에탄올 생산 향상을 위한 발효저해물질 제거와 리그닌 유래 발효저해물질이 에탄올 발효에 미치는 영향

엄민 ( Min Um ),신경진 ( Gyeong-jin Shin ),이재원 ( Jae-won Lee ) 한국목재공학회 2016 목재공학 Vol.44 No.3

본 연구에서는 옥살산 전처리 바이오매스의 액상가수분해산물에 포함된 발효저해물질을 전기투석과 XAD 수지 처리하여 제거한 후 에탄올을 생산하였다. 전기투석 과정에서 아세트산은 대부분 제거되었으며(95.6%), 비이온성 발효저해물질(Total phenolic compound: TPC, 5-hydroxymethyl furfural: HMF, furfural)은 XAD 수지 처리에 의해 효과적으로 제거되었다. 전기투석과 XAD 수지 처리된 액상가수분해산물로 발효를 수행한 결과 XAD 수지의 침지시간이 짧을수록 에탄올 생산이 향상되었다. 최대 에탄올 생산은 발효 72시간 후 6.16 g/ℓ로 전기투석 후 액상가수분해산물을 XAD-4 수지에서 5분 침지하였을 때 나타났다. 리그닌 유래 발효저해물질 중 syringaldehyde는 저농도(1 mM, 2 mM)에서 에탄올 생산을 향상시켰으며 5 mM에서는 발효에 부정적인 영향을 주었다. 리그닌 유래 발효저해물질의 시너지효과를 확인하고자 합성배지로 발효를 수행하였으며, Syringaldehyde (1 mM)와 ferulic acid (1 mM) 합성배지를 이용하여 발효를 수행한 결과 syringaldehyde보다 ferulic acid의 영향으로 에탄올 생산이 감소했다. In this study, ethanol was produced from a biomass hydrolysate that had been treated by electrodialysis (ED) and Amberlite XAD resin to remove fermentation inhibitors. Most of the acetic acid (95.6%) was removed during the ED process. Non-ionizable compounds such as total phenolic compounds, 5-hydroxymethyl furfural, and furfural were effectively removed by the XAD resin treatment. Ethanol production was improved when the ED-treated hydrolysate was treated with XAD-4 resin for a short reaction time. The highest ethanol production from ED-treated hydrolysate was 6.16 g/ℓ (after 72 h of fermentation) when the treatment with XAD-4 resin was for 5 min. Among the ligninderived fermentation inhibitors tested, syringaldehyde in low concentrations (1 and 2 mM) in the hydrolysate increased ethanol production, whereas a high concentration (5 mM) inhibited the ethanol production process. A synthetic medium containing syringaldehyde and ferulic acid was prepared to investigate the synergistic effect of inhibitors on ethanol fermentation. Ethanol production decreased in the mixture of 1 mM syringaldehyde and 1 mM ferulic acid, implying that the effect of ferulic acid on ethanol fermentation is comparable to that of syringaldehyde.

