, since HIV, BHV, BVDV, HAV, EMCV, and PPV were completely removed during the filtration step with log reduction factors of ≥ 5.51, ≥ 5.76, ≥ 5.18, ≥ 5.34, ≥ 6.13, and ≥ 4.28, respectively. Cumulative log reduction factors of HIV, BHV, BVDV, HAV, EMCV, and PPV were ≥ 10.52, ≥ 12.07, ≥ 10.49, ≥ 7.54, ≥ 9.99, and ≥ 7.24, respectively. These results indicate that the production process for GreenNine VF has a sufficient virus reduction capacity for achievement of a high margin of virus safety.
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( Beom Su Kim ),( Hark Mo Sung ),( Hyung Keun You ),( Su Jin Kim ),( In Seop Kim ),( Jun Lee ) 한국조직공학·재생의학회 2011 조직공학과 재생의학 Vol.8 No.2
Mesenchymal stem cells (MSCs) and scaffolds may overcome the limitations of traditional bone grafts. We investigated growth and osteoblast differentiation of human alveolar bone marrow-derived MSCs (hABMMSCs) in fibrin gel (FG) 3-dimensional (3D) culture. We compared hABMMSCs with human iliac crestderived MSCs (hICMSCs) and alginate gel (AG) was compared with FG. Morphological, histological, cell proliferation and viability characteristics of 3D cultured hABMMSCs were defined and compared with hICMSCs and AG. Gel-embedded cells were cultured in medium containing osteogenic differentiation stimulant (OS) and differentiation was assessed by alkaline phosphate (ALP) activity and mRNA expression of osteoblast marker genes. Isolated hABMMSCs and hICMSCs exhibited fibroblast-like morphology and expressed CD44, CD73, CD, 90, CD105, and CD106, but not CD34. hABMMSC proliferation in 3D FG culture was about 3-fold greater than that in 3D AG culture. hABMMSCs and hICMSCs exhibited a rounded morphology in AG, and fibroblastic morphology in FG. OS treatment increased ALP activity in both hABMMSCs and hICMSCs cultured in FG but yielded no significant changes in AG. Activity reached a maximum on culture day 10, and decreased on day 15. Following OS treatment, mRNA expression of ALP and osteopontin (OPN) increased in hABMMSCs and hICMSCs in FG by 3-fold over that in AG. mRNA expression of collagen type Iα1 (ColIα1) and osterix (OSX) were also 2-fold and 6-fold greater in FG than in AG.
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Shin Jeong-Sup,Choi Yong-Woon,Sung Hark-Mo,Ryu Yeon-Woo,Kim In-Seop The Korean Society for Biotechnology and Bioengine 2006 Biotechnology and Bioprocess Engineering Vol.11 No.1
With particular regards to the hepatitis A virus (HAV), a terminal dry-heat treatment ($100^{\circ}C$ for 30 min) process, following lyophilization, was developed to improve the virus safety of a solvent/detergent-treated antihemophilic factor IX concentrate. The loss of factor IX activity during dry-heat treatment was of about 3%, as estimated by a clotting assay. No substantial changes were observed in the physical and biochemical characteristics of the dry-heat-treated factor IX compared with those of the factor IX before dry-heat treatment. The dry-heat-treated factor IX was stable for up to 24 months at $4^{\circ}C$, The dry-heat treatment after lyophilization was an effective process for inactivating viruses. The HAV and murine encephalomyocarditis virus (EMCV) were completely inactivated to below detectable levels within 10 min of the dry-heat treatment. Porcine parvovirus (PPV) and bovine herpes virus (BHV) were potentially sensitive to the treatment. The log reduction factors achieved during lyophilization and dry-heat treatment were ${\ge}5.60$ for HAV, ${\ge}6.08$ for EMCV, 2.64 for PPV, and 3.59 for BHV. These results indicate that dry-heat treatment improves the virus safety of factor IX concentrates, without destroying the activity. Moreover, the treatment represents an effective measure for the inactivation of non-lipid enveloped viruses, in particular HAV, which is resistant to solvent/detergent treatment.
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Kim, In-Seop,Park, Yong-Woon,Lee, Sung-Rae,Sung, Hark-Mo The Korean Society for Biotechnology and Bioengine 2004 Biotechnology and Bioprocess Engineering Vol.9 No.1
The purpose of the present study was to examine the efficacy and mechanism of fraction IV cold ethanol fractionation and pasteurization (60$^{\circ}C$ heat treatment for 10 h), involved in the manufacture of albumin from human plasma, in the removal and/or inactivation of the hepatitis A virus (HAV). Samples from the relevant stages of the production process were spiked with HAV and the amount of virus in each fraction then quantified using a 50% tissue culture infectious dose (TCID$\_$50/). HAV was effectively partitioned from albumin during the fraction IV cold ethanol fractionation with a log reduction factor of 3.43. Pasteurization was also found to be a robust and effective step in inactivating HAV, where the titers were reduced from an initial titer of 7.60 log TCID$\_$50/ to undetectable levels within 5 h of treatment. The log reduction factor achieved during pasteurization was $\geq$4.76. Therefore, the current results indicate that the production process for albumin has sufficient HAV reducing capacity to achieve a high margin of virus safety.
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김학상,김영모,박길흠,이광호,하영호,Kim, Hark-Sang,Kang, Young-Mo,Park, Kil-Houm,Lee, Kwang-Ho,Ha, Yeong-Ho 대한전자공학회 1988 전자공학회논문지 Vol. No.
영상에서 명암도의 변화가 큰 영역을 경계선이라 하며 이는 영상을 분류하고 해석하는 가장 기본적인 특징 중의 하나이다. 본 논문에서는 central moments에 의한 새로운 경계선 검출 방법을 제안하였다. 제안된 방법은 서로 다르게 정의된 확률변수 및 확률밀도함수와 moment의 차수에 따라 여러 가지 특성을 가진 경계선을 검출하였다. 또한 미분이 아닌 창내의 명암도의 평균과 각 화소와의 차를 적분한 연산자로서 기존의 연산자보다 잡음에 우수하며 가늘고 섬세한 경계선을 검출하였다. Edge is one of the primitive features of an image and is widely used in image classification and analysis. New edge extration methods using central moments are presented and show various characteristics according to the order of moment, definition of both random variables and probability density functions. The proposed methods use the integral of differences between local mean and pixels in the window whereas most of conventional edge operators use only differential concepts. This gives good noise immunity and extracts fine edges.
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김인섭,Yong Woon Choi,이성래,Hark Mo Sung 한국생물공학회 2004 Biotechnology and Bioprocess Engineering Vol.9 No.1
The purpose of the present study was to examine the efficacy and mechanism of fraction IV cold ethanol fractionation and pasteurization (60oC heat treatment for 10 h), involved in the manufacture of albumin from human plasma, in the removal and/or inactivation of the hepatitis A virus (HAV). Samples from the relevant stages of the production process were spiked with HAV and the amount of virus in each fraction then quantified using a 50% tissue culture infectious dose (TCID50). HAV was effectively partitioned from albumin during the fraction IV cold ethanol fractionation with a log reduction factor of 3.43. Pasteurization was also found to be a robust and effective step in inactivating HAV, where the titers were reduced from an initial titer of 7.60 log TCID50 to undetectable levels within 5 h of treatment. The log reduction factor achieved during pasteurization was 4.76. Therefore, the current results indicate that the production process for albumin has sufficient HAV reducing capacity to achieve a high margin of virus safety.
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