Kidney Toxicity Induced by 13 Weeks Exposure to the Fruiting Body of Paecilomyces sinclairii in Rats

Jeong, Mi-Hye,Kim, Young-Won,Min, Jeong-Ran,Kwon, Min,Han, Beom-Suk,Kim, Jeong-Gyu,Jeong, Sang-Hee Korean Society of ToxicologyKorea Environmental Mu 2012 Toxicological Research Vol.28 No.3

Paecilomyces sinclairiis (PS) is known as a functional food or human health supplement. However concerns have been raised about its kidney toxicity. This study was performed to investigate the kidney toxicity of PS by 13 week-oral administration to rats. Blood urea nitrogen (BUN), serum creatinine, and kidney damage biomarkers including beta-2-microglobulin (${\beta}2m$), glutathione S-transferase alpha (GST-${\alpha}$), kidney injury molecule 1 (KIM-1), tissue inhibitor of matrix metalloproteinase 1 (TIMP-1), vascular endothelial growth factor (VEGF), calbindin, clusterin, cystatin C, neutrophil gelatinase-associated lipocalin (NGAL) and osteopontin were measured during or after the treatment of PS. BUN, creatinine and kidney damage biomarkers in serum were not changed by PS. However, kidney cell karyomegaly and tubular hypertrophy were observed dose-dependently with higher severity in males. KIM-1, TIMP-1 and osteopontin in kidney and urine were increased dose dependently in male or at the highest dose in female rats. Increased urinary osteopontin by PS was not recovered at 2 weeks of post-exposure in both genders. Cystatin C in kidney was decreased at all treatment groups but inversely increased in urine. The changes in kidney damage biomarkers were more remarkable in male than female rats. These data indicate that the PS may provoke renal cell damage and glomerular filtration dysfunction in rats with histopathological lesions and change of kidney damage biomarkers in kidney or urine. Kidney and urinary KIM-1 and cystatin C were the most marked indicators, while kidney weight, BUN and creatinine and kidney damage biomarkers in serum were not influenced.

Kidney Toxicity Induced by 13 Weeks Exposure to the Fruiting Body of Paecilomyces sinclairii in Rats

Mihye Jeong,Young-Won Kim,Jeong-Ran Min,Min Kwon,Beom-Suk Han,Jeong-Gyu Kim,Sang-Hee Jeong 한국독성학회 2012 Toxicological Research Vol.28 No.3

Paecilomyces sinclairiis (PS) is known as a functional food or human health supplement. However concerns have been raised about its kidney toxicity. This study was performed to investigate the kidney toxicity of PS by 13 week-oral administration to rats. Blood urea nitrogen (BUN), serum creatinine, and kidney damage biomarkers including beta-2-microglobulin (β2m), glutathione S-transferase alpha (GST-α), kidney injury molecule 1 (KIM-1), tissue inhibitor of matrix metalloproteinase 1 (TIMP-1), vascular endothelial growth factor (VEGF), calbindin, clusterin, cystatin C, neutrophil gelatinase-associated lipocalin (NGAL) and osteopontin were measured during or after the treatment of PS. BUN, creatinine and kidney damage biomarkers in serum were not changed by PS. However, kidney cell karyomegaly and tubular hypertrophy were observed dose-dependently with higher severity in males. KIM-1, TIMP-1 and osteopontin in kidney and urine were increased dose dependently in male or at the highest dose in female rats. Increased urinary osteopontin by PS was not recovered at 2 weeks of post-exposure in both genders. Cystatin C in kidney was decreased at all treatment groups but inversely increased in urine. The changes in kidney damage biomarkers were more remarkable in male than female rats. These data indicate that the PS may provoke renal cell damage and glomerular filtration dysfunction in rats with histopathological lesions and change of kidney damage biomarkers in kidney or urine. Kidney and urinary KIM-1 and cystatin C were the most marked indicators, while kidney weight, BUN and creatinine and kidney damage biomarkers in serum were not influenced.

열렌즈 효과가 Z-자형 Ti:Sapphire 레이저의 안정성과 비점수차 보정에 미치는 영향

김동익,김규욱,추한태,전윤환 金烏工科大學校 1999 論文集 Vol.20 No.-

We have used ABCD transfer matrix to investigate stability of Z-fold Ti:sapphire laser cavity. Especially, we have examined the effects of radius of concave mirrors and thermal lensing due to pumping laser power on the stability of laser cavity, beam size, astigmatic compensation, etc.

