http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
발목관절 중립위치와 발바닥 굽힘 위치간 스쿼트 운동 시의 하지근육 및 척추기립근 근활성도 비교: 예비 실험 연구
성하림,오세정,차용준 대전대학교 기초과학연구소 2019 自然科學 Vol.30 No.-
The purpose of this study was to compare muscle activity of lower extremity muscle and erector spine muscle between ankle joint neutral position and plantar flexion position. The muscle activity of the vastus medialis oblique, vastus lateralis, biceps femoris, and erector spinae were measured according to the two positions of ankle joint neutral position and ankle joint plantar flexion during squat exercise in 20 normal adults. The muscle activity of the vastus medialis oblique, vastus lateralis, biceps femoris, and erector spainae were significantly different according to the position of the ankle joint during squat exercise (p <.05). In the vastus medialis oblique, the ankle plantar flexion showed higher muscle activity than the ankle joint neutral position (73.9% of MVIC vs. 78.8 of MVIC). Ankle joint neutrality showed higher muscle activity than ankle joint flexion in the vastus lateralis, biceps femoris, and erector spinae (75.1% of MVIC vs. 69.4% of MVIC; 7.7% of MVIC vs. 6.7% of MVIC; 25.9% of MVIC vs. 21.9% of MVIC, respectively). The plantar flexion of the ankle joint during squat exercise is a more effective way to strengthen the vastus medialis oblique, but it is less effective than the ankle joint neutral position for strengthening the vastus lateralis, biceps femoris, and erector spinae.
( Ha Rim Kim ),( Jeong Mi Kim ),( Mi Seong Kim ),( Jin Ki Hwang ),( Kang Beom Kwon ),( Young Rae Lee ),( Hong Seob So ),( Sei Hoon Yang ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Non-small-cell lung cancer usually carries a dismal prognosis. Novel treatment approaches are clearly warranted. Alexidine dihydrochloride is known as an antimicrobial mouthwash in preventing plaque and gingivitis. In recent study, alexidine dihydrochloride has also known an apoptosis-promoting anticancer activity. Autophagy is known as an important regulatory mediator for cell survival or death and its important role in cancer. However, the role of autophagy and its underlying molecular mechanisms in alexidine dihydrochloride treated lung cancer cell lines have not been clarifi ed. The purpose of this study are to determine whether alexidine induces autophagy and to fi nd out the its mechanism in alexidine-treated A549 cells, we performed MTT assay, western blotting, FACS (acridine orange, Annexin V/PI) by using autophagy inhibitors. Cell viability decreased in alexidine-treated cells according to dose dependent manner. Expression of LC3 II and Beclin 1 were increased in alexidine-treated cells according to dose dependent manner. These fi nding indicated that alexidine induced autophgy. LC3 expression after Beclin 1, ATG 5 and ATG 12 siRNA pre-treatment is markedly decreased in alexidine treated A549 cells. After SB203580, P38 MAP kinase inhibitor pre-treatment, the proportion of acridine orange stain-positive cells decreased in alexidine treated A549 cells. Our results indicated that p38 MAP kinase may be a key regulator for Beclin 1-dependent autophagy.
( Ha Rim Kim ),( Jeong Mi Kim ),( Mi Seong Kim ),( Jin Ki Hwang ),( Kang Beom Kwon ),( Young Rae Lee ),( Hong Seob So ),( Sei Hoon Yang ) 대한결핵 및 호흡기학회 2014 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.118 No.-
Non-small-cell lung cancer usually carries a dismal prognosis. Novel treatment approaches are clearly warranted. Alexidine dihydrochloride is known as an antimicrobial mouthwash in preventing plaque and gingivitis. In recent study, alexidine dihydrochloride has also known an apoptosis-promoting anticancer activity. Autophagy is known as an important regulatory mediator for cell survival or death and its important role in cancer. However, the role of autophagy and its underlying molecular mechanisms in alexidine dihydrochloride treated lung cancer cell lines have not been clarified. The purpose of this study are to determine whether alexidine induces autophagy and to find out the its mechanism in alexidine-treated A549 cells, we performed MTT assay, western blotting, FACS (acridine orange, Annexin V/PI) by using autophagy inhibitors. Cell viability decreased in alexidine-treated cells according to dose dependent manner. Expression of LC3 II and Beclin 1 were increased in alexidine-treated cells according to dose dependent manner. These finding indicated that alexidine induced autophgy. LC3 expression after Beclin 1, ATG 5 and ATG 12 siRNA pre-treatment is markedly decreased in alexidine treated A549 cells. After SB203580, P38 MAP kinase inhibitor pre-treatment, the proportion of acridine orange stain-positive cells decreased in alexidine treated A549 cells. Our results indicated that p38 MAP kinase may be a key regulator for Beclin 1-dependent autophagy.
Development of Electrochemiluminescence-based Anti-drug Antibody Detection Assays
Ha-Rim Seo,Philyoung Lee,Min-Young Lee,Jae Hyeon Yoon,Kyungsoo Ha 한국분석과학회 2021 학술대회논문집 Vol.2021 No.11
Assays for the detection of anti-drug antibodies (ADAs) against Infliximab (IFX) and Adalimumab (ADL) have been mostly performed using an enzyme-linked immunosorbent assay. However, it was difficult to accurately detect anti-drug antibody due to the low sensitivity of the conventional methods. In this study, we employed the electrochemiluminescence method for quantification of anti-drug antibodies against infliximab and adalimumab in healthy subjects receiving a single and repeated dose injection up to 70 days. Relative light units of cut point for anti-IFX and anti-ADL were 91.4 and 93.2, and the calibration curve interval for anti-IFX and anti-ADL were set from 5 to 2000 ng/mL and 50 to 2000 ng/mL, respectively. Both anti-IFX and anti-ADL analyses were validated. Anti-IFX antibody was detected in one subject at 56 and 70 days, and anti-ADL antibody was detected in four subjects at 28, 56 and 70 days. In conclusion, we successfully developed an improved anti-drug antibody detection assay using an electrochemiluminescence method that could help us learn more about the relationship between immunogenicity and biological effects.