컴퓨터 작업 시 의자 등받이 위치가 근육 활성도에 미치는 영향

김민우,변승진,이경은,정소영,조주영,최원자,최찬양,김경 대구대학교 특수교육재활과학연구소 2011 再活科學硏究 Vol.29 No.1

이 연구는 컴퓨터 작업시 의자 등받이 위치가 근육활성도에 미치는 영향을 알아보기 위해 36명의 근골격에 문제가 없는 성인 남녀가 참가하였으며 대상자들은 등받이가 뒤에 있는 의자와 등받이가 없는 의자, 등받이가 앞에 있는 우리들 의자를 무작위로 배정하고 동일한 컴퓨터 작업을 실시하게 하였다. 실험 중, 표면근전도기를 사용하여 의자에 따른 위등세모근, 머리널판근 그리고 척주세움근의 근활성도 변화를 측정하였다. 이 연구의 결과를 종합해보면 등받이가 앞에 있는 의자는 허리 근육의 부담을 줄이지만 목근육에 부담이 늘어나고 등받이가 뒤에 있는 의자와 없는 의자는 허리근육에는 다소 무리가 가지만 목근육에는 부담이 덜하다는 것을 알 수 있었다. 이러한 결과로 환자의 증상에 다라 컴퓨터 작업을 하는 동안 의자 등받이의 위치를 조절하여 치료에 도움이 될 수 있다고 생각되어진다. Objective : In this study, we checked and observed people's change of body muscle and muscle activity who do computer work on different chairs. We set three kind of chairs which one is a stool and another one is the back is fixed, and the other one is the back is fixed in front of chair. Subject : Accounting for 36 health men and women, we divided these people to 3 groups and put them on the three cases(position 1, position 2, position 3). We measured muscle activity of upper trapezius, Splenius Cervics, Erector Spinae from the groups by using surface electromyogram system. Methods : The standard of the electromyogram was 'reference voluntary contraction', and we carried out 'one way anova' to compare muscle activity of three groups. Results : In position 1, reference voluntary contraction of upper trapezius muscle activity was 123.2821%, Splenius Cervics was 141.7526%, and Erector Spinae was 254.5233%. In position 2, reference voluntary contraction of upper trapezius muscle activity was 132.9395%, Splenius Cervics was 141.7526%, and Erector Spinae was 246.6540%. In position 3, reference voluntary contraction of upper trapezius muscle activity was 190.6487%, Splenius Cervics was 270.2332%, and Erector Spinae was 182.1021%. Both upper trapezius and Splenius Cervics muscle activity of position 3 group was higher than the others groups. In position 1 group, Erector Spinae muscle activity was hight than the other groups. And either was position 2 group's(p<0.05). Conclusion : Therefore the chair which is the back is fixed in front is more comfortable for waist muscle but it's not good for neck. And the other chairs are better in waist muscle but not in neck muscle. So far, when we do a computer work, it's prefer to sit on the group 1&2's chairs to reduce neck muscle's stress and sit on group 3's chair to reduce waist muscle's stress.

외과계 집중치료실에서 입원한 환자에서 APACHE II score의 의의

이영재,이영준,박순태,하우송,정민화,권수인,조영현,권진용,최상경,손신 대한외상학회 1997 大韓外傷學會誌 Vol.10 No.1

This study attempts to evaluate the prognostic value of the APACHE II scoring system in patients of surgical intensive care unit(SICU) and to examine its usefullness in stratifying these patients according to their surgical risk and as a potential aid in making rational and objective treatment decision. The records of 161 patients admitted to the Department of Surgery, Gyeong-Sang National University Hospital between Jan. 1991 and Dec. l995 were reviewed. 1) Of a total of 161 patients, 87 were male, 74 were female, and male to female ratio was 1.71: 1. 2) This group was seen most commonly in the age group of 55-64 which occupied 30.3% of entire group. 3) The distribution of patients according to the etiology were 74 patients(46%) in trauma, 61 patients(37%) iin postop-complication, 26 patients(9%) in others. 4) The distribution of patients according to the APACHE II scores was 9 to 29 point in adm. Day and the most patients was located between 10 and 14 of 62 patient(39%). 5) The length of hospitalization was 9 days in survived patients and 8 days in non-survived patients. The length of hospitalization of 77% of patients in this study was below 10 days. 6) The death rate was 45.45%(73 patient), this patients according to APACHE II scores was 13 to 29, median 19.2 in adm. Day, the most patients was located 20 to 24(20%). Non of the survived patients had the APACHE II score of above 20 points, but all recovered patients were below 13 points. 7) The median APACHE II score in survived patients was 10.52 point at adm. Day and 6.55 point at 5 days after admission. And non-survived patients was 20.25 point at adm. Day and 30.48 point 5 days after admission(p$lt;0.001). 8) Among the patients with low APACHE II score (below 14 points), there was no mortality. But, there was mortality with APACHE II score of above l5 points. We conclude that APACHE II scoring system is useful in measuring the severity of acute disease and predicting the outcome.

Design and Implementation of a Chatbot System for Smart Stores

Min-Soo Kim,I-Na Kim,Jung-kwon Cho,Kyu-nam Lee,Min-young Kim,Do-Jin Choi,Jongtae Lim,Gyeong-Min Kim,Kyoung-Soo Bok,Jae-Soo Yoo 한국콘텐츠학회 2017 한국콘텐츠학회 ICCC 논문집 Vol.2017 No.12

Cultivating characteristics of Sangwhang mushroom, Phelinus linteus isolates in Korea

Gyeong-Jin Min,Ja-Yoon Kim,Hee-Wan Kang 한국버섯학회 2019 버섯 Vol.23 No.1

Comparisons of bioactive efficacies of artificially cultivated Sangwhang mushrooms, Phellinus linteus-P. baumii strains

