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Jie Cheng,Jingxiang Chen,Tong Lin 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4
Heortia vitessoides Moore, a defoliating pest in Aquilaria sinensis (Loureiro) Sprenger forests, is responsible for significant damage to the trees. There is a lack of nucleotide and protein sequence information for H. vitessoides. Hence, in this study, the transcriptome of H. vitessoides was sequenced using the Illumina Hiseq 2000 platform to evaluate the expression profiles and predict the functional genes. A total of 60,587,900 clean reads were produced and de novo assembled into 66,673 unigenes of average length 957 bp; 28,202 and 27,339 identified unigenes were annotated using the National Center for Biotechnology Information (NCBI) non-redundant (NR) database and Swiss-Prot database, respectively. All assembled unigenes were categorized into 64 biological processes, cellular components, or molecular functions using Gene Ontology (GO). In total, 24,278 unigenes were annotated using a database of eukaryotic orthologous groups (KOG) and assigned to 25 functional categories. Of these, 9172 unigenes had matches in the Kyoto Encyclopedia of Genes and Genomes (KEGG) and were assigned to 344 KEGG pathways. In addition, there were 11,670 simple sequence repeats (SSRs) in 66,673 unigenes and 64 repeated motif types, of which A/T was the most frequent. We also found that 1000 unigenes were related to insecticide resistance. To the best of our knowledge, this is the first comprehensive transcriptome analysis for H. vitessoides, providing valuable genome data sources for the study of the molecular biology of this pest
Cheng, Jie,Park, Minsung,Hyun, Jinho The Royal Society of Chemistry 2014 Chemical communications Vol.50 No.22
<P>Thermoresponsive biopolymeric microstructures are prepared by exploiting the thermal phase transitions of emulsified elastin-like polypeptides (ELPs). ELP microbeads prepared below the transition temperature (<I>T</I><SUB>t</SUB>) have positively thermoresponsive pores. ELP microcapsules prepared above <I>T</I><SUB>t</SUB> have negatively thermoresponsive pores. Interestingly, the ELP microcapsules prepared at the temperature of the transition state displayed bidirectional thermoresponsive behavior.</P> <P>Graphic Abstract</P><P>The responsiveness and morphology of ELP microstructures were controlled through the temperature used during the crosslinking process in an emulsion. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3cc49044k'> </P>
Electrospinning versus microfluidic spinning of functional fibers for biomedical applications
Cheng, Jie,Jun, Yesl,Qin, Jianhua,Lee, Sang-Hoon Elsevier 2017 Biomaterials Vol.114 No.-
<P><B>Abstract</B></P> <P>Micro- or nanofiber-based materials have extensive applications in biomedical fields due to their capability to mimic many aspects of physiological microenvironment <I>in vivo</I>. Fabricating micro- or nanofibers using biocompatible and biodegradable materials is becoming of great interest in the area of biomaterials and tissue engineering. Among the various technologies, electrospinning and microfluidic spinning are the two promising approaches to produce fibers at micro- and nano-scale. Choosing an appropriate spinning method is critical important for a specific application. Although some review papers on each spinning method have been published, a review comparing these two methods has not been reported yet. In this review, we present an overview of the two spinning methods including the spinning principle, their unique features and materials selections. Several applications of fibers spun by both methods, especially in tissue engineering, organ function regeneration and drug delivery are introduced. The current challenges, future directions and potential applications of these approaches are discussed as well.</P>
Cheng Jie,Wei Fan,Zhang Mingfei,Li Nan,Song Tianqi,Wang Yong,Chen Dongsheng,Xiang Jishan,Zhang Xiaoke 한국유전학회 2021 Genes & Genomics Vol.43 No.9
Background Cloning and characterizing the drought-inducible promoters is essential for their use in crop resistance’s genetic improvement. Previous studies have shown that the TaNRX1-D gene participates in regulating the response of wheat to drought stress. However, its promoter has not yet been identifed. Objective In this study, we aimed to characterize the promoter of the TaNRX1-D gene. Methods The promoter of TaNRX1-D (named P0, 2081 bp) was isolated from common wheat with several cis-acting elements that regulate in response to abiotic stresses and some core cis-acting elements. Functional verifcation of the promoter, eight 5′-deletion fragments of TaNRX1-D promoter, was fused to the β-glucuronidase (GUS) gene P0::GUS~P7::GUS and transformed into Arabidopsis, respectively. Agrobacterium-mediated GUS transient assay the P6a and P6b promoter regions in tobacco leaves under normal, osmotic or ABA stress. Results Activity analysis of the full-length promoter (P0) showed that the intensity of stronger β-glucuronidase (GUS) staining in the roots and leaves was obtained during the growth of transgenic Arabidopsis. P0::GUS displayed the GUS activity was much higher in the roots and leaves than in other parts of the transgenic plant under normal conditions, which was similarly within wheat. Analysis of the 5′-deletion fragments revealed that P0::GUS~P6::GUS responded well upon exposure to osmotic (polyethylene glycol-6000, PEG6000) and abscisic acid (ABA) stress treatments and expressed signifcantly higher GUS activity than the CaMV35S promoter (35S::GUS), while P7::GUS did not. GUS transient assay in tobacco leaves showed that the GUS activities of P6a and P6b were lower than P6 in the PEG6000 and ABA stresses. Conclusion The 193 bp (P6) segment was considered the core region of TaNRX1-D responding to PEG6000 or ABA treatment. GUS activity assay in transgenic Arabidopsis showed that this segment was sufcient for the PEG6000 or ABA stress response. The identifed 193 bp promoter of TaNRX1-D in this study will help breed osmotic or ABA tolerant crops. The 36 bp segment between P6 and P6b (−193 to −157 bp) was considered the critical sequence for the TaNRX1-D gene responding to PEG6000 or ABA treatment.
Bicluster and Pathway Enrichment Analysis of HCV-induced Cirrhosis and Hepatocellular Carcinoma
Cheng, Peng,Cheng, You,Su, Mei X.,Li, Dong,Zhao, Guo Z.,Gao, Hui,Li, Yan,Zhu, Jie Y.,Li, Hua,Zhang, Tao Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8
Background: Hepatocellular carcinoma (HCC) is the sixth most common cancer worldwide and the most common form of liver cancer. However, while it is associated frequently with hepatitis C virus (HCV) there is only an elementary understanding of its molecular pathogenesis. Methods: To gain insight into the molecular mechanisms of HCV-induced hepatocarcinogenesis, we performed microarray analysis on 75 surgical liver samples from 48 HCV-infected patients. Results: There were 395 differentially expressed geness between cirrhotic samples and HCC samples. Of these, 125 genes were up-regulated and 270 genes were down-regulated. We performed pathway enrichment analysis and screened as described previously. Conclusions: The differentially expressed genes might be involved in hepatocarcinogenesis through upregulating the pathways of ECM-receptor interaction, focal adhesion, cell adhesion molecules and other cancer-related pathways, and downregulating the pathways of "complement and coagulation cascades". We hope our results could aid in seeking of therapeutic targets for HCV-induced hepatocellular carcinoma.