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Research on Damage Identification of Buried Pipeline Based on Fiber Optic Vibration Signal
Weihong Lin,Wei Peng,Yong Kong,Zimin Shen,Yuzhou Du,Leihong Zhang,Dawei Zhang 한국광학회 2023 Current Optics and Photonics Vol.7 No.5
Pipelines play an important role in urban water supply and drainage, oil and gas transmission, etc. This paper presents a technique for pattern recognition of fiber optic vibration signals collected by a distributed vibration sensing (DVS) system using a deep learning residual network (ResNet). The optical fiber is laid on the pipeline, and the signal is collected by the DVS system and converted into a 64 × 64 single-channel grayscale image. The grayscale image is input into the ResNet to extract features, and finally the K-nearest-neighbors (KNN) algorithm is used to achieve the classification and recognition of pipeline damage.
Weihong Qin,Wang Song,Peng Feng,Zhuo Xi,Tongqing Zhang 국제구조공학회 2023 Structural Engineering and Mechanics, An Int'l Jou Vol.85 No.1
To explore the effect of Engineered Cementitious Composite (ECC) on improving the progressive collapse resistance of reinforced concrete frames under a middle column removal scenario, six beam-column substructures were tested by quasistatic vertical loading. Among the six specimens, four were ECC-concrete composite specimens consisting of different depth of ECC at the bottom or top of the beam and concrete in the rest of the beam, while the other two are ordinary reinforced concrete specimens with different concrete strength grades for comparison. The experimental results demonstrated that ECC-concrete composite specimens can improve the bearing capacity of a beam-column substructure at the stages of compressive arch action (CAA) and catenary action in comparison with ordinary concrete specimen. Under the same depth of ECC, the progressive collapse resistance of a specimen with ECC at the beam bottom was superior to that at the beam top. With the increase of the proportion of ECC arranged at the beam bottom, the bearing capacity of a composite substructure was increased, but the increase rate slows down with the proportion. Meanwhile, the nonlinear numerical analysis software MSC Marc was used to simulate the whole loading process of the six specimens. Theoretical formulas to calculate the capacities of ECC-concrete composite specimens at the stages of flexural action, CAA and catenary action are proposed. Based on the research results, this study suggests that ECC should be laid out at the beam bottom and the layout depth should be within 25% of the total beam depth.
Tan, Hao,Wu, Xiang,Xie, Liyuan,Huang, Zhongqian,Gan, Bingcheng,Peng, Weihong The Korean Society for Microbiology and Biotechnol 2015 Journal of microbiology and biotechnology Vol.25 No.6
A phytase gene was identified in a publicly available metagenome derived from subsurface groundwater, which was deduced to encode for a protein of the histidine acid phosphatase (HAP) family. The nucleotide sequence of the phytase gene was chemically synthesized and cloned, in order to further overexpress the phytase in Escherichia coli. Purified protein of the recombinant phytase demonstrated an activity for phytic acid of 298 ± 17 µmol P/min/mg, at the pH optimum of 2.0 with the temperature of 37℃. Interestingly, the pH optimum of this phytase is much lower in comparison with most HAP phytases known to date. It suggests that the phytase could possess improved adaptability to the low pH condition caused by the gastric acid in livestock and poultry stomachs.
( Xiaoping Zhang ),( Bo Zhang ),( Renyun Miao ),( Jie Zhou ),( Lei Ye ),( Dinghong Jia ),( Weihong Peng ),( Lijuan Yan ),( Xiaoping Zhang ),( Wei Tan ),( Xiaolin Li ) 한국균학회 2018 Mycobiology Vol.46 No.3
Temperature is an important environmental factor that can greatly influence the cultivation of Auricularia cornea. In this study, lignin peroxidase, laccase, manganese peroxidase, and cellulose in A. cornea fruiting bodies were tested under five different temperatures (20℃, 25℃, 30℃, 35℃, and 40℃) in three different culture periods (10 days, 20 days and 30 days). In addition, the V4 region of bacterial 16S rRNA genes in the substrate of A. cornea cultivated for 30 days at different temperatures were sequenced using next-generation sequencing technology to explore the structure and diversity of bacterial communities in the substrate. Temperature and culture days had a significant effect on the activities of the four enzymes, and changes in activity were not synchronized with changes in temperature and culture days. Overall, we obtained 487,694 sequences from 15 samples and assigned them to 16 bacterial phyla. Bacterial community composition and structure in the substrate changed when the temperature was above 35℃. The relative abundances of some bacteria were significantly affected by temperature. A total of 35 genera at five temperatures in the substrate were correlated, and 41 functional pathways were predicted in the study. Bacterial genes associated with the membrane transport pathway had the highest average abundance (16.16%), and this increased at 35℃ and 40℃. Generally, different temperatures had impacts on the physiological activity of A. cornea and the bacterial community in the substrate; therefore, the data presented herein should facilitate cultivation of A. cornea.
( Hao Tan ),( Jie Tang ),( Xiaolin Li ),( Tianhai Liu ),( Renyun Miao ),( Zhongqian Huang ),( Yong Wang ),( Bingcheng Gan ),( Weihong Peng ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.12
Psychrophilic phytases suitable for aquaculture are rare. In this study, a phytase of the histidine acid phosphatase (HAP) family was identified in Morchella importuna, a psychrophilic mushroom. The phytase showed 38% identity with Aspergillus niger PhyB, which was the closest hit. The M. importuna phytase was overexpressed in Pichia pastoris, purified, and characterized. The phytase had an optimum temperature at 25°C, which is the lowest among all the known phytases to our best knowledge. The optimum pH (6.5) is higher than most of the known HAP phytases, which is fit for the weak acidic condition in fish gut. At the optimum pH and temperature, MiPhyA showed the maximum activity level (2,384.6 ± 90.4 μmol·min<sup>-1</sup>·mg<sup>-1</sup>, suggesting that the enzyme possesses a higher activity level over many known phytases at low temperatures. The phytate-degrading efficacy was tested on three common feed materials (soybean meal/rapeseed meal/corn meal) and was compared with the well-known phytases of Escherichia coli and A. niger. When using the same amount of activity units, MiPhyA could yield at least 3× more inorganic phosphate than the two reference phytases. When using the same weight of protein, MiPhyA could yield at least 5× more inorganic phosphate than the other two. Since it could degrade phytate in feed materials efficiently under low temperature and weak acidic conditions, which are common for aquacultural application, MiPhyA might be a promising candidate as a feed additive enzyme.
( Hao Tan ),( Renyun Miao ),( Tianhai Liu ),( Xuelian Cao ),( Xiang Wu ),( Liyuan Xie ),( Zhongqian Huang ),( Weihong Peng ),( Bingcheng Gan ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.10
A novel phytase of Acidobacteria was identified from a soil metagenome, cloned, overexpressed, and purified. It has low sequence similarity (<44%) to all the known phytases. At the optimum pH (2.5), the phytase shows an activity level of 1,792 μmol/min/mg at physiological temperature (37°C) and could retain 92% residual activity after 30 min, indicating the phytase is acidophilic and acidostable. However the phytase shows poor stability at high temperatures. To improve its thermal resistance, the enzyme was redesigned using Disulfide by Design 2.0, introducing four additional disulfide bridges. The half-life time of the engineered phytase at 60°C and 80°C, respectively, is 3.0× and 2.8× longer than the wild-type, and its activity and acidostability are not significantly affected.