Gyeong-Jin Min,Ja-Yoon Kim,Heewan Kang 한국버섯학회 2019 버섯 Vol.23 No.1

Genetic and bio-activity analysis a novel Phellinus linteus KACC 93057P having high mycelial growth rate

Gyeong jin Min,A min Kwak,Min jae Son,Sun ja Seok,Hee wan Kang 한국버섯학회 2015 버섯 Vol.19 No.1

Medical mushroom, Phellinus linteus and Phellinus baumii called as “Sanghwang” have cultivated in Korea. PL has been studied extensively for its extraordinary capacity of suppressing cancer or enhancing body immunity. The mycelial materials of PL have mainly been used as research samples worldwide because fruiting bodies was difficult to be artificially cultivated. Alternatively, P. baumii (variety, ‘Jangsu’) have been cultivated in Korea. However, fruiting body morphology of P. baumii is clearly different to that of PL. Generally, Phellinus spp. including P. linteus slowly grow on artificial medium such as Potato Dextrose Agar (PDA). In contrast, P. baumii strains were rapidly grown on the artificial media when compared to other Phellinus spp. and thus it was considerable that its mycelial growing ability can be acted as a factor for producing fruiting bodies. This study aimed to find Phellinus isolates having high mycelial growth rate. Five Phellinus isolates that show rapid growth rate on YGM medium were selected from 36 Phellinus isolates collected in Korea. They were identified on nucleotide sequences of rDNA-ITS region. Phellinus linteus strain and Phellinus spp. showing mycelial growth rate comparing to P. baumii were characterized on cultural and bioactive characteristics (antioxidant activity and immune activation).

A Study on the Cultivating Characteristics and Optimal Conditions for Mycelial Growth of Calocybe indica

Gyeong-Jin Min,Hye-sung Park,Eun-ji Lee,Chan-jung Lee 한국버섯학회 2020 버섯 Vol.24 No.1

Molecular Phylogenetic Classification and Cultivation Characteristics Analysis of wild-type collections of Ramaria Strains in Korea

Gyeong-Jin Min,Een-ji Lee,Hea-sung Park,Tai-moon Ha 한국버섯학회 2023 버섯 Vol.27 No.1

Sirtinol Treatment Influences Porcine Preimplatation Development Via Regulation of Autophagy and Apoptosis

Min Gyeong Kim,Duk Hyoun Kim,Jun Sung Lee,Su Jin Jin,Hoon Taek Lee 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2

In vitro production of pig embryos has been emerged for xenotransplantation due to physiological properties. Sirtuins play a crucial role in several cellular processes including DNA repair, apoptosis, cell cycle, and determining lifespan as NAD+ dependent class III histone deacetylase. Previous studies have shown that sirtuin inhibition induces embryonic developmental arrest and oxidative stress in porcine and murine models. Also, sirtuins regulate autophagy pathways through the deacetylation of ATG proteins and transcriptional factors. However, sirtuins-mediated mechanisms have not been examined in blastocysts. Therefore, we investigated the relationship between sirtuin inhibition and autophagy in porcine model. Embryos were cultured in 100 μM sirtinol (SIRT1/2 target inhibitor)-treated NCSU-23 media after in vitro fertilization. Treatment with sirtinol significantly decreased the rates of morula (21.34±1.84 vs. 11.89±2.01%), blastocyst formation (17.18±1.81 vs. 9.00±2.02%), and total cell number (50.80±1.47 vs. 37.71±1.79). Especially, expanded blastocysts (9.90±1.56 vs. 2.92±0.94%) were barely observed in sirtinol treated group. The level of Sirt2 expression was significantly decreased in sirtinol-treated group compared to control. Also, sirtinol induced autophagy by increasing LC3 transcript and LC3 protein in treatment group. Beclin1 and ATG5 expressions showed a slight increase in sirtinol-treated group. Finally, sirtuin inhibition by sirtinol showed significantly increased TUNEL indices (6.55±0.84 vs. 11.44±0.81) and fragmentation indices (0.33±0.05 vs. 1.40±0.30). Similarly, the level of BCL2L1 was lower, while the level of Caspase-3 expression was significantly elevated in the treated group compared to controls. Therefore, these results suggest that sirtuin inhibition may play an important role in porcine preimplantation development and embryo quality through regulation of autophagy and apoptosis pathways.