생화학적 표식자를 이용한 골다공증의 평가

이규한,박영재,김미정,이상건,이강목 大韓再活醫學會 1998 Annals of Rehabilitation Medicine Vol.22 No.3

Electrophoresis에 의한 무 發芽時 子葉內의 蛋白質 및 Peroxidase의 變化에 關한 硏究

韓圭相,金載英 建國大學校 附設 自然科學硏究所 1993 建國自然科學硏究誌 Vol.4 No.-

무 발아시의 종자 저장단백질과 isoperoxides의 변화를 전기영동을 이용하여 조사하였다. 단백질은 14% SDS-Polyarylamide를 사용하여 파종 후 1일부터 8일까지의 변화를 조사하였고, isoperoxides는 10% Polyacrylmide를 사용하여 종자와 파종 후 2,5,9,11 및 13일경의 상태를 조사하였던 바 무의 저장단백질은 분자량이 32kDa와 30.5kDa, 27.5kDa의 3가지 polypeptides로 추정되며, 저장단백질은 파종 후 3일경에는 소화작용이 끝나 대부분이 저 분자량의 polypeptides로 변하는데, 32kDa의 polypeptides가 먼저 분해되었다. 파종 후 5일경에는 子葉이 완전히 전개된 상태로 대부분의 저장단백질이 소모되는 것으로 추정된다. Peroxidase는 파종 후 5일경과, 파종 후 9일경에 동위효소의 수가 변하는데 이는 자엽의 동화작용이 시작하는 시기와 자엽이 노화하는 것과 연관이 있는 것 같다. 파종후 7일 된 무 苗에 4℃에서 5일간 저온 처리하여 이들의 단백질의 변화와 peroxidase의 활성을 조사한 결과 Peroxidase는 저온처리로 동위효소의 수가 증가하였다. 단백질은 저온처리 한 개체와 저온처리하지 않은 개체간에 매우 적은 변화만 보였다. The changes of storage protein and isoperoxidase of radish cotyledons during germination was studied. Storage protein was separated with 14% SDS-polyacry1amide. Isoperoxidase was separated with 10% polyacrylamide. The radish seedling's storage protein was extracted every day from 1 to 8 days after sowing. Isoperoxidse of seeds and seedlings of 2,5,9,11 and 13 days after sowing was extracted. And 7-day old seedlings of radish were transferred to 4℃ for 5 days, and the changes of isoperoxidase and protein banding pattern were also studied. The molecular weight of storage protein of radish seeds were approximately 32kDa, 30.5kDa, and 27.5kDa respectively. The invertion of storage protein of radish seeds to low molecular weight polypeptides finished about 3 days after sowing when the root hair begin to emerge. 32kDa polypeptides was inverted to lower molecular weight polypeptides more faster than 27.5kDa and 30.5kDa polypeptides. It seems that storage protein of radish seeds almost consumed about 5 days after sowing when the cotyledons of radish has expended fully. The numbers of isoperoxidase changed 5 days and 9 days after sowing, respectively. The cotyledons of radish begins to greenish at 5 days after sowing and becomes yellowish 9 days after sowing. Isoperoxidases were increased by low temperature treatment. But the changes of protein banding bettern between the seedlings treated with low temperature and the seedlings nontreated with low temperature was not clear.

역설계 시스템에 의한 자동차용 프레스 제품의 검사

김수용,한민식,김태호,조규재,전언찬 朝鮮大學校 機械技術硏究所 2005 機械技術硏究 Vol.8 No.2

This study is an inspection of press forming product and mould using reverse engineering system. The inspection process on production field involves a lot of errors because of the hand-work so we focussed on improving the measured precision through performing the effective inspection using 3D non-contact scanner. By so doing that. we improved the precision of press forming product by analyzing the cause following the inspection result. Through the inspection, we applied it to the reverse engineering and we could improve the inspection process.

반도체 레이저로 펌핑하는 Nd:YVO₄레이저와 Cr:YAG 결정을 이용한 수동형 Q-Switching 특성

김규욱,추한태 금오공과대학교 산업기술개발연구원 2001 産業技術開發硏究 Vol.17 No.-

We report the characteristics of laser diode pumped Nd:YVO₄laser and its passive Q-Switching by using a Cr:YAG crystal. We have investigated the effects of cavity length of the Nd:YVO₄laser, pumping power of the laser diode, and transmission of the Cr:YAG crystal on the repetition rate and the width of Q-Switchied pulses. The periodic doubling phenomena were observed not only in the Q-Switchied pulses but also in the intracavity Q-Switching second harmonics which were generated by using a KTP nonlinear crystal.

델타변조방식을 이용한 전류형 능동필터의 PWM 신호 발생

김한성,김홍성,최규하,신우석 建國大學校 附設 産業技術硏究所 1993 논문집 Vol.18 No.-

The Delta-Modulation(DM) techniques have been used up to now for both UPS and AC motor drives, which have the advantages of simple circuit, fast response, and insensitivity to load variation and other disturbances. In this paper, the DM techniques are adopted to obtain PWM control signals for active power filters. In the design of delta modluator, the decision of break frequency is the most important, particularly for active power filter application. Both digital simulation and simple experiment are performed to investigate the characteristics of the delta modulator proposed.

Thyristor를 利用한 交流電流 制御方式에 關한 解析

金漢星,崔圭夏 건국대학교 부설 산업기술연구소 1982 논문집 Vol.7 No.-

To improve the faults of phase control, this paper suggests a new approach to the power control of R-L load with AC supply. AC current control can make the load current continuous during each operation cycle by restricting the magnitude of load current within the given upper and lower reference currents. Therefore the harmonic contents of load current can be greatly reduced and the distortion factor of the output waveform can be better than that of phase control. Digital computer simulation reveals that the AC current control scheme is superior to the phase control scheme in controlling the power flow into the load R-L and experimental results demonstrate the feasibility and verify the operation of the AC current control scheme.

천연골재 및 재생골재콘크리트의 Na₂SO₄침식에 의한 화학저항성에 관한 실험적 연구

김규용,이정율,박유신,강석표,김무한 忠南大學校 産業技術硏究所 1998 산업기술연구논문집 Vol.13 No.1

Now a day, the more Proportion of concrete used in practice is exposed to serious chemical attack Chemical reactions that involve formation of expansive products in hardened concrete can lead to certain harmful effects. In this study, it is to compare and investigate the chemical resistance of natural and recycled aggregate concrete by attacked Na₂SO₄according to W/C ratio. The result of this study is that the chemical resistance of recycled aggregate concrete is lower than natural aggregate concrete. But it is developed the chemical resistance by decreasing the W/C ratio.