Sex Differences in Early and Late Recurrence after Liver Resection of Hepatocellular Carcinoma (A Multicenter Study from China)

( Jiong-jie Yu ),( Ju-dong Li ),( Xin-fei Xu ),( Zhen-li Li ),( Jun Han ),( Hao Xing ),( Han Wu ),( Jian-hong Zhong ),( Yi-sheng Huang ),( Ya- Hao Zhou ),( Ting-hao Chen ),( Hong Wang ),( Wei-min Gu ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: There is a striking sex difference in the incidence of hepatocellular carcinoma (HCC), with a strong predominance for males. However, the impact of sex on the incidence of recurrence after curative resection of HCC remains controversial. Herein, we assess sex differences in the risks of recurrence and mortality for patients undergone curative resection of HCC. Methods: Data from 1,435 HCC patients undergone curative resection (1,228 males and 207 females) between 2004 and 2014 at five institutions in China were retrospectively analyzed. Patients’ baseline characteristics, operative variables, and rates of early recurrence (≤ 2 years after resection), late recurrence (> 2 years) and cancer-specific mortality (CSM) were evaluated and compared. Multivariable competing-risks regression analyses were performed to identify predictors associated with CSM, early and late recurrence. Results: The early recurrence rates between males and females were similar (43.3% vs. 42.0%, P=0.728), but the late recurrence and CSM rates in males were higher when compared to females (17.2% vs. 11.2%, P=0.044; 42.8% vs. 34.3%, P=0.022). Multivariable competing-risks regression analyses revealed no sex difference in early recurrence; however, males had significantly higher late recurrence rate [hazard ratio (HR), 1.752; 95% CI, 1.145-2.682; P=0.010] and CSM rate (HR, 1.307; 95% CI, 1.015-1.683; P=0.038) than females. Conclusions: Males had significantly higher late recurrence and CSM rates after curative resection of HCC than females. This suggests postoperative surveillance for HCC recurrence be varied by sex, especially for patients without recurrence at 2 years after resection.

Networks of MicroRNAs and Genes in Retinoblastomas

Li, Jie,Xu, Zhi-Wen,Wang, Kun-Hao,Wang, Ning,Li, De-Qiang,Wang, Shang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Through years of effort, researchers have made notable progress in gene and microRNA fields about retinoblastoma morbidity. However, experimentally validated data for genes, microRNAs (miRNAs) and transcription factors (TFs) can only be found in a scattered form, which makes it difficult to conclude the relationship between genes and retinoblastoma systematically. In this study, we regarded genes, miRNAs and TFs as elements in the regulatory network and focused on the relationship between pairs of examples. In this way, we paid attention to all the elements macroscopically, instead of only researching one or several. To show regulatory relationships over genes, miRNAs and TFs clearly, we constructed 3 regulatory networks hierarchically, including a differentially expressed network, a related network and a global network, for analysis of similarities and comparison of differences. After construction of the three networks, important pathways were highlighted. We constructed an upstream and downstream element table of differentially expressed genes and miRNAs, in which we found self-adaption relations and circle-regulation. Our study systematically assessed factors in the pathogenesis of retinoblastoma and provided theoretical foundations for gene therapy researchers. In future studies, especial attention should be paid to the highlighted genes and miRNAs.

Effects of electromagnetic field and asymmetric Gaussian potential on low energy state energy of bound polaron in quantum well

Wang Ying-Hao,Chen Ying-Jie,Shao Feng-Lan 한국물리학회 2020 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.77 No.7

In this article, effects of electromagnetic field and asymmetric Gaussian potential (AGP) on the bound polaron's low energy state in quantum well are explored theoretically by the Lee-Low-Pines unitary transformation and Pekar type variational method. The variation of the ground state energy and the first excited state energy of the polaron with the Coulomb bound potential (CBP) strength at different electron-phonon coupling (EPC) constants, electric field (EF) strengths, heights and ranges of the AGP and magnetic field adjustment lengths are obtained. Our numerical results indicate that the polaron's low energy state depends on the EPC constant, the EF strength, the AGP's height and range and the CBP strength.

Development and characterization of a fully functional small anti-HER2 antibody

( Jie Gao ),( Bo Hua Le ),( Hui Mei Li ),( Xun Min Zhang ),( Da Peng Zhang ),( Lei Zhao ),( Chong Wang ),( Chen Fang ),( Wei Zhu Qian ),( Sheng Hou ),( Geng Kou ),( Hua Feng Wei ),( Shu Shi ),( Hao Wa 생화학분자생물학회 2009 BMB Reports Vol.42 No.10

Electrochemical Properties of Supercapacitors Using Boron Nitrogen Double-Doped Carbon Nanotubes as Conductive Additive

Hao Hu,Xiaogang Sun,Wei Chen,Jie Wang,Xu Li,Yapan Huang,Chengcheng Wei,Guodong Liang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.7

Carbon nanotubes (CNTs) were doped by ammonium borate as the sources of nitrogen and boron. Under the protection of Ar gas, boron-nitrogen doped CNTs were prepared through nitriding and boronization at high temperature. It is a conductive additive. Then, the obtained CNTs were mixed with activated carbon (AC), SP, sodium dodecyl sulfate (SDS), and cellulose fiber to prepare electrodes. With all the materials, a symmetric electric double-layer supercapacitor (EDLC) was assembled. Next, the materials and electrodes were also characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The factors, chemical connections, and specific surface area of the CNTs were analyzed by X-ray energy spectrum analysis (EDS), X-ray photoelectron spectroscopy (XPS), as well as a specific surface area and porosimetry analyzer (BET). In addition, the electrochemical performances of electric double-layer capacitors were tested with the help of cyclic voltammetry, constant-current charging and discharging, and so on. From the results, we can make a conclusion, that is, both B and N atoms were added into the CNTs and formed bonds successfully with carbon atoms mutually. Besides, the specific surface area is about 1.5 times than that of the CNT. When the charge/discharge current density reaches 50 mA/g, we can find that the mass specific capacitance of the capacitor can run up to 32.19 F/g. Also, we observe that the maximum power density is close to 220 W/kg (700 mA/g), and the energy density can arrive 9.31 Wh/kg (50 mA/g). Based on the impedance test, the electrodes are characterized with low impedance. After 2000 cycles, the boron-nitrogen doped double-layer capacitors maintain a capacitance retention ratio of above 95%. Its power density can still achieve 220 W/kg when the energy density keeps at 3.46 Wh/kg. In other words, the electrochemical performance functions of the electric double-layer capacitors are enhanced while the CNTs serve as the electrodes.

Channel Fading Effect Analysis on Diffusion Cooperation Strategies over Adaptive Networks

( Jie Yang ),( Ehsan Mostafapour ),( Amir Aminfar ),( Jie Wang ),( Hao Huang ),( Afsaneh Akhbari ),( C. Ghobadi ),( Guan Gui ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.1

In this paper, we investigate the performance of the diffusion adaptation strategies for parameter estimation in wireless adaptive networks, where the nodes exchange information over noisy and fading wireless channels. This paper shows the differences between the effect of Rayleigh and Rician fading over wireless adaptive networks and proves that the Rician fading is a more practical model in such kinds of networks. Simulation results imply that the effect of Rayleigh fading is more degrading for the estimation process than Rician fading. Also, the simulation results show the performance of adapt then combine (ATC) diffusion algorithm is better than the combine then adapt (CTA) algorithm by merely considering noise in wireless channels. While the performance of CTA prevails ATC over the wireless adaptive network in the presence of noise plus channel fading.

In Vitro and In Vivo Studies of Different Liposomes Containing Topotecan

Hao, Yan-Li,Deng, Ying-Jie,Chen, Yan,Wang, Xiu-Min,Zhong, Hai-Jun,Suo, Xu-Bin The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.5

Liposome as a carrier of topotecan (TPT), a promising anticancer drug, has been reported in attempt to improve the stability and antitumor activity of TPT. However, the biodistr ibution pattern of TPT liposome in vivo and PEG-modified liposome containing TPT have not been studied systemically. In this paper, the in vitro stability and in vivo biodistribution behavior of several liposomes containing TPT with different lipid compositions and PEG-modification were studied. Compared with the 'fluid' liposome (S-Lip) composed of soybean phosphatidylcholine (SPC), the 'solid' liposome (H-Lip) composed of hydrogenated soybean phosphatidylcholine HSPC decreased the leaking efficiency of TPT from liposome and enhanced the stability of liposome in fetal bovine serum (FBS) or human blood plasma (HBP). The results of biodistribution studies in S$_{180}$ tumor-bearing mice showed that liposomal encapsulation increased the concentrations of total TPT and the ratio of lactone form in plasma. Compared with free TPT, S-Lip and H-Lip resulted in 5- and 19- fold increase in the area under the curve (AUC$_{0\rightarrow\propto}$), respectively. PEG- modified H-Lip (H-PEG) showed 3.7-fold increase in AUC$_{0\rightarrow\propto}$ compared with H-Lip, but there was no significant increase in t$_{1/2}$ and AUC$_{0\rightarrow\propto}$ for PEG-modified S-Lip (S-PEG) compared with S-Lip. Moreover, the liposomal encapsulation changed the biodistribution behavior, and H-Lip and H-PEG dramatically increased the accumulation of TPT in tumor, and the relative tumor uptake ratios were 3.4 and 4.3 compared with free drug, respectively. There was also a marked increase in the distribution of TPT in lung when the drug was encapsulated into H-Lip and H-PEG. Moreover, H-PEG decreased the accumulation of TPT in bore marrow compared with unmodified H-Lip. All these results indicated that the membrane fluidity of liposome has an important effect on in vitro stability and in vivo biodistribution pattern of liposomes containing TPT, and PEG-modified 'solid' liposome may be an efficient carrier of TPT.

BF-30 effectively inhibits ciprofloxacin-resistant bacteria in vitro and in a rat model of vaginosis

Wang, Jing,Li, Bing,Li, Yang,Dou, Jie,Hao, Qingru,Tian, Yuwei,Wang, Hui,Zhou, Changlin 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.7

Bacterial infections are becoming increasingly difficult to treat due to the increasing number of multidrug-resistant strains. Cathelicidin-BF (BF-30) is a cathelicidin-like antimicrobial peptide and exhibits broad antimicrobial activity against bacteria. In the present study, the antibacterial activity of BF-30 against ciprofloxacin-resistant Escherichia coli and Staphylococcus aureus was examined, and the protective effects of this peptide against these bacteria in rats with bacterial vaginosis were identified for the first time. The data showed that BF-30 had effective antimicrobial activities against ciprofloxacin-resistant E. coli and S. aureus. The minimal inhibitory concentrations for both bacterial strains were $16{\mu}g/ml$, and the minimal bactericidal concentrations were 64 and $128{\mu}g/ml$, respectively. A time course experiment showed that the CFU counts rapidly decreased after BF-30 treatment, and the bacteria were nearly eliminated within 4 h. BF-30 could reduce the fold change (CFU/ml) in local colonization by drug-resistant E. coli and S. aureus to 0.01 at a dose of 0.8 mg/kg/day in the rats' vaginal secretions. In addition, BF-30 induced membrane permeabilization and bound to the genomic DNA, interrupting protein synthesis. Taken together, our data demonstrate that BF-30 has potential therapeutic value for the prevention and treatment of bacterial vaginosis.

Biochemical Characterization of a Psychrophilic Phytase from an Artificially Cultivable Morel Morchella importuna

( Hao Tan ),( Jie Tang ),( Xiaolin Li ),( Tianhai Liu ),( Renyun Miao ),( Zhongqian Huang ),( Yong Wang ),( Bingcheng Gan ),( Weihong Peng ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.12

Psychrophilic phytases suitable for aquaculture are rare. In this study, a phytase of the histidine acid phosphatase (HAP) family was identified in Morchella importuna, a psychrophilic mushroom. The phytase showed 38% identity with Aspergillus niger PhyB, which was the closest hit. The M. importuna phytase was overexpressed in Pichia pastoris, purified, and characterized. The phytase had an optimum temperature at 25°C, which is the lowest among all the known phytases to our best knowledge. The optimum pH (6.5) is higher than most of the known HAP phytases, which is fit for the weak acidic condition in fish gut. At the optimum pH and temperature, MiPhyA showed the maximum activity level (2,384.6 ± 90.4 μmol·min^-1·mg^-1, suggesting that the enzyme possesses a higher activity level over many known phytases at low temperatures. The phytate-degrading efficacy was tested on three common feed materials (soybean meal/rapeseed meal/corn meal) and was compared with the well-known phytases of Escherichia coli and A. niger. When using the same amount of activity units, MiPhyA could yield at least 3× more inorganic phosphate than the two reference phytases. When using the same weight of protein, MiPhyA could yield at least 5× more inorganic phosphate than the other two. Since it could degrade phytate in feed materials efficiently under low temperature and weak acidic conditions, which are common for aquacultural application, MiPhyA might be a promising candidate as a feed additive enzyme.

Induced expression of three heat shock proteins mediated by thermal stress in Heortia vitessoides (Lepidoptera: Crambidae)

Jie Cheng,Chun-Yan WANG,Zi-Hao LYU,Tong Lin 한국곤충학회 2018 Entomological Research Vol.48 No.5

To gain an insight into the function of heat shock proteins (HSPs) in insects during thermal stress, three HSP cDNAs were identified in the transcriptome of adult Heortia vitessoides, one of the most destructive defoliating pests in Aquilaria sinensis (Loureiro) Sprenger forests. The open reading frames of HvHsp60, HvHsp70, and HvHsp90 were 1,719, 2,070, and 2,151 bp in length, respectively, and encoded proteins with molecular weights of 61.05, 75.02, and 82.23 kDa, respectively. Sequence analysis revealed that all three HSPs were highly conserved in structure. Regarding the stage‐specific expression profiles, HvHsp60, HvHsp70, and HvHsp90 mRNAs were detected in all developmental stages. Regarding the tissue‐specific expression profiles, the expression levels of the three HSP genes were different in various larval and adult tissues. Moreover, the expression patterns of heat‐stressed larvae, pupae, and adults indicated that HvHsp60, HvHsp70, and HvHsp90 were heat‐inducible. In particular, HvHsp60 transcripts increased dramatically in larvae and pupae that were heat‐stressed at 40°C and were upregulated in adults that were heat‐stressed at 35°C and 40°C. The expression of HvHsp70 significantly increased in all of the three different developmental stages at 35°C, 40°C, and 45°C. The expression of HvHsp90 obviously increased at 30°C, 35°C, and 40°C in larvae and could be induced at 35°C in pupae and adults. The results suggest that HSP60, HSP70, and HSP90 play a major role in protecting H. vitessoides against high‐temperature stress.